tạo hoa hồng xanh bằng kĩ thuật gene

BLUE ROSE TRANSGENIC
PLANT


Agenda Style
01

Introduction

02

method of gene transfer

03

applying


Our Team

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Ph.D Tran Thi Dung

Van Pham Nhu Ngoc

lecture

student

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Introduction


 

In 2004, researchers used genetic modification to
create roses that contain the blue pigment
delphinidin..

01

The blue rose is a flower of the genus Rosa
(family Rosaceae) with the prefix from blue
to purple

02


What is

RNAi is involved in controlling gene activation in the post-

RNAi?

transcription phase

•

RNAi is the process of silencing a gene, inactivating a target gene with a sequence
similar to that of the gene inactivating agent (short strands of RNA about 21-27
nucleotides).


Basic features of the RNAi technique

 Specificity, high efficiency, very sensitive.
 Inactivates genes of individuals at different stages, inherited from generation to generation,
tissue to tissue.

 The dsRNA design is easy if the genome sequence is known.



Method of gene transfer


remove the gene encoding the enzyme
dihydroflavonol reductase (DFR) in roses.

Application of RNAi technology for
S
ES
KN
EA

ST
RE

N
G
TH
S

W

gene replacement in plants

created the 'silencer' gene to exploit a
TH
R
E
AT
S

complete the delphinidin-synthesis reaction.

TU
R
O
PP
O

An iris gene for an enzyme, DFR, required to

ES
TI
I
N

SWOT

cellular phenomenon called RNA
interference (RNAi)


Creating blue roses using RNAi technique


CHU TRÌNH HÌNH THÀNH SẮC TỐ TRÊN CÂY TRỒNG

Anthocyanin dihydrokaempferol enzyme controls
all three cycle of plant pigment formation
including: cyanidin, pelargonidin and delphinidin.




RNAi technique to inhibit the activity of the DFR gene in red roses  leads to inhibition of
the


delphinidin-transgene
complete

cyanidin
the

cycle

with

delphinidin

a

complete
synthesis

DFR

gene
cycle.

=> using RNAi technique to inhibit the DFR gene  clone a new delphinidin gene from
pansy and DFR gene from iris flower  DFR gene inhibition of roses without affecting the
DFR gene of iris flowers


Cell pH is one of the main reasons flowers have

Petal ph cell concentrations

the same anthocyanin cycle but have different
colors.

1

Influence of

4

are often genetic

Ordinary rose petals have a pH

While the cell pH is alkaline, the pigment
of anthocyanin usually becomes greener.

5

2

concentration of about 4.5.

Scientists have just come up with RNAi gene-suppressing

pH

Soil pH has little or no effect on the
ph of the petal cells.

3

techniques to identify genes that affect petal acidity or

6

adjust petal color in other directions.

3

Applying


The application of RNAi technology not only in creating blue
roses but also opens up a new era for the application of
Unique flower means making huge

genetic engineering in agriculture.
01

04

profits.

In addition to creating a blue rose, the scientist also
creates a variety of flowers with different colors,

In the global commission business with sales of
1.2 billion USD / year

02

05

The market for green roses is mainly in: America,
Australia and Japan

03

fragrances and perils

The demand for roses, cuttings accounts for
06

50% of the flower market.


Thank You
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