Handbook of Microbiological Media, Fourth Edition part 101 docx

Magnetospirillum Medium 995
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Yersinia enterocolitica from foods.
Magnetic Spirillum Growth Medium, Revised
(MSGM, Revised)
Composition per liter:
Agar 1.3g
KH
2
PO
4
0.68g
Tartaric acid 0.37g
Succinic acid 0.37g
NaNO
3
0.12g
Sodium acetate 0.05g
Ascorbic acid 0.035g
Wolfe’s vitamin solution 10.0mL
Wolfe’s mineral solution 5.0mL
Ferric quinate solution 2.0mL
Resazurin (0.1% solution) 0.45mL
pH 6.75 ± 0.2 at 25°C
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl 10.0mg
Thiamine·HCl 5.0mg

Riboflavin 5.0mg
Nicotinic acid 5.0mg
Calcium pantothenate 5.0mg
p-Aminobenzoic acid 5.0mg
Thioctic acid 5.0mg
Biotin 2.0mg
Folic acid 2.0mg
Cyanocobalamin 100.0μg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Wolfe’s Mineral Solution:
Composition
per liter:
MgSO
4
·7H
2
O 3.0g
Nitriloacetic acid 1.5g
NaCl 1.0g
MnSO
4
·H
2
O 0.5g
FeSO
4
·7H
2
O 0.1g

CoCl
2
·6H
2
O 0.1g
CaCl
2
0.1g
ZnSO
4
·7H
2
O 0.1g
CuSO
4
·5H
2
O 0.01g
AlK(SO
4
)
2
·12H
2
O 0.01g
H
3
BO
3
0.01g

Na
2
MoO
4
·2H
2
O 0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L.
Ferric Quinate Solution:
Composition
per 100.0mL:
FeCl
3
0.27g
Quinic acid 0.19g
Preparation of Ferric Quinate Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: To 1.0L of distilled/deionized water add
components in the following order: Wolfe’s vitamin solution, Wolfe’s min-
eral solution, ferric quinate solution, resazurin, KH
2
PO
4
, NaNO
3
, ascorbic

acid, tartaric acid, succinic acid, sodium acetate, and agar. Mix thoroughly
after each addition. Adjust pH to 6.75 with NaOH. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically distribute into sterile screw-capped
tubes. Fill tubes to capacity with medium. Use a heavy inoculum in each
tube and do not introduce a headspace of air. Screw down caps tightly.
Use: For the cultivation and maintenance of Aquaspirillum magnetot-
acticum.
Magnetospirillum Medium
(DSMZ Medium 380)
Composition per liter:
KH
2
PO
4
0.68g
L(+)-Tartaric acid 0.37g
Succinic acid 0.37g
NaNO
3
0.12g
Na-thioglycolate 0.05g
Na-acetate 0.05g
Resazurin 0.5mg
Vitamin solution 10.0mL
Trace elements solution 5.0mL
Ferric quinate solution 2.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition
per liter:

MgSO
4
·7H
2
O 3.0g
Nitrilotriacetic acid 1.5g
NaCl 1.0g
MnSO
4
·2H
2
O 0.5g
CoSO
4
·7H
2
O 0.18g
ZnSO
4
·7H
2
O 0.18g
CaCl
2
·2H
2
O 0.1g
FeSO
4
·7H

2
O 0.1g
NiCl
2
·6H
2
O 0.025g
KAl(SO
4
)
2
·12H
2
O 0.02g
H
3
BO
3
0.01g
Na
2
MoO
4
·4H
2
O 0.01g
CuSO
4
·5H
2

O 0.01g
Na
2
SeO
3
·5H
2
O 0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition
per liter:
Pyridoxine-HCl 10.0mg
Thiamine-HCl·2H
2
O 5.0mg
Riboflavin 5.0mg
Nicotinic acid 5.0mg
D-Ca-pantothenate 5.0mg
p-Aminobenzoic acid 5.0mg
Lipoic acid 5.0mg
Biotin 2.0mg
Folic acid 2.0mg
Vitamin B
12
0.1mg
© 2010 by Taylor and Francis Group, LLC

