Thành phần hóa học và hoạt tính sinh học của cao chiết từ lá loài dương đồng bốc (adinandra bockiana e pritz ẽ diels)thành phần hóa học và hoạt tính sinh học của cao chiết từ l

Khoa hoc Y - Dude I Duoc hoc

DOI: 10.31276/VJST.64( 12).34-38

Thanh phan hoa hocr va hoat tinh sinh hoc cua cao chiet
tir la loai Diro’ng dong boc (Adinandra bockiana E . Pritz. ex
x

N g u y en H u u Q uan*, N g u y en T h | N g o c L a n , N g u y en T hj T h u N g a , S y D an h T h u tm g , C h u H o a n g M au

K hoa Sinh hoc, T ruong D ai hoc S u p h a m Thai N guyen
N g a y n h an bai 2 0 /5 /2 0 2 1 ; n g a y c h u y e n p h an b ie n 2 4 /5 /2 0 2 1 ; n g a y nhan p han b ie n 2 4

6 2 0 2 1 : n g a y ch ap nhan d a n g 2 /7 /2 0 2 1

T om tat:
D u o n g d o n g h o c c o te n k h o a h o c la

A d in a n d ra b o ckia n a

E . P r it z . e x D ie ls , t h u o c c h i D u o n g d o n g p h a n b o o’ t in h L a o C a i, V ie t

N a m . M o t so lo a i t h u o c c h i D u o n g d o n g d a d u o c n g h ie n c u u v e d a c d ie m t h u c v a t v a c a c c h a t c o h o a t tin h s in h h o c t r o n g c a y , tu y
n h ie n c h o d e n n a y lo a i D u o n g d o n g h o c c h ir a d u o c ta c g ia n a o n g h ie n ciru . D o d o . n g h ie n c u u t h a n h p h a n h o a h o c v a h o a t tin h
s in h h o c c u a c a o c h ie t tir la lo a i n a y la c o n g tr in h d a u tie n v a c a n t h ie t t r o n g th ir c tie n . C a o c h ie t tir la c iia lo a i D u o n g d o n g h o c
c o c h u a n h o m c h a t p o ly p h e n o l, ta n in v a c o u m a r in . C a o e t h a n o l, d ic h lo r o m e t h a n v a e t h y l a c e t a t
lie c h e s u p h a t t r ie n c u a v i k h u a n

B. su b tilis , L. p la n ta r u m

tin h k h u g o c t y d o D P P H m a n h , g ia tr j E C

va

S. m a cescens.

0 n o n g d o 2 0 , 6 0 v a 2 0 0 p g /m l

C a o e t h a n o l, d ic h l o r o m e t h a n e v a e t h y l a c e t a t e c o h o a t

la n l u o t d a t 0 .2 , 0 ,4 7 v a 5 ,7 p g /m l. C a o e t h a n o l c o h o a t tin h u c c h e c a c d o n g te b a o

u n g th ir d a d a y , p h o i v a v u v o i g ia t r | I C 5(I la n l u o t la 5 8 .6 2 . 4 8 ,7 3 v a 4 3 ,1 5 p g /m l. K e t q u a n a y c h o th a y , lo a i D u o n g d o n g h o c la
c a v tr o n g tie m n a n g b o i c h u a n h ie u h o p c h a t c o k h a n a n g k h a n g k h u a n , k h a n g o x y h o a v a u c c h e c a c d o n g te b a o u n g t h u .

Tir kh o a :

c a o t o n g , c h o n g o x y h o a , D u o n g d o n g h o c . k h a n g k h u a n , k h a n g te b a o u n g t h u .

C h i so p h a n loai:

3 .4

Oat van fle

n g h ie n c u u n a y v a c u n g la n g h ie n c u u d a u t ie n v e lo a i D u o n g

V ie t N a m la q u o c g ia c o d a d a n g s in h h o c p h o n g p h u v a
tie m n a n g c a o v e c a y d u g c lie u . T u y n h ie n , c o n h ie u lo a i c h u a
d u g c n g h ie n c u u v e tarn q u a n tr g n g v a g ia tri la m th u o c c u a
c h u n g , d a c b ie t la c a c lo a i th u o c c h i D u o n g d o n g . 0 V ie t N a m ,

c h i D u o n g d o n g c o k h o a n g 1 4 lo a i, p h a n b o ra i r a c k h a p c a
n u o c [1 ] , L o a i

A d in a n d r a lie n ii

d o n g b o c d e tra lo i c a u h o i tren . N g h ie n c u u tie n h a n h ta c h c a o
c h ie t , d jn h tin h th a n h p h a n h o a h g c c u a lo a i D u o n g d o n g b o c
v a d a n h g ia h o a t tin h k h a n g k h u a n , c h o n g o x y h o a , k h a n g te
b a o u n g th u c u a c a o c h ie t th u d u g c .

