KHẢO SÁT SỰ LÊN MEN SỮA CHUA TỪ SỮA ĐẬU NÀNH VỚI CHỦNG LACTOBACILLUS ACIDOPHILUS & BIFIDOBACTERIUM BIFIDUM

VIỆN CÔNG NGHỆ SINH HỌC VÀ THỰC PHẨM

BÁO CÁO TH CÔNG NGHỆ SINH HỌC THỰC PHẨM
KHẢO SÁT SỰ LÊN MEN SỮA CHUA TỪ
SỮA ĐẬU NÀNH VỚI CHỦNG
LACTOBACILLUS ACIDOPHILUS &
BIFIDOBACTERIUM BIFIDUM
GVHD: TS. ĐÀM SAO MAI
ThS. LƯU HUYỀN TRANG
NHÓM: Chiều thứ 6 – Tổ 3
Thành Phố Hồ Chí Minh, Tháng 4 Năm 2011
DANH SÁCH SINH VIÊN
STT HỌ TÊN MSSV PHÂN CÔNG CÔNG VIỆC
   
  !"# $ %&
'  ()*+ $'
(,-.%/%
0%,
1 2/ 
034,
%567348%9
: ;<=+4 : >+&#+.
MỤC LỤC
'??@4+ABC4D????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????:
?7ABC4D?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
'?%58%9??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????'
1?7E*+-8F+8%9????????????????????????????????????????????????????????????????????????????????????????????????????????????????????1
TÀI LIỆU THAM KHẢO 18
I1. MỞ ĐẦU
1. Giới thiệu chung
G%98#4+4H&;I+%9+J+KL+*%MN4,BC46K,

O8+CP++MQH6J++RMH+S6K+Q &+OK+Q8J+TU?
G#4+4VMMWT/%0LR;2XO+/+Y+%."V"68OZ+NT/
Y[\(,4?G\],%2X6+.8\&#,O&&^+94
T8#4+4M_+ 8#4+4M`L4abY+c:T+4K
_41:Td`c16%c6+ELe_%&08S`c6:4+&LeLWN4c6$+ELe
T]LWN4f4+&c6$%f4+&LeT]c'6+4L&4c6+Eg.cO6
4O+8?
;OY;+h4B+J&i4+hOT9bLactobacilus AcidophilusO
Bifidobacterium BifidumBBB,8#4Mj<+MP+;48%Z8#4
+4Y6..6&&^.64P+EkMH%/lO+EO.O8
OY+/+8%98#4+4MW+C8mBQ(,4?P+EkMH%/lj\8I
T/+L,+/+%&&^8%9634=%O%&?no+P
8%9M+3RML\Mo+PB,+Pb8#4M?
2. Tổng quan tài liệu
2.1 Nguyên liệu đậu nành
n+CBT4=+p+4g2?n+C8F+T/+46MC
M+COSEBM+aO8q&<
5;M+C'LH%b
f(U;+Rr=;
f]+Rr
fq&,%+Rr
s/&&^+h4;Mb

%M+34"EM6C+J4MMh+/+4+&4T]
4ROYA,+"MS?4+&C4&&+%MMR5t:?:8uM]<
%8#4MVTS.MSMa6;B+E*+MaE6Mv%+h4
8%98#4+4?/+"4+&C44+&4++88V3/wB+h44+hO
T98q&<?
2.2 Các chủng vi khuẩn lên men
2.2.1. Lactobacillus Acidophilus

Lactobacillus acidophilus_L.acidophilus`;%L+6OT94&.6&;3+C
M]N6jM.[6+o%o++x+C@H+yF6+CTKBMQ
4+&4++6
%
t'z76E44+&?n"OT9&@_8`?(YwJ+4
M0+EBMaw6+*+C@+@C4R&g8+C]
Q4+&4++_T@+Ma%"{O|`_548O(6:`?

