Bsi bs en 61300 2 16 2006

Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

BRITISH STANDARD

Fibre optic
interconnecting
devices and passive
components — Basic
test and measurement
procedures —
Part 2-16: Tests — Mould growth

The European Standard EN 61300-2-16:2006 has the status of a
British Standard

ICS 33.180.20

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BS EN
61300-2-16:
2006


BS EN 61300-2-16:2006

Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

National foreword
This British Standard was published by BSI. It is the UK implementation of
EN 61300-2-16:2006. It is identical with IEC 61300-2-16:2006. It supersedes

BS EN 61300-2-16:1997 which is withdrawn.
The UK participation in its preparation was entrusted by Technical Committee
GEL/86, Fibre optics, to Subcommittee GEL/86/2, Fibre optic interconnecting
devices and passive components.
A list of organizations represented on GEL/86/2 can be obtained on request to
its secretary.
This publication does not purport to include all the necessary provisions of a
contract. Users are responsible for its correct application.
Compliance with a British Standard cannot confer immunity from
legal obligations.

This British Standard was
published under the authority
of the Standards Policy and
Strategy Committee
on 31 January 2007

© BSI 2007

ISBN 978 0 580 50039 8

Amendments issued since publication
Amd. No.

Date

Comments


EUROPEAN STANDARD

EN 61300-2-16

Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

NORME EUROPÉENNE
November 2006

EUROPÄISCHE NORM
ICS 33.180.20

Supersedes EN 61300-2-16:1997

English version

Fibre optic interconnecting devices and passive components Basic test and measurement procedures
Part 2-16: Tests Mould growth
(IEC 61300-2-16:2006)
Dispositifs d'interconnexion
et composants passifs à fibres optiques Méthodes fondamentales d'essais
et de mesures
Partie 2-16: Essais Moisissures
(CEI 61300-2-16:2006)

Lichtwellenleiter Verbindungselemente
und passive Bauteile Grundlegende Prüf- und Meßverfahren
Teil 2-16: Prüfungen Schimmelwachstum
(IEC 61300-2-16:2006)

This European Standard was approved by CENELEC on 2006-10-01. CENELEC members are bound to comply

with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard
the status of a national standard without any alteration.
Up-to-date lists and bibliographical references concerning such national standards may be obtained on
application to the Central Secretariat or to any CENELEC member.
This European Standard exists in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CENELEC member into its own language and notified
to the Central Secretariat has the same status as the official versions.
CENELEC members are the national electrotechnical committees of Austria, Belgium, Cyprus, the Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, the Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.

CENELEC
European Committee for Electrotechnical Standardization
Comité Européen de Normalisation Electrotechnique
Europäisches Komitee für Elektrotechnische Normung
Central Secretariat: rue de Stassart 35, B - 1050 Brussels
© 2006 CENELEC -

All rights of exploitation in any form and by any means reserved worldwide for CENELEC members.
Ref. No. EN 61300-2-16:2006 E


EN 61300-2-16:2006

–2–

Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

Foreword

The text of document 86B/2345/FDIS, future edition 2 of IEC 61300-2-16, prepared by SC 86B, Fibre
optic interconnecting devices and passive components, of IEC TC 86, Fibre optics, was submitted to
the IEC-CENELEC parallel vote and was approved by CENELEC as EN 61300-2-16 on 2006-10-01.
This European Standard supersedes EN 61300-2-16:1997.
The specific technical changes from EN 61300-2-16:1997 are as follows:
– Single Variant, Variant 1, was introduced instead of two variants, Variant 1 and 2.
– Deterioration tests were clarified.
– Detailed test procedures were introduced.
– Severity was clarified.
The following dates were fixed:
– latest date by which the EN has to be implemented
at national level by publication of an identical
national standard or by endorsement

(dop)

2007-07-01

– latest date by which the national standards conflicting
with the EN have to be withdrawn

(dow)

2009-10-01

Annex ZA has been added by CENELEC.
__________

Endorsement notice
The text of the International Standard IEC 61300-2-16:2006 was approved by CENELEC as a

European Standard without any modification.
__________


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–3–

EN 61300-2-16:2006

CONTENTS
1

Scope ............................................................................................................................4

2

Normative references .....................................................................................................4

3

Abbreviations .................................................................................................................4

4

General description ........................................................................................................4

5

4.1 Health hazards to operators...................................................................................5

4.2 Microbial strains ....................................................................................................5
Procedure ......................................................................................................................5

6

5.1 General .................................................................................................................5
5.2 Test samples .........................................................................................................6
5.3 Test ......................................................................................................................6
Severity..........................................................................................................................6

7

Details to be specified ....................................................................................................6

Bibliography .........................................................................................................................7
Annex ZA (normative) Normative references to international publications with their
corresponding European publications .............................................................................8
Table 1 – Test fungi ..............................................................................................................5


Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

EN 61300-2-16:2006

–4–

FIBRE OPTIC INTERCONNECTING DEVICES
AND PASSIVE COMPONENTS –
BASIC TEST AND MEASUREMENT PROCEDURES –
Part 2-16: Tests – Mould growth

1

Scope

This part of IEC 61300, when required by the relevant specification, evaluates the ability of
the materials used for passive fibre optic devices to withstand the action of fungi and bacteria
and soil microorganisms likely to be encountered during usage. The type and extent of
material deterioration may be determined by visual examination and/or changes in mass or
any other physical property.
Since mould growth conditions include high relative humidity, the test is applicable to passive
optic devices under humid operating conditions according to IEC 61753-1, in storage and/or
transport.

