Báo cáo nghiên cứu khoa học " Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs " pptx

Ministry of Agriculture & Rural Development






Progress Report

Development of an Improved Capability in
support of National Bio-security for the
Surveillance and Control of Foot & Mouth
Disease in Cattle and Pigs.


Date 30/06/2006

Table of Contents

1. Institute Information_____________________________________________ 3
2. Project Abstract ________________________________________________ 4
3. Executive Summary _____________________________________________4
4. Introduction & Background_______________________________________5
5. Progress to Date________________________________________________ 6
5.1 Implementation Highlights ____________________________________ 6
5.2 Smallholder Benefits_________________________________________ 7
5.3 Capacity Building ___________________________________________ 8
5.4 Publicity ___________________________________________________ 8

5.5 Project Management _________________________________________ 8
6. Report on Cross-Cutting Issues ___________________________________ 8
6.1 Environment _______________________________________________ 8
6.2 Gender and Social Issues_____________________________________8
7. Implementation & Sustainability Issues_____________________________ 9
7.1 Issues and Constraints_______________________________________ 9
7.2 Options____________________________________________________ 9
7.3 Sustainability_______________________________________________9
8. Next Critical Steps _____________________________________________10
9. Conclusion ___________________________________________________ 10
10. Statuatory Declaration ________________________________________ 13



1. Institute Information
Project Name

Vietnamese Institution
Regional Animal Health Centre, Ho
Chi Minh City (RAHC-HCMC),
South Vietnam.
Vietnamese Project Team Leader
Dr. Dong Manh Hoa
Australian Organisation
Australian Animal Health
Laboratory (AAHL), PMB 24,
Geelong, 3213,
Australia
Australian Personnel
Mr Chris Morrissy

Date commenced
01/06/2005
Completion date (original)
01/06/2008
Completion date (revised)

Reporting period
31/12/2005 – 30/06/2006

Contact Officer(s)
In Australia: Team Leader
Name:
Mr Chris Morrissy
Telephone:
+61 3 5227 5000
Position:
Diagnostic Virologist
Supervisor Mammalian
Virology
Fax:
+61 3 5227 5555
Organisation
Australian Animal Health
Laboratory (AAHL), PMB
24, Geelong, 3213,
Australia
Email:


In Australia: Administrative contact

Name:
Mr Chris Morrissy
Telephone:
+61 3 5227 5000
Position:
Patents Contracts Officer
Fax:
+61 3 5227 5555
Organisation
Australian Animal Health
Laboratory (AAHL), PMB
24, Geelong, 3213,
Australia
Email:

In Vietnam
Name:
Dr. Dong Manh Hoa
Telephone:
+ 84 8 8568220
Position:
Director
Fax:
+ 84 8 8569050
Organisation
Regional Animal Health
Centre, Ho Chi Minh City
(RAHC-HCMC), South
Vietnam.
Email:

2. Project Abstract
The projects purpose is to determine why there are vaccine failures, and to
investigate what serotypes of FMDV are circulating in Vietnam so that the correct
vaccines can be used for these serotypes. Regional laboratories are setup with the
reagents and methods to allow a diagnostic capability for FMDV diagnosis and
serology. Control strategies for understanding of FMD epidemiology have been
implemented through veterinary and laboratory training workshops and laboratory
testing for both virus typing and serosurveillance has begun. The project has
highlighted the importance of having a laboratory network to identify what is
happening in the field and how to prevent and control disease outbreaks. The pilot
zones are established in provinces near the borders of Vietnam to study serotypes
circulating in Vietnam and to determine their origin. The quality and collection of
samples have increased giving more data on the FMD situation in Vietnam.
Molecular epidemiological studies of the FMDV isolates in these provinces will
provide insights to the effectiveness of border control and the origin of FMDV
circulating in Vietnam each year. A diagnostic capacity for FMD would allow the early
detection and identification of disease enabling better control of disease and help reduce
loss of livestock and so productivity.

3. Executive Summary
The main focus of this reporting period was to commence the project, begin the
training of the field veterinarians in the South, Centre and North of Vietnam and also
the training of laboratory staff. All laboratory equipment was purchased and supplied
to the laboratories in Vietnam. All laboratory consumables and reagents were also
supplied to the laboratories in Vietnam for the first 6 months of the project. AAHL
staff prepared the information necessary for the training of field veterinarians. This
workshop information and the lectures were used to run workshops for the field

