Molecular analysis of maternal diabetes induced changes in the developing neural tube
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A
A
Fig. 1
B
in
e
A
III
LV
E
B
dc
dc
e
vtw
vtw
E
C
vt
dt
D
Fig. 2
Thickness of Ventral Telencephalon
Wall (µm)
300
250
*
200
150
100
50
0
Fig. 3
Control Diabetic
vt
dt
vt
vt
A
B
Fig. 4
% of BrdU-cells in Ventral
Telencephalon
180
*
160
140
120
100
80
60
40
20
0
Fig. 5
Control
Diabetic
dc
e
dc
vt
A
Fig. 6
vt
B
e
A
Fold of induction
3.5
3
**
2.5
2
1.5
1
0.5
0
Control Diabetic
B
333bp
C
Fig. 7
dc
dc
III
vt
A
vt
B
LV
A
Fig. 8
dc
e
dc
III
vt
vt
LV
A
Fig. 9
dt
B
III
e
e
e
dc
vt
LV
A
Fig 10
B
A
Fold of induction
2
1.5
**
1
0.5
0
Control Diabetic
B
325bp
C
Fig. 11
e
dc
e
dc
vt
vt
dmt
dt
A
Fig. 12
B
*
A
Fold of induction
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Control Diabetic
B
238bp
C
Fig. 13
A
Fig. 14
B
C
B
III
III
A
B
ctx
LV
LV
LV
LV
cn
C
D
III
III
III
E
F
III
III
sn
hi
dn
G
Fig. 15
H
chp
bt
2
number of microglia (/mm )
45
40
35
30
25
20
15
10
5
0
Fig. 16
**
Normal
Diabetic
CP-treated
CP-treated diabetic
TGF-ß l
TGF-ß1
5A
tm
htms
III
III
A
B
htm
III
TGF-ß l
sn
III
TGF-ß l
hi
dn
chp
bt
C
D
TGF-ß l
TGF-ß l
III
III
E
F
TNF-α
III
G
Fig. 17
TNF-α
G
III
H
Lectin
TGF-ß1
A
B
C
Lectin
TNF-α
III
D
Fig. 18
TGF-ß1+Lectin
TNF-α+Lectin
III
III
E
F
tm
III
htms
III
htm
A
Fig. 19
B
50
Mean Cell numbers/mm
2
*
40
30
*
CP-treated diabetic
*
20
10
0
Fig. 20
TNF- α
TGF-β1
CP-treated
TGF-β1 mRNA
A
215bp
TNFα
B
241bp
IL-1ß
C
TGFß
173bp
D
370bp
iNOS
Fig. 21
folds of induction
8
7.5
7
6.5
6
5.5
5
4.5
4
3.5
3
2.5
2
1.5
1
0.5
0
Diabetic
CP-treated
CP-treated diabetic
TNF- α
Fig. 22
Normal
**
**
IL-1β
TGF-β1
iNOS
