Sử dụng kỹ thuật PCR RFLP trong nghiên cứu mối tương quan của đoạn Gene GH đến sinh trưởng của lợn rừng lai. (Khóa luận tốt nghiệp)

viii

-----------

-----------

:
GENE GH

: Chính quy
Ngành
Khoa

: CNSH & CNTP
: 2011-2015


viii

-----------

-----------

:
GENE GH

: Chính quy
Ngành
: 43
Khoa

CNSH

: CNSH & CNTP
: 2011

2015

:
2. PGS.TS Ngô Xuân Bình

Thái Nguyên


viii

bè và gia

.

Em xin chân thành c

p và hoàn

Thái
Sinh viên


viii

Trang

DdeI............................................... 12
........................... 24
........................................ 25
gene GH............................ 31
gene GH
DdeI ................................................................................................................. 32
260 nm/OD280 nm

................................ 37

GH

................. 41
GH

. 43

(kg) .................................................................................................................. 44

(g/con/ngày) .................................................................................................... 46


viii

Trang
Hình

.................................................. 9
GH

........................................ 13
..................................... 14

GH b

DdeI................................................................................ 15
Dde

gene GH........................................................................................................... 16
Hình 2.6

n gene GH

Dde
gene GH......................................................... 17
GH

enzyme DdeI....... 27

......................................... 30
GH ...... 31

H

35
................................. 36
Hình 4.3: S

GH ........................................ 39
GH

DdeI ..................... 40

............................................................................................................... 42
GH................... 44


viii

Bp

: Base paire

DNA

: Deoxyribonucleic acid

dNTP

: Deoxyribonucleoside triphosphate

EtBt

: Ethidium bromid

TBE

: Tris boric acid

TE

: Tris

RNA

: Ribonucleic acid

PCR

: Polymerase chain Reaction

RFLP

: Restriction Fragment Length Polymorphism DNA

OD

: Optical density

v/p

: Vòng/phút

EDTA

EDTA

Cs
CIAA

: 24 Chloroform: 1 Isoamylacohol


viii

Trang
:

........................................................................................... 2
....................................................................................................... 2
...................................................................... 2
........................................................................................... 2
..................................................................................2
.........................................................................3

:

................................................................. 4
.................................................................. 4
............................................................... 5
............................................................................................... 5
................................................5
........................................................... 5
.............................................................. 6
............................... 6
....................6
...................................................................6

2.3.4

....................................................7
...............................................................8
...............................9
-RFLP ...........................................................................10
GH (Growth Hormone).................................. 12
phân

GH

DdeI 14

DdeI trên gene GH ..................................... 15
................................................. 18


viii
2.7

.....................................................18

2.7.2. Tình hình
:

.......................................................20
.. 23
............................................................... 23
.........................................................................23
............................................................................23
................................................................... 23
...........................................................................23

.............................................................................23
............................................................... 23

................................................................................................23
...............................................................................................24
.......................................................................................25
.................................................................................... 26
...................................... 26
.............................................................................27
...........................................................................27
....................................... 27
3.5.2.2.

......................................... 27
....................................................................28
.......................................................................... 28
.................. 28

3.5

.................................................................... 30
-RFLP ......................................................... 32
................................................ 32
.33

:

.............................. 35

viii
4.1.

......... 35
gene GH................................................... 36
.....................................................................36
................................... 36
260nm/OD280nm

................. 37
......................38
e GH

DdeI ........................39
GH

....................................................................................... 44
:

............................................................. 49
......................................................................................................... 49
.......................................................................................................... 49
............................................................................... 51
......................................................................................................... 51
........................................................................................................ 52


2

(

, 2012) [1]

.
,
:

.

(

, 2010) [12].

. Ngày nay,


2

e
(
al, 2010) [6]. Gene GH (Growth Hormone

Hormone sinh

(GH
(Mariusz Pierz
2004) [22]

(C. Knorr, G.Moser et al, 1997) [15]

(Nielsen, Larsen et al, 1995) [24].
vào

-

gene GH
.

-N

GH

.
phân

1.3. Ý

-

gene GH
e GH

gene GH


3

-

gene GH

.

-

nuôi,


4

(

, 2010) [12].

-

- 40kg.

tính hoang dã.
y,

(
, 2010) [12].


5

1770,
scrofa.

: Toàn

- 380 kg,
-

-55 % (

2008) [7].
.


6

-

-

:
-

:

,

,

.
.
,

(R):

(

%

, 2008) [4].

,


7

PCR (PCRarker)
(

, 2008) [4].

2.3.4

-

-


8

-

-196oC

cá

(phenol:

rotein

(Na+, K+, hay NH4+
t


9

Chain Reaction)
1985.

(

) [8].


10

-95o

-70o
-72o

-

(

-RFLP

, 2006) [8].


11

(Ngô Xuân Bình, 2004) [2]
PCR.

sau
.
e
e hoàn toà
(

, 2012) [9].

(
2010) [6].
-

(Wang, Zhang et al, 2009) [27].


12

GH
Dde
gene GH

.

Dde

DdeI

GH/DdeI

Desulfovibrio
desulfuricans
GH (Growth Hormone)
GH
(

Tadeusz Blicharski et al, 2004) [22]. Growth Hormone (GH)
(Kato,
Shimokawa et al, 1990, Yerle, Lahbib-Mansais et al, 1993) [20,28]. Gene GH
(Yerle, Lahbib-Mansais et
al, 1993, Chowdhary, Thomsen et al, 1994)
(Vize PD and Wells JR, 1987) [26].
GH có
(Cogan and
Phillips, 1998) [16]

stress (C. Knorr, G.Moser et al, 1997)
-Yoan) có
GH (Cheng, Lee et al, 2000) [17]. Nielsen và Larsen
GH

13

GH
prote

(Nielsen VH and Larsen NJ, 1991) [23].
GH
accession: M17704.1).
GH
Phân
GH (605 bp)

GH
GH
* Gene GH
-

nucleotide 652.

GH
GH

GH


14

GH
GH

DdeI
Dde

Alen D1: 335 bp; 148 bp; 122 bp
Alen D2: 457 bp; 148 bp
GH.

-

GH

.3
a) Alen D1

148 bp
335 bp
122 bp

b) Alen D2
148 bp
457 bp


15

148 bp
335 bp
122 bp

148 bp
335 bp
122 bp
457 bp

148 bp
457 bp

GH
2.6

DdeI

DdeI trên
gene GH

DdeI trên
Dde

gene GH

.
.

gene GH


16

ne

GH (605 bp)

DdeI trên
GH
GH

Hình 2.5
DdeI. Theo
GH

(2005) [19]

GH

enzyme Dde

122 bp (Franco, Antunes et al, 2005) [19].
GH

Dde

GH
GH

DdeI, do

GH

DdeI và