996 Magnetospirillum 2 Medium
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H
2
+ 20% CO
2
. Filter sterilize.
Ferric Quinate Solution:
Composition per 100.0mL:
FeCl
3
·6H
2
O 0.45g
Quinic acid 0.19g
Preparation of Ferric Quinate Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Sparge with
N
2
. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except vitamin solu-
tion and ferric quinate solution, to distilled/deionized water and bring
volume to 988.0mL. Purge medium with N
2
gas for 10 min. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically and anerobically add 10.0mL vitamin solution and 2.0mL
ferric quinate solution. Mix thoroughly. Purge medium with N
2

gas for
10 min. Under the same atmosphere, anaerobically fill tubes to 1/3 of
their volume and seal. Autoclave at 121°C for 15 min. Before inocula-
tion, add sterile air (with hypodermic syringe through the rubber clo-
sure) to 1% O
2
concentration in the gas phase.
Use: For the cultivation of Magnetospirillum magnetotacti-
cum=Aquaspirillum magnetotacticum, and Magnetospirillum gryph-
iswaldense.
Magnetospirillum 2 Medium
Composition per liter:
Sodium acetate 1.0g
K
2
HPO
4
0.5g
Sodium thioglycolate 0.5g
NH
4
Cl 0.1g
Yeast extract 0.1g
Ferric citrate 20.0μg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into screw-
capped tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C. Allow medium to stand upright at room temperature for
2 to 3 days before inoculation. Do not shake.

Use: For the cultivation and maintenance of Magnetospirillum gryph-
iswaldense.
Magnetospirillum Semi-solid Medium
(DSMZ Medium 380)
Composition per liter:
Agar 1.3g
KH
2
PO
4
0.68g
L(+)-Tartaric acid 0.37g
Succinic acid 0.37g
NaNO
3
0.12g
Na-thioglycolate 0.05g
Na-acetate 0.05g
Resazurin 0.5mg
Vitamin solution 10.0mL
Trace elements solution 5.0mL
Ferric quinate solution 2.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition
per liter:
MgSO
4
·7H
2

O 3.0g
Nitrilotriacetic acid 1.5g
NaCl 1.0g
MnSO
4
·2H
2
O 0.5g
CoSO
4
·7H
2
O 0.18g
ZnSO
4
·7H
2
O 0.18g
CaCl
2
·2H
2
O 0.1g
FeSO
4
·7H
2
O 0.1g
NiCl
2

·6H
2
O 0.025g
KAl(SO
4
)
2
·12H
2
O 0.02g
H
3
BO
3
0.01g
Na
2
MoO
4
·4H
2
O 0.01g
CuSO
4
·5H
2
O 0.01g
Na
2
SeO

3
·5H
2
O 0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition
per liter:
Pyridoxine-HCl 10.0mg
Thiamine-HCl·2H
2
O 5.0mg
Riboflavin 5.0mg
Nicotinic acid 5.0mg
D-Ca-pantothenate 5.0mg
p-Aminobenzoic acid 5.0mg
Lipoic acid 5.0mg
Biotin 2.0mg
Folic acid 2.0mg
Vitamin B
12
0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H
2
+ 20% CO

2
. Filter sterilize.
Ferric Quinate Solution:
Composition per 100.0mL:
FeCl
3
·6H
2
O 0.45g
Quinic acid 0.19g
Preparation of Ferric Quinate Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Sparge with
N
2
. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°C.
Preparation of Medium: Add components, except vitamin solu-
tion and ferric quinate solution, to distilled/deionized water and bring
volume to 988.0mL. Purge medium with N
2
gas for 10 min. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°C.
Aseptically and anerobically add 10.0mL vitamin solution and 2.0mL
ferric quinate solution. Mix thoroughly. Purge medium with N
2
gas for
10 min. Dispense 12mL of medium per 16 x 150mm anaerobe screw-
cap tube under N
2
gas. Prior to inoculation, remove caps briefly under
air, tighten the caps again, and wait several hours to establish oxygen

