Bsi bs en 00599 1 2009 + a1 2013

BRITISH STANDARD

Durability of wood
and wood-based
products — Efficacy
of preventive wood
preservatives as
determined by
biological tests
Part 1: Specification according to
use class

ICS 71.100.50

NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW

BS EN 599-1:2009
+A1:2013


BS EN 599-1:2009+A1:2013

National foreword
This British Standard is the UK implementation of
EN 599-1:2009+A1:2013. It supersedes BS EN 599-1:2009 which is
withdrawn.
The start and finish of text introduced or altered by amendment is
indicated in the text by tags. Tags indicating changes to CEN text
carry the number of the CEN amendment. For example, text altered by
CEN amendment A1 is indicated by !".
The UK participation in its preparation was entrusted to Technical

Committee B/515, Wood preservation.
A list of organizations represented on this committee can be obtained
on request to its secretary.
This publication does not purport to include all the necessary
provisions of a contract. Users are responsible for its correct
application.
Compliance with a British Standard cannot confer immunity
from legal obligations.

This British Standard
was published under
the authority of the
Standards Policy and
Strategy Committee on 31
January 2010
© The British Standards
Institution 2014. Published
by BSI Standards Limited
2014

ISBN 978 0 580 82821 8

Amendments/corrigenda issued since publication
Date

Comments

31 January 2014

Implementation of CEN amendment A1:2013

EUROPEAN STANDARD

EN 599-1:2009+A1

NORME EUROPÉENNE
EUROPÄISCHE NORM

December 2013

ICS 71.100.50

English Version

Durability of wood and wood-based products - Efficacy of
preventive wood preservatives as determined by biological tests
- Part 1: Specification according to use class
Durabilité du bois et des matériaux dérivés du bois Efficacité des produits préventifs de préservation du bois
établie par des essais biologiques - Partie 1: Spécification
par classe d'emploi

Dauerhaftigkeit von Holz und Holzprodukten - Wirksamkeit
von Holzschutzmitteln wie sie durch biologische Prüfungen
ermittelt wird - Teil 1: Spezifikation entsprechend der
Gebrauchsklasse

This European Standard was approved by CEN on 20 June 2009 and includes Amendment 1 approved by CEN on 21 October 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2013 CEN

All rights of exploitation in any form and by any means reserved
worldwide for CEN national Members.

Ref. No. EN 599-1:2009+A1:2013 E


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Foreword
This document (EN 599-1:2009+A1:2013) has been prepared by Technical Committee CEN/TC 38 “Durability
of wood and wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by June 2014, and conflicting national standards shall be withdrawn at
the latest by June 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document includes Amendment 1 approved by CEN on 21 October 2013.
This document supersedes EN 599-1:2009.
The start and finish of text introduced or altered by amendment is indicated in the text by tags .
Significant technical differences between this edition and EN 599-1:1996 are as follows:
—

The scope now emphasises that this standard is not a performance standard but a standard for
characterising the efficacy of wood preservatives, based on data derived from the biological tests that it
specifies, supplemented by, in certain cases, data from field tests. This European Standard provides a
basis for establishing preservative retentions for timber when specified in conjunction with EN 351-1
taking into account necessary local considerations. It points out that at present not all natural ageing
factors which may affect the stability of active ingredients for wood preservatives can be assessed by
standardized methods but have nevertheless to be taken into account in the development of preservative
products capable of being effective in service. It also allows for preservatives that have been used
successfully and legally before the standard was to have their efficacy confirmed in accordance with the
general principles of the standard (so called “grandfather-clause”).

—

This document now provides for the use of data from CEN/TS 839, in specific cases, as a method for the
assessment of preservatives designed for superficial applications.

—

In Annex A “Guidance on re-testing after making variations in product formulation”, requirements have
been clarified by the provision of diagrams.

—

Production control has been withdrawn from Clause 9 and transferred to a new informative annex (now
Annex B).

—

A new normative annex on the validity of test results from former standards after their revision has been
added (now Annex H).

EN 599 consists of two parts. Part 2 (EN 599-2, Durability of wood and wood-based products — Performance
of preventive wood preservatives as determined by biological tests — Part 2: Classification and labelling) will
be revised later once the exact requirements of the Biocidal Products Directive have been finalised.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

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BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Contents

Page

Introduction .........................................................................................................................................................4
1

Scope ......................................................................................................................................................5

2

Normative references ............................................................................................................................5

3
3.1
3.2

Terms, definitions, abbreviations and symbols .................................................................................7
Terms and definitions ...........................................................................................................................7
Abbreviations and symbols ..................................................................................................................9

4

Assignment to use class(es) ............................................................................................................. 10

5
5.1
5.2

Efficacy ................................................................................................................................................ 11
Principal requirements ....................................................................................................................... 11
Additional requirements in Table 1 to Table 5 ................................................................................. 11

6

6.1
6.2
6.3
6.4
6.5

Tests for efficacy according to use class ........................................................................................ 15
Use class 1 .......................................................................................................................................... 15
Use class 2 .......................................................................................................................................... 16
Use class 3 .......................................................................................................................................... 16
Use class 4 .......................................................................................................................................... 17
Use class 5 .......................................................................................................................................... 18

7

Derivation of critical values ............................................................................................................... 18

8

Marking ................................................................................................................................................ 19

9
9.1
9.2

Product identification ......................................................................................................................... 19
Type testing ......................................................................................................................................... 19
Identity of the preservative product.................................................................................................. 19

Annex A (informative) Guidance on re-testing after making variations in product formulation.............. 27

A.1

Introduction ......................................................................................................................................... 27

A.2

No requirements for new biological testing ..................................................................................... 27

A.3

Requirement for minimum new biological testing .......................................................................... 28

A.4

Requirement for full new biological testing ..................................................................................... 32

Annex B (informative) Production control ..................................................................................................... 33
B.1

Procedure ............................................................................................................................................ 33

B.2

Records ................................................................................................................................................ 34

Annex C (informative) Methods of application .............................................................................................. 35
Annex D (informative) Type of wood .............................................................................................................. 36
Annex E (informative) Artificial ageing procedures ...................................................................................... 37
Annex F (informative) Significance of specific biological agents ............................................................... 38
Annex G (informative) Minimum efficacy requirements ............................................................................... 39

Annex H (normative) Validity of test results from former standards after their revision ......................... 40
Bibliography ..................................................................................................................................................... 41

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BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Introduction
This part of EN 599 is one of a series and should be used in conjunction with EN 599-2, EN 335-1 and
EN 335-2, which describe the service conditions for treated wood in terms of use classes, and EN 351-1,
which describes a classification system for preservative treated wood in terms of preservative penetration and
gives guidance on classes for retentions. The need for wood preservatives depends in part on the natural
durability of the wood and therefore this part of EN 599 should also be used in conjunction with EN 350-1,
EN 350-2 and EN 460.

