(Pangasianodon hypophthalmus)
2014
Pangasianodon hypophthalmus)
2014
1
Pangasianodon hypophthalmus)
1
1
)
nuôi. 1
; (3)
(4)
38±0,20
,43±2,23
p<0,05). N
k
1. GII THIU
nuôi
2
dan
Edwardsiella ictaluri
-glucans, vitamin C ha
tra. V,
Pangasianodon hypophthalmus
2. U
2.1.
2.1.1. B trí thí nghim
2
, t
.sang bóng.
2.1:
N
1-2
3-4
5-6
7-8
9-10
11-12
0
0
0
0
0
0
150
0
150
0
150
0
150
150
150
150
150
150
250
0
250
0
250
0
2.1.2.
Trong s
M
-
tro
tro-15. máu
3
complement, immunoglobulin (IG).
Qui trình thu và
2.2. n E. ictaluri
2.2.1. Chun b dung dch vi khun
Edwardsiella ictaluri
ch
n . Sau
9
CFU/mL. V: 10
6
CFU/mL v
lê 28°C sau 48
x
2.2.2. B trí thí nghim
kh.
các gia ;
sáng
2 - 3 ngày t
6
2.2.3. Thu mu
-.
2.3.
trong 500 µL L-15 trung hòa. Sau
-15 trung hòa
xong
-
Ly tâm 6 phút
(6.000 vòng)
4
-15 trung hòa và ly tâm
trên. Thêm 150 µL NBT
100, 125 và 150 µL NBT
0,6 g trong 5 phút, cho tuýp methanol, KOH (2M) và N-
N-dimethylformamide tâm 5 phút ng 550 nm.
(complement) theo Sunyer and Tort (1995).
p
3.
3.1.
3.1.1. NBT (mg/ml)
3.1 (mg/ml)
0,09±0,04
a
0,11±0,03
a
0,14±0,09
a
0,13±0,20
a
0,22±0,17
a
150
0,09±0,03
a
0,14±0,02
ab
0,14±0,08
a
0,30±0,18
b
0,38±0,20
b
150
0,09±0,03
a
0,11±0,03
ab
0,17±0,07
a
0,14±0,09
a
0,15±0,10
a
250
0,09±0,03
a
0,16±0,07
b
0,17±0,12
a
0,37±0,21
b
0,36±0,16
b
nên
cao
0,16±0,07 mg/ml c
05. H ca,
17±0,07
i nhau (p>0,05)
5
0,37±0,21
0,30±0,18
(p<0,05)
c
n
38±0,20 mg/ml
mg/kg l không
.ng et,
al (2013)
(Pangasianodon hypophthalmus
01%Theo
Sajid Maq
Cyprinus
carpio)
,
theo Logambalet (2000)
superoxide
5%.
3.1.2. IG (mg/ml)
kê 05
6
13,45±0,41 mg/ml.
9,95±1,52
a
10±2,05
a
11,1±1,48
a
11,78±1.93
a
10,93±2.63
ab
9,09±1,67
a
12,75±1,93
b
11,55±1,21
ab
13,07±2.20
a
12,43±2.23
b
10,86±2,9
a
11,98±3,49
b
10±2,18
c
12,26±2.22
a
10,63±2.70
a
10,86±2,9
a
13,45±1,74
b
12,35±2.02
bc
12,87±3.19
a
11,93±2.28
ab
mg/kg35±2,02 mg/ml
±2,18 mg/ml
c, n
13,07±2,20 mg/ml. Tuy nhi
43±2,23
10,63±2,70 mg/ml,
i
3.1.3. (U/ml)
H
82,05±3,18
a
90,27±4,68
a
92,51±2,12
a
92,54±0,54
a
93,92±1,02
a
88,82±1,45
a
126,45±7,75
b
117,32±9,94
b
147,83±7,34
b
213,09±1,32
c
84,26±2,77
a
126,45±1,63
b
135,44±7,27
b
205,30±1,11
c
193,01±10,74
b
83,33±4,00
a
128,97±1,29
b
123,83±5,62
b
234,83±7,86
d
200,03±2,74
bc
n
7
levamisol
150 mg/kg
213,09±1,32
Hang et. al (2013
(pangasianodon hypophthalmus() mg LPS/kg
c
3.1.4. (complement)
(Unit)
4,53±1,04
a
5,46±1,40
a
6,86±2,73
a
6,16±0,84
a
6,68±1,35
a
5,21±1,30
a
10,61±1,83
b
10,43±1,05
b
11,98±1,45
b
12,67±1,43
c
5,82±1,24
a
10,34±2,52
b
11,37±1,47
b
11,10±1,47
b
11,01±1,45
b
5,43±1,54
a
12,25±1,82
b
12,22±1,61
b
11,88±2,11
b
11,55±1,19
bc
N
không
± u 28
levamisole không
8
1,61 Unit.
