Tuyển chọn các chủng purpureocillium lilacinum có khả năng ký sinh tuyến trùng meloidogyne spp

BVTV - S6 2/2016

K6t qua nghien ciru khoa hoc
to evaluate the biocontrol potential of single spore
isolates of fungal entomopathogens, Journal of Insect
Science, 5(37): 1-10.
6. Sandhu S.S., Rajak R.C. and Agarwal
G.P.,1993. Studies on Prolonged Storage of Beauveria
bassiana Conidia: Effects of Temperature and Relative
Humidity on Conidial Viability and Virulence against
Chickpea Borer, Helicoverpa annigera, Biocontrol
Science and Technology, 3(1): 47-53.
7. Nguyen Thi Thanh, TrAn Ngoc Lan, Nguyen Thi
Thiry, 2011. Hieu lt,rc ph6ng triy sau khoang
(Spodoptera litura Fabr.) cua n6rn Isaria javanica
(Frider. & Bally) Samsom & Hywel-Jones, Top chi
Khoa hoc, Trisang Dai hoc Vinh, 40(4A): 84-89.

8. Vega F.E., Goettelb M.S., Blackwell M.,
Chandler D., Jackson M.A., Keller S., Koike M.,
Maniania N.K., Monzon A., Ownley B.H., Pell J.K.,
Drauzio E.N. Rangel D.E.N., Roy H.E., 2009. Fungal
entomopathogens: new insights on their ecology,
Fungal Ecology, 2: 149-159.
9. Vijayavani S., Reddy K. R. K. and Murthy
G.B.V.N., 2009. Pathogenicity of Beauveria bassiana
(Deuteromycotina: Euteromycotina: Hyphomycetes)
strains on Spodoptera litura (Fab.), Journal of
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Phan biOn: GS.TS.NCVCC. Pham Van Lam

TUYEN CHON CAC CHUNG Purpureocillium lilacinum CO KHA NANG
KY SINH TUYEN TRUNG Meloidogyne spp.
Selection of Indigenous Strains of Purpureocillium lilacinum
Able to Parasitize Root - Knot Nematode Meloidogyne spp.
Le Thi Thiry Nhi, Le Thi Mai Charm VO Thiry Duvng, Nguy6n Thi Thuy Duvng,
Pham Nguyen Dim Hoang, Otrang Hoa Xo va Pham HC.ru Nhuvng
Trung tam C6ng nghe Sinh hoc TP. HCM
Ngay chap nhan: 03.02.2016

Ngay nhan bai: 12.01.2016
Abstract

Purpureocillium lilacinum can parasitize egg-shells and females cuticle of root-knot nematodes Meloidogyne
spp. by mechanical and/or enzymatic activities. Ten indigenous isolates of P. lilacinum were isolated from black
pepper farms and National Parks infected with egg masses and female nematodes under in vitro conditions as an
initiative to control root-knot nematodes problem All isolates showed varying degrees in colonizing egg masses
and female nematodes. As observed by microscopy, these strains infected egg masses and female nematodes
by direct hyphae penetration. In the female nematodes bioassay, all strains demonstrated highly significant
colonization (>50%, P50.01) with BL16, BL10, XL1.2 showed 100% parasitism and highly radiating mycelia on
females. In egg masses parasitism test, these strains exhibited >30% parasitism with BL10, CM3.4, XL1.2,
BGM6.1 showed >80% parasitism on root-knot nematode eggs. Four indigenous strains of P. lilacinum namely
BL10, CM3.4, XL1.2, BGM6.1 demonstrated high and significant parasitism on egg masses and female
nematodes. So these strains should use as biological control agents for managing root-knot nematodes
Meloidogyne spp.
Keywords: Purpureocillium lilacinum, Meloidogyne spp., egg mass, female, parasitism.