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EN 599-1:2009+A1:2013 (E)

1

Scope

This part of EN 599 specifies for each of the five use classes defined in EN 335-1, the biological tests required
for evaluating the efficacy of wood preservatives for the preventive treatment of solid timber, together with the
minimum ageing tests required for the respective use class. It provides the method for calculating the critical

value of a preservative. The critical value is the value that shall be used to calculate the recommended
retention of the preservative appropriate for specific service conditions. The critical value is not necessarily the
recommended retention or the minimum retention level for the preservative. The wide range of hazards,
exposure conditions and service life requirements across Europe make it necessary to allow for local
considerations in the calculation of the required preservative retention; EN 351-1 provides for the critical value
to be adjusted to take account of these factors.
This part of EN 599 is applicable to all wood preservative products supplied for application in liquid form for
the preventive treatment of timbers (structural and non-structural) against wood-attacking fungi, woodattacking insects and marine borers as described in EN 335-1. However, it is applicable to products for
preventive treatments against fungi causing disfigurement (blue stain) of wood in service if this forms part of
the overall preventive efficacy of the product.
This part of EN 599 does not necessarily take into account all the factors which may affect the stability of
active ingredients in preservative treated wood. These factors include ultra-violet light and microbiological
agencies capable of degrading components of the preservative. Such factors are an integral part of exposure
in field trials but are subject to natural variation and their impact is not directly assessed in the field trial
methods included in this standard. Methods are in development to assess the impact of these factors but are
not finalised and cannot be included in this revision of this part of EN 599. Because such factors could, in
service, significantly affect the active ingredient’s suitability for its intended purpose, the manufacturer/
producer is expected to ensure and be able to provide evidence that its stability, at the recommended
retention of the preservative product, has been adequately assessed.
This part of EN 599 is not applicable to wood preservative products supplied for application as pastes, solids
or in capsule or gaseous form because these cannot be tested without modification of the biological tests cited
in this standard. It does not apply either to wood preservative products for remedial (curative) treatments, to
those applied to prevent fungi causing sap stain on green (unseasoned) timber or to those applied solely to
prevent fungi causing disfigurement (blue stain) of wood in service.
NOTE 1
The nature of the laboratory and field tests required in this part of EN 599 to demonstrate efficacy of a wood
preservative are such that the time required generating the data is many months or years, depending upon the properties
of the wood preservative and the use class in which the treated wood is to be exposed.
For preservative products which have already been placed on the market without significant formulation variation (see
Annex A) before the end of 1990 and which can demonstrate a record of having been used lawfully and successfully in

accordance with local technical traditions during this period, national standards bodies or independent authorities
nominated by them, may declare critical values for use within their territories.
NOTE 2

2

For re-testing after making variations in product formulation, guidance is given in Annex A.

Normative references

The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN 20-1, Wood preservatives - Determination of the protective effectiveness against Lyctus Brunneus
(Stephens) - Part 1: Application by surface treatment (laboratory method)

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EN 599-1:2009+A1:2013 (E)

EN 20-2, Wood preservatives - Determination of the protective effectiveness against Lyctus brunneus
(Stephens) - Part 2: Application by impregnation (Laboratory method)
EN 46-1, Wood preservatives - Determination of the preventive action against Hylotrupes bajulus (Linnaeus) Part 1: Larvicidial effect (Laboratory method)
EN 46-2, Wood preservatives - Determination of the preventive action against Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory method)
EN 47, Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus)
- (Laboratory method)
EN 49-1, Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De
Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)

EN 49-2, Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De
Geer) by egg-laying and larval survival - Part 2: Application by impregnation (Laboratory method)
EN 73, Wood preservatives - Accelerated ageing tests of treated wood prior to biological testing - Evaporative
ageing procedure
EN 84, Wood preservatives - Accelerated ageing of treated wood prior to biological testing - Leaching
procedure
EN 113, Wood preservatives - Test method for determining the protective effectiveness against wood
destroying basidiomycetes - Determination of the toxic values
EN 117, Wood preservatives - Determination of toxic values against Reticulitermes species (European
termites) (Laboratory method)
EN 118:2005, Wood preservatives - Determination of preventive action against Reticulitermes species
(European termites) (Laboratory method)
EN 152, Wood preservatives - Determination of the protective effectiveness of a preservative treatment
against blue stain in wood in service - Laboratory method
EN 252, Field test method for determining the relative protective effectiveness of a wood preservative in
ground contact
EN 275, Wood preservatives - Determination of the protective effectiveness against marine borers
EN 330, Wood preservatives - Field test method for determining the relative protective effectiveness of a wood
preservative for use under a coating and exposed out of ground contact: L-joint method
EN 335-1, Durability of wood and wood-based products — Definition of use classes — Part 1: General
EN 351-1, Durability of wood and wood-based products - Preservative-treated solid wood - Part 1:
Classification of preservative penetration and retention
EN 599-2, Durability of wood and wood-based products - Performance of preventive wood preservatives as
determined by biological tests - Part 2: Classification and labelling
ENV 807:2001, Wood preservatives - Determination of the effectiveness against soft rotting micro-fungi and
other soil inhabiting micro-organisms
CEN/TS 839, Wood preservatives - Determination of the protective effectiveness against wood destroying
basidiomycetes - Application by surface treatment

6

BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

EN 1001-2:2005, Durability of wood and wood based products - Terminology - Part 2: Vocabulary

3
3.1

Terms, definitions, abbreviations and symbols
Terms and definitions

For the purposes of this document, the terms and definitions given in EN 1001-2:2005 and the following apply.
3.1.1
active ingredient(s)
a.i
individual chemical compound or compounds included in a wood preservative to give it specific activity against
biological agents of deterioration
[EN 1001-2:2005, 4.01]
3.1.2
analytical zone
a.z
part of the treated wood which is analysed for assessing the retention requirement (r.r.)
NOTE
The analytical zone is taken from the lateral surfaces of the treated wood. The depth to which sampling is
required will depend on the species of wood being analysed and the treatment level concerned.