gradients. The medium should be slightly pink in color. Strongly re-
duced conditions will not support growth of the organism. During
growth O
2
will be consumed, resazurin decolorized, and the pH in-
creased. Feed oxygen (by adding air) and succinic acid from sterile
0.05M solution (to maintain pH below 7.0). If higher densities of mag-
netic cell are wanted, ferric quinate also can be fed. For transfer use cell
material which has been concentrated at the glass wall of the culture
vessel by means of a magnetic rod attached outside.
© 2010 by Taylor and Francis Group, LLC
Maleate Medium for Pseudomonas fluorescens with Glucose and Phenol Red 997
Use: For the cultivation of Magnetospirillum magnetotacticum
(Aquaspirillum magnetotacticum) and Magnetospirillum gryphiswald-
ense.
Maintenance HiVeg Medium for B. subtilis ATCC
6633
Composition per liter:
Agar 15.0g
Plant peptone 6.0g
Plant hydrolysate 4.0g
Yeast extract 3.0g
Plant extract 1.5g
Glucose 1.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Bacillus subtilis.
Maintenance of L Antigen in Neisseria
Composition per liter:
Proteose peptone No. 3 20.0g
Agar 15.0g
Na
2
HPO
4
5.0g
NaCl 5.0g
Glucose 0.5g
Rabbit blood, defibrinated 100.0mL
pH 7.4–7.6 at 25°C
Preparation of Medium: Add components, except rabbit blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat while stirring until boiling. Autoclave for 20 min at 15
psi pressure–121°C. Cool to 60°C. Aseptically add 100.0mL of sterile,
defibrinated rabbit blood. Maintain at 75°C while shaking for 30 min.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Neisseria gonorrhoeae.
Maintenance SCY Medium
See: SCY Medium
Maintenance (SCY) HiVeg Medium
Composition per liter:
Agar 10.0g
Sucrose 1.0g
Plant hydrolysate 0.91g
Yeast extract 0.25g

NaCl 0.05g
Papaic digest of soybean meal 0.03g
K
2
HPO
4
0.02g
Thiamine 0.4mg
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Use: For the cultivation and maintenance of iron and sulfur bacteria.
Malachite Green Broth
Composition per liter:
Peptone 15.0g
Beef extract 9.0g
Malachite Green 0.01mg
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Pseudomonas aeruginosa.
Maleate Medium for Pseudomonas fluorescens
Composition per liter:
Agar 15.0g
K
2
HPO

4
1.13g
NH
4
NO
3
1.0g
KH
2
PO
4
0.48g
MgSO
4
·7H
2
O 0.2g
Potassium maleate solution 8.61mL
pH 7.0 ± 0.2 at 25°C
Potassium Maleate Solution:
Composition
per liter:
Maleic acid 116.07g
KOH (10N solution) 200.0mL
Preparation of Potassium Maleate Solution: Add maleic acid
to distilled/deionized water and bring volume to 600.0mL. Slowly add
KOH solution (generates heat). Bring volume to 1.0L with distilled/de-
ionized water. Adjust pH to 7.0. Filter sterilize.
Preparation of Medium: Add components, except potassium
maleate solution, to distilled/deionized water and bring volume to

991.4mL. Mix thoroughly. Gently heat while stirring until boiling. Au-
toclave for 20 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 8.61mL of the potassium maleate solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Pseudomonas fluorescens
and Mycoplasma pneumoniae.
Maleate Medium for Pseudomonas fluorescens
with Glucose and Phenol Red
Composition per liter:
Agar 15.0g
Glucose 10.0g
K
2
HPO
4
1.13g
NH
4
NO
3
1.0g
KH
2
PO
4
0.48g
MgSO
4
·7H
2

O 0.2g
Phenol Red 0.04g
Potassium maleate solution 8.61mL
pH 7.0 ± 0.2 at 25°C
Potassium Maleate Solution:
Composition
per liter:
Maleic acid 116.07g
KOH (10N solution) 200.0mL
Preparation of Potassium Maleate Solution: Add maleic acid
to distilled/deionized water and bring volume to 600.0mL. Slowly add
KOH solution (generates heat). Bring volume to 1.0L with distilled/de-
ionized water. Adjust pH to 7.0. Filter sterilize.
© 2010 by Taylor and Francis Group, LLC
998 Malonate Broth
Preparation of Medium: Add components, except potassium
maleate solution, to distilled/deionized water and bring volume to
991.4mL. Mix thoroughly. Gently heat while stirring until boiling. Au-
toclave for 20 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 8.61mL of the potassium maleate solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Pseudomonas fluorescens
and Mycoplasma pneumoniae.
Malonate Broth
Composition per liter:
Sodium malonate 3.0g
NaCl 2.0g
(NH
4
)