3.2. E. ictaluri
không
,
. San gan
- ctv
).
sau khi gâym.5.
(%)
(%)
(%)
23,33±3,3
a
16,67±3,3
a
40,00±0,0
ab
63,33±3,3
b
60,00±10
c
50,00±5,7
b
43,00±3,3
bc
70,00±10
b
,05
, cng levamisole
ng 3.5
u tiên. T
05.
m
9
.
(2012) E. ictaluri (0,25x10
6
CFU/mL),
k
(2012
n E. ictaluri
5
CFU/mL
,
trê E. ictaluri
NBT (mg/ml)
(mg/ml)
0,08±0,04
a
0,07±0,03
a
0,09±0,04
a
0,53±0,16
b
0,12±0,02
a
0,20±0,07
a
0,10±0,05
a
0,51±0,18
b
,
(p>0,05). H
04 mg/ml. K
05. E.
ictaluri (mg/ml)7
10
13,47±0,54
a
13,67±1,04
a
14,94±1,26
a
15,8±1,17
b
16,28±2,78
ab
13,53±1,13
a
18,70±3,71
b
14,47±1,15
ab
71 mg/ml
28
n
17 mg/ml.
E. ictaluri
3.8
8
28
94,85±0,51
a
96,86±0,93
a
168,09±12,87
b
312,45±11,84
b
185,36±6,71
b
235,92±3,11
c
186,24±2,55
b
288,23±2,77
d
so mg/kg
(p<0,05)
E. ictaluri
11
ng 3.9
10,26±2,80
a
12,10±0,77
a
12,25±0,84
ab
14,94±1,19
b
12,64±0,60
b
12,87±0,78
a
14,98±092
c
14,36±0,80
b
mg/kg mg/kg
mg/kg
4. KT LU XUT
4.1.
150 mg/kg liên
E. ictaluri.
4.2.
Bui Thi Bich Hang, Sylvain Milla, Virginie Gillardin, Nguyen Thanh Phuong,
Patrick Kestemont (2013) In vivo effects ofEscherichia colilipopolysaccharide on
regulation of immune response and protein expression in striped catfish
(Pangasianodon hypophthalmus). Fish & Shellfish Immunology 34: 339 - 347
(Pangasianodon hypophthalmus)Edwardsiella ictaluri
12
Pangasianodon hypophthalmus
-154.
Ellis AE. Lysozyme activity. In: Stolen TC, Fletcher PD, Anderson
BS,Roberson BS, Muiswinkel WB, editors. Technique infish immunology.NewYork:
SOS Publications; 1990. p. 101 - 3.
Logambal, S.M., Venkatalakshmi, S. and Michel, D.R. 2000.
Immunostimulatory effectof leaf extract of Ocimum sanctumLinn. in Oroechromis
massambicus (Peters). Hydrobiologia, 430: 113-120.
Milla S, Mathieu C, Wang N, Lambet S, Nadzialek S, Kestemont P, et al.
Spleenimmune status is affected after acute handling stress but not regulated by cortisol
in Eurasian perch,Percafluviatilis. Fish Shellfish Immunol 2010;28:931 - 41
Tae Sung (2012).(Pangasianodon hypophthalmus)
Siwicki AK, Anderson DP. An easy spectrophotometric assay for determining
total protein and immunoglobulin levels infish sera: correlation tofish health.Tech Fish
Immunol 1993;3:23 - 30
Sunyer JO, Tort L. Natural haemolytic and bactericidal activities of sea bream
Sparus aurata serum are effected by the alternative complement pathway. Vet Immunol
Immunopathol 1995;45:333 - 45.
Sajid Maqsood, M.H. Samoon, Prabjeet Singh, 2009. Immunomodulatory and
Growth Promoting Effect of Dietary Levamisole in Cyprinus carpioFingerlings Against
the Challenge of Aeromonas hydrophila. Turkish Journal of Fisheries and Aquatic
Sciences 9: 111-120.
(Pangasianodon hypophthalmus). -142.