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ảnh hưởng của levamisole lên các chỉ tiêu sinh lý miễn dịch trên cá tra giống ( pangasianodon hypophthalmus)

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











(Pangasianodon hypophthalmus)













2014














Pangasianodon hypophthalmus)














2014

1

Pangasianodon hypophthalmus)


1

1





              
)           

nuôi. 1 
                
; (3)
               
 (4)


 
38±0,20
,43±2,23
p<0,05). N

 k
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          


 


 
 

 nuôi

2
 dan 

Edwardsiella ictaluri

 -glucans, vitamin C ha
 tra. V, 
Pangasianodon hypophthalmus 


2. U
2.1. 

2.1.1. B trí thí nghim
   

            
  
 2

, t
 
.sang bóng.
          

2.1: 
N
 
1-2
3-4
5-6
7-8
9-10
11-12

0
0
0
0
0
0

150
0
150
0
150
0

150

150
150
150
150
150

250
0
250
0
250
0

2.1.2. 
Trong s  
 M
 -
 tro
      
tro-15. máu 

3
  complement,  immunoglobulin (IG).
Qui trình thu và 
2.2. n E. ictaluri
2.2.1. Chun b dung dch vi khun
 Edwardsiella ictaluri 
ch  
     n    . Sau   


  
  
  
 

9
CFU/mL. V: 10
6
CFU/mL v
 


lê 28°C sau 48 

 x 
2.2.2. B trí thí nghim
 kh.
 các gia ;
  sáng
 
   2 - 3 ngày t          

               
6
  


2.2.3. Thu mu



 
-.
2.3.  
 trong 500 µL L-15 trung hòa. Sau
 -15 trung hòa

xong


-

Ly tâm 6 phút
(6.000 vòng)

4

-15 trung hòa và ly tâm
  
 trên. Thêm 150 µL NBT 
100, 125 và 150 µL NBT 
 
0,6 g trong 5 phút,  cho  tuýp methanol, KOH (2M) và N-
N-dimethylformamide tâm 5 phút ng 550 nm.

            
 (complement) theo Sunyer and Tort (1995). 
 p
3. 
3.1. 
3.1.1. NBT (mg/ml)



3.1 (mg/ml)







0,09±0,04
a

0,11±0,03
a
0,14±0,09
a
0,13±0,20
a
0,22±0,17
a
150 
0,09±0,03
a
0,14±0,02
ab
0,14±0,08
a
0,30±0,18
b

0,38±0,20
b
150 
0,09±0,03
a
0,11±0,03
ab
0,17±0,07
a
0,14±0,09
a
0,15±0,10
a
250 
0,09±0,03
a
0,16±0,07
b
0,17±0,12
a
0,37±0,21
b
0,36±0,16
b




 nên


cao

        
0,16±0,07 mg/ml c
05. H ca,
          
17±0,07 

   
i nhau (p>0,05)

5
              

0,37±0,21
0,30±0,18
 (p<0,05)
c
 
 n
38±0,20 mg/ml
 
mg/kg l  không



.ng et,
al (2013)            
(Pangasianodon hypophthalmus
        

01%Theo
Sajid Maq
               Cyprinus
carpio) 


,
    theo Logambalet (2000) 
                
superoxide
5%.



3.1.2. IG (mg/ml)



kê 05



6

               
13,45±0,41 mg/ml.