Vat lieu va pluffing phap nghien ciiti

b u o c d a u d u g c n g h ie n c u n v e

V a t lie u va m o i tn r o n g m id i c a y

\ j tri p h a n b o d ia ly , d a c d ie m th irc v a t h p c v a tr in h t u g e n

m a tK ,

v u n g IT S g iu p n h a n d ie n lo a i [2 , 3 ] . N g o a i ra, th a n h

p h a n h o a h g c v a h o a t tin h k h a n g k h u a n tir la c u a lo a i

A . lie n ii

d u g c c h ir n g m in h c o h o a t tin h k h a n g k h u a n , c h o n g o x y h o a v a
r c c h e m o t s o d o n g te b a o u n g th u [4 ] , L a n T h i N g o c N g u y e n
■a c s ( 2 0 2 0 ) [5 ] d a n g h ie n c u u v e lo a i

A . m e g a p h y lla

H u va

n h a u t h a y c a o c h ie t tir la c u a lo a i n a y c o c h ii a c a c h g p c h a t
r*: %n h e n o l, c o u m a r in ; d o n g t h o i c h u n g m in h c a o c h ie t tir la c o
h : at tin h k h a n g k h u a n , k h u g o c t u d o v a u c c h e d o n g t e b a o u n g
•J- _• • u. d a d a y . p h o i. N h u v a y , c a c n g h ie n c u u a V ie t N a m h ie n
* -;■ t i p :r u n g c h u y e u v a o

A . lie n ii v a A. m e g a p h y lla

la c a c lo a i

L o a i D u o n g d o n g b o c s u d u n g tr o n g n g h ie n c u u d u g c th u
ta i K h u b a o to n th ie n n h ie n H o a n g L ie n - V a n B a n t h u o c x a
L ie r n P h u , h u y e n V a n B a n , tin h L a o C a i v a o th a n g 8 / 2 0 1 9 o d o
c a o 8 0 0 m , tg a d o 2 1 ° 5 9 ’ 1 5 ” N , 1 0 4 ° 1 9 ’2 8 ” E . T e n k h o a h g c
c u a lo a i d u g c x a c d jn h b o i P G S .T S S y D a n h T h u b n g , T r u o n g
D a i h o c S u p h a m , D a i h g c T h a i N g u y e n (h in h 1). L o a i D u o n g
d o n g b o c th u th a p d u g c s u c h o p h e p c u a B a n q u a n ly K h u b a o
to n th ie n n h ie n H o a n g L ie n - V a n B a n . M a u la d u g c sir d u n g d e
ta o c a o c h ie t v a n g h ie n c u u c a c b u o c tie p th e o .

- - i " be c h u y e u cr k h u v u c m ie n n u i p h ia B a c .
r d o n g b o c h a y c o n g o i la H o n g d a m T a m D a o , c o

t

- * o o a h v la

~ r :'" c
ii ci

A d in a n d ra b o c k ia n a E . P ritz. e x
A d in a n d ra ). h g C h e ( T h e a c e a e ).

r J r . g o . cao

n ac r - g _-_ r.g
A n e r«:c c rar. .

-.: i

i

(A)

c o n g trin h n g h ie n c u u n a o v e lo a i n a y . V a y lo a i
.

sf. r u

2-6 m v a t h u o n g m o c tr e n s u o n n u i

ang. n oi c o d o cao 2 0 0 -1 5 0 0 m . L oai D u o n g

p h i n b o o T r u n g Q u o c v a V ie t N a m . H ie n n a y ,

O ta u a g
i a

D ie ls , th u o c
D uong dong

j\.

fcrr c h a t f

ha> k h o n g c o h g p c h a t c o k h a n a n g k h a n g
-

a. k h a n g te b a o u n g th u v a n e u c o th i la
car rao

E>a>- c h in h la c a u h o i d a t ra c h o

5*TZ> 12-2022

(B)

Hinh 1. Hinh anh cua loai Duong d6ng boc sir dung trong
nghien ciru. (A) Mat sau cua la; (B) Canh mang nu (anh d u g c
chup beri nhom tac gia trong qua trinh thu mlu).

34


Khoa hoc Y - DitOc I D M c hoc

lu p n g k h o n g d o i, s a u d o d e m n g h ie n n h o . M a u n g h ie n d u p e

Chemical composition and biological
activity of leaf extracts
of Adinandra bockiana E. Pritz. ex Diels

n g a m c h ie t h a i la n b a n g e th a n o l tr o n g t h ie t b i s i e u a m

6 n h ie t d o

p h o n g . D ic h t o n g s o d u p e c a t k ie t d u n g m o i d u d i ap su a t g ia m ,
n h ie t d o < 5 0 ° C v a th u d u p e c a n c o e th a n o l. C a n c o e th a n o l d u p e
p h a lo a n g b a n g n u d e c a t, s a u d o c h ie t p h a n b o la n lu p t v d i c a c
d u n g m o i la d ic h lo m e t h a n v a e th y l a c e ta t. S a u k h i c a t d u o i d u n g

Huu Quan Nguyen*, Thi Ngoc Lan Nguyen,
Thi Thu Nga Nguyen, Danh Thuong Sy, Hoang Mau Chu

m o i th u d u p e c a n d je h d ic h lo m e th a n , e t h y l a c e ta t v a c a n c h ie t.
C a c c a n d ic h th u d u p e s e s a y k h o d n h ie t d o 5 0 ° C v a th u d u p e

D ep artm ent o f Biology, Thai N guyen U niversity o f E ducation

c a o s a y k h o t u o n g u n g . C a c c a o c h ie t d u p e c h o v a o b in h th u y
tin h , g h i n h a n , d a y k in v a b a o q u a n d n h ie t d o t h u d n g d e s u

R e c e iv e d 2 0 M a y 2 0 2 1 ; a c c e p te d 2 J u ly 2 0 2 1

d u n g tr o n g c a c n g h ie n c u u tie p th e o .