[EN 1001-2:2005, 4.03]
3.1.3

biological reference value
b.r.v
amount in grams per square metre or kilograms per cubic metre of a wood preservative (as product) found to
be effective in test in preventing attack by the particular biological agent being tested
[EN 1001-2:2005, 4.06]
3.1.4
critical value
c.v
value equivalent to the highest b.r.v. (in grams per square metre or kilograms per cubic metre) obtained from
all the biological tests carried out in accordance with this part of EN 599 for any given use class
[EN 1001-2:2005, 4.18]
3.1.5
co-formulant
any ingredient (other than an active ingredient) in a formulated wood preservative product
[EN 1001-2:2005, 4.14]
3.1.6
manufacturer's proposed recommended loading
m.r.l
amount of product in the test specimen corresponding to the mean loading which the manufacturer
recommends as necessary and achievable in practice in the analytical zone(s)
[EN 1001-2:2005, 4.50]

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EN 599-1:2009+A1:2013 (E)

3.1.7
maximum application limit

m.a.l
maximum amount in grams per square metre or kilograms per cubic metre of the product permitted for
application to the wood specimens in a particular biological test
[EN 1001-2:2005, 4.51]
3.1.8
mid-toxic value
m.t.v
arithmetic mean of the upper and lower toxic values as defined in the relevant European Standard biological
test
[EN 1001-2:2005, 4.52]
3.1.9
nominal effective retention
n.e.r
retention of test product calculated to give the efficacy specified in ENV 807 equivalent to the relevant target
retention of the reference preservative
[EN 1001-2:2005, 4.55]
3.1.10
penetrating treatment process
process which includes features or procedures intended to overcome the natural resistance of wood to
penetration by a wood preservative in its ready for use form
NOTE
Such processes include, for example, currently practised technologies of diffusion treatments, double-vacuum
and the vacuum/pressure process.

[EN 1001-2:2005, 4.58]
3.1.11
product
formulated wood preservative in the form as supplied for sale by the manufacturer
[EN 1001-2:2005, 1.32]
3.1.12

retention requirement
r.r
loading of product that is required in the analytical zone
NOTE
The retention requirement is expressed in grams of product per square metre for superficial application
processes and kilograms of product per cubic metre for penetrating treatment processes. It is derived from the critical
value in different ways depending upon the particular test involved.

[EN 1001-2:2005, 4.73]
3.1.13
superficial application process
process which does not include particular features or procedures intended to overcome the natural resistance
of wood to penetration of a wood preservative in its ready for use form
NOTE
Such processes include for example brush and spray techniques and short-time immersion (dipping)
processes in which wood normally has only a few minutes contact with the preservative.

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EN 599-1:2009+A1:2013 (E)

[EN 1001-2:2005, 4.82]
3.1.14
target retention
t.r
retention of reference preservative deemed to provide an adequate level of protection against biological attack
[EN 1001-2:2005, 4.87]
3.1.15

wood preservative
active ingredient(s) or preparations containing active ingredient(s) in the form in which they are placed on the
market, which are, on the basis of the properties of their active ingredient(s), intended either to prevent wooddestroying or wood-disfiguring organisms (fungi, insects and marine borers) from attacking wood and woodbased products, or to combat an attack by those organisms
[EN 1001-2:2005, 1.56]

3.2

Abbreviations and symbols

3.2.1
A
Anobium
3.2.2
B
Blue stain (see for example Table 2)
3.2.3
F
Field test (see for example Table 3a to Table 5)
3.2.4
H
Hylotrupes (see for example 5.2.3a) and Table 1)
3.2.5
I
All beetles (see for example 5.2.3c) and Table 1 to Table 5)
3.2.6
L
Lyctus (see for example 5.2.3a) and Table 1)
3.2.7
m/m
Mass/Mass (see for example 5.2.16 and 5.2.27)

3.2.8
N
Northern waters (see 5.2.28 and Table 5)
3.2.9
S
Southern waters (see 5.2.28 and Table 5)

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EN 599-1:2009+A1:2013 (E)

3.2.10
T
Termites (see for example 5.2.7 and Table 1 to Table 5)
3.2.11
V
Coriolus versicolor (see Table 3a and Table 3b)
3.2.12
n
V
nominal mean rating of replicates for non-destructive assessment in EN 330
3.2.13
e
V
nominal mean rating for external surfaces and those visible within the joint of replicates for destructive
assessment in EN 330
3.2.14
i

V
nominal mean rating for surfaces created by sawing of replicates for destructive assessment in EN 330
3.2.15
TP
R2
number of replicates treated with the wood preservative product with a rating of 2 or above in EN 330
3.2.16
R
R2
number of replicates treated with the reference preservative with a rating of 2 or above in EN 330
3.2.17
spp
species
3.2.18
unrP
upper nominal retention of the wood preservative product
3.2.19
lnrP
lower nominal retention of the wood preservative product
3.2.20
CAS number
Chemical Abstracts Service registry number
3.2.21
EINECS number
European Inventory of Existing Chemical Substances number
3.2.22
ELINCS number
European List of Notified Chemical Substances number

4

Assignment to use class(es)

Each product shall be assigned to one or more use class as described in EN 335-1.

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EN 599-1:2009+A1:2013 (E)

5
5.1

Efficacy
Principal requirements

5.1.1 Each product shall be tested in accordance with the tests specified in Table 1 to Table 5, taking into
consideration the following:
a)

relevant use class (see Clause 6);

b)

method of application (see Annex C);

c)

type of wood to which it is intended to be applied (see Annex D);

d)

which insect species (if any) it is to protect against;

e)

whether it is only be used under a coating.

