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
CONTENT
LIST OF TABLE

i


!"""#""$#%&'''''''''''''''''
()
!"#""*)#(&''''''''''''''''''
+,
)#" )#%&''''''''''''''''''-
$.''''''''''''/

ii

Faculty of Agronomy Hanoi University of Agricultural
Advance crop science program
Progress reports: SUBJECT ADVANCED RESEARCH PROGRAM 2010-2011
I. Introduction
,
0
       Tomato yellow leaf curl .  
1  2 )                      )
%)2 3     )    )    
45)*&20-
/,%))
4Bemisia tabaci)
  "  %)     
62%)74)52
)4)522


8,
.22)
      
4! 52 49! & 5
:49!: 562
&! ! & 
        2  
)*) 
2);2)
    8"";2! ()2
<
*&#%)
 07“ Investigating,study,

1

diogonistic Begomovirus virus on Tomato=
.2. Objectives and Requirements
6>7
#)%)
.?7
# 8))      (   
&
#*)%)
II. Literature review
2.1. Researches in the world.
2)%82""2@
;;,2;)
,)2)
#)))2)

2)2)

2.2. Host range
(  )    %)    2          8  
%)9!   )-+  )A,
4;;54!5%
!2!2!2!
9! )7B)2
$2$ )24&52>4*5
2.3. Symptoms

2

9#,
)
)
2
22)22
22)8)
2.4. Cause agent
8  """  8      1      48 5    
9! %))23
   20  "#+" *&      )
/""-)2#*&
7*&*&%2-""*&
)2*&%)
2.5. Vector and transmitation of Begomovirus
Bemisia tabaci 4(7 Aleyrodidae),     2  2  
2)2,$,88
  )2  )      -""              ))  2

82
2?)))CC2
#+#;28)
2)-D
;D"D.(
+"#-"E6212
!,2Bemisia tabaci ,)
1)))00
))2    +""    )          
0))72
)1

3

 )  9)   #) ))     
?)2)
))2
))))

)
III.Material and method
3.1. Location and duration of the study
#)(&.
#  . $ $)2(F 
)
3.2. Materials
#$7
#  !          4   (     
(2&*2$2()92(*)5
#  !  2  $.  2    ?2  #  )2    2

)2#
- .)G
%*&1
• %HIJB
• &6("J
3.3. Methodology

4

3.3.1. Field surveying
)2)
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
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5
3.3.2. Collecting and storing samples
#)7)
)7
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H!2
H
#)7)),
H*)7))#))

H)7#"
"

3.4 Research method
3.4.1. Survey methodology in prevalence and white fly density on the field
#8))7"&##/$$*

#CG)2))2"G)

#$: ))2"G
Ratio of disease

5

&
$4E5KLLLLLLLLLL1""
)
White fly density:
)
M4G5KLLLLLLLLLLL
)
3.4.2. DNA extraction
*&17
(i) )%
7
# )")"")
# )"%HIJB,-"

")
# :-"

"
7
# )"14775,)
# )"""G"2,)
4"+5
+7

# $"+14775
,)
#)",)
4"5

6

7
# $$,#"


"
# )",)2*&

2-7.BC"

4"2CG52B
,1B
C7*&+"
/7**&",#"


(ii) F)&6("J*&1
#")
#"&6("J
#*"#"""J2(K/
3.5. PCR test7
3.5.1. PCR testing:
N$.
#3%)*&#%). O%)4(2""-5

%). 7<#(J*(:%3
N
#+<
%)7<#393.3339.39.
N
#+<
N94G52.43G52(4GG52J4G52%4G3G52*4G3G52:43G5
K
# ! 4K5
H*! 4K5
4!5
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33333333+
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4!C-5
HF! P.##7

7

4!-/+5
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333+
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4!C"/5
#9! & 4K+/-57
H*9! & ###?7
4!";5
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3333+
<

4!C5
HF9! & #.
#
#?7
4!-5
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3333+
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4!/"5
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3.5.2. Conducting PCR:
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(

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.  7"2+Q
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8
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"

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C

   +

C

  1
3.5.3. PCR product electrophoresis:
* $.E))B
)")G
N3J#4%5B
"" )+"#"
N31)))
)