2
SO
4
2.0g
K
2
HPO
4
0.6g
KH
2
PO
4
0.4g
Bromthymol Blue 0.025g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid intro-
duction of carbon and nitrogen from other sources.
Use: For the cultivation and differentiation of coliforms and other
enteric organisms, particularly Enterobacter and Escherichia, based on
their ability to utilize malonate as the sole carbon source and ammo-
nium sulfate as the sole nitrogen source. Malonate-utilizing organisms
turn the medium blue.
Malonate Broth, Ewing Modified
Composition per liter:
Sodium malonate 3.0g

NaCl 2.0g
(NH
4
)
2
SO
4
2.0g
Yeast extract 1.0g
Glucose 0.25g
K
2
HPO
4
0.6g
KH
2
PO
4
0.4g
Bromthymol Blue 0.025g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of coliforms and other
enteric organisms, particularly Enterobacter and Escherichia, based on
their ability to utilize malonate as a carbon source and ammonium sul-

fate as a nitrogen source. The small amount of yeast extract and glu-
cose encourages the growth of some organisms that may be distressed
or fail to respond. Malonate-utilizing organisms turn the medium blue.
Malt Agar
Composition per liter:
Malt extract 30.0g
Agar 15.0g
pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–118°C. Do not overheat or agar will not harden.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of yeasts and molds.
Malt Agar
Composition per liter:
Agar 20.0g
Malt extract 12.5g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of fungi.
Malt Agar, 1/3 Strength
(ATCC Medium 2365)
Composition per liter:
Agar 15.0g
Malt extract 10.0g

pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–118°C. Do not overheat or agar will not harden.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of yeasts and molds.
Malt Agar, Blakeslee
Composition per liter:
Glucose 20.0g
Malt extract 20.0g
Agar 16.0g
Mycological peptone 1.0g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Acremonium chrysogenum,
Agaricus bisporus, Agrocybe aegerita, Armillaria mellea, Aspergillus spe-
cies, Aureobasidium pullulans, Basidiomycetes species, Candida species,
Ceratocystis adiposa, Citeromyces matritensis, Cladosporium cucumeri-
num, Cochliobolus miyaheanus, Colletotrichum destructivum, Colletotri-
chum lindemuthianum, Cryptococcus albidus, numerous other Cryptococ-
cus species, Debaryomyces polymorphus, Diaporthe magnusii, Diaporth-
ephaseolorum, Drechslera spicifera, Fusarium graminearum, Geotri-
chum lactis, other Geotrichum species, Hanseniaspora uvarum, Hanse-
niaspora valbyensis, Hansenula subpelliculosa, Humicola species,

Kloeckera apiculata, Kloeckera corticis, Kluyveromyces lodderi, Kluyver-
omyces marxianus, Metschnikowia pulcherrima, Monilinia fructigena,
© 2010 by Taylor and Francis Group, LLC
Malt Agar 4% with Lupine Stems 999
Myrothecium verrucaria, Myxozyma melibiosi, Nadsonia fulvescens, Neu-
rospora crassa, Octosporomyces octosporus, Pachysolen tannophilus,
Paecilomyces variotii, Penicillium aurantiogriseum, many other Penicil-
lium species, Pithoascus schumacheri, Rhizoctonia crocorum, Rhizomu-
cor miehei, Rhodosporidium toruloides, Rhodotorula glutinis, Rhodoto-
rula minuta, Rhodotorula rubra, Saccharomyces cerevisiae, Saccharomy-
ces exiguus, Saccharomyces ludwigii, Saccharomyces capsularis, Saccha-
romyces fibuligera, Schizosaccharomyces pombe, Sporobolomyces para-
roseus, Sporobolomyces salmonicolor, Sporopachydermia lactativora,
Stachybotrys species, Talaromyces emersonii, Taphrina deformans, Toru-
laspora delbrueckii, Trichoderma harzianum, Trichoderma koningii,
Trichoderma longibrachiatum, Trichoderma reesei, Trichoderma viride,
Trichosporon beigelii, Wickerhamia fluorescens, Yarrowia lipolytica, and
Zygosaccharomyces veronae.
Malt Agar 1%
(MA1)
Composition per liter:
Agar 15.0g
Malt extract 10.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Acrodontium griseum, Acrodontium simplex,
Acrophialophora fusispora, Acrothecium tenebrosum, Acrothecium
capsic, Agaricus bisporus, Chaetocladium jonesii, Chaetomium globo-

