Study on genotypic differences in photosynthetic nitrogen use efficiency, nitrogen use efficiency, yield and yield related traits in different rice genotypes under different nitrogen levels

Int.J.Curr.Microbiol.App.Sci (2019) 8(4): 2484-2493

International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 8 Number 04 (2019)
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Original Research Article

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Study on Genotypic Differences in Photosynthetic Nitrogen Use Efficiency,
Nitrogen Use Efficiency, Yield and Yield Related Traits in Different Rice
Genotypes under Different Nitrogen Levels
V. Aparna1*, S. Narender Reddy1 and D. Subrahmanyam2
1

Department of Crop Physiology, Professor Jayashankar Telangana State Agricultural
University, Hyderabad, Telangana, 500030, India
2
Department of Plant Physiology, IIRR, Rajendranagar, Telangana, 500030, India
*Corresponding author

ABSTRACT

Keywords
Nutrient,
Genotypes, Rice,
Nitrogen use
efficiency,
Recommended dose
of nitrogen

Article Info
Accepted:
17 March 2019
Available Online:
10 April 2019

Nitrogen is the major nutrient element required for the plant growth and development. In
rice, development of varieties with improved nitrogen use efficiency will reduce the
nitrogen fertilizer application. This study was investigating the genotypic differences in
photosynthetic nitrogen use efficiency, nitrogen use efficiency, yield and yield related
traits of 12 different rice genotypes under two different nitrogen levels viz., T1 and T2.
Different physiological and agronomical characters were measured in selected 12
genotypes, growing in field. The photosynthetic rate was recorded higher (23.3 μmol CO 2
m-2 s-1) with rice genotype Sampada. Efficiency parameters including Photosynthetic
Nitrogen Use Efficiency and Harvest Index were studied in order to determine NUE.
Chlorophyll content was recorded maximum in vegetative stage under 100% RDN and in
reproductive stage under T1 and T2 in NUE genotype of Vardhan and minimum in low
NUE genotype Sampada x Jaya/3 under T1 and T2 treatments. Total Dry Matter, grain
yield and leaf nitrogen content were higher in high NUE genotype Vardhan. High
chlorophyll content in case of Vardhan under low nitrogen content support their higher
grain yield and total dry matter content. The Nitrogen Use Efficiency (7.11) and
photosynthetic nitrogen use efficiency (12.50) were lower when treated with 100% of
recommended Nitrogen. The maximum average grain yield of 824 g m-2 was registered in
Vardhan genotype.

Introduction
Rice (Oryza sativa L.) is one of the most
important food crop grown and is consumed
by one-third of the world’s population. In
order to support the increasing population the

yields has to be increased. Nitrogen is an
important primary constituent of the

nucleotides,
amino
acids,
proteins,
chlorophyll and several plant hormones. It is
one of the most important nutrients in
improving crop yields. Nitrogen is
responsible for improving panicle number and
grain weight and responsible for reducing
spikelet sterility (Fageria, 2009). Though,
application of nitrogen fertilizers increases
crop yields, increased use of N fertilizers

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Int.J.Curr.Microbiol.App.Sci (2019) 8(4): 2484-2493

effects global N cycle, depletion of ozone
layer and also causes nitrate leaching
problems in soil (Hakeem et al., 2012). Out of
the total amount of applied nitrogen, only 30–
40% of the applied N reaches the plant and
the remaining is lost to the environment
(Raun and Johnson, 1999). Hence, using Nefficient genotypes is an important strategy in
reducing cost of production as well as
environmental pollution.

Nitrogen use efficiency has been defined as
grain yield per unit of N available in the soil
(Good et al., 2004). At higher N rates, the
decrease in NUE indicates that rice plants
could not absorb or utilize N at higher rates or
that N loss exceeded the rate of plant uptake.
Nitrogen use efficient genotypes can be
defined as plants which can absorb and
accumulate higher N content and grow well
and yield better under low N conditions (Mi
et al., 2007). NUE is relatively low in rice as
major part of N applied to rice is released as
gaseous N, effecting environment and
reducing econo0.6
0.6
0.7

0.7
0.7
0.6

0.6
0.6
0.7

8.9
7.9
8.0

9.8

8.9
9.1

9.4
8.4
8.5

296
311
303

296
288
291

296
299
297

18.4
20.1
16.4
22.0
17.9
20.4
19.5
19.6

24.1
19.1

24.2
24.6
23.6
23.1
20.9
23.3
0.4
1.04
1.5

21.2
19.6
20.3
23.3
20.8
21.7
20.3
21.4

0.7
0.7
0.5
0.5
0.5
0.6
0.5
0.6

0.7
0.7

0.6
0.8
0.7
0.6
0.6
0.7
0.1
0.1
0.2

0.7
0.7
0.6
0.7
0.6
0.6
0.5
0.7

8.2
8.8
7.3
8.1
8.2
8.2
7.7
8.2

9.4
10.1

8.8
9.6
9.9
9.0
9.2
9.5
0.2
0.6
0.8

8.8
9.5
8.1
8.9
9.1
8.6
8.4
8.8

319
315
303
288
302
296
292
302

288
312

288
304
301
295
297
298

304
313
296
296
301
295
295
300

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Int.J.Curr.Microbiol.App.Sci (2019) 8(4): 2484-2493