Where Table 1 to Table 5 require the wood preservative product to be resistant to evaporative ageing and/or
leaching, tests in accordance with EN 73 and/or EN 84 shall be carried out separately (see Annex E).
5.1.2 Using the relevant criteria given in Table 1 to Table 5, the b.r.v. shall be established for each test at
an application rate at or below the given maximum application limit (see Annex G).
5.1.3 In those European standard biological tests which provide for the derivation of toxic values, the b.r.v.
shall be the mid toxic value (m.t.v.) except in the following cases:
a)

in tests with insects using EN 47, if the mortality at the lower toxic value is less than 80 %, then the upper
toxic value shall be used as the b.r.v;

b)

in tests with insects using EN 20-2 or EN 49-2, if the number of live larvae/emerged beetles at the lower
toxic value exceeds 20 % of the number from the solvent controls, then the upper toxic value shall be
used as the b.r.v;

c)

in EN 113 tests, if the mean mass loss at the lower toxic value is greater than 10 % (m/m), then the upper
toxic value shall be used as the b.r.v.

5.2

Additional requirements in Table 1 to Table 5

5.2.1 Where local conditions require the wood preservative product to have additional biological efficacy, or
to support the claims of manufacturers or the demands of specifiers, the product shall also be tested in
accordance with the relevant additional/local tests given in Table 1 to Table 5.
NOTE 1
Field tests which vary in form or geographical location from the procedures prescribed in EN 252, EN 275 and
EN 330 may provide relevant supplementary data for confirming retention requirements in EN 351 which are derived from
critical values according to EN 599.
NOTE 2

See Annex F.

5.2.2 The most relevant application process specified in the respective EN methods of biological test shall
be used even though this may not correspond precisely with the process intended for the test product in
practice.
5.2.3 Wood preservative products in use class 1 shall be effective against specific or all wood boring
beetles specified in this part of EN 599. There is no risk of fungal attack in this use class.
Tests of the product shall be carried out against one of the following:

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EN 599-1:2009+A1:2013 (E)

a)

unless b) or c) is applicable and is selected, all or each relevant individual beetle(s) “Hylotrupes bajulus
(H) “; “Anobium punctatum (A)”; and “Lyctus brunneus (L)”; or;

b)

the most tolerant beetle species, if the product is based on insecticide(s) for which data already exist
(aged or unaged) which show different activities against the different beetle species, that is the mid toxic
value of the least tolerant beetle species is less than 80 % of the mid toxic value of the most tolerant
beetle species; or;

c)

as defined in the column “for all beetle spp (I)”, if the product is based on insecticide(s) for which data
already exist (aged or unaged) which show more or less equal activity against all beetle species, that is
the mid toxic value of the least tolerant beetle species is equal to or greater than 80 % of the mid toxic
value of the most tolerant beetle species.

5.2.4 Additional tests need only be conducted when necessary to meet or confirm local or regional
requirements, the stated requirements of specifiers or the specific claims of manufacturers (see 5.2.1).
5.2.5

The product shall be tested according to EN 46-2 if a repellent or ovicidal activity is claimed.

5.2.6 Leaching in accordance with EN 84 is not required for products intended for use classes 1
and 2 which shall be protected from prolonged exposure to rain or other accidental and temporary forms of
wetting during transport and installation. If such protection cannot be ensured, products intended for use in
use classes 1 and 2 to be applied by methods which will not achieve lateral penetrations greater than 3 mm,
shall be tested after leaching to EN 84.
Leaching according to EN 84 cannot be used in conjunction with EN 20-1 and EN 20-2; therefore the b.r.v.

against Lyctus brunneus (L) where there is a leaching hazard shall be deemed to be equal to that for:
Hylotrupes bajulus (H) or
Anobium punctatum (A) after leaching; whichever is the greater.
5.2.7 For the anti-termite qualification “T”, a superficial application product shall have been tested in ready
for use form:
a)

according to EN 46 or the relevant beetle test and have achieved the criteria to provide the biological
reference value for this test and;

b)

according to EN 118:2005 when applied at 200 g/m of treating product and have achieved the efficacy
criteria defined for termites in this part of EN 599.

2

NOTE
European Standard EN 118:2005 does not provide an adequate test of all the modes of action of currently
available preservative products. By the time a new version of EN 118 or an alternative European test standard providing
more realistic test results for efficacy against termites is produced, other applicable evaluation methods may be used to
provide the b.r.v.

5.2.8 EN 20-2 shall not be used for water-dispersed or water-soluble types of wood preservative products
because the scope of the method does not permit its use with such products.
5.2.9 When deriving the mid toxic value, if the mortality at the lower toxic value in EN 47 is less than 80 %,
the upper toxic value shall be used as the b.r.v. In EN 20-2 and EN 49-2, if the number of live larvae/emerged
beetles at the lower toxic value exceeds 20 % of the number from the solvent controls, the upper toxic value
shall be used as the b.r.v.
5.2.10 The required retention in the respective laboratory tests shall not exceed the manufacturers

recommended loading and shall be achieved for the laboratory tests by full impregnation with a diluted
solution of the product.

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EN 599-1:2009+A1:2013 (E)

NOTE
In industrial practice, products intended for penetrating treatment processes are applied in ways which result
in a wide range of retentions of the product in treated sapwood. For example, in Scots pine (European redwood) (Pinus
sylvestris) sapwood, high pressure systems (for example vacuum-pressure) would normally be expected to achieve close
3
to mean retentions of 600 kg/m of treating product. Low and non-pressure systems (for example double-vacuum,
3
prolonged immersion) would not normally be expected to achieve mean retentions of more than 300 kg/m of treating
product.

5.2.11 EN 113 tests shall be used as the specified test against basidiomycetes for products intended for use
in use class 2 applied by superficial application.
5.2.12 CEN/TS 839 is not a required test for use class 2. However, where products have been tested in
accordance with CEN/TS 839 for use class 3 (uncoated), these products may also be used in use class 2
without further testing in accordance with EN 113. In this case, the b.r.v. for protection against basidiomycetes
in use class 2 may be determined from CEN/TS 839 plus ageing according to EN 73, using the criteria
described in 5.2.19, and where relevant EN 84 as described in 5.2.6.
5.2.13 EN 113 tests with Coriolus versicolor are not required for products intended only for use class 2.
5.2.14 Products for use in use class 2 intended to be effective against fungi causing blue stain in service
shall be tested in accordance with EN 152, but after ageing in accordance with EN 73, or after natural or
artificial weathering as given in EN 152.