Abstract:

Dinh tinh cac hap chat co trong cao ethanol

Adinandra bockiana E. Pritz. ex Diels collected in Lao Cai
province, Vietnam belongs to the Adinandra genus. Some
species of the genus Adinandra have been studied for their
botanical characteristics and bioactive compounds, however,
A. bockiana has not been studied to date. Therefore, studying
the chemical composition and biological activity of A.
bockiana leaf extracts is pioneering and necessary in reality.
The leaf extracts of A. bockiana have been quantified and
determined to contain tannins, coumarins and poiyphenolic
compounds. The ethanol extract, dichloromethane extract and
ethyl acetate extract at the concentrations 20,60, and 200 pg/ml
can inhibit the growth of B. subtilis, L. plantarum, and
S. marcescens. The ethanol extract, dichloromethane extract
and ethyl acetate extract have free radical reduction activity,
which EC50values reached 0.2,0.47, and 5.7 jug/ml, respectively.
The ethanol extract exhibited inhibitory activities on gastric,
lung, and breast cancer cell lines with IC50 values of 58.62,
48.73, and 43.15 pg/ml, respectively. The results show that A.
bockiana is a potential plant that contains many compounds
that have antibacterial, antioxidant, and anticancer activities.

D in h tinh p o ly p h e n o l: P h a n u n g v d i m u o i s a t (I I I ), la y 5 m l c a o
e th a n o l c h o v a o 2 o n g n g h ie m k y h ie u la n lu p t la I v a II. C h o th e m
0 ,5 m l m u o i sa t (I I I ) v a o o n g II v a q u a n sa t h ie n tu p n g . T u y t h e o s o
lu p n g v a v i tri n h o m h y d r o x y l tr o n g p h a n t u p o ly p h e n o l m a c h o
m a u lu c , x a n h h o a c n a u . P h a n u n g v d i d u n g d ic h H , S 0 4 d a c , la y
2 m l c a o e th a n o l v a o 2 o n g n g h ie m k y h ie u la n lu p t la I v a II.

C h o t h e m 1 -2 g i o t H 2S 0

4 d a c v a o o n g II v a q u a n sa t h ie n tu p n g .

K h i n h o H 2S 0 4d a c le n c a c d a n x u a t c u a fla v o n v a f la v o n o l th i
c h o m a u v a n g d a m ; d o i v d i c h a lc o n v a a u r o n c h o m a u d o , d o
th a m v a d o t u a i; fla v a n o n c h o m a u d o d a c a m d o s u c h u y e n
th a n h c h a lc o n .

D in h tinh ca c c o u m a rin :

L a y 2 m l c a o e th a n o l v a o 2 o n g

n g h ie m k y h ie u la n lu p t la I v a II. C h o v a o o n g II 0 ,5 m l d u n g
d ic h N a O H 10% . D u n c a 2 o n g tr e n b e p e a c h t h u y d e n s o i, la y
ra d e n g u o i, sa u d o c h o th e m 4 m l n u d e c a t v a o c a 2 o n g I v a
II. Q u a n sa t th a y c h a t lo n g d o n g II ( c o k ie m ) trd n e n tr o n g s u o t
h o a c tr o n g h o n o n g I ( k h o n g k ie m ) c o th e x e m la c o c o u m a r in .
N e u d e m a x it h o a o n g n g h ie m c o k ie m b a n g m o t v a i g i o t H C1
d a c m a la m c h o d ic h m a t m a u v a n g d u e h o a c x u a t h ie n k e t

Keywords: Adinandra bockiana E. Pritz. ex Diels, antibacterial,

tu a b o n g ( c u n g c o k h i x u a t h ie n k e t tu a ) th i c o th e k e t lu a n c o

anti-cancer, antioxidant, total leaf extract.

c o u m a r in [ 6],

D in h tin h tannin:

Classification number: 3.4

L a y 2 m l c a o e th a n o l v a o 2 o n g n g h ie m k y

h ie u la n lu p t la I v a II. C h o t h e m th u d e th u v a n ilin /H 2S 0 4v a o
o n g I la c d e u v a q u a n sa t. N e u d ic h c h ie t c h u y e n s a n g m a u d o
C a c lo a i v i k h u a n k ie m d in h

L. p la n ta n u m

B. su b tilis, S. m a rce sc en s

d a m la c h u n g to c o ta n in .
va

Phieang phap thw hoat tmh khang khuan cua cao chiet

d o K h o a S in h h o c , T r u o n g D a i h o c S u p h a m , D a i

h o c T h a i N g u y e n c u n g c a p . M o i tr u d n g L B d e n u o i c i y v i k h u in

H o a t tm h k h a n g k h u a n c u a c a o c h ie t t u la c u a lo a i D u o n g

k ie m d jn h c o th a n h p h a n g o m c a o n a m m e n 0 ,5 % , N a C l 1 ,0% ,

theo
djnh
d u p e n u o i h o a t h o a tr o n g m o i tr u d n g L B lo n g d 2 8 ° C , la c 200
v o n g /p h u t tr o n g th d i g ia n 8h . H u t 7 0 p i d ic h n u o i m o i loai vi

k h u a n d a d u p e h o a t h o a le n d ia m o i tr u d n g L B d a c va trai deu
tr e n m a t th a c h c h o d e n k h i k h o . D u e c a c g ie n g c o duemg kinh 10
m m tr e n d ia th a c h v a n h o 100 p i c a o c h ie t m o i lo a i tu la cua loai
D u o n g d o n g b o c v a o g i e n g d c a c n o n g d o 2 0 , 60 va 200 pg ml
( g i e n g d o i c h u n g b o s u n g D im e t h y l s u l f o x id e (DMSO). Datcac
d ia p e tr i d a b o s u n g d ic h v a o tu la n h 4 ° C khoang 1-2 gjo roi d |i
v a o tu a m n u o i d 3 0 ° C , t u 18 d e n 2 4 gid. Do duong kiah
k h a n g k h u a n , c h u p h in h v a g h i la i ket qua. Moi t h i n g h a e a id ta Q E

p e p to n 1 ,0 % . M o i tr u d n g L B d a c b o s u n g 2 ,0 % th a c h agar.
C a c d o n g t e b a o u n g th u g d m : M D A - M B - 2 3 1 ( u n g th u v u ) ,
A G S (u n g th u d a d a y ) v a A 5 4 9 (u n g th u p h o i) d u p e s u d u n g d e
x a c d jn h h o a t tin h g a y d o c te b a o d o P h o n g T h u n g h ie m s in h
h o c , V ie n C o n g n g h e S in h h o c , V ie n H a n la m K H & C N V ie t
N a m c u n g cap.

