Khảo sát tác dụng hạ đường huyết của một số loại thảo dược trên mô hình chuột in vivo

TAP CHI KHOA HOC TRIJONG DAI HOC Ma TP.HCM - SO 4 (37) 2014

11

KHAO SAT TAC DUNG HA DUONG HUYET CUA
MOT SO LOAI THAO DON MEN MO HiNH CHUOT IN VIVO
Ngiy nhin bii: 19/04/2014
Ngay nhin lai: 19/06/2014
Ngay duylet ding: 07/07/2014

Ho Thi Huyen Trang, Phan: Thi Ng9c Bich,
Pham Xuan Xinh, Trwong Thi B9ch Van,'
Vu Tien Luyen2
Lao Dem Thuan3

TOM TAT
Dai thcio &tang la chirng benh thuitng gap tren the giol veri bleu hien la su tang cao ye
&rang huyet do bat thuang trong tac clang caa insullin. Hien nay, viec sir clung cac loaf thcio
chew trong cac bai thuoc dan gian clang dugs quan tam do cluing mang nhieu lai ich. Trong
nghien caw nay, chang tOi tien hanh khcio sat Hui nang ha dyeing huyet caa cao chiet trai dau
bap, hat methi, re hoang lien va tra Kombucha tren mo hinh chuat dai thao clueing. Ket qua cho
thay cao chief- trai dau bap (40 g/kg), hat methi (30 g/kg) va re cay hoang lien (150 mg/kg) c6
cho tac clung manh
tac ,dting clueing huyet. Trong do, cao chiet dau bap va cciy hoang
nhcit gitip ha &tang huyet chuat tic 500 mg/dl xuang du& 200 mg/dl. Ngoai ra, cac chi tieu sinh
lv chuat thi nghiem deu a mar on dinh. Tir do, cluing toi ket luan cao chiet con trai dau bap, hat
methi va re cciy hoang lien co tac dung hg clueing huyet a nhiing lieu duos khcio sat va an toan
vai chuOt thi nghiem.
Tir khoa: Benh dai thao &row, mo hinh chuOt dai thao throng, dau bip, hat methi, Hoeing
Lien.
ABSTRACT

Diabetes mellitus is a common worldwide disease with hyperglycaemia due to abnormal
activities of insullin. The use of traditional medicine in treatment of diabetes has gained more
attention recently because of its convenience. In this study, the hypoglycaemic effects of the
ethanol extracts from water okra fruit, methi seeds, Coptis teeta Wall (hoang lien) roots and
Kombucha tea are carried out on diabetic mouse model induced by alloxan. Results show that
water okra fruits (at 40 g/kg), methi seeds (at 30 g/kg) and hoang lien roots (at 150 mg/kg)
posess hypoglycaemia effect. Especially, water okra fruits and hoang lien roots reduce blood
glucose from the high level (> 500 mg/dl) to normal level (< 200 mg/dl) without affecting mouse
normal physiology. Thus, we conclude that the extracts of water okra fruits, methi seeds and
hoang lien roots have the anti-hyperglycaemic effects in diabetes.
Keywords: Diabetes, diabetic mouse models, water okra, methi seeds, Coptis teeta Wall.

TruCrag Dgi hpc Ma TP.HCM.
Trufmg Dgi hoc Khoa hpc Tip 1Vhien TP.HCM, Dgi hpc Quoc Gia TP.HCM.
3 ThS, Tneang Doi hoc Ma TP.HCM Email:
I

2


CONG NGHE

12
1. flat van de

Dai thao, dueing, (Diabetes mellitus) la
chirng benh ve chuyen hoa clueing huyet181.
Bleu hien lam sang, ciia benh la six tang cao
ming de clueing huyet do str thieu but insullin
(type I) hoac do sai hong trong hoat (king ciia