GI%"W+h4J+K&T9L. acidophilus8gE44g4++6&%g&};4
MT,]Q+S;+/+8OT]4P+?L. acidophilus+x8gE44+486
;~+CTK%"•MQ8#4_4+8`+/+;MQM.?#Q
+C+.M4T]&;%4+8wT]@8gE4;~?(wD&MC6I+%9
L08L. acidophilus +C@+C+#MS?
2.2.2. Bifidobacterium bifidum
Bifidobacterium bifidumH#+hO8OMQH+CP+?Bifidobacterium
T]&MH6T];Lq6p4&.6w3OYE&;6%YTTPLF
LH+?(T9Bifidobacterium8SO;MHS\%5€'6:?7*+CTKJ++R+/+
O8O+C;,SBC4?7CTKB+/+;MQO%5]
QB?
/+MH+h4OT9Bifidum8#4+4*%MH++/++E"6TP+P+
8IM/%J &+O+8R4?
n+h4CLY++9LUV%NP,OYS+C@J&<O
I+R8gEj%%*O+,.#4+],I+%9Y+
.4T]g4?
pH%+;E++/+%+A+Nf46E+MP+%%Y
,6DMV+4<+U%Y,?BYLL/T+CB
%034,6C%M+P+%H+/+TeeO%\M?
II2. VẬT LIỆU – PHƯƠNG PHÁP
1. Nguyên liệu
1.1. Các chủng vi khuẩn

faS+B,b|EVLH8%SQn;5=+s/+4%?572
f"Sb
 79L]Q"Sb%4]Q2•G_:‚`ƒE++/+%4
MOƒ%"%SO„ƒE%TqiƒM@H?
 7ESO„?
'
f…K8bMhK8+/+„MWM++ES1\,MH%N
1.2. Sữa đậu nành
aS+B,b8#4MW,(†8T]MQ_B8.34O%+h4
8%`?
2. Quy trình tiến hành
???
3. Phương pháp phân tích
3.1. Kiểm tra chỉ tiêu vi sinh
2]QT@42•G0%MW+9L8mM)4%?2ZM+%4WI
&W+/+aMH%%"
f
MR
f
?5*6Z\yaMHMWM+%4.
1
7EZ
…_f1`
B,8#4
4i:f

7
_:%*`
n@HO1f1:


7
EM
"%SOx
7ESr_(‚(`
s08MQr_‡‚O`
JV
f1
MR
f
+OM)4%+C+J4]Q6MLj34_I+,B
Z`?G4MCh\,MH%N1?
nR8ST9;+gE,BM)4V:-:?TR3\yMH%4WOgqD8S
,7ˆ‰?
MC6
b08ST9;+MRM+?
n

b8SM)4+C8ST9;+M++=;yaMH%4W?
vb&P++EOyM)4?
d
i
bMH%4W+C8ST9;+M++=;+/+M)4MR?
3.2. Kiểm tra chỉ tiêu hóa lý
 Phân tích protein:
n0Lj%.%/%Kjeldahl. Xem xem pp này có khác so với pp gốc ko, nếu
khác thì ghi ngắn gọn phần khác biệt, ko thì thôi.
Tính toán kết quả:
(wP,+CR88OYZFB48u8q&<+]J+&YM"M@P
/b
MC6

b@P+4Š5_|`&iM@+9ZF_ZT+CM; 5GŠ1+N
BOvb:|`?
sb@P+4Š5_|`&iM@+9ZI+_Z+CM;5GŠ1+N;84
TMWE%%<5'`?
baMHM.&&4Š5&i+9MH_?`?
,8S%4W?
|%]_r`trN%gT
Tb,8S3M0Vr84%6OYZ8#4TQt:6'
 Phân tích acid:
:
{i%.%/%+9MH4+&-L48bC8.34%%_&N`
Tính kết quả:
54+&PLj4+&4++M+PLj+]J+b
MC6
(

_|`b@P+&&4Š5&i+9Zq
(

_|`b@P+&&4Š5&i+9ZF
?bTS+h4&4+&4++
baMH4Š5&i+9Z
b,8S+@M0O‚|
(_|`b@P+Z&iL4M
 Phân tích lipid:
Gq&<%.%.%/%Adam – Rose cải biênb
Tính kết quả:
MCb
b=+e‹%&_`
Œb=+e_`

pb=Zq_`
Tiến hành:
7OLwF;b
• 2Zb:f_MW+"8m+"`
• {&++a44+_+a

:b44+:|`b
• „b
• 7Ab=
|*+MF+Tu684F+;&O+S+iF+;?n@B'%*6Lw8u+4
Y%b
 |Y%&YY%44+N44%O+/+%T/+
 |Y%BN44+ELeOZH8S+ET/+
/+EY%6LUY%44+o+#EM@M%%%TRh4L\4+&?
7BOLwF&U46F+;aM@B:%*6+/++ET%+E
Le8u/+4OFgS&YM/LwF;6M+&aOY%&&44+?
$
7@R%O+ehMW8ET]O+"8m?•q4LwF;6yOY
:O&aR+O+eh?n@L4.R\,MHQ684MC++Oh
8E:

7'%*?|E4M@OLw*9+MRHaM+"?
III3. KẾT QUẢ - THẢO LUẬN
•4TR3T8/8.LHO+/++ABO86C486&I4O+&&^O+/+Mo+
M@8D+h4+hOT9Bifidobacterium bifidum và Lactobacillus acidophilus6C8u
M+%MR8IT/+4T8q&<'aB,86+‡6++M@+RLR?
1. Chỉ tiêu vi sinh (CFU/ml)
Hệ số pha
loãng
Số lượng vi sinh

(Đĩa 1/Đĩa 2)
Thể tích sử
dụng
Ghi chú

f1
ŽŽŽŽŽŽŽŽ 6 2MHT9;+&Mo+

f:
ŽŽŽŽŽŽŽŽ 6 2MHT9;+&Mo+

f$
''‚1: 6

f
$‚' 6

f
'‚ 6

f
'1‚$ 6

f
‚ 6

f
‚ 6
0+H :'‚1
n4LO%<<+

GSRL6Z_:€€'`
!eMHRL+h4+hB']Q+C8IT/+4?nSOYB,8#4&V4+C
MHRL+4EO8#4LNwE%E?•4MC64EM++C8I0MO8SRL
MSOYB,8?

Nguyên liệu Mật độ tế bào (CFU/ml)
G 61?

7+ 6?

7‡ 6:?
$
2. Chỉ tiêu hóa lý
2.1 Hàm lượng Nitơ tổng
@P+4Š56&iM@+9ZFTb:6:?
@P+4Š56&iM@+9ZI+Bb1$6?
r.%b
5]b
(YT,8S3M0Vr84%6Tt:?
s88/TR3b
Bảng 1:5%+h4+/+;8#4+4+i+hOT9T/+4OaB
,

Bảng 2b5%+h48%98#4+4OY+/+;BQ
G#4M+xB%034MW+CM+%6geO%w+4.
8OY8#4LNO8#4&V4?4+\aB,8q&<w(†8+CrM;'6‚
+N(4T,i'6‚?
7xL4+x+C@EM+8IT/+L,6%8#4
+4M+C8IOH•#4+/+;+i8q&<V+9OT9S+AT/+
4OB,?5%+h48#4+4ME%168OY8%9V8#4LN

O:6:OY8#4&V4?8S,BI+RT]%+C8I+B,+3/YO
C+x+4EM+M@+h48%9+Pj\%?B
"&ZY8I848S+C@&MT,%NP,_/C+6C4+E}`6&4
/+843/wI+,6&#4<}O+x+POwB"+
+h48%98#4+44;+4.8OY8%9•8MW+CoB
Q_E%6$'`?
s\OM@+JM+M@OH+h48%9w48uEM+]34O,+8
8/#48%98#4+4(4TO•8?G#4+4•8+C%YE%
?:6M+JUM+M@+h4;?]#%+46%
8#4M+N+J4+/+;4+&4RROY%+"MS6Mo+L,+J4
4+&4;4O46#4+&4*%&wO+h+S,  

++.@?(wO34TR388/4*4M+M@E+S++*DMR8#4
+4M+P%O/+&<+h4CMSOYQ8q&<?
2.2 Hàm lượng Lipid
R3b
=Z
G_`
=+e
P
’
_`
=+eO|%&
P_`
2Z 6 :6 :6
2Z 6 :6 :6
K|%&b
G P
’
P % Lipid TB (%)

2Z 6_` :6_` :6_` 6
6
2Z 6_` :6_` :6_` 6$

54‘G\],%&&^+.8\&#,++94T’’’6]&
&^+h48#4+4M_8#4+4M+`L4a6+ELe_%
08S`6:4+&LeLWN466$+ELeT]LWN4f4gc6$%f4gLeT]
?
5%&8%9I+E%.8OY%&0+h48%9
8#4+4MBQ?7C8IT/+L,+C@&+/+B"84b
aB,+CgEgJT/+4
•/wI+,64/+P,+4M++96"4<8%9&ZMR848S?
{I4OMaw4EM+6+C8IT/+L,EYO%&8%94
VB,8#4&V4MSOY4VB,8#4LNO8#4M?5%&
8#4&V4+4E%?$$8OY8#4LNO+4E%1?:8OYB,8#4M?
7C8IT/+L,M/T@8%94&aB,L4M?4MWLR6
8#4M+C%+46P+ELe6T8#4&V4+C+ELeE+4?

