Agilent infinitylab lc series vialsamplers (g7129a b c) user manual

Agilent InfinityLab LC Series

Vialsamplers

User Manual

Agilent Technologies


Notices
© Agilent Technologies, Inc. 2015-2018

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document that conflict with these
terms, the warranty terms in the separate agreement shall control.

Manual Part Number
G7129-90010 Rev. D

Edition
08/2018
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn

receive no greater than Restricted Rights as
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1987). U.S. Government users will receive
no greater than Limited Rights as defined in
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applicable in any technical data.

Safety Notices

C AU T I O N
A CAUTION notice denotes a

hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated conditions are fully understood and
met.

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The hardware and/or software described in
this document are furnished under a
license and may be used or copied only in
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WA R N I N G
A WARNING notice denotes a

hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated conditions are fully
understood and met.

Agilent InfinityLab LC Series Vialsamplers User Manual


In This Book:

In This Book:

This manual covers the following modules:
• Agilent 1260 Infinity II Vialsampler (G7129A)
• Agilent 1290 Infinity II Vialsampler (G7129B)
• Agilent 1260 Infinity II Vialsampler (G7129C)

1 Introduction
This chapter gives an introduction to the module and instrument overview.

2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.

3 Using the Module
This chapter provides information on how to use the module.

4 Installation of the Optional Sample Cooler or Thermostat
This chapter describes the installation of the optional sample cooler or
thermostat.

5 Preparing the Module
This chapter explains the operational parameters of the module.

6 Optimizing Performance
This chapter provides information on how to optimize the module.

7 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.

Agilent InfinityLab LC Series Vialsamplers User Manual

3


In This Book:

8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

9 Maintenance and Repair
This chapter describes the maintenance and the repair of the module.

10 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.

12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.

13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.

14 Appendix
This chapter provides addition information on safety, legal and web.

4

Agilent InfinityLab LC Series Vialsamplers User Manual


Contents

Contents

1 Introduction

9

Product Description (G7129A) 10
Features (G7129A) 11
Product Description (G7129B) 12
Features (G7129B) 13
Product Description (G7129C) 14
Features (G7129C) 15
Overview of the Module 16
Operating Principle 18
Leak and Waste Handling 28
2 Site Requirements and Specifications

35

Site Requirements 36
Physical Specifications 40
Performance Specifications 41
Specifications of the Sample Cooler 47
Specifications of the Sample Thermostat 49
Specifications of the Integrated Column Compartment
3 Using the Module

51

53

Magnets 54
Turn on/off 55
Status Indicators 57
Exchange Drawers 58
Install the Integrated Column Compartment 60

Install the Column Shelf 66
Install the External Tray 70
Install a Column (ICC) 72
Remove a Column (ICC) 76
Sample Trays 78
Choice of Vials and Caps 81
Transporting the Sampler with a Sample Cooler or Sample Thermostat Installed
Agilent Local Control Modules 86

Agilent InfinityLab LC Series Vialsamplers User Manual

84

5


Contents

4 Installation of the Optional Sample Cooler or Thermostat

89

Damaged Packaging 90
Install the Sample Cooler or Thermostat 91
Using the Sample Cooler or Thermostat in an Infinity II Sampler
5 Preparing the Module

105

Leak and Waste Handling 106

Preparing the Module 107
Solvent Information 108
Capillary Color Coding Guide 114
Installing Capillaries 115
Flow Connections to the Vialsampler
Setting up the Vialsampler 119
6 Optimizing Performance

97

117

131

Optimization for Lowest Carry-over 132
Fast Injection Cycle and Low Delay Volume
Precise Injection Volume 138
Choice of Rotor Seal 140
7 Troubleshooting and Diagnostics

136

141

Overview of the Module’s Indicators and Test Functions 142
Maintenance Functions 144
Step Commands 153
Tests 155
Troubleshooting 156
Troubleshooting Guide for the Sample Transport Assembly 158

Agilent Lab Advisor Software 161
8 Error Information

163

What are Error Messages 165
General Error Messages 166
Sampler Error Messages 172
Sample Cooler Error Messages 180
Integrated Column Compartment (ICC) Heater Error Messages