Phvffng phap ctieu che mau thic hoat tinh
L a c u a lo a i D u o n g d o n g h o c th u h a i d u p e r u a s a c h v a th a i
n h o , p h o i tr o n g b o n g m a t , s a y k h o

KHOA HOC
CONG

6 n h ie t d o 5 0 ° C d e n k h o i

64(12) 12.2022

d o n g b o c d u p e x a c d in h b a n g p h u o n g p h a p d u e lo th a c h
B . M a h e s h v a S . S a t is h ( 2 0 0 8 ) [7 ] . C a c lo a i v i k h u a n k ie m

35


Khoa hoc Y - DiiOc I Dupe hoc

la p la i 3 la n . D u o n g k ln h v o n g k h a n g k h u a n d u g c x a c d in h th e o

C a 4 c h a t D P P H . E llip t ic in e . D M S O , S R B d u g c c h u n g

c o n g th u c: H = D - d (m m ). T r o n g d o : D la d u d n g k ln h v o n g

to i sir d u n g la c h a t tin h k h ie t, d u g c c u n g c a p tir H a n g S ig m a

k h a n g k h u a n (m m ); d la d u d n g k ln h g ie n g th a c h (m m ).

A ld r ic h v a sir d u n g la m c h a t c h u a n , c u n g n h u d e p h a c a o c h ie t.

P h ir m g p h a p x a c d in h k h a n a n g c h o n g o x y h o a D P P H

Ket qua

K h a n a n g c h o n g o x y h o a c u a c a o c h ie t tir la c u a lo a i D u c m g
d o n g h o c d u g c x a c d in h t h e o p h u o n g p h a p c u a J. T ab art v a c s
( 2 0 0 9 ) [ 8] sir d u n g k h a n a n g tru n g h o a g o c tu d o D P P H . 1 0 0 p i
c a o c h ie t m o i lo a i d c a c n o n g d o 0 ,5 , 2 ,

D in h tin h c a c h o p c h a t co tr o n g la c u a lo a i D u o n g d o n g
boc
D in h tin h p o ly p h e n o l:

8, 3 2 v a 6 4 p g /m l d u g c

C a o e th a n o l tir la c u a lo a i D u o n g

b o s u n g 2 ,9 m l d u n g d je h D P P H n o n g d o 0 ,1 m M p h a tr o n g

d o n g b o c d u g c d jn h tin h b a n g th u o c thir v d i m u o i sa t (I II),

d u n g d ic h m e t h a n o l, la c d e u v a d e y e n tr o n g 3 0 p h u t, sa u d o d o

n h a n t h a y d u n g d ic h tr o n g o n g n g h ie m II c h u y e n s a n g m a u

o b u d c s o n g 5 1 7 n m . K h a n a n g k h ir g o c t u d o D P P H d u p e tin h

x a n h lu c v a k h a c s o v d i m a u c u a o n g n g h ie m I (h in h 2 A ) .

th e o c o n g thvrc sau :

K h i d in h tin h b a n g H , S 0 4 d a c , d u n g d ic h tr o n g o n g n g h ie m

K h a n a n g k h ir g o c t u d o D P P H (% ) = 1 0 0 x ( A c - A s) / A c.

II c h u y e n s a n g m a u n a u d a m h o n m a u c u a o n g n g h ie m I (h in h
2 B ) . N h u \ a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o c h iia n h o m

tr o n g d o : A c la d o h a p th u q u a n g c u a m a u d o i c h im g ; A s la d o h a p

c h a t p o ly p h e n o l .

th u q u a n g c u a m a u c a n x a c d in h . K h a n a n g c h o n g o x y h o a d u g c

D in h tin h c a c tan n in :

x a c d in h d u a v a o g ia tri E C 50 ( la n o n g d o m a u c o k h a n a n g k h u

C a o e th a n o l tir la c u a lo a i D u o n g

g o c t u d o D P P H d a t 5 0 % ).

d o n g b o c d u g c d jn h tin h b a n g th u o c thir v a n il in tr o n g H 2S 0

P h ir m g p h a p x a c d in h tin h d o c te bao u n g th ir n u d i ca y
d a n g d m lo p

m a u c u a o n g n g h ie m I (h in h 2 C ) . N h u v a y , tr o n g la c u a lo a i

T h u d o d o c te b a o u n g th u

in vitro

la m c h o d u n g d je h tr o n g o n g n g h ie m II c o m a u d o k h a c s o v d i

D u o n g d o n g b o c c o c h u a n h o m c h a t ta n in .