5.2.15 In order to derive the critical value, the biological reference value in grams per square metre shall be
deemed to be equivalent to twice the biological reference value established in kilograms per cubic metre in the
EN 113 test.
5.2.16 When deriving the mid toxic value, the mean loss in mass of the samples at the lower toxic value in
the EN 113 test shall not exceed 10 % (m/m); if this value is exceeded then the upper toxic value shall be
taken as the biological reference value.
5.2.17 Products proposed for superficial treatments and intended for use class 3 without any paint or coating
shall be tested in accordance with CEN/TS 839 after EN 73 and EN 84 separately.
5.2.18 Products proposed for superficial treatment and intended for use class 3 under a paint or other
coating applied before exposure in use shall be tested either according to CEN/TS 839 after EN 73 and EN 84
separately or in accordance with EN 113 after EN 73 and EN 84 separately, or according to EN 330 as well as
with EN 113 after EN 73.
5.2.19 Products intended for use class 3 shall be tested according to the application options in accordance
with either EN 113 or CEN/TS 839 using Coriolus versicolor, only if this is required as an additional biological
test; if used, efficacy shall be demonstrated either in Scots pine (Pinus sylvestris) or in beech (Fagus
sylvatica), or in both, depending whether efficacy is claimed or specified for softwoods, for hardwoods, or for
both.
NOTE
EN 113 or CEN/TS 839 tests with Coriolus versicolor need not be conducted in both types of wood providing
there is evidence that the active ingredient(s) in the product are equally effective in both timbers; in this case the b.r.v. for
the product in beech can be used for both timbers. If the evidence shows the active ingredient(s) to be more effective in
Scots pine, then the b.r.v. for the product for use on softwoods should be derived from tests in Scots pine to avoid over
specification of biocides.

5.2.20 For the purposes of deriving critical values from CEN/TS 839, the biological reference value shall be
the lowest application level (in grams per square metre) at which the mean mass loss is less than 3 %,
providing that:
a)

no more than one of the test specimens at that level (exposed to any particular ageing procedure and any

particular test fungus), shows internal decay; or

13


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

b)

of the specimens which show signs of decay exclusively at their surface, no more than one has a mass
loss greater than 3 % but less than 5 %, irrespective of the number of valid replicates.

5.2.21 The biological reference value shall be the amount of the product at which the values for the nominal
n
e
mean rating for decay (V ) and the mean decay ratings for external surfaces and joint faces (V ) and the mean
i
decay ratings for internal conditions (V ) are equal to or better than those for the reference preservative after
the untreated control specimens have reached a median rating of ≥ 3; in addition the number of replicates
TP
rated 2 or above for the product (R2 ) shall not exceed the number rated 2 or above for the reference
R
preservative (R2 ).
5.2.22 Products proposed for penetrating treatments and intended for use class 3 without any paint or
coating shall be tested in accordance with EN 113 after EN 73 and EN 84 separately.
5.2.23 Products proposed for penetrating treatments and intended for use class 3 under a paint or other
coating applied before exposure in use shall be tested either according to EN 113 after EN 73 and EN 84
separately or in accordance with EN 330 as well as with EN 113 after EN 73.
5.2.24 Products intended for use in use classes 4 and 5 shall be tested in accordance with EN 113 using

Coriolus versicolor and for this fungus, efficacy shall be demonstrated either in Scots pine or in beech, or in
both, depending whether efficacy is claimed or specified for softwoods, for hardwoods, or for both.
NOTE
EN 113 tests with C. versicolor need not be conducted in both types of wood providing there is evidence that
the active ingredient(s) in the product are equally effective in both timbers; in this case the b.r.v. for the product in beech
can be used for both timbers. If the evidence shows the active ingredient(s) to be more effective in Scots pine, then the
b.r.v. for the product for use on softwoods should be derived from tests in Scots pine to avoid over specification of
pesticide.

5.2.25 Products intended for use in use class 4 shall be tested in accordance with EN 252 only if required as
an additional biological test. The relevant code for conformance with field test requirements (see EN 599-2)
shall be used only if the biological reference value for tests in accordance with EN 252 has been taken into
account before deriving the critical value.
5.2.26 Tests in accordance with ENV 807 shall be carried out and the nominal effective retention calculated
in accordance with Clause 10 and Annex E of ENV 807:2001. Data derived from the screening test method
described in Annex A of ENV 807:2001 are not admissible.
NOTE
The b.r.v. can be derived also from exposure periods less than 32 weeks if for the exposure period of
24 weeks no mean mass loss value below 3,0 % is reported for the reference product, but, values suitable for deriving the
b.r.v. are available for lower exposure periods (e.g. for 16 and 24 weeks exposure). In this case the highest concentration
of the reference product should be used for the calculation which shows a mean mass loss < 3,0 % at the shorter
exposure time and a mass loss of > 3,0 % at the longer exposure time.

5.2.27 The biological reference values for EN 252 and EN 275 shall be determined separately for each of
two or more different field test sites in each case. The test sites shall have different soil/marine characteristics
and be located in different geographical regions giving different climatic conditions; at least one site shall be
within geographic Europe.
In the case of EN 275, the reference specimens to be included in the test method shall be treated with either:
a)

the reference preservative at concentrations of 2,6 % (m/m) and 0,6 % (m/m), to provide two sets of
3
3
specimens with retentions of approximately 18 kg/m and 4 kg/m respectively; or

b)

the alternative reference preservative at 5 % (m/m) and 1,2 % (m/m) to provide two sets of specimens
3
3
with retentions of approximately 30 kg/m and 7 kg/m respectively.

