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-
SPECIFIC PATHOGEN FREE
PROGRAMME
- (SPF) –
INTERNAL INSTITUTE DOCUMENT – DESIGNED BY THANH VU
NATIONAL CENTER FOR MARINE SEED RODUCTION
IN CENTRAL VIETNAM
RESEARCH INSTITUTE FOR AQUACULTURE NO.3
WHITE LEG SHRIMP
HATCHERY PRODUCTION TECHNOLOGY









I/ The pre-spawning process












!
"#

#$
!
I/ The pre-spawning process
%!%!

&


'()*+,, !

Employees of shrimp broodstock are produced in
Vietnam have been imported from Hawaii that have free
5 pathogens (MBV, IHHNV, TSV, WSSV, YHV).

When using domescated shrimp, it essenal to obtain
adequate background informaon on the origin of the
stocks and their past performance.
Shrimp broodtock’s tanks
I/ The pre-spawning process
%!+/"

0

!

"
!)



"
!/
1! #

!
I/ The pre-spawning process
%!+/"'!.

"
!

23
$
!

'!!
!.

3
/45!
Basic laboratory facilities
I/ The pre-spawning process
%!-

6
7
!
!


'
.
!
6#8
"!
97
!

!)

:!
I/ The pre-spawning process
%!;

$
!

6
3
3
!

"
'
.!



!

%,%+%+%;
<'
.
!
I/ The pre-spawning process
%!;'!.

Preferably, the maturation room should have
round tanks that are dark-coloured, smooth-
sided, and of approximately 5 m diameter.

The broodstock should be held with flow-
through (new and/or recycled) water exchange
of a total of 250-300% per day and a
continuous, but not too vigorous air supply,

Water depth is generally around 0.5-0.7 m,
Maturation tanks shape

The shrimp are stocked at a rate of around 6-8 shrimp per sq. m. bottom
surface area with a male to female ration of 1-1.5:1. Thus, a 5 m diameter tank
can accommodate 60-80 females and 60-100 males.

Water temperatures are usually controlled to be maintained in the range of
28-29
o
C, with a salinity of 30-35 ppt and pH of 8.0-8.2.

The feed preparation area should be adjacent to, but separated from, the
maturation room

I/ The pre-spawning process
%!;'!.


'.!

"'.
'/=/+,
.!


P.vannamei 
>?"!
-
)$
%>"!

%
%!@%!(3!

'"$.
"'+@
+A

4.
$"!
I/ The pre-spawning process
%!@ 




!

B
!

)3
')&&:=C&=/=.3
!

-,,@?
'.
D
!
'%,,-,./=/'@,%,,
%-.

The spawning tank
I/ The pre-spawning process
%!@ '!.

"
0=
$
E%F!
/"
+?+G

4-,@,
!

*6

'@%,.!


!
3!93

!

Broodstock with stage IV ovaries
I/ The pre-spawning process
%!@ '!.

8'
.!B

!
!
)
!
)
$'.


*
!
"%,,,,,%;,
,,,-,-@%@,,,,+,,,,,;,;@!


Using net to capture gravid female
I/ The pre-spawning process
%!@ '!.

!
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#'!.!

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!
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80=$
!

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II/ The post-spawning process
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
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'-@.


(
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6.!

7
'+?-+

4.!

$


"!

The hatchery and reservoir tank

II/ The post-spawning process
+!-!

/=/'+,8%@.!
+!;!B

&;D',!,@,!%
.$
/=/'@,%,,.%-.
!
+!@! :

:!

!
!)
IA,H!
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II/ The post-spawning process
+!@! :'!.


!
+!>!&:

&
!
+,,,,J;,,,,8

!

"
'-,.
"!

II/ The post-spawning process
+!A!:

%@,,,J-,,,,8
!
%,%@
$3!
7+?
-,

4%?+@

4! 
-+-@!
0
!
)
$
!
!

Transportation of Nauplii
II/ The post-spawning process
+!?!2

2"


)
K!

*
!

"3
!

2"!
+!G!2

&!'9
2L.'L.

*
!

II/ The post-spawning process
+!%,!2

!

"!

!
3!
)%,,+@,8'%,,
,,,J+@,,,,.


B"
B"7!/
"8
!
+?-+

4-,
!&?!!
(7"
!

II/ The post-spawning process
+!%,!2'!.

B"'!.
&
"
"


*

!/

!
M+N
%+#
!


!

II/ The post-spawning process
+!%,!2'!.

:
""
!9K"

!
B
"
"!
%,@,F
%,,+,,F+,,-,,F
!"
K!
97"
"
!

Algae - Chaetoceros spp
II/ The post-spawning process
+!%%!O


3
!

!)


!

!

(
!5
"!


3!

!
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