sum, Chaetomium cupreum, Chaetomium irregulare, Farlowiella
carmichaeliana, and many other filamentous fungi.
Malt Agar 2%
(MA2)
Composition per liter:
Malt extract 20.0g
Agar 15.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Acrophialophora fusispora, Agaricus
augustus, Ceratocystis penicillata, Chaetocladium brefeldii, and many
other fungi.
Malt Agar 4%
(MA4)
Composition per liter:
Malt extract 40.0g
Agar 15.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Chaetomium globosum, Chaetomium indi-
cum, Chaetomium funicola, Chaetomium megalocarpum, Chaetomium
murorum, Chaetomium seminudum, Chaetomium pachypodioides,
Chaetomium perlucidum, Chaetomium quadrangulatum, Chaetomium
reflexum, Chaetomium subaffine, Chaetomium subspirilliferum, Chaeto-
mium succineum, Chaetomium luknowense, Daedalea quercina,
Mycosphaerella ligulicola, Polypaecilum pisce, and many other fungi.

Malt Agar 8%
(MA8)
Composition per liter:
Malt extract 80.0g
Agar 15.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Moniliella pollinis.
Malt Agar with 0.5% CaCO
3
Composition per liter:
Malt extract 20.0g
Glucose 20.0g
Agar 15.0g
CaCO
3
5.0g
Mycological peptone 1.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Brettanomyces anomalus,
Brettanomyces bruxellensis, Brettanomyces claussenii, Brettanomyces
lambicus, Dekkera bruxellensis, and Dekkera intermedia.
Malt Agar with 2% Malt
Composition per liter:

Agar 20.0g
Malt extract 20.0g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation of a variety of yeasts and other fungi.
Malt Agar 2% with Lupine Stems
(MA2 with Lupine Stems)
Composition per liter:
Malt extract 20.0g
Agar 15.0g
Lupine stems variable
Preparation of Medium: Add components, except lupine stems, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes.
Cut lupine stems into 8.0cm-long pieces. Add 2–3 lupine stems per
tube. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For the cultivation of Chaetomium bostrychodes.
Malt Agar 4% with Lupine Stems
(MA4 with Lupine Stems)
Composition per liter:
Malt extract 40.0g
Agar 15.0g
Lupine stems variable
© 2010 by Taylor and Francis Group, LLC
1000 Malt Dextrose Peptone Agar

Preparation of Medium: Add components, except lupine stems, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes.
Cut lupine stems into 8.0cm-long pieces. Add 2–3 lupine stems per
tube. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For the cultivation of Chaetomium indicum and Chaetospermum
chaetosporum.
Malt Dextrose Peptone Agar
(MDPA)
Composition per liter:
Agar 25.0g
Malt extract 20.0g
Glucose 20.0g
Peptone 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Aspergillus aeneus, Aspergillus candidus,
Odontia uda, Oidiodendron chlamydosporicum, Oidiodendron cerealis,
Oidiodendron flavum, Oidiodendron griseum, Oidiodendron perico-
nioides, Oidiodendron tenuissimum, Penicillium spinulosum, and other
fungi.
Malt Dextrose 40% Peptone Agar
(MDPA 40)
Composition per liter:
Glucose 400.0g
Agar 25.0g
Malt extract 20.0g