The biological reference value for the product shall be derived by plotting the rating against the retention of
preservative for both the reference preservative and the product under test for each site. The upper nominal
retention of the product is calculated as that retention which has a rating equal to the rating of the higher

14


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

retention of the reference preservative. The lower nominal retention of the product is calculated as that
retention which has a rating equal to the rating of the lower retention of the reference preservative.
If variability in the test results yields more than a single upper or lower nominal retention value for the product,
in any given test site the mean of all the upper or lower values shall be used for deriving the biological
reference values.
If after five years there is no biological attack at the upper retention of the reference preservative, the test shall
be continued until attack occurs in these samples.
If after five years the mean rating for the biological attack in the upper retention of the reference preservative

is less than 0,5, then the biological reference value for the product shall be derived from both the upper and
lower nominal retentions of the product.
If after five years the mean rating for the upper retention of the reference preservative is equal to or greater
than 0,5, then the biological reference value for the product shall be derived from the upper nominal retention
of the product only.
The upper biological test value shall be equal to the upper nominal retention of product divided by 0,75 and
the lower biological test value shall be equal to the lower nominal retention of product divided by 0,17. The
biological reference value for deriving the critical value shall be the higher of the two nominal mean values
calculated from the upper and lower biological test values at each site.
If the product is claimed to be effective against termites (T) one of the test sites shall be recognised as having
active termites.
5.2.28 Northern waters (N) are those with temperatures normally ranging from below 0 °C to 20 °C (north of
latitude 50°N); southern waters (S) are those with temperatures normally ranging from 10 °C to 30 °C (south
of latitude 45°N).
If specific test data for a product exist only for either northern or southern waters of the geographic European
area respectively, the biological reference value from one can be derived from tests conducted in the other by
application of the following factors:
b.r.v. for southern waters = 1,5 * b.r.v. derived in northern waters.
b.r.v. for northern waters = 0,67 * b.r.v. derived in southern waters.

6
6.1

Tests for efficacy according to use class
Use class 1

Wood preservative products intended for use by superficial or penetrating application processes in use class 1
shall demonstrate efficacy in accordance with Table 1 against one of the following:
a)

those individual beetle species against which activity is required or is to be claimed; or
for products based on insecticide(s) for which there are existing data from tests to European standard
methods which show markedly different activities against the different individual beetles (see requirement
5.2.3 b));

b)

the most tolerant beetle species for that insecticide;

15


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EN 599-1:2009+A1:2013 (E)

or for products based on insecticide(s) for which there are existing data from tests to European standard
methods to confirm that activity is more or less equal against each of the specific individual beetles (see
requirement 5.2.3 c));
c)

Hylotrupes bajulus as a representative beetle species.
Tests after leaching in accordance with EN 84 are not required unless effective protection from prolonged
exposure to rain or other accidental and temporary forms of wetting during transport and installation is in
doubt in which case requirement 5.2.6 applies.

6.2

Use class 2

Wood preservative products intended for use by superficial or penetrating application processes in use class 2

shall demonstrate efficacy in accordance with Table 2 against wood destroying basidiomycetes, excluding
Coriolus versicolor, after ageing in accordance with EN 73.
CEN/TS 839 is not a required test for use class 2. However, where products have been tested in accordance
with CEN/TS 839 for use class 3 (uncoated), these products may also be used in use class 2 without further
testing in accordance with EN 113. In this case, the b.r.v. for protection against basidiomycetes in use class 2
may be determined from CEN/TS 839 plus ageing according to EN 73, using the criteria described in 5.2.19.
Additionally, for all products if insecticidal activity is required or to be claimed they shall demonstrate efficacy
against the relevant biological agents using the appropriate tests listed for use class 1 above.
Tests after leaching in accordance with EN 84 are not required unless effective protection from prolonged
exposure to rain or other accidental and temporary forms of wetting during transport and installation is in
doubt in which case requirement 5.2.6 applies.
Where additional biological efficacy is to be claimed or required against fungi causing blue stain in service, the
product shall demonstrate efficacy in accordance with EN 152, Table 2, but after ageing in accordance
with EN 73, or after natural or artificial weathering as given in EN 152.
Where additional biological efficacy is claimed or required against beetles or fungi the product shall
demonstrate efficacy in accordance with the relevant insect tests in Table 1 after evaporative ageing in
accordance with EN 73.

6.3

Use class 3

Wood preservative products intended for use classes 3.1 and 3.2 in accordance with EN 335-1 shall
demonstrate efficacy in accordance with Table 3a for superficial applications or Table 3b for penetrating
processes.
Wood preservative products intended for superficial application processes in use class 3 without further
protection from a coating shall demonstrate efficacy in accordance with Table 3a against wood destroying
basidiomycete fungi in accordance with CEN/TS 839 after EN 73 and after EN 84 separately but excluding
Coriolus versicolor, unless requirement 5.2.19 applies.
Wood preservative products intended for penetrating application processes in use class 3 without further

protection from a coating shall demonstrate efficacy in accordance with Table 3b against wood destroying
basidiomycete fungi in accordance with EN 113 after EN 73 and after EN 84 separately but excluding Coriolus
versicolor, unless requirement 5.2.19 applies.
Wood preservative products intended for penetrating application processes in use class 3, and intended for
use in conjunction with a paint or other coating, or with some other form of protective covering applied prior to
exposure which protects the preservative from leaching, shall demonstrate efficacy in accordance with
Table 3b against either of the following two options:

16


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

a)

wood destroying basidiomycete fungi in accordance with EN 113 after EN 73 and after EN 84 separately
but excluding Coriolus versicolor, unless requirement 5.2.19 applies; or

b)

wood destroying basidiomycete fungi in accordance with EN 113 after EN 73 only but excluding Coriolus
versicolor, unless requirement 5.2.19 applies, and the product shall satisfy the efficacy criteria given in
Table 3 for EN 330 after the untreated control has reached a median rating of 3;

Wood preservative products intended for superficial application processes in use class 3, and intended for use
in conjunction with a paint or other coating, or with some other form of protective covering applied prior to
exposure which protects the preservative from leaching, shall demonstrate efficacy in accordance with
Table 3a against either of the two options a) and b) above or:
c)

wood destroying basidiomycete fungi in accordance with CEN/TS 839 after EN 73 and after EN 84
separately but excluding Coriolus versicolor, unless requirement 5.2.19 applies.

In the case of products tested according to EN 113 after EN 73 and EN 330, the product information shall
include the statement: Components treated with this product shall be protected from the weather until after the
top coating has been applied.
In the case of products tested according to either EN 113 or CEN/TS 839, both after EN 73 and EN 84
separately, the product information shall include the statement: Components treated with this product should
receive any top coating as soon as practically convenient after manufacture but in the meantime, no special
protection from the weather is required.
Additionally, for those products for which insecticidal activity is required or to be claimed, the product shall
demonstrate efficacy against the relevant biological agents using the appropriate tests listed in Table 1 for use
class 1 above but also after a separate EN 84 leaching test unless the product is intended for use under a
coating (see requirement 5.2.6).
Where additional biological efficacy is to be claimed or required either in field tests, against Coriolus versicolor
in laboratory tests or against fungi causing blue stain in service, the product shall demonstrate efficacy in
accordance with the provisions of EN 330, EN 113 (including Coriolus versicolor), CEN/TS 839 (including
Coriolus versicolor) and/or EN 152 respectively as given in Table 3a for superficial applications or Table 3b for
penetrating processes.