Table.4 The influence of nitrogen on partial factor productivity of nitrogen
in different rice genotypes

Genotypes
Varadhan x BPT 5204/6

Varadhan x BPT 5204/10
Sampada x Jaya/2
Sampada x Jaya/3
Varadhan x MTU 1010/2
Rasi x Jaya/2
Varadhan
BPT-5204
Sampada
Jaya
MTU-1010
Rasi
Mean
Treatment (T)
Genotypes (G)
TxG

Nitrogen Use Efficiency
T1
T2
15.88
6.30
10.40
6.70
13.26
8.38
9.52
5.32
14.44
7.59
15.78

8.15
15.94
8.51
11.84
6.15
12.88
7.91
13.86
8.70
16.38
4.06
15.22
7.49
13.78
7.11
0.14
0.10
0.17

Mean
11.09
8.55
10.82
7.42
11.02
11.97
12.23
9.00
10.40
11.28

10.22
11.36
10.44

Table.5 The influence of nitrogen on root, stem and leaf nitrogen in different rice genotypes
Genotypes
Varadhan x BPT
5204/6
Varadhan x BPT
5204/10
Sampada x Jaya/2
Sampada x Jaya/3
Varadhan x MTU
1010/2
Rasi x Jaya/2
Varadhan
BPT-5204
Sampada
Jaya
MTU-1010
Rasi
Mean
Treatments (T)
Genotypes (G)
TxG

Root nitrogen (%)
T1
T2
Mean

0.72
0.80
0.76

T1
0.43

Stem N(%)
T2
Mean
0.50
0.46

T1
1.28

Leaf N(%)
T2
Mean
2.12
1.70

0.70

0.71

0.71

0.64

0.65

0.65

1.50

1.84

1.67

0.71
0.57
0.72

0.79
0.82
0.92

0.75
0.70
0.82

0.58
0.66
0.50

0.79
0.73
0.51

0.69
0.70
0.51

1.85
1.75
1.66

1.98
2.00
1.98

1.91
1.88
1.82

0.65
0.86
0.72
0.68
0.73
0.65
0.99
0.73

0.88
0.75
0.79
0.81
0.78

0.80
0.95
0.82
0.06
0.05
0.06

0.77
0.81
0.76
0.75
0.76
0.73
0.97
0.77

0.50
0.40
0.57
0.62
0.42
0.36
0.64
0.50

0.52
0.48
0.77
0.68
0.81

0.60
1.06
0.70
NS
0.19
NS

0.51
0.44
0.67
0.65
0.62
0.48
0.85
0.60

1.94
1.68
1.91
1.51
1.57
1.47
1.45
1.60

2.19
2.35
1.93
1.66
1.91

1.65
1.53
1.90
NS
NS
NS

2.07
2.02
1.92
1.59
1.74
1.56
1.49
1.80

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Int.J.Curr.Microbiol.App.Sci (2019) 8(4): 2484-2493

Table.6 The influence of nitrogen on photosynthetic nitrogen use efficiency and Harvest Index
in different rice genotypes
Genotypes (G)

Photosynthetic rate/ Harvest index (%)
Leaf N
T1

Varadhan x BPT

5204/6
Varadhan x BPT
5204/10
Sampada x Jaya/2
Sampada x Jaya/3
Varadhan x MTU
1010/2
Rasi x Jaya/2
Varadhan
BPT-5204
Sampada
Jaya
MTU-1010
Rasi
Mean
Treatments (T)
Genotypes (G)
T xG

T2

Mean T1

T2

Mean

23.15 11.84

17.50

51

46

48

12.64 12.69

12.67

45

48

47

11.80 12.29
10.83 12.17
13.26 12.31

12.05
11.50
12.79

49
46
49

48

43
49

48
44
49

9.48
12.13
8.55
14.77
11.66
13.86
13.50
12.97

10.25 49
10.12 48
10.73 47
14.80 48
12.05 45
13.93 46
14.44 45
12.73 47.3

11.02
8.10
12.90
14.83
12.44

14.00
15.37
12.50
0.80
0.17
0.29

In conclusion, present study found that there
are wide variations in physiological
parameters, grain yield, N content in different
plant parts as well as nitrogen use efficiency,
grain yield efficiency and harvest indices
among genotypes under two nitrogen regimes.
With the exception to Vardhan x BPT 5204/6,
MTU-1010 and Rasi all the entries included
in this field experiment showed reduction in
grain yield under 50% RDN. Vardhan and
Rasi x Jaya/2 recorded highest average grain
yield with high nitrogen use efficiency.
Hence, they were identified as nitrogen
efficient genotypes because they consistently
produced high grain yield.

50
53
43
48
53
29
50

46.5
NS
NS
NS

50
50
45
48
49
38
47
46.9

Acknowledgement
I acknowledge the help received from Indian
Institute
of
Rice
Research
(IIRR),
Rajendranagar, Hyderabad during the
research work.
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How to cite this article:
Aparna, V., S. Narender Reddy and Subrahmanyam, D. 2019. Study on Genotypic Differences
in Photosynthetic Nitrogen Use Efficiency, Nitrogen Use Efficiency, Yield and Yield Related
Traits
in
Different
Rice
Genotypes
under
Different
Nitrogen
Levels.
Int.J.Curr.Microbiol.App.Sci. 8(04): 2484-2493. doi: />
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