Peptone 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Aspergillus penicilloides.
Malt 4% Dextrose Peptone Yeast Agar
(MDPYA4)
Composition per liter:
Malt extract 40.0g
Agar 20.0g
Glucose 20.0g
Peptone 1.0g
Yeast extract 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Candida glabrata, Candida haemulonii, Can-
dida lactis-condensi, Candida magnoliae, Candida nemodendra, Met-
schnikowia pulcherrima, Metschnikowia reukaufii, Phoma glomerata,
Saccharomyces exiguus, and Trigonopsis variabilis.
Malt 4% Dextrose Yeast Agar
(MDYA4)
Composition per liter:
Malt extract 40.0g
Agar 20.0g
Glucose 20.0g
Yeast extract 1.0g
Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Torulaspora delbrueckii.
Malt Extract Agar
Composition per liter:
Malt extract 30.0g
Agar 15.0g
Peptone 5.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 10
psi pressure–115°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Xanthomonas species.
Malt Extract Agar
(MEA)
Composition per liter:
Agar 20.0g
Glucose 20.0g
Malt extract 20.0g
Peptone 1.0g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and identification of heat-resistant
filamentous fungi (molds) from foods.
Malt Extract Agar

Composition per liter:
Malt extract 30.0g
Agar 15.0g
Mycological peptone 5.0g
pH 5.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 10
min at 15 psi pressure–115°C. Do not overheat or agar will not harden.
If a lower pH (3.5) is desired, cool medium to 55°C and aseptically add
100.0mL of sterile lactic acid. Pour into sterile Petri dishes or distribute
into sterile tubes.
© 2010 by Taylor and Francis Group, LLC
Malt Extract Agar, Half Strength 1001
Use: For the detection, isolation, and enumeration of yeasts and
molds. The addition of lactic acid suppresses bacterial growth.
Malt Extract Agar
Composition per liter:
Malt extract 30.0g
Agar 20.0g
Chlortetracycline solution 10.0mL
pH 5.5 ± 0.2 at 25°C
Chlortetracycline Solution:
Composition
per 10.0mL:
Chlortetracycline 0.04mg
Preparation of Chlortetracycline Solution: Add chlortetracy-
cline to distilled/deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.
Preparation of Medium: Add components, except chlortetracy-
cline solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile chlortetracycline solution. Mix thoroughly. Pour into sterile
Petri dishes in 20.0mL volumes.
Use: For the cultivation of yeasts and filamentous fungi (molds) from
cosmetics.
Malt Extract Agar
Composition per liter:
Malt extract 20.0g
Agar 15.0g
Peptone 5.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Candida melibiosica,
Cryptococcus curvatus, Kluyveromyces species, Metschnikowia
hawaiiensis, Rhodosporidium paludigenum, Saccharomyces cerevi-
siae, Saccharomycodes ludwigii, Stephanoascus species, and Trichos-
poron nigrescens.
Malt Extract Agar
(ATCC Medium 109)
Composition per liter:
Agar 15.0g
Maltose 12.75g
Dextrin 2.75g
Glycerol 2.35g

Pancreatic digest of gelatin 0.78g
pH 4.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–118°C. Do not overheat or agar will not harden.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of yeasts, molds, and Fla-
vobacterium lucecoloratum.
Malt Extract Agar
(MEA)
Composition per liter:
Glucose 20.0g
Malt extract 20.0g
Agar 15.0g
Peptone 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Arthrinium phaeospermum, Aspergillus
fumigatus, Aspergillus clavatus, Penicillium verruculosum, and Peni-
cillium spinulosum.
Malt Extract Agar
(BAM M93)
Composition
per liter:
Malt extract 30.0g

Agar 20.0g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and identification of heat-resistant
filamentous fungi (molds) from foods.
Malt Extract Agar, Blakeslee’s
Composition per liter:
Malt extract 20.0g
Glucose 20.0g
Agar 20.0g
Peptone 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of a variety of yeasts, includ-
ing Candida versatilis, Cryptococcus elinovii, Kluyveromyces marxi-
anus, Pichia species, Reniforma strues, Rhodotorula mucilaginosa,
Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Sporobolo-
myces roseus, Trichosporon nigrescens, Yarrowia lipolytica, Zygosac-
charomyces rouxii, and numerous filamentous fungi.
Malt Extract Agar, Half Strength
(ATCC Medium 2418)
Composition per liter:
Agar 15.0g
Malt extract 10.0g
Peptone 2.5g

pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–118°C. Do not overheat or agar will not harden.
Pour into sterile Petri dishes or distribute into sterile tubes.
© 2010 by Taylor and Francis Group, LLC
1002 Malt Extract Agar for Yeasts and Molds
Use: For the cultivation of yeasts and molds.
Malt Extract Agar for Yeasts and Molds
(MEAYM)
(BAM M182)
Composition per liter:
Agar 20.0g
Glucose 20.0g
Malt extract 20.0g
Peptone 1.0g
pH 5.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and identification of heat-resistant
filamentous fungi (molds) from foods. Recommended for identifica-
tion of Aspergillus spp. and Penicillium spp.
Malt Extract Broth
Composition per liter:
Malt extract 6.0g
Glucose 6.0g
Maltose 1.8g