6.4

Use class 4

Wood preservative products intended for both use classes 4.1 and 4.2 shall demonstrate efficacy in
accordance with Table 4 against:
a)

wood destroying basidiomycete fungi in accordance with EN 113 after EN 73 and after EN 84 separately

and including Coriolus versicolor in beech and/or Scots pine; and

b)

soft rotting micro-fungi in accordance with ENV 807.

Where the b.r.v. from additional tests in accordance with EN 252 is lower than any b.r.v. derived from
ENV 807, that from the EN 252 test may be used to take precedence over that from ENV 807 in deriving the
critical value for the test preservative, provided that there is evidence of active soft rot in the stakes treated at
the lower retention of the reference preservative at the time of evaluation. For this to apply it must be
established that decay in the set of reference stakes is not attributable to the presence of basidiomycete fungi.
The b.r.v. from EN 252 shall not take precedence over a higher value derived from tests in accordance with
EN 113.
Additionally, for those products for which insecticidal activity is required or to be claimed, the product shall
demonstrate efficacy against the relevant biological agents using the appropriate tests listed for use class 1
above but also after a separate EN 84 leaching test (see requirement 5.2.6).

17


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Where additional biological efficacy is to be claimed or required in field tests or against fungi causing blue
stain in service, the product shall demonstrate efficacy in accordance with the requirements defined for the
tests according to EN 252 or EN 152as appropriate, given in Table 4.

6.5

Use class 5

Wood preservative products intended for use class 5 shall demonstrate efficacy according to the criteria in
Table 5 against:
a)

wood destroying basidiomycete fungi in accordance with EN 113 after EN 73 and after EN 84 separately
and including Coriolus versicolor in beech and/or Scots pine, and;

b)

soft rotting micro-fungi in accordance with ENV 807, and;

c)

marine organisms in accordance with EN 275 after a minimum period of five years.

NOTE
Where the b.r.v. from tests in accordance with EN 275 is lower than any b.r.v. derived from ENV 807, that
from EN 275 may be used to take precedence in deriving the critical value for the test preservative.

Additionally, for those products for which insecticidal activity is required or to be claimed, the product shall
demonstrate efficacy against the relevant biological agents using the appropriate tests listed for use class 1
above but also after a separate EN 84 leaching test (see requirement 5.2.6).
Where additional biological efficacy is to be claimed or required against fungi causing blue stain in service, the
product shall demonstrate efficacy in accordance with the requirements defined for the tests according to
EN 152, given in Table 5.

7

Derivation of critical values

The biological reference value is derived from the results of each specific biological test and shall be equal to
or lower than the maximum application limit.
The critical value is the highest biological reference value obtained from all the tests carried out in accordance
with those listed for a given use class in this European Standard. Where additional claims are made for a
product the b.r.v.(s) from any relevant additional test(s) shall be taken into account when determining the
critical value.
NOTE 1

See Annex G.

The critical value for products to be applied by superficial application processes shall be expressed as grams
per square metre.
The critical value for products to be applied by penetrating treatment processes shall be expressed as
kilograms per cubic metre.
Critical values derived from dated European Standards methods of test which are subsequently revised shall
remain valid unless the revised standard method states to the contrary (see Annex H).
NOTE 2
For product variants based on a specific single formulation having data to the requirements of Table 1 to
Table 5 in this part of EN 599, critical values may be declared on the basis of no new biological testing, minimum new
biological testing or full new biological testing in accordance with Annex A.

18


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

8

Marking

Information on the biological efficacy of the preservative product in relation to the use classes intended by the
producer shall be provided in accompanying documentation in accordance with EN 599-2.

9

Product identification

9.1

Type testing

A sample representative of the product shall be tested in accordance with those methods specified as the
minimum for each use class (together with any additional tests) for which protection is claimed.
Reports of the tests conducted shall be kept by the producer of the wood preservative product, together with a
copy of the declaration of the identity of the product supplied with the sample submitted for testing, as long as
the product is on the market, and for a period of five years after the product is withdrawn from the market.

9.2

Identity of the preservative product

A written declaration of the identity of the product shall be produced to accompany the sample. It shall state:
a)

the name or other designation of the preservative product to be tested;

b)

the composition of the preservative product to be tested. This shall include where available the chemical
designation, the CAS, EINECS or ELINCS numbers and the trade or common name of the active
ingredient(s) together with a generic description of the co-formulants (for example co-solvent, resin) in the
preservative product, and the composition in terms of per cent mass/mass of all these ingredients. The
sum of the percentages shall equal 100 %. Safety information shall be provided;

Other than for the active ingredients, the supplier is permitted to declare the ingredients under a confidentiality
agreement between the parties. In such circumstances the composition, other than active ingredient(s), need
be declared in the test report in generic terms only.
c)

the result of an analysis of the actual sample submitted for test. This shall in every case include analysis
for active ingredients. If analysis is carried out under a quality system this shall be stated in the
declaration.

NOTE

Analyses should be carried out preferably under a quality system such as EN ISO/IEC 17025.

The declaration shall be signed by the supplier or his authorized representative.