insullin (type II) gay anh hoang den cac qua
trinh chuyen hoa carbonhydrate, chat beo va
protein1121. Theo thong ke vao nam 2013, Viet
Nam c6 khoang 3,3 trieu ngtroi benh. chiem ti
le 5,37% dan se, la met trong nam mac c6 so
ca mac benh cao nhat a khu vtrc tay Thai Binh
Duane'. Dieu clang ghi nhan rang ty le mac
benh dai thao duang tren the gioi ngay cang
gia tang clang ke, theo dtr doan vao nam 2025.
so ca mac benh tang den khoang 52.7 trieu
ngueri (5.4% dan so the gieri)181. Ve dieu tri,
hien nay, ben canh cac loai thuoc thuang mai
tren thi trtremg, benh nhan c6,the dieu tri bang
cac phtrang phap y hoc co truyen vi cac
phtrang phap nay it ton kern, it gay tac dung
phu. der (lilac sir dung lau dai trong dan gian va
der dugc xay dung hoan chinh bai ban. ket hop
gifia viec sir dung thao dugc va cac phtrang
phap chira tri be trai l 11. Chinh vi very, cac loai
thao dugc tir nhien co tac dung dieu tri hay hO
trg dieu tri benh dai thao clueing dang dugc tap
trung nghien dru va phat trien nham thay the
cac thuoc tong hop hoa hoc dang dugc sir dung
hien nay1111.
Trong dan gian, met s6 loai did° dugc
dugc cho la c6 ding dung hi; trg dieu tri dai
thao duang chang han nhtr dau bap
(Abelmoschus esculentus), re cay hoang lien
(C'optis teeta Wall), hat methi (Trigonella
L.),

tra
foenum-graecum
0103104105i.
Tuy nhien, hien nay
Kombucha....
nhang nghien cfru de cap den kha nang ha
duang huyet cua cac loai thao dugc tren O. Viet
Nam van con han che. Xuat phat tir thuc to
tren va dua tren nhanp ket qua ciia Lao Dire
Thuan va cs (2013)131, Chung ten tien hanh
nghien cuu khdo sat kha nang 110 trg ha clueing
huyet cua cac chiet xuat cao con fir trai dau
bap. hat methi, re cay hoang lien va tra
Kombucha tren mo hinh chuet bach (Mos
musculus var. Albino) bi benh dai thao duang
bai tac nhan hoa chat Alloxan (A7413, Sigma)
itham tao tier) de cho viec nghien dru Oat

trien nhOng bai thuoc dan gian co tac dung 113
trg dieu tri ha duang huyet.
2. Phulyng phi!) nghien cuu
2.1. Sv luvc mot so Mao doge dwvc set
dung trong nghien au
Dau bap (Albelmoschus esculentus) la
loai cay than thao hang nien, thuerng dugc
trong a khap Viet Nam., Qua dau bap co tac
dung On dinh clueing huyet trong mau, giam xa
vita deng mach. ngan chan skr di can cna ung
thu dai true trang va met se tac dung khac131.
Cay methi (Trigonella Ibenum:graecurn

L.) la loai cay than thao hang nien, trong nhieu
cac nuo.c Nam A va viing Dia Trung Hai.
Hat methi dugc sir dung nhu on loai thuc
pham chirc nang co ,tac dung On dinh clueing
huyet, giam cac roi loan tieu hoa, giam
cholesterol va met so tac dung khacl".
Cay hoang lien (C'optis teeta Wall) la
loaf
thaodtrac mac tren day Floang Lien
mot
San. Phan than va re cua cay c6 tac dung
khang khuan, khang virus va On dinh duang
huyet ding met so tac dung. khacI31.
Tra Kombucha la loai tra len men tir he
vi khuan Acetobacter va met so loai nam men
thuec chi Zygosacchromyces. Brettanomyces.
Tra Kombucha co tac dung tang cueing sirc de
khang, phong ngira va dieu tri tieu duang, cai
thien chirc nang gan, roi loan tieu h6a va cac
tac dung dirge 1/ khacl".
2.2. Thu nhiin cao chiit ethanol trai
dim bap, kit methi va re cay hang lienI31
Dan bap, hat methi va re cay hoang lien
dtrgc phai kha, sau do cac loai dugc lieu nay
dugc xay nho va ngain vai ethanol 850 .theo ty
le 1:1. Sau 5 ngay, dich chiet dugc co quay duoi
dung mai cho den khi tao thanh han hgp set.
2.3. Thu 'ikon dung dich tra Kombucha
Ntrac cat dun soi vai duang theo ty le
10:1 va b6 sung cac tra tai lac co san tren thi

truOng. Sau do, dich tra dugc b6 sung them
con men (0,3% w/v) va dich tra Kombucha c6
san (0,3% v/v). Tra dugc u a nhiet de phong
trong tir 6 den 15 ngay. Tra sau khi u co pH =
2,5-3,0.