9

IV. Result and discussion
4.1 The survey field
4.1.1. Describe the symptoms in the field

8 2)
)7)#222)#
2  #  2    )#              
)2)
*)       ,        
 *)  )    49! 5 
2)
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 ) ,   
222
)
24
57 2)
22)28))2
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22)(
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10

((
((+

11

4.1.2. Surveyand white fly density of disease on the field
4.1.2.1 Tomato yellow leaf curl and white fly density developmenmt on F1 hybrid and

VL2000 in fall-winter 2010 in Phu Chan-Bac Ninh
Table 4.1.2.1 Tomato yellow leaf curl and white fly density developmenmt on F1 hybrid and
VL2000 in fall-winter 2010 in Phu Chan-Bac Ninh
No Date
Stage of
development
F1 hybrid VL2000
Prevalenc
e (%)
Whitefly
density
(individual/leaf)
Prevalenc
e
(%)
White fly density
(individual/leaf)
 "G";G" ) "2"" "2
"" "
 G";G" ) "2"" "2"
" "/
+ ;G";G" %) "2" "2"
"" "+/
 -G";G" %) 2+ "2
+" "/
 "+G"G" ) 2" "2
-" "-
- "G"G" ) 2-" "2
-" "+/
C CG"G"  -2+ "2-

C-" "/
/ G"G" 3 /2"" "2/
;" "-
; +G"G" 3 ;2+" "2C/
""" "/"
" "CGG" 3  

"2" "2
-" "-+
 !"""
 !""")

12

  )  )   )   7   ""E 
!"""-E !"""
8))""E
))""E !"""M
)))/""E;+"E4
+E52 !""";E"""E4"/E5
M)M) !"""
M)))))
)8-621
4"-G5+G"G""M"C/G
"/", !""",

,
,8$J)
4.1.2.2. Tomato yellow leaf curl and white fly density developmenmt on Hong Chau and
VL101 in fall-winter 2010 in Dong Anh – Ha Noi

Table 4.1.2.2. Tomato yellow leaf curl and white fly density developmenmt on Hong Chau and
VL101 in fall-winter 2010 in Dong Anh – Ha Noi

13

No Date
Stage of
development
Hong Chau VL101
Prevalenc
e (%)
White fly density
(individual/leaf)
Prevalenc
e (%)
White fly
density
(individual/leaf)
 "G";G"
)
"" " """ ""C
 G";G"
)
" "/ "" "-
+ ;G";G"
%)
"" "+/ "/" "
 -G";G"
%)
+" "/ -" "+

 "+G"G"
)
-" "- +-" "
- "G"G"
)
-" "+/ /" "
C CG"G"

C-" "/ -"" "/
/ G"G"
3
;" "- -/" "-
; +G"G"
3
""" "/" C-" "
" "CG"G"
3  

-" "- ;" ""
() !"
  )) !""E2
""E()()< !""/"E
$)7 !"-"E;E
;4/"E52()/"E-/"E4""E5)
C&4))57 !"-"E2();"E

14

M2) !"
1))

+

 6))7 !""/"G2()"
G

!")()
22
))
+Tomato yellow leaf curl and white fly density developmenmt on Chanoka and
and C155 in spring - summer 2011 in Vo Cuong – Bac Ninh
Table 4.1.2.3. Tomato yellow leaf curl and white fly density development onChanoka and
and C155 in spring - summer 2011 in Vo Cuong – Bac Ninh

15

No Date
Stage of
development
Chanoka C155
Prevalenc
e (%)
White fly density
(individual/leaf)
Prevalenc
e (%)
Whitefly
density
(individual/leaf)
 GG"
)

""" " "" ""C
 GG"
)
"/ " " "C
+ ;GG"
%)
 "- "; "
 -GG"
%)
+ "++ / "+
 "+GG"
)
 " ; "+;
- "GG"
)
 " +- "+"
C CGG"

- "  "/
/ GG"
3
/ "C  "+
; +GG"
3
; "- - "-"
" "CG+G"
3  

" " /" ""
)2)