d u g c x a c d jn h th e o

D in h tin h c a c c o u m a rin :

p h u o n g p h a p c u a A . M o n k s v a c s ( 1 9 9 1 ) [9 ]. C a o c h ie t tu la c u a
lo a i D u o n g d o n g b o c d u g c c h u a n bj v a th u n g h ie m d 4 n o n g

d o 1 0 0 , 2 0 , 4 v a 0 ,8 m g /m l. C h a t th a m k h a o E llip t ic in e d c a c
n o n g d o 1 0 , 2 , 0 ,4 v a 0 ,0 8 p g /m l d u g c sir d u n g n h u la c h a t d o i
c h u n g d u o n g . D M S O d n o n g d o 10% d u g c sir d u n g n h u la c h a t
d o i c h u n g a m . H a m l u g n g p r o te in t o n g s o c u a te b a o d u g c x a c

4

K e t q u a h in h 2 D n h a n th a y , k h i

c h o 0 ,5 m l N a O H 10% v a o o n g n g h ie m II, d e m d u n c a 2 o n g
n g h ie m tr e n b e p e a c h th u y , s a u k h i la m n g u o i v a c h o th e m
4 m l n u d e c a t v a o c a h a i o n g th i d u n g d je h o n g II trd n e n tr o n g
h o n . S a u k h i c h o v a i g ig t d u n g d je h HC1 d a c v a o c a h a i o n g
n g h ie m th i o n g II m a t m a u v a n g d u e ( h in h 2 E ) . T ir k e t q u a d jn h

d in h d u a v a o m a t d o q u a n g h g c ( O D ) d o d u g c k h i th a n h p h a n

tin h c h o th a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o ch ira n h o m

p r o te in c u a te b a o d u g c n h u d m b a n g S u lf o r h o d a m in e B ( S R B ) .

c o u m a r in . C a c n h o m h g p c h a t p o ly p h e n o l , ta n n in v a c o u m a r in

G ia tri O D d u g c d g c d b u d e s o n g 5 1 5 n m tren m a y E L I S A P la te

c o y n g h la tr o n g v i e c irn g d u n g la m th u o c c h u a b e n h v a k e t

R ea d er. G ia tri O D m a y d o d u g c t y le th u a n v d i l u g n g S R B g a n

q u a n a y la c o s o d e t ie n h a n h c a c n g h ie n c u u t ie p t h e o v e lo a i

v d i p h a n t u p r o te in . G ia trj O D c a n g ld n th i h a m l u g n g t e b a o v a

D u o n g d on g boc.

h a m lu g n g p r o te in c a n g n h ie u . K h a n a n g g a y d o c te b a o d u g c

H o a t tin h k h a n g k h u a n c u a c a o c h ie t tir la c u a lo a i
D ir m g d o n g bde

x a c d in h b a n g n o n g d o c u a c h a t ire c h e 5 0 % s u p h a t tr ie n cira te
b a o . N d n g d o u c c h e 5 0 % te b a o (I C 50) d u g c x a c d in h la n o n g d o

H o a t tin h k h a n g k h u a n c u a c a o e t h a n o l, e t h y l a c e ta t v a

c u a m a u ire c h e d u g c 5 0 % te b a o , c a c g o c t u d o h o a c e n z y m e .

C hat c h u a n d u g c c o i c o h o a t tin h to t k h i IC 50= 5 p M [1 0 ] .

_s

d ic h lo r o m e t h a n tir la c u a lo a i D u o n g d o n g b o c d u g c tr in h b a y

(B)
(C)
(E)
(D )
: n polyphenol (A, B), tannin (C) va coumarin (D, E) trong cao chiet ethanol tir la cua loai Diro’ng dong boc.
■
(B) Phan irng vdi dung djeh H2S 0 4 dac; (C) Phan ung vdi dung dich valin/H2S 0 4; (D) Phan img vdi NaOH;

prr- —I ia-r

W

:ao ethanol toan ph§n ban dau; II: cao ethanol sau phan ung.

M(12) *2-2022

36


Khoa hoc Y - Duo'c I DUOc hoc

la p la i 3 la n . D u o n g k ln h v o n g k h a n g k h u a n d u p e x a c d jn h th e o

C a 4 c h a t D P P H . E llip t ic in e . D M S O , S R B d u p e c h u n g

c o n g th u c : H = D - d (m m ). T r o n g d o : D la d u d n g k ln h v o n g

t o i sir d u n g la c h a t tin h k h ie t . d u p e c u n g c a p tir H a n g S ig m a

k h a n g k h u a n (m m ); d la d u d n g k ln h g ie n g th a c h (m m ).

A ld r ic h v a sir d u n g la m c h a t c h u a n . c u n g n h u d e p h a c a o c h ie t.

P h ic ffn g p h a p x a c d in h k lia n a n g c h o n g o x y h o a D P P H

Ket qua

K h a n a n g c h o n g o x y h o a c u a c a o c h ie t tir la c u a lo a i D u o n g
d o n g h o c d u p e x a c d in h t h e o p h u o n g p h a p c u a J. T ab art v a c s
( 2 0 0 9 ) [ 8] sir d u n g k h a n a n g tr u n g h o a g o c tu d o D P P H . 1 0 0 p i
c a o c h ie t m d i lo a i d c a c n o n g d o 0 ,5 , 2 ,

D in h tin h e d e h o p c h a t co tr o n g la c u a lo a i D u o n g d o n g
boc
D in h tin h p o ly p h e n o l:

8, 3 2 v a 6 4 p g /m l d u p e

C a o e th a n o l tir la c u a lo a i D u o n g

b o s u n g 2 ,9 m l d u n g d ic h D P P H n o n g d o 0 ,1 m M p h a tr o n g

d o n g b o c d u p e d in h tin h b a n g th u o c th u v d i m u o i s a t (I II),

d u n g d ic h m e t h a n o l, la c d e u v a d e y e n tr o n g 3 0 p h u t, sa u d o d o

n h a n t h a y d u n g d je h tr o n g o n g n g h ie m II c h u y e n s a n g m a u

d b u d c s o n g 5 1 7 n m . K h a n a n g k h ir g o c tir d o D P P H d u p e tin h

x a n h lu c v a k h a c s o v d i m a u c u a o n g n g h ie m I (h in h 2 A ) .

th e o c o n g t h u c sau :

K h i d in h tin h b a n g H 2S 0

K h a n a n g k h ir g o c t u d o D P P H (% ) = 1 0 0 x ( A c - A s) / A c.