Yeast extract 1.2g
pH 4.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not over-
heat—this results in darkening of the broth.
Use: For the isolation, cultivation, and enumeration of yeast and fila-
mentous fungi (mold).
Malt Extract Broth
Composition per liter:
Malt extract 17.0g
Mycological peptone 3.0g
pH 5.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 10 min at 15 psi pressure–115°C.
Use: For the cultivation of molds and yeasts, especially for sterility
testing.
Malt Extract Broth
Composition per liter:
Malt extract, purified solids 15.0g
pH 4.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–118°C. Do not over-
heat.
Use: For the cultivation of yeasts and molds.
Malt Extract Charcoal Medium
(DSMZ Medium 801)
Composition per liter:
Malt extract 30.0g
Agar 15.0g
Charcoal 3.0g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 10 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Boletus edulis, Xerocomus
badius DSM 4436, Antrodia serialis, and other filamentous fungi.
Malt Extract Glucose Agar
(DSMZ Medium 735)
Composition per liter:
Agar 15.0g
Malt extract 10.0g
Glucose 5.0g
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Morchella esculenta spp.,
Verpa digitaliformis, Disciotis venosa, Mitrophora semilibera, Gyro-
mitra spp., and Mitrophora semilibera.

Malt Extract HiVeg Agar Base
Composition per liter:
Malt extract 30.0g
Agar 15.0g
Plant peptone No. 4 5.0g
pH 5.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.4.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of fungi, especially yeasts.
Malt Extract HiVeg Agar Base, Modified
Composition per liter:
Agar 15.0g
Maltose 12.75g
Dextrin 2.75g
Plant peptone 0.78g
pH 6.0 ± 0.2 at 25°C
© 2010 by Taylor and Francis Group, LLC
Malt Yeast Extract 50% Glucose Agar (MY50G) 1003
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.

Use: For the cultivation of yeasts and molds.
Malt Extract HiVeg Broth Base
Composition per liter:
Malt extract 17.0g
Plant peptone No. 4 3.0g
pH 5.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.4.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of yeasts and molds.
Malt Extract Peptone Agar
Composition per liter:
Malt extract 30.0g
Agar 15.0g
Soy peptone 3.0g
pH 5.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.6.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 10 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Basidiobolus ranarum
and Sclerophoma pityophila.
Malt Extract Peptone Agar
Composition per liter:
Malt extract 30.0g
Agar 15.0g

Papaic digest of soybean meal 3.0g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Ascobolus immersus,
Aspergillus amstelodami, and Aspergillus clavatus.
Malt Extract Yeast Extract
40% Glucose Agar
(MY40G)
Composition per liter:
Glucose 400.0g
Agar 12.0g
Malt extract powder 12.0g
Yeast extract 3.0g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except glucose, to
550.0mL of distilled/deionized water. Mix thoroughly. Gently heat and
bring to boiling. Bring volume to 600.0mL with distilled/deionized wa-
ter. While the solution is still hot, add the glucose all at once while stir-
ring to avoid formation of lumps. Autoclave for 30 min at 0 psi
pressure–100°C.
Use: For the isolation and cultivation of osmotolerant microorganisms
from foods.
Malt and Peptone Medium
Composition per liter:
Agar 15.0g
Malt extract 10.0g
Peptone 5.0g

NaCl 1.0g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Adjust pH to 6.5. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and maintenance of Flavobacterium species.
Malt Peptone Yeast Extract Agar
See: MPY Agar
Malt Yeast Agar
Composition per liter:
Agar 20.0g
Glucose 10.0g
Peptone 5.0g
Malt extract 3.0g
Yeast extract 3.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Arthrobacter viscosus.
Malt Yeast Extract 50% Glucose Agar (MY50G)
(ATCC Medium 2093)
Composition per liter:
Glucose 500.0g
Agar 10.0g
Malt extract 10.0g
Yeast extract 2.5g
pH 5.5 ± 0.2 at 25°C