1. DAT VAN DE
HO tieu hay tieu (Piper nigrum L.) la loaf gia vi
duac sir dung nhieu trong 5m thcic va duoo

canh tac cong nghiep tai nhieu vung. Viet Nam la
cuteng quoc xuat khautieu . Ian nhat the giai,
dOng vai trO binh on, dan dat gia Ca thi twang

24

the gioei. Tuy nhien, hau het cac man tieu & Viet
Nam deu bi nhiern tuyen trong Meloidogyne spp.,
lam cho tieu bi yang la chet cham. Them vao dO
con tao vet thuang cagiai de nhieu loci nam
benh trong Tat xam nhiern nhu: Fusarium spp.,
Phytophthora spp., Sclerotium rolfsii, Rhizoctonia
solani la nguyen nhan chinh gay dich chet nhanh,


Ket qua nghien ciru khoa hoc
chet cham hien nay, lam Om dang ke nang suet
h6 tieu cua Viet Nam. Purpureocillium lilacinum
(Thom) Luangsa-ard, Houbraken, Hywel-Jones &
Samson thuOc lap Sordariomycetes, b6
Hypocreales, ho Ophiocordycipitaceae, chi
Purpureocillium. P. lilacinum 16 nem co kha nang
1(9 sinh con cai va trirng tuyen trung duvc coi
nhu' mOt tac nhan kiem scat sinh hoc de phong
trU, tuyen trung (Luangsa-ard, 2011).
P. lilacinum co kha nang xam nhap kh6i trirng
Meloidogyne incognita, pha hi:1y phoi va xam
nhiem con cai twang thanh (Jatala va cs., 1979).
He khan ti nem xam nhap qua lap vo trirng nha
luc ca hoc va/hoac host dOng cua enzyme, qua

trinh lay nhiem bat dew vai sy' phat then cua
khan ti nem trong chat nen gelatine (Siddiqui va
Mahmood, 1997). Khan ti nem xam nhap thOng
qua cac 16 nho cua mang vitelline, gay ra sy' thay
doi tinh them cua v6 va sty na trirng, sau d6, Ian
Ong pha v& lap chitin va lap lipid lien ke.
P. lilacinum can, co kha nang san xuat serine
protease va mOt so chitinase pha hOy vo trirng,
cac enzyme nay cung giiip nem xam nhap qua lap
bieu bi cua tuyen trung (Khan va cs., 2006,
Moosavi va Zare, 2012). P. lilacinum la vi sinh vat
thuOc he nem det, phat then nhanh va it chlu anh
twang bai cac nem dOi khang, nem benh thu,c vat
(Pendse va cs., 2013). Do do, co the sir dung P.
lilacinum la tac nhan sinh hoc kiem soat tuyen
trung hieu qua, chinh vl the, chimg toi da fien
hanh thin nghiem kha nang kji sinh cua cac chung
P. lilacinum tren con cal va trirng tuyen trimg,
sang loc ra cac chung co hieu qua cao de phuc
vu cho muc dich tao the phern sinh hoc.
2. VAT LIEU VA PHUteNG PHAP NGHIEN cCni
2.1 Vat lieu nghien apu
Cac chOng P. lilacinum tir
giOng dia Trung
tam Cong nghe Sinh hoc da dvac dinh danh va
dvqc khao sat hopt tinh chitinase, protese, lipase,
chon ra 10 chung P. lilacinum co hopt tinh enzyme
toi vu nhet de khao sat kha nang kj/ sinh bao gom
10 chung: BL10, BL16, BS3.3 (dvac phan lap
mom QuOc gia Nam Cat Tien); BN5 (man QuOc

gia BO Gia Map), XL1.2, CM3.4, CM6.3 (wan tieu
tinh Song Nai) va BGM6.1, LN1.2, HQ5.1 (wan
tieu tinh Binh Phutec). Hai chung d6i chUng
Purpureocillium lilacinum
NBRC5350 (PL),
Pochonia chiamydosporia NBRC10314 (PC) mua
tip ngan hang giOng dia Nhat Ban.
Con cai va kh6i (rung tuyen trung
Meloidogyne spp. duac tech va thu nhan tip cac