6

183

Agilent InfinityLab LC Series Vialsamplers User Manual


Contents

9 Maintenance and Repair

185

Introduction to Maintenance 186
Warnings and Cautions 187
Overview of Maintenance 189
Cleaning the Module 190
Remove and Install Doors 191
Exchange the Needle Assembly 192

Exchange the Needle Seat Assembly 197
Exchange the Sample Loop Assembly 202
Exchange the Rotor Seal 206
Exchange the Metering Seal and Piston 212
Exchange the Gripper Arm 216
Replace the Peristaltic Pump Cartridge 219
Exchange the Wash Port Assembly 222
Replace the Module Firmware 228
10 Parts and Materials for Maintenance

229

Main Assemblies 230
Standard Parts 232
Drawer Assembly 233
External Tray 235
Analytical Head Assembly (40 μL) 236
Analytical Head Assembly (100 µL) 237
2ps 6pt Injection Valve 1300 bar 238
2ps 6pt Injection Valve 800 bar 239
2ps 6pt Injection Valve 600 bar 240
Integrated Column Compartment 241
Cabinet Kit 242
Sample Thermostat 244
Standard Vialsampler Accessory Kit 246
Multi-Draw Kit 248
Additional Part List 249
Column Identifier Kit 2C (G4751A) 250

Agilent InfinityLab LC Series Vialsamplers User Manual

7


Contents

11 Identifying Cables

251

Cable Overview 252
Analog Cables 254
Remote Cables 256
CAN/LAN Cables 260
RS-232 Cables 261
USB 262
12 Hardware Information

263

Firmware Description 264
Electrical Connections 267
Interfaces 269
Setting the 6-bit Configuration Switch 277
Instrument Layout 281
Early Maintenance Feedback (EMF) 282
13 LAN Configuration

283

What You Have to Do First 284
TCP/IP parameter configuration 285
Configuration Switches 286
Initialization Mode Selection 287
Dynamic Host Configuration Protocol (DHCP)
Manual Configuration 292
PC and Agilent ChemStation Setup 297
14 Appendix

289

307

General Safety Information 308
Waste Electrical and Electronic Equipment (WEE) Directive
Refrigerant 315
Radio Interference 318
Sound Emission 319
Solvent Information 320
Agilent Technologies on Internet 321

8

314

Agilent InfinityLab LC Series Vialsamplers User Manual


Agilent InfinityLab LC Series Vialsamplers User Manual

1
Introduction
Product Description (G7129A)
Features (G7129A)

11

Product Description (G7129B)
Features (G7129B)

12

13

Product Description (G7129C)
Features (G7129C)

10

14

15

Overview of the Module

16

Operating Principle 18
Sampling Sequence 18
Needle Parkstation 23

Hydraulic Box 24
Transport Assembly 26
Leak and Waste Handling
Leak Sensor 32
Waste Guidance 32
Waste Concept 33

28

This chapter gives an introduction to the module and instrument overview.

Agilent Technologies

9


1

Introduction
Product Description (G7129A)

Product Description (G7129A)

The Agilent 1260 Infinity II Vialsampler is designed for the reliability, safety,
and ease-of-use needed for routine pharmaceutical tasks and quality control,
as well as for environmental and food analyses. It can house optionally the
integrated column compartment for two LC columns with temperature control
up to 80 °C as well as a sample cooler/sample thermostat for stable
temperatures down to 4 °C or from 4 °C up to 40 °C, all within one module.

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Figure 1

10

Overview of the Vialsampler

Agilent InfinityLab LC Series Vialsamplers User Manual


1

Introduction
Features (G7129A)

Features (G7129A)

• Reliable injections from 0.1 μL to 100 μL
• Capacity for up to 132 vials (2 mL)
• Easy adaption for injection volumes up to 1800 μL for applications ranging
from microbore to semipreparative chromatography
• Includes a needle flush port for rinsing of outside needle to maintain lowest

carryover during routine work
• Integrated column compartment as option or upgrade available, holding
two columns up to 30 cm length, and providing heating capacity up to 80 °C
for reproducible chromatography data at optimized resolution
• Integrated sample cooler as option or upgrade available, providing high
cooling capacity for all vials loaded down to 4 °C
• Integrated sample thermostat as option or upgrade available, providing
high cooling and heating capacity for all vials loaded from 4 °C to 40 °C
• Low internal volume for minimum contribution to a system's total internal
volume, which can be even further reduced using "bypass" mode
• Overlapped injections for increased productivity
• Injection program available for customizing advanced injections as well as
for sample preparation steps upfront injection