2

Normative references

The following referenced documents are indispensable for the application of this document.
For dated references, only the edition cited applies. For undated references, the latest edition
of the referenced document (including any amendments) applies.
IEC 60068-2-10: Environmental testing – Part 2-10: Tests – Test J and guidance: Mould
growth
IEC 61300-1: Fibre optic interconnecting devices and passive components – Basic test and
measurement procedures – Part 1: General and guidance
IEC 61753-1: Fibre optic interconnecting devices and passive components – Part 1: General
and guidance for performance standard

3

Abbreviations

For the purposes of this document, the following abbreviations apply.
ATCC: American Type Culture Collection (www.atcc.org)

4

General description

This procedure is conducted in accordance with IEC 60068-2-10, test J. This test covers the
inoculation of fibre optic products with a selection of mould spores followed by a period of
incubation under conditions which promote spore germination and the growth of mould.


EN 61300-2-16:2006

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–5–

Two variations of the test are given. Variant 1 specifies inoculation of the specimen with the
mould spores without nutrients, whereas Variant 2 specifies the inoculation with the mould
spores suspended in a nutritive solution which supports mould growth. For the environmental
categories described in IEC 61753-1 Variant 1 shall be applied.
The deterioration of the specimen may result in swelling that would destroy functional utility,
cause loss of physical strength, and cause changes in other important mechanical properties.
Therefore the following deterioration tests shall be done:
a) visual examination;
b) change of mass and dimensions;
c) changes in other physical properties, i.e. material strength characteristics.

It is advisable to use testing procedures such as specified for plastics in ISO 846 to assess
the vulnerability to damage by mould growth of the constructional materials used.
4.1

Health hazards to operators

The mould growth test can constitute a health hazard, unless special precautions according
to IEC 60068-2-10 are taken.
4.2

Microbial strains

The fungi identified in Table 1 shall be used for performing the test and preparing the
cultures.
Table 1 – Test fungi
No.

Name

Strain No.

Attacks

1

Aspergillus niger

ATCC 9642

Grows profusely on many materials and

is resistant to copper salts

2

Aureobasidium pullulans

ATCC 15233

Attacks paints and lacquers

3

Penicillium funicolosum/pinophilum

ATCC 11797

Attacks many materials especially
textiles

4

Chaetomium globosum

ATCC 6205

-

5

Gliocladium virens

ATCC 9645

-

(For purchase of a test fungus, access www.astm.org.)

5
5.1

Procedure
General

Preparation and assessment of the specimens shall be done at the standard conditions
according to IEC 61300-1.
The conditions of incubation are (29 ± 1) °C and a relative humidity of >90 % and <100 %.


EN 61300-2-16:2006

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5.2

–6–

Test samples

Test samples shall be representative of production components/completed product.
For each test sample and method, 3 batches of specimens shall be prepared:

– control specimen stored under standard atmospheric conditions according to IEC 61300-1;
–

specimens inoculated and incubated;

–

sterile specimens incubated as batch 2.

For visual examination and mass and/or other physical changes determination, at least
3 specimens for each batch shall be prepared, i.e. 9 specimens each per sample and per test
method.
Do not clean and label the specimen. Labelling shall be done for example by use of
containers.
5.3

Test

Test specimens are exposed to test Variant 1 according to IEC 60068-2-10.
At the end of the exposure the specimens are inspected before or after cleaning according to
visual examination and/or changes in mass or other physical properties.
The results are obtained by comparison of the specimens exposed to the biological attacks
(batch 1) and those stored without contamination under standard (batch 2) and test (batch 3)
environmental conditions.

6

Severity

The specimen shall be incubated at the chosen environmental conditions for 28 days, or

longer by agreement between the customer and supplier.

7

Details to be specified

The following details shall be specified:
–

specimen configuration;

–

all information needed for identification of the specimen/product;

–

the fungi, bacteria and soil used;

–

the type of the microbicidal solution;

–

the physical properties measured;

–

the test methods used to measure physical properties;

–

initial examinations and measurements and performance requirements;

–

final examinations and measurements and performance requirements;

–

deviations from test procedure;

–

additional pass/fail criteria.


–7–

EN 61300-2-16:2006

Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

Bibliography
ASTM G 21: Standard Practice for Determining Resistance of Synthetic Polymeric Materials
to Fungi (for purchase of the standard, access www.astm.org)
ISO 846: Plastics – Evaluation of the action of microorganisms
___________

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EN 61300-2-16:2006

–8–

Annex ZA
(normative)
Normative references to international publications
with their corresponding European publications
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
NOTE When an international publication has been modified by common modifications, indicated by (mod), the relevant EN/HD
applies.

Publication

Year

IEC 60068-2-10

-

1)

IEC 61300-1

-

1)

IEC 61753-1

200X

1)
2)
3)

Undated reference.
Valid edition at date of issue.
To be published.

Title

3)

EN/HD

Year

Environmental testing
EN 60068-2-10
Part 2-10: Tests - Test J and guidance: Mould
growth

2005

2)

Fibre optic interconnecting devices and
passive components - Basic test and
measurement procedures
Part 1: General and guidance

EN 61300-1

2003

2)

Fibre optic interconnecting devices and
passive components
Part 1: General and guidance for
performance standards

-

-


blank

Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012


Licensed copy: Bradford University, University of Bradford, Version correct as of 21/03/2012 14:33, (c) The British Standards Institution 2012

BS EN
61300-2-16:
2006

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