BVTV - S6 2/2016
not seri tren re cay tieu thu thep a mOt so wan
tieu thuOc tinh Hong Nai va tinh Binh Phvac.
2.2 Phu'o'ng phap nghien can'
Chan bj mOi twang WA b6 sung 0,1 g/I
chloramphenicol, help Whir trung, phan ph6i deu
dia Petri vO trung. Con cai va kh6i VON tuyen
bao guar' trong
King sau khi tech tir re cay
['vac muOi sinh ljt va dvac kh6, trung bAng cach
rira 3 len vai rarac cat v6 trung. Cey nem vao
giu,a dia Petri mOi tru'ang, dung kim hOt dat con
cai tuyen trung va kh6i trivng xung quanh nem,
cach tam dia 1 cm, nu6i cay a nhiet d6 25 ± 2°C.
Thi nghiem dvac 1)6 tri theo kieu hoar) toan ng5u
nhien, vai 3 len lap lei, mOi dia 8 con cai hoac 8
khoi trirng tuyen King (Khan va cOng sy', 2006;
Pau va cOng skr, 2012).
Chi tieu theo dOi: Theo dal
phat then cua

khan ti nem, do &rang kinh tan nem sau al.& 2
ngay nu6i cay, tinh tjf le con cai, kh6i trirng tuyen
trung bi ky sinh, quan sat su' xam nhap cua
khan ti nem vac) tuyen trung !Deng cach nhuOm
vai lactophenol blue.
3. KET QUA VA THAO LUAN
Sau thi nghiem dinh tinh he enzyme ngoai
bao (chitinase, protease va lipase), chon loc
dvac 10 chung nem BS3.3, BL10, BL16, XL1.2,
CM3.4, CM6.3, BN5, BGM6.1, LN1.2 va HQ5.1
nhet. Chung tOi tiep tuc danh gia kha nang
ky sinh tren trirng va con cai tuyen truing
Meloidogyne spp. trong phOng thi nghiem.
Kha nang k9 sinh tuyen (rung nhanh hay
cham phu thuOc vao
phat then nhanh hay
cham cua cac chUng nem tren mOi tru'ang thach
nvac cat. Trong thi nghiem nay, khi P. lilacinum
dat &rang kinh khoang 2 cm thi khan ti nem bet
deu tier) XCJC vai con cai. Nhin vac bang 1, to they
&yang kinh cua chung BL16 phat then nhanh
nhet, khan ti nem cham den con cai chi sau 4
ngay, con cac chung nem khac thi phat trien
cham hen nen &rang kinh dpt 2 cm sau 6 ngay,
khan ti nem mai cham vao con cai. Ket qua nay
twang ty' nhu' trong nghien c0,u cua Pau va cs.
(2012) khi thin nghiem dat con cai vao dia P.
lilacinum de moc 5 ngay, thi sau 6 ngay khan ti
nem bat deu xam nhap vao con cai Meloidogyne
incognita, ben canh d6 Khan va Ong sy' (2006)

they sau 4 ngay khan ti nem bao quanh con cai.
Kha nang kj/ sinh con cai cung phy thuOc vao
kha nang tiet enzyme ngopi bao chitinase va
lipase cua cac chung nem (Pau va cOng
25


Ket qua nghien CCFU khoa hoc

BVTV - S6 2/2016

nang ky sinh con cal cua tirng chung nim, chung
toi theo d6i s6 tj, 10 (%) con cai bi ky sinh cua
tiyng chUng.

2012), bang chirng la nhang chung CO khuen lac
phat then nhanh la nhO,ng chUng dipac chon co
chitinase manh (BL10, XL 1.2, CM3.4) va lipase
manh (BL16, XL1.2). De danh gia chinh xac kha

Bang 1. Trung binh du•o'ng kinh cua cac chung nAm P. lilacinum chon loc dtro,c va chung ddi
chirng Purpureocillium lilacinum (PL), Pochonia chlamydosporia (PC)
tren dia WA khao sat kha nang ky sinh con cai Meloidogyne spp.