Agilent InfinityLab LC Series Vialsamplers User Manual

11


1

Introduction
Product Description (G7129B)

Product Description (G7129B)

The Agilent 1290 Infinity II Vialsampler is designed for UHPLC applications
up to 1300 bar. It provides the reliability, safety, and ease-of-use needed for
routine pharmaceutical tasks and quality control, as well as for environmental
and food analyses. It can house optionally the integrated column compartment

for two LC columns with temperature control up to 80 °C as well as a sample
cooler/sample thermostat for stable temperatures down to 4 °C or from 4 °C
up to 40 °C, all within one module.

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'UDZHU

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/HDNGUDLQ
3RZHUVZLWFK

Figure 2

12

Overview of the Vialsampler

Agilent InfinityLab LC Series Vialsamplers User Manual


1

Introduction
Features (G7129B)

Features (G7129B)

• Reliable injections from 0.1 μL to 20 μL

• Capacity for up to 132 vials (2 mL)
• Easy adaption for injection volumes up to 1500 μL for applications ranging
from microbore to semipreparative chromatography
• Includes a needle flush port for rinsing of outside needle to maintain lowest
carryover during routine work
• Integrated column compartment as option or upgrade available, holding
two columns up to 30 cm length, and providing heating capacity up to 80 °C
for reproducible chromatography data at optimized resolution
• Integrated sample cooler as option or upgrade available, providing high
cooling capacity for all vials loaded down to 4 °C
• Integrated sample thermostat as option or upgrade available, providing
high cooling and heating capacity for all vials loaded from 4 °C to 40 °C
• Low internal volume for the minimum contribution to a system's total
internal volume, which can be even further reduced using "bypass" mode
• Overlapped injections for increased productivity
• Injection program available for customizing advanced injections as well as
for sample preparation steps upfront injection

Agilent InfinityLab LC Series Vialsamplers User Manual

13


1

Introduction
Product Description (G7129C)

Product Description (G7129C)

The Agilent 1260 Infinity II Vialsampler is designed for the reliability, safety,
and ease-of-use needed for routine pharmaceutical tasks and quality control,
as well as for environmental and food analyses. It can house optionally the
integrated column compartment for two LC columns with temperature control
up to 80 °C as well as a sample cooler/sample thermostat for stable
temperatures down to 4 °C or from 4 °C up to 40 °C, all within one module.

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1HHGOHDVVHPEO\
'UDZHU

&ROXPQVKHOI
/HDNGUDLQ
3RZHUVZLWFK

Figure 3

14

Overview of the Vialsampler

Agilent InfinityLab LC Series Vialsamplers User Manual


1

Introduction
Features (G7129C)

Features (G7129C)

• Reliable injections from 0.1 μL to 100 μL
• Capacity for up to 132 vials (2 mL)
• Easy adaption for injection volumes up to 1800 μL for applications ranging
from microbore to semipreparative chromatography
• Includes a needle flush port for rinsing of outside needle to maintain lowest
carryover during routine work
• Integrated column compartment as option or upgrade available, holding
two columns up to 30 cm length, and providing heating capacity up to 80 °C
for reproducible chromatography data at optimized resolution
• Integrated sample cooler as option or upgrade available, providing high
cooling capacity for all vials loaded down to 4 °C
• Integrated sample thermostat as option or upgrade available, providing
high cooling and heating capacity for all vials loaded from 4 °C to 40 °C
• Low internal volume for minimum contribution to a system's total internal
volume, which can be even further reduced using "bypass" mode
• Overlapped injections for increased productivity
• Injection program available for customizing advanced injections as well as
for sample preparation steps upfront injection

