Collaboration for Agriculture & Rural Development:" Field evaluation and advanced vegetative mass-propagation technology for scaling up high-value plantations of Pinus caribaea and related hybrids in Vietnam - Milestone 4" pptx

Ministry of Agriculture & Rural Development
Collaboration for Agriculture & Rural Development




Project 033/05 VIE
Field evaluation and advanced vegetative mass-propagation technology for scaling
up high-value plantations of Pinus caribaea and related hybrids in Vietnam



Milestone 4:
Output 1.2: Genetic Evaluation Trials


Deliverables: Means of verification for payment
• At least 5 genetic evaluation trials sites established to compare performance of locally
available and introduced pines
• Trial protocol documented and responsibilities for long term management and research
evaluation analysis assigned


Report Title:

Commencement report for trials established in 2006 to compare
performance of local and introduced tropical pines in Vietnam

INTRODUCTION

CARD Project 033/VIE seeks to review existing experience with and performance of several
tropical conifer species in Vietnam, particularly Caribbean Pine, and to establish further trials of
improved genetic material, including hybrids.
The project will also provide capacity building for Vietnamese forest research institutions
relating to Pinus tree improvement and mass propagation systems via training in Queensland and
Vietnam, development of small-scale hedge and nursery infrastructure, and a study tour to
Australia, involving innovative Vietnamese forest managers and researchers.
Finally, the project will build awareness among large and small-scale forest growers including
ethnic minorities, through the establishment of collaborative demonstration plantings in priority
regions for Pinus plantation expansion, supported by appropriate training.
This report relates to Output 1.2 of the Project: “Establish genetic evaluation trials to compare
performance of locally available and introduced pines (especially Pinus caribaea and hybrids)
on multiple sites throughout North Central, Central Coast and Central Highlands regions of
Vietnam.

PROGRESS
Description of Sites
Table 1 describes key features of six sites where genetic evaluation trials have been established.
A seventh site near Dong Ha on the Central Coast is proposed for planting however
arrangements have not yet been finalised.
Photos for some of the sites are included in Appendix 3.
Genetic Material Treatments
Table 2 lists the range of genetic material treatments used at each site. Generally speaking,
plants for the trials were raised at the RCFTI Nursery in Cam Quy, Ba Vi for sites in northern
Vietnam and at the Cam Ly Nursery, Lam Dong Forest Research Centre for the Central
Highlands sites.
Additional local treatments were sourced from RCFTI (local Pinus caribaea var. hondurensis)
and from local nurseries (Pinus merkussii).
Experimental design and layout
Generally speaking, the trials were laid out as 6 row x 6 tree (36 tree) plots for each treatment,

arranged as a Randomised Complete Block (RCB) design with 4 replications. Appendix 1
includes a site map for each site.
Early survival, early growth and re-filling
Following early losses, some sites were re-filled with plants within the first couple of months of
planting. In addition, some sites have recently been assessed for survival and early age height
growth. Results will be reported at a later stage.
Protocols for trial management, data collection, analysis and reporting
The agency responsible for overall co-ordination of the trials is the Research Centre for Forest
Tree Improvement (RCFTI) based in Hanoi. The nominated contact person is:
Name: Mr Phi Hong Hai
Position title: Deputy-director of RCFTI
Contact Details: Phone No. 844 913309746/ 844 8389813
Email:
Responsibilities for this position include:
• Subject to availability of funding, co-ordinate trial assessments, based broadly on the
schedule listed below:
(a) Year 1: Survival, Height (cm)
(b) Year 3: Survival, Height (cm), Diameter at breast height over bark (DBHOB)
(c) Year 5: Survival, Height (dm), DBHOB (cm), Tree form & health assessment
(d) Year 10: Survival, Height (dm), DBHOB (cm), Tree form & health assessment
• Enter data into an electronic database for secure storage
• Co-ordinate data analysis and reporting using existing resources. Australian partners may be
able to assist.
• Distribute results and reports to site managers and Australian Team Leader
Appendix 2 lists the names of local site managers responsible for management of the relevant
trial site. Responsibilities include:
• Protect trials from damaging or competing agents such as over-topping weeds, animal
browsing, fire and firewood collection;
• Monitor health of trials and report any issues of interest (eg pest or insect damage, wildfire,
general decline in tree health, interference by other forest users); and