4 d a c , d u n g d ic h tr o n g o n g n g h ie m

II c h u y e n s a n g m a u n a u d a m h a n m a u c u a o n g n g h ie m I (h in h
2 B ) . N h u v a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o c h u a n h o m

tr o n g d o : A c la d o h a p th u q u a n g c u a m a u d o i c h u n g ; A s la d o h a p

c h a t p o ly p h e n o l.

th u q u a n g c u a m a u c a n x a c d in h . K h a n a n g c h o n g o x y h o a d u p e

D in h tin h c a c ta n n in :

x a c d in h d ira v a o g ia tri E C 50 ( la n o n g d o m a u c o k h a n a n g khir

C a o e th a n o l tir la c iia lo a i D u o n g

d o n g b o c d u p e d jn h tin h b a n g th u o c thir v a n ilin tr o n g H , S 0 4

g o c tir d o D P P H d a t 5 0 % ).

P h u c m g p h a p x a c d in h tin h d o c te b a o u n g th u ■n u d i cay
d a n g d ffn l&p
T h u d o d o c te b a o u n g th u

in vitro

la m c h o d u n g d je h tr o n g o n g n g h ie m II c o m a u d o k h a c s o v d i
m a u c u a o n g n g h ie m I (h in h 2 C ) . N h u v a y , tr o n g la c u a lo a i

D u o n g d o n g b o c c o ch ira n h o m c h a t ta n in .

d u p e x a c d jn h th e o

p h u o n g p h a p c u a A . M o n k s v a c s ( 1 9 9 1 ) [9 ] . C a o c h ie t t u la c u a
lo a i D u o n g d o n g b o c d u p e c h u a n b i v a th u n g h ie m d 4 n o n g
d o 1 0 0 , 2 0 , 4 v a 0 ,8 m g /m l. C h a t th a m k h a o E llip t ic in e d c a c
n o n g d o 1 0 , 2 , 0 ,4 v a 0 ,0 8 p g /m l d u p e sir d u n g n h u la c h a t d o i
ch irn g d u o n g . D M S O d n o n g d o 10% d u p e s u d u n g n h u la c h a t
d o i c h u n g a m . H a m lu p n g p r o te in to n g s o c u a te b a o d u p e x a c

D in h tin h c a c c o u m a rin :

K e t q u a h in h 2 D n h a n th a y , k h i

c h o 0 ,5 m l N a O H 10% v a o o n g n g h ie m II, d e m d u n c a 2 o n g
n g h ie m tr e n b e p e a c h th u y , sa u k h i la m n g u o i v a c h o th e m
4 m l n u d e c a t v a o c a h a i o n g th i d u n g d je h o n g II tr d n e n tr o n g
h o n . S a u k h i c h o v a i g i o t d u n g d je h H C 1 d a c v a o c a h a i o n g
n g h ie m th i o n g II m a t m a u v a n g d u e (h in h 2 E ) . T ir k e t q u a d jn h

d in h d u a v a o m a t d o q u a n g h o c ( O D ) d o d u p e k h i th a n h p h a n

tin h c h o th a y , tr o n g la c u a lo a i D u o n g d o n g b o c c o c h u a n h o m

p r o te in c u a te b a o d u p e n h u o m b a n g S u lf o r h o d a m in e B ( S R B ) .

c o u m a r in . C a c n h o m h o p c h a t p o ly p h e n o l , ta n n in v a c o u m a r in

G ia tri O D d u p e d o e d b u d c s o n g 5 1 5 n m tr e n m a y E L I S A P la te

c o y n g h la tr o n g v i e c irn g d u n g la m th u o c c h u a b e n h v a k e t

R ea d er. G ia tri O D m a y d o d u p e ty le th u a n v d i lu p n g S R B g a n

q u a n a y la c o s o d e tie n h a n h c a c n g h ie n c u u tie p t h e o v e lo a i

v d i p h a n tir p r o te in . G ia tri O D c a n g ld n th i h a m lu p n g te b a o v a

D u o n g d o n g boc.

h a m lu p n g p r o te in c a n g n h ie u . K h a n a n g g a y d o c te b a o d u p e

H o a t tin h k h a n g k h u a n c u a c a o c h ie f tic la c u a lo a i
D u o n g d o n g boc

x a c d in h b a n g n o n g d o c u a c h a t ire c h e 5 0 % sir p h a t tr ie n c u a te
b a o . N o n g d o tic c h e 5 0 % te b a o (I C 50) d u p e x a c d jn h la n o n g d o

H o a t tin h k h a n g k h u a n c u a c a o e th a n o l, e t h y l a c e ta t v a

c u a m a u ire c h e d u p e 5 0 % te b a o , c a c g o c t u d o h o a c e n z y m e .

d ic h lo r o m e t h a n tir la c u a lo a i D u o n g d o n g b o c d u p e trin h b a y

C h a t c h u a n d u p e c o i c o h o a t tin h to t k h i IC 50= 5 p M [1 0 ] .