13

TAP CHI KHOA HOC TRUaNG DAI HOC MO TP.HCM - S6 4 (37) 2014

2.4. D'Ong vitt thi nghiem
ChuOt bach (Mus musculus var. Albino)
c6 trong ltrang trung binh 22,5 ± 2,5 g dtrac
mua tir vien Pasteur Thanh ph6 H6 Chi Minh
va nu6i duang tai phong thi nghiem sinh ly
d6ng vat, Twang Dai hoc Ma TP.HCM vai
chu kSt sang tai 12/12. ChuOt ducc nuoi on
dinh 3 ngay truck khi thi nghiem.
2.5. T90 mo hinh chit& tiau
bang Alloxani31
ChuOt &pc chia thanh hai 16 thi nghiem.
Lo 1 tiem Alloxan (200, mg/kg) va 10 2 tiem
mak cat. Sau 2 gi6, tien hanh tiem 0,5 mL
dung dich glucose 5% o ca hai 16., Sau 72 gio.,
chu6t dtrac kiem ,tra dithng huyet. Chu6t co
gia tri duang huyet tren 200 mg/dl duac xem
nhu thanh cong va sir dung cho nhi-mg thi
nghiem khao sat kha nang 116 try ha &rang
huyet sau nay.

2.6. Kith° sat Mc dung h9 throng huyb
can cac loci thdo &rot.

Chu6t sir dung trong thi nghiem nay deu
tang thai benh dai thao twang voi chi so
throng huyet 16n han 200 mg/dl. Chu6t ducyc
chia thanh 6,10, m sai 16 7 con. Lo doi chirng am
cho chu6t Ong ntrac cat. L6 doi chi:mg duang
cho chu6t uong Gliclazide (Stada) co tac dung
ha duang huyet. cac 16 thi nghiem 1, 2, 3 va
4, cac chu6t ducyc tien hanh cho uong theo thin
to nhtr sau: cao chiet tau bap (40 g/kg), cao
chiet hat methi (30 g/kg), cao chiet re cay
hoang lien (150 mg/kg) va tra Kombucha (0.09
ml/con). moi ngay uong hai linI31141161191. Cac.
chi so ye throng huyet, trong luting. sinh ly
mau dtrac kiem tra trong khoang thai gian
trong 20 ngay thi nghiem.
2.7. Hwang phdp xer ly thong ki
S6 lieu va ket qua thu nhan dtrac phan
tich va thong ke bang cong cu ANOVA trong
phan mem Microsoft Excel 2007 va phan mem
Statgraphic 3.0. Ket qua duac trinh bay du.61
Bang MEAN ± SEMI21.

3. Ket qui va thio 1u4n
3.1. Ket quii too mo hinh chu0 lieu &ton being Alloxan
Bing 1. Su. thay dai ding do duirng huyet Marc va sau khi tiem Alloxan 72 giir
Chi so duirng huyet
&trot khi tiem Alloxan

(mg/di)

Chi so duirng huyet
sau khi tiem Alloxan
(mg/di)

Ty 1e thanh cong
(%)

La 1 (200 mg/kg)

134,0 ± 6,1*

368,3 ± 21,7**

70 %

La 2 (nircrc cat)

133,4 ± 7,4a

135,8 ± 6,5a

0%

Ghi chit: Khac biet

coy nghTa thong kip voi do tin ca'y 95 %.

ChuOt sau khi tiem Alloxan lieu 200

mg/dl c6 sir tang len rO ret ve chi so twang
huyet sau 72 gio thi nghiem
ti le thanh
ding la 70%, trong khi 16, chimg am khong
thay co sir tang len ye chi so dui:mg huyet. Ket
qua tren phii hop yen nghien ciru truck ctla Lao
Dirc Thuan va cs (2013) trong xay dung mo
hinh chuOt tieu duang bang Alloxan131. Ngoai

ra, phuang phap nay cling cho ket qua tuang
by yeti cac phirang phap gay dai thao duang
chu6t cac nh6m nghien ciru khac nhau tren
the
Nhu vay, c6 the khing dinh
ring chiing tai da xay dung thanh ding m6
hinh chu6t tieu dtrOng va co the sir dung cho
cac nghien ciru tiep theo.