• Provide local assistance to assess trials as requested by RCFTI Co-ordinator

FUTURE DIRECTIONS
Future work required for this series of trials includes:
• Plant remaining site at Dong Ha when weather conditions are suitable
• Update this report to include Dong Ha details when confirmed
• Collate and report early age growth and survival data for the various trials
• Continue to maintain trial sites free from competing weeds and other interference
• Periodic assessment and reporting as per responsibilities outlined in this document

Table 1: Description of Pinus genetic evaluation sites planted in 2006
Site 1 Site 2 Site 3 Site 4 Site 5 Site 6
Province
Ha Tay Vinh Phuc Vinh Phuc Lam Dong Gia Lai Quang Ninh
Region
North North North Central Highlands Central Highlands North
Locality
Cam Quy, Ba Vi Lap Thach Dai Lai Lang Hanh Pleiku Hoanh Bo
Agency
RCFTI Lap Thach District
Forest Enterprise,
part of Vinapaco
Phu Ninh FRC Lam Dong FRC Gia Lai Tropical
Forestry Centre
Non-timber Forest
Research Centre
Latitude
21° 07’N 21° 29’ 46’’N 21° 20’ 36’’N 11° 38’ 15’’N 13° 59’N 20
o
54’

Longitude
105° 26’E 105° 27’ 5’’E 105° 43’50’’E 108° 15’53’’E 108° 00’E 106
o
50’
Elevation
(m)
50 75 37-95 933 800 200
Annual
Rainfall
1,680 1,387 1,500 1,730 2,270 2,061
Soil
Poor soil, heavy clay
at 50cm
Reasonable, yellow
medium clay
Yellow medium
clay + quartz
outcrops
Deep, red, well-
drained
Deep, red, well-
drained
Ferralit, medium to
deep soil layer, red
– yellow
Date planted
August 2006 29 July 2006 31 August 2006 7 July 2006 July 2006 31 August 2006
Cultivation
Ripping to 70cm +
40cmx40cmx40cm

manual pit
cultivation
40cmx40cmx40cm
manual pit
cultivation
Fertiliser mixed in
40cmx40cmx40cm
manual pit
cultivation
30cmx30cmx30cm
manual pit
cultivation
Fertiliser mixed in
30cmx30cmx30cm
manual pit
cultivation Fertiliser
mixed in
40cmx40cmx40cm
manual pit
cultivation
Fertiliser mixed in
Fertiliser
200gm
superphosphate/tree
200gm
superphosphate/tree
200gm
superphosphate/tree
200gm
superphosphate/tree

200gm
superphosphate/tree
200gm
superphosphate/tree
Tree spacing
3mx3m (1,111/ha) 3mx3m (1,111/ha)

3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha)

Table 2: Genetic treatments included in 2006 plant Pinus genetic evaluation trials: Number = treatment number for particular trial

Site 1 Site 2 Site 3 Site 4 Site 5 Site 6
Province Ha Tay Vinh Phuc Vinh Phuc Lam Dong Gia Lai Quang Ninh
Locality Ba Vi Lap Thach Dai Lai Lang Hanh Pleiku Hoanh Bo
Pinus massoniana (PMASS) – Tam Dao 1 1 1 N/A N/A 1
P. caribaea var. caribaea (PCC) - Thua Thien Hue 2 2 2 2 2 12
P. caribaea var. hondurensis (PCH) – non select – Dai Lai 3 3 3 8 8 9
P. caribaea var. hondurensis (PCH) –select – elite seed
from 11 plus trees, Dai Lai SPA
444338
P. caribaea var. hondurensis (PCH) – non select –
Queensland
555446
P. caribaea var. hondurensis (PCH) –select – Queensland 6 6 N/A 5 5 7
P. caribaea var. hondurensis (PCH) –Cam Quy – non-
select
7
P. caribaea var. hondurensis (PCH) – FRC source N/A 7 N/A N/A N/A N/A
P. elliottii var. elliottii x PCH = PEE x PCH F2 hybrid -
Queensland