,A >

<B>

(C)

(D)

(E)

------ 2 K e t aua dinh tinh polyphenol (A, B), tannin (C) va coumarin (D, E) trong cao chiet ethanol tip la cua loai Du’O’ng dong boc.
z- s r - - z
E

5r

j -'Z

.?

—-:i sat III: (B) Phan dng vdi dung dich H2S 0 4 dac; (C) Phan dng vdi dung dich valin/H2S 0 4; (D) Phan dng vdi NaOH;

. y -»C dac: I cao ethanol toan phSn ban dSu; II: cao ethanol sau phan dng.

W -12) 12-2022

36


Khoa hoc Y - Duoc I DUOc hoc

M o t s o h o p c h a t d i v d n g c h u a c o u m a r in c o lie n q u a n d e n c a c d a c

L0I CAMON

tin h n h u c h o n g v ie m [ 1 2 ], k h a n g k h u a n [ 1 3 ] , k h a n g v ir u s [ 14] v a
c h o n g u n g th u [ 1 5 ] . N g o a i ra, c o u m a r in c u n g th e h ie n ta c d u n g
g a y d o c te b a o u n g th u d o i v b i c a c t e b a o H e p 2 ( l o a i te b a o b ie u

N g h ie n ciru n a y d u p e tai trp b b i Q u y p h at trien K H & C N q u o c
g ia (N A F O S T E D ) th o n g q u a d e tai m a so 1 0 6 .0 2 - 2 0 1 8 .3 3 8 . C ac
ta c g ia x in ch a n th an h c a m o n .

m o n g ir b i), a n h h ir b n g tb i q u a trin h a p o p t o s is , sir b ie n m d t c u a
lo p m a n g v i n h u n g m a o , la m g ia m h a m lu o n g h u y e t tucrng c u a

TAI LIEUTHAMKHAO

te b a o c h a t v a sir p h a n h u y n h a n [ 1 6 ] . C a o c h ie t t u la c u a lo a i

[1] Pham Hoang Ho (1999),
Publishing.

(A. lie n ii)

S u m lie n

c o ch ira n h o m c h a t p o ly p h e n o l , f l a v o n o id

v a c o u m a r in . C a o e th a n o l, e t h y l a c U a t v a d ic h lo r o m e t h a n utc

S ta p h y lo c o c c u s a u re u s,
B. su b tilis, S. m a rce ssen s, S a rc in a lu tea , L. p la n ta r u m v a E.

c o li a n o n g d o 2 0 0 p g /m l. T r o n g d o , c a o d ic h lo r o m e t h a n c o
c h e sir p h a t tr ie n c u a c a c lo a i v i k h u a n

h o a t tin h k h a n g k h u a n t o t h o n s o v b i c a o e th a n o l v a e th y l
a c e ta t [4 ] . C a o c h ie t tir la c u a lo a i S u m la Io n

(A. m e g a p h y lla

H u ) c h u a c a c h o p c h a t p o ly p h e n o l , c o u m a r in ; d o n g th b i c a o
e th a n o l, e t h y l a c e ta t v a d ic h lo r o m e t h a n c o h o a t tln h u c c h e
c a c lo a i v i k h u a n

L. p la n ta r u m

va

S. a u re u s, B. su b tilis, S. m a rce ssen s, S. lu tea ,
E. co li. C a o e t h y l a c e ta t v a e th a n o l c o h o a t

tin h k h u g o c t u d o D P P H , g i a tri E C 50 la n lu o t d a t 3 0 ,3 v a
3 3 ,2

p g /m l.

C ao

d ic h lo r o m e t h a n e

co

h o a t tin h

khu

goc

t u d o rat y e u , g ia tri E C 50> 1 2 8 p g /m l. C a o e th a n o l u c c h e

A n I llu s tr a te F lo r a o f V ietn a m ,

Young

[2] Nguyen Huu Quan. Kieu Thi Tra Giang (2019), “Use of matK DNA
barcode to identify A d in a n d r a samples collected at Lao Cai, Vietnam”, T N U
J o u r n a l o f S c ie n c e a n d T e c h n o lo g y, 197(4), pp.205-210.
[3] Huu Quan Nguyen, Phuong Dung Le, Danh Thuong Sy, Quang
Tan Tu, Hoang Mau Chu (2019), “Studying of anatomical characteristics
and sequence of I T S gene from A d in a n d r a lie n ii” , C A S E A N -6 P r o c e e d in g s ,
pp. 153-159.
[4] Nguyen Huu Quan, Than Thi Kim Phuong, Chu Hoang Mau (2019),
“Study on chemical composition and antibacteria activity of extracts total
from leaves of A d ia n d r a lie n ii species”, P r o c e e d in g s N a tio n a l B io te c h n o lo g y
C o n fe r e n c e 2 0 1 9 , pp. 178-182.
[5] Lan Thi Ngoc Nguyen va cs (2020), “Antibacterial, antioxidant and
anti - Cancerous activities of A d ia n d r a m e g a p h y lla Hu leaf extracts”, B io sc.
B io te c h . R es. C o m m ., 13(3), pp. 1015-1020.