Agilent InfinityLab LC Series Vialsamplers User Manual

15


1

Introduction
Overview of the Module

Overview of the Module

The Agilent InfinityLab LC Series Vialsamplers are designed for use with other
modules of the Agilent InfinityLab LC Series, 1200 Series, and 1100 Series LC,
or with other LC systems if adequate remote control inputs and outputs are
available. The vialsamplers can be controlled by Agilent Local Controllers or
by Agilent control software (OpenLAB CDS, Masshunter etc.).
Three sample-rack sizes are available for the vialsampler. The standard
half-size rack holds 2× 66 vials (2.0 mL) while the other half-size rack options
provide space for 2× 18 vials (6.0 mL) respectively. Any two half-size rack
drawers can be installed in the vialsampler simultaneously. A specially
designed two half-size sample-rack holding 2× 50 vials (2.0 mL) vials is
available for method compatibility. These special 2× 50 vials (2.0 mL) half-size
racks are not designed for combinations with the other rack portfolio.
The vialsamplers transport mechanism uses an X-Z-Theta movement to
optimize vial pick-up and return. Vials are picked up by the gripper arm, and
positioned below the needle station. The gripper transport mechanism, the
needle station, and the hydraulic unit are driven by motors. Movement is
monitored by optical sensors and optical encoders to ensure correct
operation. The metering device is always flushed after injection to ensure
minimum carry-over.
The standard analytical head of the metering device provides injection
volumes from 0.1 – 100 μL for the 1260 Infinity II Vialsampler and 0.1 – 20 μL
for the 1290 Infinity II Vialsampler version. The 1260 Infinity II Vialsampler
can be operated at up to 600 bar (G7129A) or 800 bar (G7129C) while the 1290
Infinity II Vialsampler has a pressure limit up to 1300 bar. A head with
extended volume is available for injection volumes from 0.1 – 900 μL and can
be operated up to 400 bar.
The six- port injection valve unit (only 5 ports are used) is driven by a highspeed hybrid stepper motor. During the sampling sequence, the valve unit

bypasses the vialsampler, and directly connects the flow from the pump to the
column. During injection and analysis, the valve unit directs the flow through
the vialsampler. This ensures that all the sample is completely injected into
the column. Any sample residue is removed from the metering device and from
the needle before the next sampling sequence begins.

16

Agilent InfinityLab LC Series Vialsamplers User Manual


Introduction
Overview of the Module

1

For applications that require control of the vial temperature, the module can
be combined with the Agilent 1290 Infinity Sample Cooler (G7167-60005) or
with a Sample Thermostat (G7167-60101). The combination of the Vialsampler
with the sample cooler is called a "cooled Vialsampler", the combination of the
Vialsampler with the sample thermostat is called a "thermostated
Vialsampler". For more details, refer to the 1290 Infinity Sample Cooler or
Sample Thermostat documentation.

Agilent InfinityLab LC Series Vialsamplers User Manual

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1

Introduction
Operating Principle

Operating Principle

Sampling Sequence
The Vialsampler processor continuously monitors the movements of the
vialsampler components during the sampling sequence. The processor defines
specific time windows and mechanical ranges for each movement. If a specific
step of the sampling sequence can’t be completed successfully, an error
message is generated.
During the sampling sequence, the solvent bypasses the vialsampler via the
injection valve. The gripper arm selects the sample vial, either from a static
sample rack, or from external vial positions. The gripper arm places the
sample vial below the injection needle. The required volume of sample is
drawn into the sample loop by the metering device. Sample is applied to the
column when the injection valve returns to the mainpass (main path) position
at the end of the sampling sequence.
The sampling sequence occurs in the following order:
1 The injection valve switches to the bypass position.
2 The piston of the metering device moves to the initialization position.
3 The gripper arm moves from the home position, and selects the vial. At the
same time, the needle lifts out of the seat.
4 The gripper moves into the needle station and stops in the draw position.1
5 The needle lowers into the vial.
6 The metering device draws the defined sample volume.
7 The needle lifts out of the vial.
8 The gripper arm moves out slightly and stops in the wash position1.
9 The needle moves downwards and dips into the wash well of the wash port.

