LCMS MS based metabolome analysis of babylonia

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LC–MS/MS-Based Metabolome Analysis of
Biochemical Pathways Altered by Food
Limitation in Larvae of Ivory Shell,
Babylonia areolata
AuthorsAuthors and affiliations
17-22 phút
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Jingqiang Fu
Minghui Shen
Yawei Shen
Wengang Lü
Miaoqin Huang
Xuan Luo
Jinjin Yu
Caihuan Ke

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Weiwei You

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Jingqiang Fu
o 1
o 2
Minghui Shen
o 1
o 2
o 3
Yawei Shen
o 1
o 2
Wengang Lü
o 4
Miaoqin Huang
o 1
o 2
Xuan Luo
o 1
o 2
o 5
Jinjin Yu
o 1

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o

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2
Caihuan Ke
o 1
o 2
o 5
Weiwei You
o 1
o 2
o 5
Email authorView author's OrcID profile

1. 1.State
Key
Laboratory
of
Marine
Environmental
ScienceXiamen
UniversityXiamenPeople’s Republic of China

2. 2.College of Ocean and Earth SciencesXiamen UniversityXiamenPeople’s Republic of
China
3. 3.Tropical Marine Products Fine Breed CenterHainan Academy of Ocean and Fisheries
SciencesHainanPeople’s Republic of China
4. 4.College of FisheriesGuangdong Ocean UniversityZhanjiangPeople’s Republic of China
5. 5.Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine
Biological ResourcesXiamenPeople’s Republic of China
Original Article
First Online: 20 April 2018

Abstract
Ivory shell, Babylonia areolata, is one of the commercially important mariculture species in
China and South East Asia. Survival varies in the artificial hatching and larval rearing of B.
areolata. Food deprivation may be involved in rearing mortality, and so, a better understanding
of how larvae respond and adjust to starvation is needed. In this study, the metabolite profiles of
newly hatched larvae with yolk (I), larvae with yolk exhaustion (II), larvae suffering 24 h
starvation after yolk exhaustion (III), and larvae fed with exogenous nutrients after yolk
exhaustion (IV) were analyzed by LC–MS/MS. Principal component and cluster analyses
revealed differential abundance of metabolite profiles across groups. When compared to
metabolite levels of the I group, significantly up-regulated metabolites included polyunsaturated
fatty acids, phospholipids, nucleotide, amino acids, and their derivatives were found in the II
group, indicating that organisms relied predominantly on glycerophospolipid metabolism and
protein-based catabolism for energy production during this stage. During starvation after yolk
exhaustion, the levels of all energy related metabolites were significantly reduced, but an
increase in products of purine and pyrimidine metabolism indicated an insufficient energy supply
and an increase in cellular disintegration. Larvae fed exogenous nutrients can have significantly
improved metabolism compared to starved larvae. These findings suggest that metabolomics,
using LC–MS/MS, can be used to assess the physiological status and food-affected metabolic
changes affecting B. areolata larvae.

Keywords
Babylonia areolata LC–MS/MS Metabolomics Newly hatched larvae Food limitation
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Notes
Acknowledgements
The authors thank the Hainan Academy of Ocean and Fisheries Sciences for its assistance in
operations over the larval periods. This research was supported by the Earmarked Fund for
Modern Agro-industry Technology Research System (No. CARS-48) and the Hainan Provincial
Ocean Basic Budget Project in 2016.

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