Sn

Dirteng kinh tan n6rn (cm)

1

BS3.3

1,11

4 ngay
1,41

2

BL10

1,10

1,40

Chung

2 ngay

14 ngay
3,83i

2,24

8 ngay
2,66

10 ngay
3,24

12 ngay
3,59

2,27

2,91

3,80

4,55

5,15a

4,40

4,80e

6 ngay

3

BL16

1,70

2,16

3,21

3,30

3,58

4

CM3.4

0,76

1,09

1,58

2,26

2,78

3,29

3,75b

4,21

4,72f

5

CM6.3

1,11

1,43

2,10

2,86

3,61

6

XL1.2

0,96

1,31

2,20

2,95

3,85

4,25

5,00b

3,90

4,62g

4,85d

7

BN5

0,89

1,13

2,90

8

BGM6.1

1,05

1,46

1,91
2,14

3,50

2,91

3,54

4,09

0,91

1,19

2,00

2,45

2,80

3,20

3,51j

9
10

HQ5 1
LN1.2

0,85

0,97

1,80

2,49

2,86

3,43

3,91h

11

PL

1,10

1,59

2,22

3,35

3,74

4,31

4,96c

12

PC

0,80

1,09

1,61

2,01

2,55

2,83

3,40k

th6ng ke 6. mac xac suet p = 0,01)
coy
(Cac chic cai trong cuing met c6t khOng cling 1c)", thi khac bie t COnghia

TY LE (%) CON CA1131 KY SINH
[3

2000
‘
1.

I 111NG

Hinh 1. Bieu d6 so sanh ty Ie con cai bi kyr sinh
bai cac chung nam P. lilacinum chon loc cityac
va chung doi chUng Purpureocillium lilacinum
(PL), Pochonia chlamydosporia (PC)
(cac chi' cal tren c6t gia tri khOng cOng ky tv. thi khac
Piet c6 )", nghia thOng Ice a tn6c xac suet p = 0.01).

Du'a vao bleu dO 1, they rang sau 14 ngay,
tit ca cac chUng nim khao sat deu ky sinh tren

26

50% con cai thi nghiem, trong do, dang ch0 9
nhat la cac chung BL16, BL10, XL1.2 da ky
sinh het 100% con cai, ke den la cac chUng
CM3.4, BGM6.1, PL de ky sinh tren 80% con
cai. Ket qua nay cOng tuang &yang vai su,
phat trier) &rang kinh cua cac chUng nim tren
m6i truteng WA, chung co kha nang ky sinh
con cai cao cung la chUng co &Pang kinh 16'n
hen cac chung khac.
Quan sat dual kinh hien vi soi n6i, chOng tOi
nhan they khuen ti P. lilacinum co kha nang dam
xuyen triic tier) qua lOp bleu bi cua con cal giOng
voi bao cao cua Khan va cOng su' (2006) va Pau
va cOng su' (2012), khac vai bao cao dia Jatala
va cOng sty (1979) P. lilacinum xam nhiem con
cai Meloidogyne thong cac 16 ma dia co' the nhu,
hau mon va am hO. Hinh 2B cOng chung minh
khuen ti nim (bit mau xanh cua lactophenol
blue) dam xuyen tryc tiep qua lap bleu bl cua
con cal ma khong can cac I6 ma tren ca the, dieu
ca
nay ca the do ca the tiet enzyme va ap
hoc cua sqi nim giup P. lilacinum dam xuyen
tru'c tiep qua lap bleu bi.

Ket qua nghien ct:Pu khoa hoc

BVTV - S6 2/2016

Hinh 2. Con cal c6 nem ky sinh chup dutri kinh hien vi huynh quang d6 ph6ng dai 400 In
A: D6i chung: con cai khOng co nem ky sinh, B: Khuen ti nam (mCli ten do) xam nhep qua kip bleu bi
con cai, C: Khuan ti nam (mill ten de)) ben trong ca the con cal.