887775
PCH x P. caribaea var. bahamensis (PCH x PCB) -
Queensland
9 9 N/A N/A N/A 3
PCH x P. caribaea var. caribaea (PCH x PCC) –
Queensland
10 10 8 6 6 2
PEE x PCH Series II clones
(a) clone c3640, bare root cuttings
(b) clone c 3136, bare root cuttings
(c) clone c 4124, bare root cuttings
11
11a
11c
11b
11
11a
11b
11c
N/A N/A N/A 10
10a

10b (11)
Pinus merkussii (PMERK) N/A N/A N/A N/A N/A N/A
P. caribaea var. bahamensis – Cam Quy 1 N/A 6 N/A N/A N/A
P. caribaea var. bahamensis – Qld 4
P. kesiya (PKES) – non select - Dalat N/A N/A N/A 1 1 N/A
P. kesiya (PKES) – select - Dalat N/A N/A N/A 9 9 N/A

N/A = Not Applicable to this site

Appendix 1: Site Design maps for CARD Pinus genetic evaluation trials established in 2006

Site Number Location Province Design attached
1 Cam Quy, Ba Vi Ha Tay Yes
2 Lap Thach Vinh Phuc See Excel file
3 Dai Lai Vinh Phuc Yes
4 Lang Hanh Lam Dong Yes
5 Pleiku Gia Lai Yes
6 Hoanh Bo Quang Ninh Yes
7 Dong Ha Quang Tri Not yet

SITE 1: Experimental design for Pinus caribaea & hybrid pine
at Cam Quy, Ba Vi – Ha Tay (Vietnam)
(Part of CARD 033/05 VIE Project)

+ Date of planting: August 2006
+ Trial design: RCB with 6 rows and 6 columns
+ Treatments: 11
+ Replications: 4
+ Trees/ plot: 36
+ Planted spacing: 3 x 3 m
+ Hole: 40 x 40 x 40 cm
+ Fertilizer: 200 gram Super Phosphate/ plot
+ Total area: 2 ha(experimental area - 1.5 ha)



DT1
Dai Lai DT2

(no select) QLQ
(non select) 4:mass
10.c 15.c 34.c
11 6 5 1 2 9
cab
10 8 3 7 4
11 8 7 3 5 4 1 9 2 6 10
b c a
11 3 2 4 10 8 6 7
bac
9 5 1
11 1 5 9
a b c
7 2 6 10 4 8 3
Outside border, 1 row, 4 P.massoniana

Acacia hybrid

Note:
Code Species Provernance
1
P.massoniana
PMASS TamDao
2 P.caribaea var caribaea PCC ThuaThienHue
3 P.caribaea var hondurensis PCH Dai Lai (Not selected)
4 P.caribaea var hondurensis PCH Dai Lai (Selected)
5 P.caribaea var hondurensis PCH Queensland (Not selected)
6 P.caribaea var hondurensis PCH Queensland (Selected)
7 P.caribaea var hondurensis PCH CamQuy (Not selected)
8 P.elliottii x PCH F2 PEE x PCH Queensland

9 P.caribaea var hondurensis x
P.caribaea var bahamensis
PCH x PCB Queensland
10 P.caribaea var hondurensis x
P.caribaea var caribaea
PCH x PCC Queensland
11 P.elliottii xPCH F1 clone cutting PEE x PCH cutting Queensland
11a= C4124, 11b= C3640, 11c= C3136

SITE 2: Experimental design for Pinus caribaea and hybrid pine
at Lap Thach – Vinh Phuc (Vietnam)