Simultaneously the peristaltic pump delivers the flush solvent.1
10 The needle moves back.1

1

18

only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the
sample vial directly below the needle (Step 4) and lowers the needle into the vial.

Agilent InfinityLab LC Series Vialsamplers User Manual


Introduction
Operating Principle

1

11 The gripper arm moves out of the needle station and the wash port snaps
back in position.
12 The gripper arm replaces the vial, and returns to the home position.
Simultaneously, the needle lowers into the seat.
13 The injection valve switches to the mainpass (main path) position.

Injection Sequence
Before the start of the injection sequence, and during an analysis, the injection
valve is in the mainpass (main path) position (see Figure 4 on page 19). In this
position, the mobile phase flows through the autosamplers metering device,
sample loop, and needle. This ensures that all parts in contact with sample are
flushed during the run, thus minimizing carry-over.

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Mainpass (main path) Position - standard position during runs and when the
sampler is idle

Agilent InfinityLab LC Series Vialsamplers User Manual

19


1

Introduction
Operating Principle
When the sample sequence begins, the valve unit switches to the bypass

position (see Figure 5 on page 20). Solvent from the pump enters the valve
unit at port 1, and flows directly to the column through port 6.

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Figure 5

20

Valve in bypass - needle in vial, metering device aspirates sample volume

Agilent InfinityLab LC Series Vialsamplers User Manual


Introduction
Operating Principle

1

Then the vial is positioned below the needle. The needle moves down into the
vial, the metering unit draws the required sample volume into the loop, and
the needle is raised. In the next step, the needle is washed (see Figure 6 on
page 21).

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Figure 6

Outer face of needle getting washed in wash port

Agilent InfinityLab LC Series Vialsamplers User Manual

21

1

Introduction
Operating Principle
When the metering unit has drawn the required volume of sample into the
sample loop, the vial is replaced in the sample tray. The wash port flips into
the origin position, the needle is lowered into the needle seat, and the
injection valve switches back to mainpass (main path) position, flushing the
sample onto the column (see Figure 7 on page 22).

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Figure 7

22

Valve switches to mainpass (main path) - sample is transferred towards the
LC column

Agilent InfinityLab LC Series Vialsamplers User Manual


1

Introduction
Operating Principle

Needle Parkstation
The needle parkstation comprises two main assemblies: needle drive and wash
port.

Figure 8

Needle Station

Needle-Drive
The needle movement is driven by a stepper motor connected to the spindle
assembly by a toothed belt. The circular motion of the motor is converted to
linear motion by the drive nut on the spindle assembly. The upper and lower
needle positions are detected by reflection sensors on the needle station
board, while the needle-in-vial position is determined by counting the motor
steps from the upper needle-sensor position.

Agilent InfinityLab LC Series Vialsamplers User Manual

23

1

Introduction
Operating Principle

Hydraulic Box
The hydraulic box comprises two main assemblies: metering device, and
injection valve.

Figure 9

NOTE

Hydraulic Unit

The replacement hydraulic box excludes the injection valve and metering head assemblies.

Analytical Head
The analytical head is driven by the stepper motor that is connected to the
drive shaft by a toothed belt. The drive nut on the spindle converts the circular
movement of the spindle to linear motion. The drive nut pushes the sapphire
piston against the tension of the spring into the analytical head. The base of
the piston sits on the large bearing of the drive nut, which ensures the piston
is always centered. A ceramic ring guides the movement of the piston in the
analytical head. The home position of the piston is sensed by an optical sensor
on the hydraulic unit board while the sample volume is determined by
counting the number of steps from the home position. The backward
movement of the piston (driven by the spring) draws sample from the vial.

24

Agilent InfinityLab LC Series Vialsamplers User Manual


Introduction
Operating Principle

1

Injection Valve
The two-position 6-port injection valve is driven by a stepper motor. Only five
of the six ports are used (port 3 is not used). A lever/slider mechanism
transfers the movement of the stepper motor to the injection valve. Two
microswitches monitor switching of the valve (bypass and mainpass (main
path) end positions). No valve adjustments are required after replacing
internal components.

Agilent InfinityLab LC Series Vialsamplers User Manual

25