Đề tài kỹ thuật di truyền chuyển nạp gen vào lục lạp

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'%/?J'(9=o+‹'/76((/=A0'%&'745+1(+,-S*=CD74Mo@*`BM'{'%7L(q'%.5
+c'.@5'%+‹'/76((L'%'%/RS?'//U(.@5'%.N;'%+*?G
L'%'%/R+1(+,-M*'%+,?'/?B=9'%>1'%Cu.3?R..@5'%(L'%.T((e?./?R'%?P'%(EA

.@~'%.,5@*(T(%?P'%(EA(EAK/T'%7?@=SF'VMF7?K/=Œ'F(L'.@q'%74K/T'%./=P(
>?R.SE=F>?R.(r•3?2'Cw?(T(%&'+?&'<=*'C2'<=*'%/p-+4M.{'%'{'%S=V.(EA
.@~'%(/=A0'%&'(PC€'/'?.@5%&'745+1(+,-.,5@*+,-./0<=*'%/p-o3*''%4A
74(PC€'/C,M7453*'CJM(/=A0'%&'.,5`'/3$.+4M.*'%/4M+Np'%`'/3$.
74%?eM'~'%C$CNO'%.@5'%(EA(/=AJ'%&'M…/D*-@5.&?'745+1(+,-C0.,5@*
(EA(/=A0'%&'%?4=(/V.>?'/>NŽ'%F7?.*M?'SGL'%'%/R%&'+1(+,-C…74C*'%
CNp(9'%>1'%.@5'%'%4'/>Np(-/E'.I.,5@*7s(f?'74-@5.&?'.T?.w/p-.@5'%
-/h'%74C?B=.@€3R'/(/5'%NO?74C$'%78.G(L'%'%/R(/=A0'',-%&'+1(+,-(D
`BM'{'%@V..5+c'74(D'/?B=N=C?0M'w?.@$?/;'S57c?(/=A0'%&'745'/E'.2
'45'/NS*=
- Có kiểu di truyền mẹ( meternal inheritance)Cu(C?0M>?.@=AB'%&'(/=A0'
./&5K?0=>?.@=AB'MQo/_=/2../g=(a78.38.(*5F'%5,?.@lYSPd.+54?'/N
(D+?'/+{'%F+y*FC8=4+*'•o.@NO'%/p-(/=A0'%&'745'/E'F(D./0(DS6
%?*5-/V'o(T(CP?.Np'%K/T(G/ON=C?0M'4AM4.l'{MYZZn(T('/4
K/5*/U(C…K/=A2'(T57?R('%{'(/u'S6+*'.@=AB'()*(T(%&'(/=A0'',-
3b'%(L'%'%/R(/=A0'',-%&'745+1(+,-./6(78.G
- Mức độ biểu hiện gen chuyển nạp cao.@5'%Y.2'45(D/*'%.@{M+1(+,-F
MH?+1(+,-(/9K/5e'%Yii3e'S*5()*3$-+*S`>F74'/N78A(DK/5e'%
YiGiii3$%&'-+*S`>x.2345X*+5%*FYZZ:]G/d'/7m78AM4M9(C$3?0=
/?R'()*%&'(/=A0'',-@V.(*5K/5e'%kŠ%&'•Wo(T(>h'%K/5*?.EA
(/=AR'',-%&'X}?>5@57&.*+GFYZZZ]FkŠ%&'#.G4M+Np'%-@5.&?'M1(`J=
(D./0+J'C2'
kiŠ.@J'.w'%SP-@5.&?'/h*.*'o.@NO'%/p-(EA(4(/=*(/=A0'',%&'+,-
./0Xƒ&.*+GF\iiY]G_'CEAF•=@3S74C~'%.T(%?eX\iin]C…(/=A0'./4'/
(L'%%&'jY-\†745(EA(4(/=*FM9(3?0=/?R'-@5.&?'-\†+4†iŠG/N
78AF(L'%'%/R(/=A0'%&'+1(+,-(/5-/|-S*'S=V.@*CNp((T(+5,?-@5.&?'
.T?.w/p-(D%?T.@€.l./6(78.G
- Vị trí gắn đặt hiệu( Site-specic integraon)e'//No'%()*/?R=9'%7€.@d
.@J'(63?0=/?R'()*%&'(/=A0'',-•K/L'%(D/?R=9'%7€.@do-+*S`>>5
%&'v(//p-7457€.@dCu./?R=./L'%<=*/?R'.Np'%.T?.w/p-.N;'%