A
Hinh 3. Con cai Meloidogyne spp. bi khuen ti nem (mai ten d6) ky sinh
chup diroi kinh soi not d§ ph6ng dai 45 In
Con cai bi nam ky sinh: sau 7 ngay (A), sau 14 ngay (B).
Kha nang ky sinh khoi trCyng Meloidogyne spp.
cua cac chung nem P. lilacinum yeu hart so vai kha
nang ky sinh con cai. Khi nuoi nam tren mOi tnyang
WA co dat cac kh6i trirng Meloidogyne spp. thi
chung phat then cham ham Bang 2 the hien ket qua
sau xtr ly thong ke dung kinh cua cac chung nem
P. lilacinum sau cap ngay thi nghiem.
Kat qua khao sat kha nang ky sinh khOi trCyng
tuyen trimg Meloidogyne spp. cua cCia 10 chCing
nam da chon cho thay, cht.ing BL16 CO kha nang
phat then nhanh nhat tren mOi biking WA CO dat
cac khoi trirng Meloidogyne spp. (dt.rang kinh nem
dat 2,113 cm sau 6 ngay nuoi cay). Den ngay thir
8, mot so cht.ing nem co dutng kinh dat khoang 2
cm (BS3.3, BL10, CM6.3, XL1.2, BGM61), thi
khuan ti nem cham den khoi trtrng. Khan va cong

sir (2006) dat trirng tuyen trung Meloidogyne
javanica vac) nem P. lilacinum d5 nuoi cay 5 ngay

thi tang bi nhiem sau 10 ngay, con thi nghiem cua
Pau va cong sir (2012) cho thay trCyng Meloidogyne
incognita &Ito lay nhiem vai 10 bao tir P.
lilacinum/ml thi sau 5 ngay truing bi ky sinh, cac thi
nghiem nay Mitt tien hanh tren cac trirng rieng le
duac tach rod tCY kh6i trirng nen thai gian bi ky sinh
nhanh han. Kha nang xam nhap trirng con tuy thu6c
vao kha nang tiet enzyme ngoai bao protease,
lipase, CO the giOng co hoat tinh protease manh se
CO kha nang ky sinh khOi trCyng cao. Do dO, chung
BL16 tuy ban deu phat then nhanh (dt.yang kinh dat
2,113 cm sau 6 ngay nuoi cay) nhung khi so sanh
vai thi nghiem ky sinh con cai thi &yang kinh tan
nam trong thi nghiem ky sinh khoi trirng nho hurl, do
chung nay protease yeu han. Va khi so sanh vai cac
chiing nam khac trong 10 thi nghiem thi BL16 phat
then cham han chCmg BL10 va BS3.3 (BL10 va
BS3.3 co vOng protease manh).

27


BVTV - S6 2/2016

Ket qua nghien CCM khoa hoc

Bang 2. Trung binh duVng kinh cua cac chUng nam P. lilacinum chon loc duvc va

chUng d6i chipng Purpureocillium lilacinum (PL), Pochonia chlamydosporia (PC)
tren dia WA khao sat kha nang ky sinh tui tripng Meloidogyne spp.
arang kinh tan nam (cm)
STT

ChCing

2 ngay

4 ngay

6 ngay

8 ngay

10 ngay

12 ngay

14 ngay

1

BS3 3

1,10

1,31

1,89

2,24

2,49

3,01

3,45b

2

BL10

1,10

1,40

1,90

2,51

2,80

3,39

3,80a

3

BL16

1,61

1,78

2,11

2,15

2,19

2,39

2,61f

4

CM3.4

0,76

1,13

1,34

1,71

1,81

2,05

2,32c

5

CM6.3

1,05

1,29

1,71

2,00

2,10

2,45

2,53h

6

XL1.2

0,94

1,30

1,79

2,29

2,65

2,84

3,05d

7

BN5

1,05

1,22

1,40

1,70

1,77

2,10

2,24i

8

BGM6.1

0,91

1,16

1,50

2,11

2,22

2,49

2,80e

9

HQ5.1

0,91

1,05

1,45

1,76

1,90

2,13

2,251

10

LN1.2

0,81

1,10

1,38

1,69

1,85

2,20

2,55g

11

PL

1,10

1,40

1,39

1,92

2,00

2,15

2,25i

12

PC

0,81

1,03

1,19

1,49

1,55

1,70

1,80j

(Cac chir cal trong cung nxit c6t khOng cang ky ty'thi khac biet coy nghia th6ng ke a muc xac suet p= 0,01)

TV LE (%)TRO.NG 13!