B)##.
+ ,    )        
) )(2
)) 82

16

),)8)2)
7,"E/"E*)
)"/E,"E$
))8,-E/E4
/E52EE4E5
M)))28))
),"-G"-"G
4"G"G"""+G"CG"52
8)
)

             
22
!"'
4.2.PCR test results tomato samples collected in the field
M
H74(2(53!#(&
+74(5!P(!P(
74(5 !P(!P(
74(+5)PP(
-74(5*P(P(
C74&*5P&P&*
/74$5$

Table 4.2PCR test results tomato samples collected in the field

17

& < %) 9 
 ( # # #
 ( # # #
+ ( H H #
 ( H # H
 (+ H # H
- ( H H H
C &* # # #
/ $ # # #
M        )      *&#          %)
4%)G%).5,)
! 9 & /
 (2&*2$2(&F) F)
4%)G%).5G/)0
""1%)
                  ! ##  G  ! ##.  4
! 5+G/)0"
1! 
                9! & ##.  G  9! & ##
49! & 5G/))0
+/-9! & 
.$.-

 4*#(#
(54H5
  49! & 2  ! 5          )          1

))J1
?))
              )        
9! & ! 
)"""))

18


M 1 2 3 4 5 6 7 8
M 1 2 3 4 5 6 7 8
Begomovirus
19

,$.)2 9! &  ! / J
*&&&/&)&/
V. Conclusion and suggestion
5.1. Conclusion:
 )(
8)))) )2
)
   $.)! & 9 & M
 &
4  9! & 5      !      &  4   & 5   G/    
9! & 2+G/ & G/

M 1 2 3 4 5 6 7 8
ToLCVVV
TYLCVNV
20


+%)(2)$.
(M>)
,()
5.2. Suggestion:
*)22
)))7
# :)))22
 
# 
Reference
 &) 4;/52 Dieu tra nghien cuu mot so benh virus chu yeu cua ba cay
trong ho ca ( salanaceae) co y nghia kinh te ; thuoc la , ca chua, khoai tay. )!
$2#C
 J4""+52 Chan đoan nhanh benh hai thuc vat)2
(&
+ 2O)294;;52#
)* ."2;#//
 0,2(O!2(4;;C5A worldwide survey of tomato yellow leaf curl
viruses. Archives of Virology 2+;#"-

21

 0,2  (2  32  J  O  32  J  4""5       
)%#)
%)"2#+
- )(2""/2Journal of General Virology4""/52/;2+P+-5
C R,2(2!)2J2$2M4""5*&)
#)BJ%6R
"2-/#--C

/ 6). ;;/2;;;2"""2""2""2""+
; ?2J22R4""5.)
    7    S ) 1    
0 )"2#C;
" 4(2222.2$2()2.2 2JO*2R4""/5Molecular
characterization of begomoviruses and DNA satellites from Vietnam: additional
evidence that the New World geminiviruses were present in the Old World prior to
continental separationR3 )/;7+#+-5
 32:22M22!2%,2!!2.022.2J(2.2
JJ&2J299O()2!4""5 Molecular characterization of
begomoviruses associated with leafcurl diseases of tomato in Bangladesh, Laos,
Malaysia, Myanmar, and Vietnam. Plant Dis/2/-
 J,2$32%28*2!222*2.2.O%2R:
4;;5)B. tabaci4358
%7;;2-;#CB*3.J78
+ J2  B  O  &#2  R  4"""5 Tomato yellow leaf curl virus, an
emerging virus complex causing epidemics worldwide. .C2+P+
 $2  %2  *02  J#R  O  &02    4;;-5 Viral diseases causing the greatest
economic losses to the tomato crop. II. The tomato yellow leaf curl virus - a review.
Scienta Hort-C2#;-

22

 2R2%2*J2%2.M2%2R:2?2J2(2
%  *2  .,2  B  $2    )2  *    4""5  3 In "Virus
Taxonomy. VIIIth Report of the International Committee on Taxonomy of Viruses"
4J?2JJ2RJ2F*)2!%2B52
+"#+-BG$2!
- 32:4;;52 Guidelines for diagmostic works in plant virology
2&22 .*2;P;


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