14

GONG NGHE

3.2. Dui niing ha throng huyit ciia cao chiet trai dnu biip, hat methi, re cay holing lien
va dung dich Ira Kombucha
Bang 2. Su' thay &di tiling di) throng huyet (mg/di) trong thoti gian Maio sat
NO

N5

N1O

N15

N20

532.85 ± 2.56

528,86 ± 4,36

527,00 ± 6,33

524,00 ± 3.25

527,29 ± 4,20

Le dOi ching throng 531,29 ± 4,22

470,71 ± 7,53

362,57 ± 7,06

239,43 ± 6,49

173,57 ± 4.83

Lo dOi chting am

Le 1 (Dau bap)

528,57 ± 5,14 418,49 ± 14,16 328,80 ± 22,34 241,92 ± 16,37 186.75 ± 6,28

Le 2 (Hat methi)

526,43 ± 6,83

434,43 ± 7,94 334,29 ± 10,98 281,71 ± 15,61 256,57 ± 18,67

Lo 3 (Re lining lien) 519,57 ± 22,05 480,86 ± 25,57 486,29 ± 22,58 381,14 ± 13,38 172,00 ± 16,08

515,43 ± 2,74

Lo 4 (Kombucha)

511,57 ± 1,77

505,43 ± 7,61

497,14 ± 7,91

499,00 ± 9,04

Hinh 1. DO thi bieu (lien sty bien thien tiling di) dtrirng huyet cna chuOt thi nghien
600

AM

-a- De IN. row;
-to- PAU BAP

300

-110ANO LIEN
K(
0
NO

N5

NIO
Nt.:AV

DO thi a Hinhl cho thay, lo chirng am
(chuiit duct cho uong ntroc cat) khong co su
thay doi ve nOng (10 duang huyet Oren 500
mg/dl) ching to chuOt van a trang thai benhl
dai thao throng. Cac lo thirighiern 1, 2 va 3 co
tac dung ha throng huyet sau 20 ngay thi
nghiem.Trong do, cao chiet dAu bap va re
hoang lien có tac dOng tuang to
lo doi
chang ducmg ye kha rang ha duang huyet
chuOt (chirng throng: 173,57 mg/dl, dau bap:
186,75 mg/di, hoang lien: 172,00 mg/dl) va
dua chi so duong huyet . mire binh throng
(106-278 mg/d1)1 1. lo 3, cao chiet methi co
kha nang ha duemg huyet cua chuOt (256,57
mg/di) nhung chin ha duce &rang huy8t cluai
mire 200 mg/d1. Tuy nhien, ket qua dm tra

NIS N20
NGI-11101

Kombucha (164) kh6ng có tac dung ha &rang
huyet va có nong dO dtrang huyet gan trong
ducmg vai 10 chirng am (ching am: 527,29
mg/di, tra Kombucha: 499.00 mg/dl).
Cac ket qua a 10 1, 2, 3 cho thiy co sir
thong &Ong vai cac nghien cfru truck do ye tac
dOng ha throng huyet cua trai , dau bap (Tra
(Trap
Hoang Dung, HuS/nh Xuan Yen (2012)), dm
hat methi (Nawel Hamza VA cs (2012)) va ctia
re cay hoang lien (Lao Dire Thuan va cs
(2013))1I][311101. Tuy nhien, ngupc lai
nghien cfru cua Thummala Srihari va cs
(2013), tac dOng cua tra Kombucha tren mo
hinh chuOt tier throng cua chung toi khong
cho ket qua ha dtremg huyet1141.


15

TAP CHI KHOA HOC TRUONG DAI HOC MO TP.HCM - So 4 (37) 2014

Nhu vay, viec sir dung cao con chiet cua
hat dau bap, hat methi va re cay hoang lien deu
có tac dung ha duiyng huyet tren mo hinh cua
chung toi. Tuy nhien, tra Kombucha khong co
tac dung nay. De kiem tra tic deng cua cac

loai cao chiet va tra Kombucha tren chuet,
kiern tra cacs chi so . ve
Chung toi tien
tong hugng hong cau va tng
trong luting,, tong
lu ng bach cau.
ti

3.3. Chi lieu troll.? Item
Bang 3. Trong lucnig cua chuiit (g) qua cac ngay thi nghiem o cac to kink nhau
NO

N5

N1O

N15

N20

L8 del ching am

19,36 ± 0,65

22,36 ± 0,83

22.90 ± 0,50

23,06 ± 1,47

24,13 ± 1,10

LO deli chting throng

20,41 ± 0,77

21,27 ± 0,81

22,42 ± 0,58

23,90 ± 1,49

24,44 ± 1,10

Lo 1 (Nu bip)