3 d o n g te b a o u n g th u d a d a y , u n g th u p h o i v a u n g th u g a n
v o i g ia tri IC 50 la n l u o t la 6 7 ,7 6 , 7 7 ,0 2 v a 8 4 ,4 6 p g /m l [5 ].
T r o n g n g h ie n c u u n a y , c a o e th a n o l t u la c u a lo a i D u o n g d o n g

b o c c o c h u a c a c n h o m c h a t p o ly p h e n o l , ta n in v a c o u m a r in . C a o
e th a n o l, d ic h lo r o m e th a n v a e th y l a c e ta t u c c h e sir p h a t tr ie n c u a
vi khuan

B. su b tilis, L. p la n ta r u m

va

S. m a rce sc en s.

C a o e th y l

a c e ta t, d ic h lo r o m e t h a n v a e th a n o l c o h o a t tin h k h u g o c t u d o
D P P H m a n h , g ia tri E C

la n l u o t la 0 , 2 , 0 , 4 7 v a 5 ,7 p g /m l. C a o

e th a n o l u c c h e 3 d o n g t e b a o u n g th u v u , u n g th u d a d a y v a u n g
th u p h o i, g ia tri IC 50 la n lu o t d a t 4 3 ,1 5 , 5 8 ,6 2 v a 4 8 ,7 3 p g /m l.
N h u v a y , n g h ie n c u u n a y p h u h o p v e th a n h p h a n v a h o a t tin h

[6] Nguyen Thai An, Bui The Hung (2008), “Study on Flavonoid and
Coumarin components of drugs in dispersion method”, J o u r n a l o f P h a r m a c y ,
368, pp.37-40.
[7] B . Mahesh, S. Satish (2008), “Antimicrobial activity of some important
medicinal plant against plant and human pathogens”, W o rld J. A g ric . S c i., 4(S),
pp.839-843.
[8] J. Tabart, et al. (2009), “Comparative antioxidant capacities of phenolic
compounds measured by various tests”, F o o d C h e m ., 113(4), pp. 1226-1233.
[9] A. Monks, et al. (1991), “Feasibility of a high-flux anticancer drug

screen using a diverse panel of cultured human tumor cell lines”, J o u r n a l o f
th e N a tio n a l C a n c e r I n s titu te , 83(11), pp.757-766.
[10] J.P. Hughes, et al. (2011), “Principles of early drug discovery”, Br.
162(6), pp. 1239-1249.

>;nh h o c v b i c a c n g h ie n c u u d a c o n g b o . T u y n h ie n , h o a t tin h
k h a n g k h u a n , h o a t tin h khut g o c t u d o v a u c c h e c a c d o n g t ! b a o
- " g th u c u a c a o c h ie t tir lo a i D u o n g d o n g b o c tr o n g n g h ie n c u u
n i> m a n h h o n s o v b i lo a i S u m lie n v a S u m la Ion .

le t luan
N g h ie n c u u d a c h u n g m in h d u o c c a o c h ie t t u la c u a lo a i
-

200 p g /m l c a o e t h a n o l, d ic h lo r o m e t h a n v a

B. su b tilis,
m \ i S m a rcescen s. C a o e th y l a c e ta t , d ic h lo r o m e th a n

y i a c e ta l d e u ire c h e s u p h a t tr ie n c u a v i k h u a n

—

.

- ~

•t .
••*
■ ■a.

[11] H. Gao, et al. (2010), “Anti-proliferative elfect of camellianin A in
leaves and its apoptotic induction in human hep G2 and
MCF-7 Cells”, M o le c u le s , 15(6), pp.3878-3886.

A d in a n d r a n itid a

[12] R. El-Haggar, R.I. Al-Wabli (2015), “Anti-Inflammatory screening
and molecular modeling of some novel coumarin derivatives”, M o le c u le s ,
20(4), pp.5374-5391.

M n g b o c c o ch ira h o p c h a t p o l y p h e n o l, ta n in v a c o u m a r in .
"g do 2 0 .6 0 v a

.

J.

P h a r m a c o l.,

„
- '
'

-

h o a t tin h k h u g o c t u d o D P P H m a n h , g ia tri E C S0
1

a

\ a 5 ." u g m l. C a o c h ie t e th a n o l tir la c u a
-

c o h o a t tin h ire c h e 3 d o n g te b a o u n g th u

A s d a d a y v a u n g th u p h o i. g ia tri IC 50 la n l u o t d a t

- 5
“2 - -• ’ ■ -g ml. Ket qua nghien ciru da chirng
■■rfi Dw i_r i - . ~>x a loai thuc vat tiem nang chira cac chat
a* t a n * ' - na s x ■a hira hen phan lap dupe cac chat sach
m M n t 'in n
e

w

M 1 2 ) 12 2 022

[13] Y. Shi, C. Zhou (2011), “Synthesis and evaluation of a class of new
coumarin triazole derivatives as potential antimicrobial agents”, B io o r g . M ed .
C h em . L e tt., 21(3), pp.956-960.
[14] S.C. Tsay, et al. (2014), “Coumarins hinged directly on benzimidazoles
and their ribofuranosides to inhibit hepatitis C virus”, E u r o p e a n J o u r n a l o f
M e d ic in a l C h e m is tr y , 63, pp.290-298.
[15] Y. Jacquot, et al. (2007), “Synthesis, structure, and estrogenic activity
of 4-amino-3-(2-methylbenzyl)coumarins on human breast carcinoma cells”,
B io o rg . M ed . C h e m ., 15(6), pp.2269-2282.
[16] S. Mirunalini, et al. (2014), “Antiproliferative effect of coumarin by

modulating oxidant/antioxidant status and inducing apoptosis in Hep2 cells”,
B io m e d . A g in g P a th o ., 4(2), pp.131-135.

38