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3

11111111111111111111111111 1 111111112222222222222222222222222222222222223
11111111111111111 222222222222222222222223
11111111111111111 222222222222222223
111111111111111 222222222
111111111
1111
1 2 3 4 5 6 7 8 9 101112131415161718192021 22 23242526272829303132333435363738394041424344454647484950515253545556575859606162636465666768
1 Pinus massoniana - PMASS
X3mXXXXX
2 Pinus caribaea var caribaea - PCC ( Thõa Thiªn Hu
Õ
)
3m
3 PCH (no select) Dai Lai
XXXXXX
4 PCH (select) Dai Lai
5 PCH (no select) Queensland
XXXXXX
6 PCH (select) Queensland
15m
7 PCH - FRC source
XXXXXX
8PEE x PCH F2 (Queensland)
9 PCH x PCB (Queensland) C3640
XXXXXX
10 PCH x PCC (Queensland)
11 PEE x PCH cutting (Queensland) C3136
XXXXXX
C4124
Experimental design for

P
inus caribaea
and hybrid pine
a t Ng o c My - La p Tha c h Fo re s try En te rpris e - P hu Th o
Date planted: 29 July 2006
(As a part of CARD 033/05 VIE Project)
North dire ction
(b)
(
c
)
Xuan Hoa vil. 12 km
Highway 2C 4 km
15m
Road
(a)
Rep.
I
Rep.
II
Rep.
III
Rep.
IV
6
11 8
7
51 1024
3
9

111
79
63105
2
8
4
95
11 8 2
1
6310
725869310
1
7
4
411
Terrace Field of Cassava

SITE 3: Experimental design for Pinus caribaea and hybrid pine
at Dai Lai – Vinh Phuc (Vietnam)
(Part of CARD 033/05 VIE Project)

+ Date of planted: 31 August 2006
+ Design as randomized complete block with 2 rows and 4 columns
+ Treatments: 8
+ Replications: 4
+ Trees/ plot: 36
+ Total area: 1.02 ha (with experimental area: 0.54 ha)


7

3
1 8
6 2
5 4
7 6
5 8
1
3
4 2
7 4
3 5
2
8
6
7 1
4
6
2
5
3
8
1
Rep. 4
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
Rep. 2

Rep. 1
1 edge row
P
. mass TDao
5 rows (4)
P
C
C
-Hue
4 rows (3)
P
. mass- TDao
1 edge row
P
. mass TDao
1 edge row
P
. mass TDao
1 edge row
P
. mass TDao
1 edge row
P
. mass TDao
1 edge row
P
. mass TDao
PCC-Hue
(
4

)
PCH (No select)
Dai Lai
(
1
)
P. mass
T
D
ao
(
3
)

PCH (no
select
)

Treatments:
In genetic trial
1. P. massoniana Tam Dao
2. PCC – Hue
3. PCH (no select) Dai Lai
4. PCH (select) Dai Lai
5. PCH (no select) QLD
6. PCB Cam Quy (Ha Tay)
7. PEE x PCH (F2) QLD
8. PCH x PCC (F1) QLD
(1 8 is tree code in layout)
On buffer zone

1. PCH (no select) Dai Lai
2. PCH (no select) QLD
3. P.Mass Tam Dao
4. PCC Hue
(Note: plot 4 & 5 of Rep.4
destroyed by illegal harvest by
local people and re-planted
with A. mangium)
Rep. 3

SITE 4: EXPERIMENTAL DESIGN FOR PINUS CARIBAEA AND HYBRID PINE AT
LANG HANH – LAM DONG
(Part of CARD 033/05 VIE Project)
(Date planted: July 2006)

1 2 3 4 5 6
7 8 9 10 11 12
13 14 15 16 17 18
19 20 21 22 23 24
25 26 27 28 29 30
31 32 33 34 35 36
36 trees/plot

1

2 3

5m
8 5 6 2 4 1

4

5 6 7 4 9 3 7 5

7

8 9 3 2 1 8 6 9
Rep. 1 Rep. 2 Rep. 3
Road among plots 5m


3

6 1 7 8 9 5 4 2

Rep. 4

Road 5m


Treatments:
1. P.kesiya (no select) Da Lat (PKES)
2. P.caribaea var caribaea – Hue (PCC)
3. P.caribaea var hondurensis (select) Dai Lai
4. P.caribaea var hondurensis (no select) Queensland
5. P.caribaea var hondurensis (select) Queensland
6. P.caribaea var hondurensis x P.caribaea var caribaea – Queensland (PCH x PCC)
7. P.elliottii var. elliottii x PCH F
2
Queensland (PEE x PCH)
8. P.caribaea var hondurensis (no select) Dai Lai (PCH)
9. P.kesiya (select) Da Lat
SITE 5: EXPERIMENTAL DESIGN FOR PINUS CARIBAEA AND HYBRID PINE