Preparation of Medium: Add agar, yeast extract, and malt extract
to distilled/deionized water and bring volume to 500mL. Mix thor-
oughly. Gently heat while stirring until boiling. Slowly add glucose
while stirring to avoid lumps. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Do not overheat or agar will not
harden. Pour into sterile Petri dishes or leave in tubes. Note: This agar
hardens very slowly.
Use: For the cultivation of yeasts and molds.
© 2010 by Taylor and Francis Group, LLC
1004 Malt 2% Yeast Extract Agar
Malt 2% Yeast Extract Agar
(MYA2)
Composition per liter:
Agar 20.0g
Malt extract 20.0g
Yeast extract 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Actinomucor elegans, Actinospora mega-
lospora, Agaricus bisporus, Ceratocystis perfecta, Ceratocystis cana, Cer-
atocystis seticollis, Chaetomium trilaterale, Chaetomium indicum,
Chaetomium seminudum, Chaetomium piluliferum, Cirrenalia macro-
cephala, Kluyveromyces species, Lepista inversa, Torula dematia, Tricho-
derma pseudokoningii, and other fungi.
Malt 4% Yeast Extract Agar
(MYA4)
Composition per liter:
Malt extract 40.0g

Agar 20.0g
Yeast extract 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Arthrobotrys arthrobotryoides, Ascospha-
era apis, Bettsia alvei, Ceratocystiopsis minuta, Chaetomium spino-
sum, Chaetomium piluliferum, Ciboriopsis simulata, Dactylella
minuta, Dactylella rhombospora, Dactylella lysipaga, Eriopeziza cae-
sia, Europhium clavigerum, Issatchenkia orientalis, Moniliella suave-
olens, and other fungi.
Malt 4% Yeast Extract Agar with Lupine Stems
(MYA4 with Lupine Stems)
Composition per liter:
Malt extract 40.0g
Agar 20.0g
Yeast extract 1.0g
Lupine stems variable
Preparation of Medium: Add components, except lupine stems, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes.
Cut lupine stems into 8.0cm-long pieces. Add 2–3 lupine stems per
tube. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For the cultivation of Ceratocystiopsis minuta-bicolor, Ceratocysti-
opsis retusi, Ceratocystis pilifera, Ceratocystis olivaceapini, Ceratocystis
multiannulata, Ceratocystis nigra, Ceratocystis olivacea, Ceratocystis
tremuloaurea, Chaetomium indicum, and Chaetospermum chaetosporum.
Malt 8% Yeast Extract Agar

(MYA8)
Composition per liter:
Malt extract 80.0g
Agar 20.0g
Yeast extract 1.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Thielavia hyalocarpa.
Maltea
Composition per liter:
Maltea 40.0g
Agar 22.0g
Yeast extract 3.0g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Leucogyrophana mollusca.
Maltose Peptone Yeast Extract Agar
See: MPY Agar
Maltose Peptone Yeast Extract Broth
See: MPY Broth
Maltose Peptone Yeast Extract Medium
See:
MP
Y Agar
Manganese Acetate Agar
Composition per liter:

Agar, highly purified 10.0g
Manganous acetate 0.1g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add manganous acetate to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
7.0. Add agar. Steam the medium to dissolve agar. Distribute into
screw-capped tubes or bottles. Autoclave for 15 min at 15 psi pressure–
121°C. Allow tubes or bottles to cool in a slanted position.
Use: For the cultivation of manganese-oxidizing bacteria.
Manganese Agar No. 1
(Mn Agar No. 1)
Composition per liter:
Agar 10.0g
MnCO
3
2.0g
Beef extract 1.0g
Fe(NH
4
)
2
(SO
4
)
2
0.15g
Sodium citrate 0.15g
Yeast extract 0.075g
Cyanocobalamin solution 10.0mL
Cyanocobalamin Solution:

Composition
per 10.0mL:
Cyanocobalamin 0.005mg
Preparation of Cyanocobalamin Solution: Add cyanocobala-
min to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except cyanocobala-
min, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 10.0mL of the sterile cyanocobalamin solu-
© 2010 by Taylor and Francis Group, LLC