19


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Table 1 — Efficacy criteria in biological tests for use class 1
Use
class

mark

Proposed
type of
commercial
application
(5.2.2)

Requirements

Superficial
application

either

20

Additional Test
(5.2.4)

or

Hylotrupes bajulus
(H)

Anobium punctatum
(A)

Lyctus brunneus (L)

for all beetle spp (I)

Termites (T)

Test

EN 46–1
or EN 46–2
(5.2.6)

EN 49–1

EN 20–1

EN 46–1 or EN 46–2
or (A) or (L) (see 5.2.3
b + 5.2.3 c and 5.2.5)

EN 118

Ageing

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

Maximum application
limit in test

200 g/m
(dip or pipette)

200 g/m
(dip or pipette)

200 g/m
(dip or pipette)

200 g/m
(dip or pipette)

200 g/m
(dip or pip.) (5.2.7)

Criterion for biological
reference value

EN 46–1 100 %
mortality at end of test,

EN 46–2 100 %
repellence (no eggs
deposited at treated
specimens although
fertility of the beetles
was proved) or 100 %
total mortality at the
end of test

no live larvae at end of
test

no live larvae/emerged
beetles at end of test

EN 46–1 100 %
mortality at end of
test, EN 46–2 100 %
repellence (no eggs
deposited at treated
specimens although
fertility of the beetles
was proved) or 100 %
total mortality at the
end of test

no damage of
rating > 2, only one
sample 2

Test

EN 47
(5.2.9)

EN 49–2
(5.2.9)

EN 20–2 (5.2.8)
(5.2.9)

EN 47 or (A) or (L)
(see 5.2.3 b + 5.2.3 c)

EN 117

Ageing

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

in accordance with
EN 73 (5.2.6)

Maximum application
limit in test

m.r.l. for Scots pine
sapwood (5.2.10)

m.r.l. for Scots pine
sapwood (5.2.10)

m.r.l. for oak

m.r.l. for Scots pine
sapwood (5.2.10)

m.r.l. for Scots pine
sapwood (5.2.10)

Criterion for biological
reference value

m.t.v. (5.2.3)

m.t.v. (5.2.3)

m.t.v. (5.2.3)

m.t.v. (5.2.3)

No damage of
rating > 2, only one
sample 2

1

Penetrating
process

Minimum requirements for beetle spp tests (5.2.3)

2

2

2

2

2


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Table 2 — Efficacy criteria in biological tests for use class 2
Use
class
mark

Proposed
type of
commercial
application
(5.2.2)

Requirement

Superficial
application

Minimum requirements for
fungal test
Basidiomycetes (5.2.2)

Blue stain (B)

Beetle spp (5.2.3) (I)

Termites (T)

Test

EN 113 (5.2.11 & 5.2.15, 5.2.12)
(not C. versicolor) (5.2.13)

EN 152

If required add from

use class 1

If required add from
use class 1

Ageing

in accordance with EN 73 (5.2.6)

In accordance with EN 73
(5.2.14)

Maximum application
limit in test

100 kg/m
(5.2.15)

Criterion for biological
reference value

m.t.v. most tolerant fungus (5.2.16)

at end of test no individual
rating ≥ 2; min. stain-free zone
1,0 mm, mean ≥ 1,5 mm

Test

EN 113 (not C. versicolor) (5.2.13)

EN 152

If required add from
use class 1

If required add from
use class 1

Ageing

in accordance with EN 73 (5.2.6)

in accordance with EN 73 (5.2.14)

Maximum application
limit in test

m.r.l. for Scots pine (5.2.10)

as specified by manufacturer for
commercial use

Criterion for biological
reference value

m.t.v. most tolerant fungus (5.2.16)

at end of test no individual
rating ≥ 2; min. stain-free zone

1,0 mm, mean ≥ 1,5 mm

3

2
Penetrating
process

Additional / local tests (5.2.4)

200 g/m

2

21


BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Table 3a — Efficacy criteria in biological tests for use class 3 (superficial application)
Minimum requirements for fungal test of preservatives for
use
Proposed
Use
type of
class commercial Requirements
mark application
(5.2.2)

Test

3

Superficial
application

Ageing

Maximum
application limit in
test

22

with or
without
coatings

only under coatings

Additional / local tests (5.2.4)

Field test
(F)

Coriolus
versicolor (V)

Blue stain

(B)

Option 1
(5.2.17)

Option 2 (5.2.18)

Basidiomycetes

Basidiomycetes

Basidiomycetes

Field tests

CEN/TS 839
(not C.
versicolor)
(5.2.19)

EN 113 (5.2.15)
(not C. versicolor)
(5.2.19)

EN 113 (5.2.15)
(not C.
versicolor)
(5.2.19)

EN 330

EN 330

CEN/TS 839 or
EN 113 (in
Scots pine
sapwood and /
or beech)
(5.2.19)

EN 152

in accordance
with EN 73
(5.2.18)

no additional
ageing
according to
laboratory
methods

no additional
ageing
according to
laboratory
methods

in accordance
with EN 73 and

EN 84
separately
(5.2.17)

as given in
EN 152

m.r.l.

m.r.l.

100 kg/m
(5.2.15)

in accordance
in accordance
with EN 73 and
with EN 73 and
EN 84 separately EN 84 separately
(5.2.17)
(5.2.18)
200 g/m

2

100 kg/m
(5.2.15)

3

Option 3 (5.2.18)

100 kg/m
(5.2.15)

3

3

200 g/m

2

Beetle
spp (I)
(5.2.3)

If required
add from
use
class 1+
EN 84

Termites
(T)

If required
add from
use class 1
+ EN 84

BS EN 599-1:2009+A1:2013
EN 599-1:2009+A1:2013 (E)

Superficial
application

3

Criterion for
biological
reference value

lowest application
level at which no
more than one of
the test specimens
shows visible
decay inside the
specimens and not
more than one
specimen showing
signs of decay
exclusively at its
surface has
suffered a loss in
mass greater than
3,0 % (m/m) but
less than 5,0 %

(m/m) independent
of the number of
valid replicates.
(5.2.20)

m.t.v. most
tolerant fungus
(5.2.16)

m.t.v. most
tolerant fungus
(5.2.16)

after the
after the
untreated
untreated
control
control
specimens
specimens
have
have
reached a
reached a
median
median
rating of ≥ 3: rating of ≥ 3:
n
e

I
n
e
I
V , V and V V , V and V
equal or
equal or
better than
better than
for reference for reference
TP
TP
and R2
and R2
equal or less equal or less
R
R
than R2 .
than R2 .
(5.2.21)
(5.2.21)

at end of test
for EN 113:
m.t.v. (5.2.16).
no individual
rating ≥ 2; min.
For
CEN/TS 839: stain-free zone
1,0 mm,

lowest
mean ≥ 1,5 mm
application
level at which
no more than
one of the test
specimens
shows visible
decay inside
the specimens
and not more
than one
specimen
showing signs
of decay
exclusively at
its surface has
suffered a loss
in mass
greater than
3,0 % (m/m)
but less than
5,0 % (m/m)
independent of
the number of
valid replicates
(5.2.20)

23