AT GIA LAI - PLEIKU
(Part of CARD 033/05 VIE Project)
(Date of planted: July 2006)

P.kesiya trial planted 1986


1 2 3 4 5 6
7 8 9 10 11 12
13 14 15 16 17 18
19 20 21 22 23 24
25 26 27 28 29 30
31 32 33 34 35 36

36 trees/plot

1

2 3

8 5 6

2 4 1 9 4 7

4

5 6 7 4 9 3 7 5 6 1 5

7

8 9 3 2 1 8 6 9 3 8 2
Rep. 1 Rep. 2 Rep. 3 Rep.4





Fence


Treatments:
1. P.kesiya (no select) Da Lat (PKES)
2. P.caribaea var caribaea – Hue (PCC)
3. P.caribaea var hondurensis (select) Dai Lai
4. P.caribaea var hondurensis (no select) Queensland
5. P.caribaea var hondurensis (select) Queensland
6. P.caribaea var hondurensis x P.caribaea var caribaea – Queensland (PCH x PCC)
7. P.elliottii var. elliottii x PCH F
2
Queensland (PEE x PCH)
8. P.caribaea var hondurensis (no select) Dai Lai (PCH)
9. P.kesiya (select) Da Lat
SITE 6: Experimental design for Pinus caribaea and hybrid pine
at Hoanh Bo – Quang Ninh (Vietnam)
(Part of CARD 033/05 VIE Project)

+ Date planted: 24 July 2006
+ Design as randomized complete block with 2 rows and 5 columns
+ Treatments: 10
+ Replications: 4

+ Trees/ plot: 36
+ Total area: 2 ha (with experimental area: 1.35 ha)
+ Planted spacing: 3 x 3 m
+ Hole: 40 x 40 x 40 cm
+ Fertilizer: 200 gram Super Phosphate/ plot

















P. mas TD
1 8 2 5 7
6 9 10 3 4
10
8 1 3 7
5 6 4 2 9
4 3 8 6 7
2 1 10 9 5

4 2 3 8 10
5 6
1
9 7
PCH DL
No -select
PCH Qld
No-select
PCC Hue

Rep. 4
Rep. 3
Rep. 2
Rep. 1
Top of
hill
Road
Macadamia
Botanic Garden
Treatments:
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
♣ ♣ ♣ ♣ ♣ ♣
P. massoniana
Tam Dao
Macadamia
1. P. massoniana Tam Dao

2. PCH x PCC Queensland
3. PCH x PCB Queensland
4. PCB Queensland vµ PCC Hue
5. PEE x PCH (F2)
6. PCH Qld (No-select)
7. PCH Qld (Select)
8. PCH Dai Lai (Select)
9. PCH Dai Lai (No-select)
10. PEE x PCH (F1)-clonal cuttings includes C3640: 60 cuttings; C4124: 54 cuttings

(1 10 is code that shown in above experimental trial)

Appendix 2: Details of local Site Managers responsible for CARD Pinus genetic evaluation
trials established in 2006

Site
Number
Location Province Local Site Manager
1 Cam Quy, Ba Vi Ha Tay Mr Nguyen Dinh Hai

2 Lap Thach Vinh Phuc Mr Luc Nhu Trung

3 Dai Lai Vinh Phuc Mr Dinh Van Quang
4 Lang Hanh Lam Dong Mr Hua Vinh Tung
5 Pleiku Gia Lai Mr Nguyen Van Cuong
6 Hoanh Bo Quang Ninh Mr. Nguyen Huy Son
Appendix 3: Photos of CARD Pinus genetic evaluation trials established in 2006
(Top Left: Site 1, Cam Quy, Ba Vi; Top Right: Site 2, Lap Thach; Centre: Site 3, Dai Lai;
Bottom: Site 4, Lang Hanh)