•*4wB4++6OT9T]8q&<%&B%&.MS0MOa
;8%9??
GOY+/+;8#4+4T/+BQ68#4+4M+C+ELeE%6
T]+J4+ELe484+8?5H;+2XM44HBLSO8J+TUjb
+RMHK+C+J4H+ELeLWN4E%O+86OY:4%M
6+.L,;+?
n8#4+46OY H +Ew&&^.I8#4+4P+ELe_|‡f†4
•t|fˆ•`6M"HI4+=S+#QT]@K8#4+4]Q
o+#QT]+CTKE%<+/+8%98#4+4M+V8#4&4?
2.3 Hàm lượng acid hữu cơ
R3b
2ZI+V

1
_` 2ZFV
2
_`
| 6'
6| 61
Lw 6':
54+&PLj4+&4++M+PLj+]J+b

•/wB4++bMQ4+88uM+OT9+@C44+&4+++4
8#4?7/+4+&%5+h48#4+4O;+R8I%/@+h4OT9HMH+I+%96
;8%9%<MCO4N34=O,+C%%wBi6OMo++
#8%9B4++?n/+*DE&4+O4+4&&?
7  '  8  %9  86  +‡6  ++  +i  8q  &<  +h  O  8  O L.Acidophilus O
Bifidobacterium. (wO4+&#+.w++C8I+B,+T]Y?B
OZ+C8IT/+L,UO4+&#+.6+4EMSOYB,8#4
&V46E%EMSOY8#4LN?a;8IT/+L,+C@&T)B+h4'C8q
&<B,86+‡6++T/+46MT,BT/+46MQ
4+8B,\Y3/wB?
G#4+4M+C.OE=?B6,4+C+/+W,8#4+4M
T]MQMW"%OQ?
3. pH sản phẩm
G%9+C%5t161-161?p/jT%53M16-16$MSOY8
%98#4+4
'
4. Cấu trúc – màu sắc sản phẩm
2bF6.O
2ib.8#4M
(b+4Le
7E*+b+F+6Mo+68/6?

1
IV4. KẾT LUẬN
•43/wI+8%94E8%9E.i8#4+46M]<S6+E
*+;TS6T]Q6T]E+Y+}C+8%9T/+]}
# /+ &< +h4 %L+ MS OY 8J+ TU  # . /+ %J+ ;%? (wO6+.+R
/+&<+h4%L+8uR%<+M+B+JOP+AA.?
.46;8%9+J4%L+8uM+4MQ?B+/+8%9L0
8%L+M4+RR&TKJ&<+h4C+4.I+R?7/+8
%9+J4%L+O%L+8M4+KO8uR%<+K?(wO6+/+8%9
+J4%L+8uM4&;.?
“(, 46 J+ K O Y+ S   O 8 O  E ? (w O6%L++C@
P+%.M@y+J+K &+++.@?L+8uR%<+%/@\#
8%9CMW+RR+/+8%98#4?
B6 %L+ OZ +C  K M@ %/ @ B +/+ 8 %9 T/+ 8#4? { MC6 8I
+]O%/@+h4+/+8%9%L+ &IL/8I +]+h4 +/+
8%9
%L+?
Tài liệu tham khảo phải viết ngay dưới phần kết luận.
Nhìn chung format bài ổn, đã biết cách viết 1 bài báo. Chỉ có một số điểm chưa đạt lắm,
nhưng thôi do bạn chưa có kinh nghiệm nên tôi cũng ko đòi hỏi nhiều.
Nếu có thể giải thích thêm dựa vào các cơ sở từ các nghiên cứu trước được thì càng tốt
Sửa lại chút là có thể in ra được.
:
PHỤ LỤC
1. Sơ lược về đậu nành
GOY+/+aM;I+OT/+6%VM++A8SM
M;+4.•?B+JD=+OT4=+MWT•
MO4NK+h4M8J+TU;+6
@+86TS+R=68J+TU,g.6+/+
,+J4M;WT6KV4+/+;_