20,22 ± 0,32

20,93 ± 0,27

21,89 ± 0,46

22,10 ± 0.49

22,94 ± 0,46

Lo 2 (Hat methi)

19,39 ± 0,31

21,21 ± 0,55

21,85 ± 0,59

22,98 ± 0,76

24,95 ±1,39

L8 3 (Re holing lien)

20,41 ± 0,23

21,46 ± 0,28

22,45 ± 0,28

23,39 ± 0,23

24,19 ± 0,23

L8 4 (Kombucha)

20,16 ± 0,22

21,58 ± 0,19

23,01 ± 0,17

23,98 ± 0.13

24,82 ± 0,01

Hinh 2. DO thi bieu dien sty bien thien trong luting cua at, eit thi nghii2m
30

25

0

NO

N5

NGAI"

NIO

NI5

N20

NGIIIEN1

Trong luting cua chuet la , met trong
nhang chi tieu dau tien can xet den khi danh
gia hieu qua tac deng cua vat lieu dtrov thi
nghiem tren chuet. Trong luting trung binh cua
chuet khi dua vao thi nghiem nam trong
khoang 20 gram. tai ngay 0. Sau 20 ngdy khdo

sat, trong luting trung binh cua chuet tang Ian
loot la 24,13; 24,44; 22,94; 24,95; 24,19;
24,82 g tucmg irng ran loot Or cac 10 dei chUng
am, doi chUng &rang, 10 sir dung cao chiet trai

au bap, cao chiet hat methi, cao chiet re
hoang lien va tra Kombucha. Trong luting cua
chuet co six tang len nhung khong CO khac biet
ye mat thong ke so vai ngay 0. Ngoai ra, trong
luting cua chuet a cac 10 khac nhau ding
khong co su khac biet ve mat thong ke. Dieu
nay chimg to viec sir dung cao chiet cac foal
thao dugc va tra Kombucha khong gay anh
huong den trong luting cua chuet thi nghiem.


16

CONG NGHE
3.4. Chi tieu hOng
) aim
Bang 4. Chi so hOng cau (x 10' 4 bao/mm) ciia chuOt qua cac ngay thi nghiem
NO

N5

N1O

N15

N20

1054,30 ± 16,75

1069,90 ± 14,76

1057,40 ± 10,16

1087,90 ± 16,67

1097,7 ± 18,60

Lo diii chirng dtrcrng 1078,40 ± 125,79

936,36 ± 24,24

837.86 ± 50,18

828,71 ± 41,54

746,14 ± 42,53

Lo 1 (Nu big)

1008,14 ± 30,48

828,00 ± 13,69

835,49 ± 11,72

852,70 1 15,25

849,51 = 20,91

L8 2 (Hat methi)

1096,70 ± 12,01

959,57 ± 8,577

851,86 ± 12,42

803,86 ± 12,94

770,71 ± 12,57

Lo 3 (Ile hoang lien) 1049,60 ± 13,84

952,00 ± 15,03

870,42 ± 12,75

806,57 ± 5,576

783,71 ± 4,098

1071,30 ± 13,77

1064,60 ± 14,31

1092,10 ± 13,24

1101,80 ± 21,14

1099,80 ± 27,35

Lo diii chtrng am

Lo 4 (Kombucha)

Hinh 3. DO thi bieu (lien sy bien thien chi so hong cau ciia chuet thi nghiem
1200
1000
800

DC AM
-III- DC DI:ONG

GOO

DAL' BAP

x

400
.

'IETHI
-HOANG LIEN

2tX)

--?,---KOMBUCHA

0
NO N5 N10 N15
'GAY T111 NCI-1101

Ket qua cho thay co six lien he gifra dai
than duang va tong lugng hong cau,cim chuOt
trong do sir tang len ve duemg huyet dan den
six glycate him hemoglobin gay ra thieu but
hemoglobin On yeti oxy. Hien tucmg nay can
den six tang len to bao hong cau de bo sung cho
lacing hemoglobin bi glycate hoa. Vao ngay 0,
lugng hong cau 6. tat ca cac lo thi nghiem deu
vuert ngu6ng gia tri gieri han binh thu6ng cua
chuOt la 5 - 9,5 x 106 t6 baohnm3171. Sau 20
ngay thi nghiem, chi so . hiing au 6 lo dOi
chirng khong co six thays doi trong khi lo chimg
duang c6 tong lucmg hOng cau giam ro ret va
nam trong ngifang sinh ly binh thlrerng cua