>~'%X/5M5+5%5=S@&(5M3?3*`5'&7&'.]GT(>h'%(/=A0'%&'+1(+,-./NO'%
C~'%'/V.X/5M5-+*SM?(]G}6C~'%'/V../NO'%'/8'CNp(<=*S6(/U'+U(
'%/?JM'%u.GG‘.@NO'%/p-(/=A0'%&'745'/E'FS6v(//p-'%‰='/?J'()*
%&'(/=A0'',-o'/?’MSs(./0./NO'%>‰'C2'S6/m'/./4'/(T(M6(3?0=
/?R'K/T('/*=F(T(>h'%(/=AR'%&'./NO'%M*'%v'/C~'%/p-/*A>€/p-
'/8'CNp(<=*S6.6./1-/E'S/5u(+*?/^=v'/G/N78AS63?0=/?R'%&'
C~'%'/V.o(T(>h'%(/=A0'',-%&'+1(+,-G%Np(+,?o.@NO'%/p-(/=A0'
%&'745'/E'FS63?0=/?R'%&'(/=A0'(DS63?2'./?J'(*5G
- Biểu hiện đa gen( Mulple gene expression):(/=A0'%&'745+1(+,-K/L'%%/?
'/8'/?R'.Np'%%&'?M+u'%GS6?M+u'%()*%&'(/=A0'',-./NO'%+4M
%?eM/5u(+U*.@lS63?0=/?R'%&'(/=A0'',-745'/E'.2345G
- Độc *nh protein của gen chuyển:(D./0%?eM./?0=3o?S6-/E''%{'()*+1(
+,-G‘.@NO'%/p-(/=A0'%&'745'/E'F-@5.&?'CNp(v(/+‚Ao.2345(/V.F
C?B='4A(D./0%EA@*e'//No'%C*/Nc'%Go+1(+,-F(DS6/m'/./4'/'P?
+?&'K2.>?S=+“>&35'>GC?B='4A+4M(/5+1(+,-+4';?+”.No'%Se'S=V.(T(
+5,?7s(f?'F>Np(-/ŒMFK/T'%./0FK/T'%'%=AR'.T?.w/p-74'/N78A(EA
(/=A0'%&'+1(+,-CNp(f&M'/N+4'/4MTAS?'//U(.N;'%+*?G
các phương pháp chuyển gen vào lục lạp:
/N(/y'%.*C…3?2.F(V=.,5()*+1(+,-(DM4'%CL?(/d'/(;(/278.+”C…
+4M78.(e'(/5S6(/=A0'',-745+1(+,-Gm78AF7?@=S747?K/=Œ'@V.
K/D./EM'/8-745+1(+,-G
{MYZnkF&#+5(K74C~'%.T(%?e3T5(T5+_'C_=`J'7B(/=A0'%&'+1(
+,-745ML.2345./=P(+T3b'%-/N;'%-/T-%?T'`2-./L'%<=*7?K/=Œ'
%@53*(.&@@?=M.=M&ƒ*(?&'SGT(.T(%?eC…'/8'CNp(YSP>h'%(/=A0'%&'
X(/+5@*M-/&'?(5+*(&.A+.@*'Sƒ&@*S&]GW/E'v(/}5=./&@'3+5.(/5./VA
%&'fEM'/8-7453$%&'+1(+,-(EA./=P(+TK/L'%w'C€'/743B'7^'%G
?R''*AF(T('/4K/5*/U('%/?J'(9=74-/T..@?0''/?B=-/N;'%-/T-
(/=A0'%&'745+(=a+,-3*5%~M-/N;'%-/T-3s'%&'X3?5+?S`(S]F>='%/p-
.2'45.@_'3b'%-5+A&./A+&'&%+A(5+XW•]F-/N;'%-/T-7?`JMXSI>1'%
M?(@5-?-&t&C–`JM745+1(+,-G

- GH+IJIKL+M
W/N;'%-/T-3s'%&'CNp(%U?7c?'/?B=./=8.'%^'/N3?5+?S`(F
35M3*@>M&'.GEA+4-/N;'%-/T-(/=A0'%&'.@6(`2-745(EA.@~'%GK—
./=8.3?5+?S`(>5˜5/'}.*'ƒ5@>oC,?/U(5@'&++-/T.M?'/GEA+4-/N;'%
-/T-@T./?R=<=e>='%C0(/=A0'+4745.2345(EA.@~'%X*5&.*+GF
YZZi]G?R''*A(D\+5,?./?2.3€3s'%&'K/T('/*=./?2.3€3*'C_=SI>1'%
+43$-/8'(='%(V-'{'%+Np'%%?P'%'/NK/Œ=Sy'%F%EA'w3b'%YK?M+I*
.J'./N;'%-/EM#?5+?S`(W}jYiiF=-5'.5M-*'AFg?+M?'.5'%F••./?2.3€
./9\/5,.C$'%./&5'%=AJ'.s(-/D'%C?R'F-/)+J'(T(/,.74'%(6(
M€'X/@?S.5=&.*+GFYZnn]7c?./=8.'%^™j(5*.&>%5+>-*@`(+&S]G/4'/
(L'%C_=`J'.@5'%Kz./=8.(/=A0'%&'+1(+,-CNp(#5A=.5'74C~'%.T(%?e
XYZnn]./g=(a/?R'.@J'CP?.Np'%.e5f*'/3b'%-/N;'%-/T-3s'%&'G'{M
YZZiF}7*374C~'%.T(%?eC…'/8'CNp((T(>h'%./=P(+T(/=A0'%&'Y[}
@?35S5MK/T'%\+5,?K/T'%S?'/+4S-&(`'5MA(?'74.@& 5MA(?'GG(/5
C2''*AF 3s'%&'745+1(+,-C…CNp(`2'/4'/./4'/(L'%o./=P(+TF(4
(/=*F(4vMFK/5*?.EAF(T((EA/U(e?•GG
%=AJ'+”(/='%()* '4A+4SI>1'%T-+6(()*f='%K/d/&+?=MC–CŒA(T(
7?J'C,'(DKd(/./Nc('/rM*'%%&'M5'%M=P'X./NO'%+4*=F.='%S.&'F
`.*'?=M5@75+ƒ@*M]f=AJ'<=*(T(+c-.2345FMLC0fEM'/8-745%&'5M&
./6(78.
š=C?0M›'/Np(C?0M
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