SINH

(I

I

oN• c-;• 4s,

Hinh 4. Bieu dO so sanh tY,le khOi tru'ng bi
ky sinh h&j cac chung nam P. lilacinum
va chUng dot chung Purpureocillium lilacinum
(PL), Pochonia chlamydosporia (PC)
sau 14 ng5y thi nghiO.n
(cac chit' cal tren cot gia tri, khOng cang ky tur thi khac
biet coy nghia thong kO a m6c xac suit p = 0,01).
Kha nang ky sinh khoi trOng cLia cac chung
nam P. lilacinum dircyc chan kern han so vai ky
sinh con cai. Bleu d6 hinh 4 cho they, cac khoi
hi:mg bi ky sinh bai cac chUng nam sau 14 ngay

28

deu ten 30% (phan tram con cai bi ky sinh sau 14
ngay ct.ia cac chOng nam deu tren 50%). Trong do,
cac chCing nam CO kha nang ky sinh triing cao la
BL10, CM3.4, XL1.2, BGM6.1 (dat tren 80%) (cac
chung nay cang cO .duang kinh khuen lac Ian). Thi
nghiem nay cho thay, cac chimg nam P. lilacinum
phan lap &roc CO kha nang ky sinh tCti triing va con

cai Meloidogyne spp. hieu qua han so vai cac
chung nam doi chung P. lilacinum va cao han
chung P. chlamydosporium, trong thi nghiem cCia
Eapen va tong su' (2005) P. lilacinum cOng co kha
nang ky sinh con cai va trtYng cao han P.
chlamydosporium.
Trong thi nghiem nay chCing t6i chi tap trung
vao danh gia kha nang ky sinh con cai va kh6i
trimg tuyen trung cOa cac chung P. lilacinum
phan lap thn. Khi nam bao vay toan b6 khoi
trCrng va pha hay trCrng, chi co mot vai trUng na
thanh J2, tuy nhien nhin hinh 5B va 5C nhan they
khuen ti P. lilacinum con qu6n quanh ca 6u trung
J2 no' tit' khoi trtYng. Khan va Ong su' (2006)
cang da ket luan P. lilacinum co kha nang ky sinh
Au trung J2, vi vay co the sir dung P. lilacinum la
tac nhan sinh hoc kiem scat hieu qua tuye'n trung
Meloidogyne spp.


K6t qua nghien ciru khoa hoc

BVTV - S6 2/2016

Hinh 5. KhOi trirng Meloidogyne spp. bI nAm P. Illacinum kjt sinh
(chyp dunfri kinh soi not dO phony 41 45 Ian)
a: Nam meri titip xuc voi kh6i tn"mq, b: N6m xhm nh$p vao kh6i trirng,
c: NAm phat trien fret, kh6i trepng.

Hinh 6. KW& tru'ng Meloidogyne spp. va Au Wing J2 bi nAm P lilacinum kjt sinh

a: KhuAn ti nam bao vay va dam xuyen toan b¢ tui tram, b: KhuAn ti nArn qu.in quanh J2 va tin Ming,
c: KhuaRn ti n6m quAn quanh J2.
4. KET LUAN VA DE NGHI
"fir cac ket qua thi nghiem, chCing toi chon
ditivc 4 chOng nem CO kha nang ky sinh tuyen
trung hieu qua la BL10, CM3.4, XL1.2 va BGM6.1,
cac chung nay vira CO kha nang k9 sinh con cal
vire co kha nang ky sinh khoi trirng tuyen trung.
Can khao nghiem them kha nang ky sinh au
King tuy4n King cua cac chung nem, nang cao
hieu qua kiem soat tuyen triang. Cac chung nem
c6 kha nang ky sinh cao, tiep tic thin nghiem trcrc
tier) Van cay tong de chon &Ivo nhCrng chung
CO kha nang kiem soat tuyen trung t6i Lru, Ur do
Crng dyng tato cha .pharn sinh tux CO kha nang
phong trir tuyen trung hieu qua.