N20

chuOt (7,5 x 106 to bao/mm3).
Cac lo 1, 2, 3 CO tac dung ha tong so
hong cau tuong to lo doi chirng clucrng va deu
nam trong gi6i han sinh 1j, binh thireing trong
khi lo so 4 khorig có tic Flung nay. Ket qua nay

phii ,hop vai ket qua ye kha nang ha clueing
huyet cua cac loai thao duo.c (mac 3.2) va
nghien cau dm Lao Dirc Thua'n va cs (2013)131.
Dieu nay cho thay rang sau khi sir dung cac
loai cao chiet, chi so hong cau cua chuOt da
dugc on dinh va ram trong gi6i han sinh lY
binh thuerng.


TAP CHI KHOA HOC TRUONG DAI HOC MO TP.HCM - SO 4 (37) 2014

17

3.5. Chi tieu kick du
Bing 5. Chi se 4ch cau (te bao/mm3) cua chi* qua cac ngay thi nghiem
NO

N5

N10

N15

N20

6082,3 ± 567,0 6585,3 ± 666,5 7468,7 ± 2448,4

7590,1 ± 940,7

8235,0 ± 1411,0

Lo TM cluing throng 6127,1 ± 474,6 7302,6 ± 780,8 7943,7 ± 2003,6

8258,6 ± 630,9

8403,9 ± 634,3

LO 1 (Nu bip)

6151,0 ± 386,7 6437,1 ± 231,4

7238,6 ± 357,2

7932,7 ± 273,9

10434,3 ± 312,5

Le 2 (Hat methi)

6069,7 ± 31,5

6567,4 ± 51,3

7767,4 ± 54,8

8701,7 ± 81,1

9532,1 ± 315,4

Lo 3 (Re hoing lien)

6157,6 ± 77,5

7515,3 ± 45,5

7729,4 ± 47,5

7924,9 ± 140,1

8398,3 ± 52,4

Lo 4 (Kombucha)

6065,4 ± 7,3

6066,0 ± 12,7

6739,8 ± 176,6

6958,0 ± 144,0

7204,1 ± 20,5

Le dOi chting am

Hinh 4. DO thi bieu dien str bien thien tong se bach cau cua chuet thi nghiem
11000
10000
8000
•

DC AM
-III-

• •. 6000

()t

DAC BAP
4000

-NIETHI

•

-HOANG 1.111: \

2000

KONIBUCIIA

0

NO N5 N10 N15 N20
NGAY NG13101

Vao ngay 0, tong luting bach cau cua cac
16 thi nghi8m deu nam trong khoang sinh 1j/
binh thtrimg171. Sau 20 ngay, Kiang bach cau
cac 16 thi nghi8m c6 tang len nhung van nam

trong khoang gia tri sinh ly binh thuang (314,2 x 103 to bao/mm3). Su tang bach cau a
day c6 th8 do nhi6'm trong qua trinh tiem,va do
gia tri throng huyet. Ket qua nay cho thay cac
dang cao chiet thao dtrac va tra Kombucha
khong gay anh huang len 118 mien dich cua
chu6t thi nghi8m.
Dua tren cac k8t qua thu nhan dtro.c,
thong t6i nhan thay cao chi8t con trai dau bap
lieu 40 g/kg, cao chiet con hat methi lieu 30
g/kg va cao chi8t con r8 hoang lien lieu 150
mg/kg c6 kha nang ha &rang huyet a mo hinh

chu6t thi nghi8m. Ngoai ra, cac did° clugc sir
dung trong nghien ciru khong anh huang den
cac chi deli sinh ly binh thtrang cua chu6t.
4. Ket 1u4n
Tir cac ke't qua thi nghi8m dat dtrac, cao
chiet con trai au bap, hat methi, cay hoang
lien thir nghi8m tren mo hinh d6ng vat co tac
dung ha throng huyet sau 20 ngay sir dung a
cac li8u 40 g/kg, 30 g/kg va 150 mg/kg. Tuy
nhien, tra Kombucha khong c6, tac dung ha
dui:mg huyet a lieu 0,09 ml. Tat ca cac loaf
thao &rot sir dung trong nghien ciru a cac lieu
tren la an toan dOi vai chu6t thi ngshi8m the
hi8n qua trong luting, chi so hong cau va chi
so bach cau.


18

CONG NGHC
TAI LIEU THAM KHAO

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