9,95±1,52
a
10±2,05
a
11,1±1,48
a
11,78±1.93
a
10,93±2.63
ab

9,09±1,67
a
12,75±1,93
b
11,55±1,21
ab
13,07±2.20
a
12,43±2.23
b

10,86±2,9
a
11,98±3,49
b

10±2,18
c

12,26±2.22
a
10,63±2.70
a

10,86±2,9
a
13,45±1,74
b
12,35±2.02
bc
12,87±3.19
a
11,93±2.28
ab


 
mg/kg35±2,02 mg/ml
   ±2,18 mg/ml   
 
c, n
13,07±2,20 mg/ml. Tuy nhi

43±2,23
 10,63±2,70 mg/ml, 
  



i


3.1.3.  (U/ml)
H









82,05±3,18
a
90,27±4,68
a
92,51±2,12
a
92,54±0,54
a
93,92±1,02
a

88,82±1,45
a
126,45±7,75

b
117,32±9,94
b
147,83±7,34
b
213,09±1,32
c

84,26±2,77
a
126,45±1,63
b
135,44±7,27
b
205,30±1,11
c
193,01±10,74
b

83,33±4,00
a
128,97±1,29
b
123,83±5,62
b
234,83±7,86
d
200,03±2,74
bc




n    
              

7
             







levamisol




150 mg/kg                
213,09±1,32             


         
Hang et. al (2013
(pangasianodon hypophthalmus() mg LPS/kg

            
c


3.1.4.  (complement)
 (Unit)







4,53±1,04
a
5,46±1,40
a
6,86±2,73
a
6,16±0,84
a
6,68±1,35
a

5,21±1,30
a
10,61±1,83
b
10,43±1,05
b
11,98±1,45
b
12,67±1,43
c


5,82±1,24
a
10,34±2,52
b
11,37±1,47
b
11,10±1,47
b
11,01±1,45
b

5,43±1,54
a
12,25±1,82
b
12,22±1,61
b
11,88±2,11
b
11,55±1,19
bc


N

 không 

   ±    u 28       
levamisole không      


8

 1,61 Unit.

               

            






3.2.  E. ictaluri 
  
 không
,
. San gan
-                ctv
). 
sau khi gâym.5.
 (%)


 (%)
(%)

23,33±3,3
a

16,67±3,3
a

40,00±0,0
ab
63,33±3,3
b

60,00±10
c
50,00±5,7
b

43,00±3,3
bc
70,00±10
b

,05
 , cng levamisole
ng 3.5





u tiên. T

05. 
m 


9
.
 
 (2012) E. ictaluri (0,25x10
6
CFU/mL),
 



  k

 (2012
n E. ictaluri
5
CFU/mL




,


trê  E. ictaluri
NBT (mg/ml)
 (mg/ml)





0,08±0,04
a
0,07±0,03
a

0,09±0,04
a
0,53±0,16
b

0,12±0,02
a
0,20±0,07
a

0,10±0,05
a
0,51±0,18
b



,
 (p>0,05). H
04 mg/ml. K







    05.             E.
ictaluri (mg/ml)7

10






13,47±0,54
a
13,67±1,04
a

14,94±1,26
a
15,8±1,17
b

16,28±2,78
ab
13,53±1,13
a

18,70±3,71
b
14,47±1,15

ab


                


    71 mg/ml        
 28

    n    

17 mg/ml. 
 E. ictaluri
 3.8
8 

28


94,85±0,51
a
96,86±0,93
a

168,09±12,87
b
312,45±11,84
b

185,36±6,71

b
235,92±3,11
c


186,24±2,55
b
288,23±2,77
d



so mg/kg

                 


 (p<0,05)


 
 E. ictaluri     


11
ng 3.9 





10,26±2,80
a
12,10±0,77
a

12,25±0,84
ab
14,94±1,19
b

12,64±0,60
b
12,87±0,78
a

14,98±092
c
14,36±0,80
b



mg/kg mg/kg
 mg/kg




                




4. KT LU XUT
4.1. 


 150 mg/kg liên


E. ictaluri.
4.2. 




Bui Thi Bich Hang, Sylvain Milla, Virginie Gillardin, Nguyen Thanh Phuong,
Patrick Kestemont (2013) In vivo effects ofEscherichia colilipopolysaccharide on
regulation of immune response and protein expression in striped catfish
(Pangasianodon hypophthalmus). Fish & Shellfish Immunology 34: 339 - 347

(Pangasianodon hypophthalmus)Edwardsiella ictaluri


12

      Pangasianodon hypophthalmus 
-154.
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
Tae Sung (2012).(Pangasianodon hypophthalmus)

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
               
(Pangasianodon hypophthalmus). -142.

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