TAI LIEU THAM KHAO
1. Eapen S.J., Beena B. and Ramana K.V., 2005.
Tropical soil microflora of spice-based cropping
systems as potential antagonists of rootknot
nematodes. J. Invertebr Pathol., 88: 218-225.
2. Jatala P., Tenbach K.R. and Bocangel M.,
1979. Biological control of Meloidogyne incognita acrita
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Phan bie-n: TS. Dao Thi HAng

DANH GIA HIED 14C PHONG TRU' RAY NAU HAI LUA
(Nilaparvata lugens Stal.) CCM MOT S6 THUOC TRU' SAU
Bio-Efficacy Evaluation of Some Insecticides Against Rice BPH
(Nilaparvata lugens Stal.)
Phung Minh Loci, TrAn Thanh Tung', Le Thi Dieu Trang2, H6 Thi Thu Giang3 va Taro Adati4
Ngay ch6p nhOn: 17.02.2016

Ngay nhan bat: 23.12.2015
Abstract

Brown planthopper (BPH), Nilaparvata lugens is a serious pest affecting rice in Southeast Asia countries including
Vietnam. To deal with this pest problem, farmers rely mainly on chemical pesticide use. High pressure of pesticide use
has led to increase in pesticide tolerability of BPH. This study aims to evaluate the bio-efficacy of some insectides which
had been being used for a long period of time against BPH on rice field in Tien Giang province, Mekong Delta. The
insecticides used in this study were based on the recent survey on farmers pesticide use practices in the Mekong
Delta. In this study, two experiments were conducted in Winter - Spring (2014-2015) and Summer — Autumn (2015)
season. The trials were carried out by using Randomized Complete Block Design (RCBD) with 8 treatments (7 active
ingredients including buprofezin (Applaud 10WP, 1.0 kg/ha), imidacloprid (Confidor 100SL, 0.4 Uha), dinotefuran (Oshin
20WP, 0.3 kg/ha), sulfoxaflor (Closer 500WG, 0.6 kg/ha), pymetrozine (Chess 500WP, 0.3 kg/ha), fipronil (Regent
800WG, 0.04 kg/ha), fenobucarb (Bassa 50ND, 1.0 I/ha), and one untreated control). The trials were replicated 3 times
with 52.5 m2 per plot. The efficacy of pesticides used in this study was determined based on the number of insect
individuals (nymphs and adults) which monitored before and 1, 3, 7, 10, 14 days after treatment.
Results indicated that, most of the tested insecticides including buprofezin, imidacloprid, fenobucarb, fipronil
and sulfoxaflor had low efficacy on BPH (less than 65%). Only two active ingredients tested (dinotefuran and

pymetrozine) were still effective in controlling BPH (higher than 70% efficacy). The decline in pesticide efficacy in
this study may be due to overuse of these pesticides for a long time, leading to high selection pressure on insects.
As a result, the development of pesticide resistance may occur.
Keywords: Insecticides, bio-efficacy, rice pests, brown planthopper.

1. DAT VAN DE
Ray nau (Nilaparvata lugens) la mOt trong
nhirrig dich hai quan trong nhat gay hai

1. Trung tam KD&KN thuOc. BVTV phia Nam.
2. Liking Dai hoc Nong lam Tp. HO Chi Minh.
3. Floc vien Nong nghiep Viet Nam.
4 Dai hoc Nong nghiep Tokyo, Nhat Ban.

30

nhieu niybc Chau A trong do CO Viet Nam
[20,10]. Rey nau co the gay hai trcyc tier) tren cay
10a th6ng qua chich hOt, chOng cOn gay hai gian
tiep vi la vac to truyen benh vi rut gay benh yang
lOn va lun xoan la cho cay 10a.
CO nhieu loci thu6c trir sau duvc nOng dan
sir dung phOng trir ray nau nhung nang suat 10a
van bi Om do dich hai nay gay ra. Ap 11,FC sCr
dung thu6c trir sau cao de dan den hien tuvng