O*6R,6q+6I+}`6y#,+O,+M
L,@MQ6K+Q+J+K+h4, &+?(
/?6+.34T@4I+%9O&+%954‘
_ˆ{>`M44TR+/k:4M;Mj6
T9%KjOYP+ELeLWN4O+8+C@
+.M4l?R+/.I+xM+M44V+.34R+/•R+h4
>•S+_d5474”4Od57”`?
no+L,M+C+J44+&4;4O46M"#4+&4*%
&wO+h+S,  ++.@?Ld8p46+/+%J8C4IB
+h4M;M*%+.@+8|{|6MQR6VMC
+.+/+L,O;+O@MQ?n;M+N+CP+T/++8J+TU
;+b^/6R/%MSOY#L,"+4R/%6K8J+
TU+h4+/+;+/+.@?
2. Sữa đậu nành – nguyên liệu sản tạo ra sản phẩm sữa chua đậu nành
G#4MM++RLRV;M+C+J4%SE+/+
;%VI+O6O+C/&&^T]Te8#4LN?wVC+
MH&&^6RS8#4MQgB6+*4+C@E%<
MR:r%+C?%&&^+h48#4M+C
M@.IOY8#4LN?G#4M+C%+4Lj8#4LN6
P+4g.8#4LN?G#4M+CM@T]+C4+86+C@
4R8#4LN+#QL& LM4L<&T]E%4+8?
G#4M+x+J4P+ELeLWN4.8#4LN6+C@+C+
$
;+.?CT]+C+486H%+h48#4LN+C@;484OK8gE+E
+.@?
7/+%&&^M+34"8#4Mb
 Protein (chất đạm)
M+E%O+.@&Y&;+/+4+&46BO,g"&IO/;+/+
0+J++.@6%+P+h4+/+T/@*%+.@+S;+/+L, 
T9?naQC+x%+h4+/+O+/+HRS_`E34=

3/w+@C4+h4+.@?
+N44O+/+;MH8D+h4+.@44BC4J+K6O+@
]g6;MH+h46;MH+h4WLH???
V#M@MWBO/%M%0346aM;8#4
MB,/M++*DE\Mo+P8%98#4+4+h4+*4?
G#4MB,+CM;T/+4b$?1‚O+CMMh+/+4+&4T]
4ROY+"MS6M/34"?
 Lipid (chất béo)
7ELe*%+.@E%+/+O4Mo+L,+/+O44&O4>6{6„6
OMCO4NO3/wM]/IB?
|%&+xa++E%K++.@?
5+ELe8#4MB,EE%_'?‚`6E%.8OY+/+
B,8#4+4T/+b8#4LN68#4&V46}
7ELeT]MCO4N3/wB8%98#4+4M?
 Các vitamin, chất khoáng và vi khoáng: +xH%34=Mo+
P8%9?

TÀI LIỆU THAM KHẢO
? (,+],8=+I+%96Giáo trình vi sinh vật học đại cương6Qn57]
,%?572?
? (,+],8=+I+%96Giáo trình thực hành vi sinh thực phẩm6Qn5
7],%?572?
'? (,+],8=+I+%96Giáo trình hoá phân tích6Qn57],%
?572?
1? (,+],8=+I+%96Giáo trình thực hành phân tích thực phẩm6Q
n57],%?572?
7/+4‡Lb
%b‚‚‡‡‡?&&?O‚†%4‚&484‚:‚?48%g
%b‚‚‡‡‡?L4+4?+‚„s++T&‚%+‚'''‚|4+L4+8
%b‚‚‡‡‡?44?+4‚•8+4‚4+&%8?

%b‚‚‡‡‡???O‚&%8‚&†‚44‚?
%b‚‚O?‡T%&4?‚‡T‚Gr„rssr>ˆ4Žr71rr„rs>r>{Žr7'r>
%b‚‚‡‡‡?L844?+?O‚{†4?48%g’t>+{4–>+”{t'1
%b‚‚‡‡‡?&&?+?O‚8‚4ff&f&ffO4f4+ffOf8f+4f84
%b‚‚O?‡T%&4?‚‡T‚Gr„rssr>ˆ4Ž+4
%b‚‚‡‡‡?L?4+?T‚+4‚8‚L4+4‚|4+L4+8Ž4+&%8?
%b‚‚‡‡‡?4&+?+‚4+L4+8f4+&%8‚
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