veterinarians in the South and Centre of Vietnam and this information was then
transferred to Dr Hoa and the staff at Ho Chi Minh City Regional Laboratory who
gave the third workshop in Hanoi. The field studies were planned and implemented
through the veterinary workshops, the field studies began with the first sero-
surveillance survey in the South and Centre of Vietnam and the collection of
outbreak samples. The Regional Animal Health Centre in Ho Chi Minh City also
visited each province to train the field veterinarians on how to collect samples and
how to collect information necessary for the history of the animals on the villages in
the project. One staff member from the Regional Animal Health laboratory and one
staff member from NAVETCO attended AAHL for training in ELISA technology for
the diagnosis of FMD and standardisation of the reagents to be used in the first year
of the project. Two AAHL staff consultants carried out workshops for the field
veterinarians in the South and Centre of Vietnam. The third veterinary workshop in
North Vietnam was carried out by DAH staff. Two AAHL consultants also carried out
a workshop for 20 participants from each of the laboratories in the project and the
regional laboratories in South Vietnam, in ELISA technologies for diagnosis of FMD
at the Regional Animal Health Centre in Ho Chi Minh City. An important part of the
laboratory training was Quality Assurance in the laboratory to ensure tests will be
run according to a standard protocol and to allow AAHL to audit the results from
each laboratory and give the appropriate data to allow trouble shooting. Avian
Influenza (AI) has extended the sero-surveillance in the first six months.


4. Introduction & Background
Objectives of the project:
1. To establish an effective laboratory network for the diagnosis and control of
FMD by the provision of resources and training of staff in required methods
and quality assurance.
2. To provide accurate data to explain failure of vaccination to control FMDV
and to develop new effective vaccine application strategies.

Completing these objectives will improve the diagnostic capability of the
Veterinary laboratories in Vietnam and the training of DAH veterinarians in
disease investigation and control. This will strengthen the profile of DAH which
will play a vital role in making Vietnam more economically competitive. Improved
animal health will lead to an increase in rural productivity though increased
animal production and indirectly in increased crop production. Healthy animals
will enable small farmers to be more competitive in the local market. Control of
FMD and animal diseases in general will give poor farmers a more stable
income stream and reduce their vulnerability to natural and economic problems.
Establishing a diagnostic network which extends from the North to South
Vietnam, from the laboratory to the farm level, reinforced by training and
education, will give Vietnam a working model on which to base disease control.
This will directly increase the competitiveness and productivity of the national
agricultural system which includes the major areas of concern including the
Mekong Delta and the Central Coast.

Implementation Approach and Strategy
The project approach is thought to be the most appropriate for developing an
understanding of FMD epidemiology in Vietnam. The approach for technology
transfer is well established at AAHL and has been successfully applied in
previous projects in Vietnam, Thailand and Indonesia. The field studies and
epidemiology and serosurveillance approaches have been designed and
planned in conjunction with DAH to provide the maximum necessary information
to demonstrate the FMD situation in Vietnam and the effectiveness of FMD
vaccines. The diagnostic technologies that will be used in this approach are the
standard diagnostic tests in use throughout the world to study FMD as directed
by OIE.
AAHL has a lot of experience with field surveys for prevalence of antibodies, as
in the ACIAR projects in Laos and Thailand on FMD. The Philippines is another

example where OIE standard diagnostic tests are being used to control and
eradicate FMD.






5. Progress to Date
5.1 Implementation Highlights
Main achievements for this reporting period were:

• Training of laboratory staff at AAHL, one representative from each laboratory
were trained in ELISA techniques for the detection of FMD antigen and
antibody and the standardisation of reagents. Training was carried out under a
quality system emphasizing the importance of Quality Assurance in the
laboratory focusing on test maintenance, record keeping and data collection.

• A consultant from AAHL completed training of HCMC-RAHC staff in cell culture
techniques and established this technology there to be used to grow FMD
isolates from the field. The growth of FMD isolates from the field is important to
allow further analysis of FMD field isolates by PCR and sequencing, Again
Quality Assurance, record keeping and data collection was emphasized.

• Further training of field veterinarians and feedback of the results from first
round of testing and shared the experience from the first round of sample
collection with the field veterinary staff to increase the quality of collected
samples.

• A consultant from AAHL visited each laboratory to complete the transfer of

ELISA technology to each laboratory and to supply the ELISA reagents.
Implementation of a Quality Assurance system into the laboratory and the
testing of sera in each laboratory was discussed. Training of staf at RAHC-
HCMC in the use of molecular techniques for the detection of FMD and the
sequencing of FMD isolates. Training of RAHC-HCMC staff in FMD serology
using cell culture to carry out the Virus Neutralisation Test (VNT)

• Sampling for second phase of sera collection completed for each province for
epidemiological studies and serosurveillance. The collection of tissue samples
from outbreaks continued to increase giving valuable information on the
serotypes circulating in Vietnam

• ELISA technology in use at collaborating laboratories in HCMC, Hanoi and Can
Tho, for serotyping of FMD tissue samples from the field. Sera from first round
of serosurveillance tested at RAHC-HCMC.

• Virus isolation in use at RAHC-HCMC. Molecular technology and serology by
VNT using cell culture has began to be used.

• Supply of consumables and further reagents for testing of samples by FMD
ELISA, molecular technology and cell culture.





Report from Vietnam:

Activities from Jan 1
st

2006 to May 30
th
2006

1. Training

No. Date & place Subject Person in charge Remark
1 02-18 Feb 06
HCM city
1. Tissue culture ( BHK 21,
BHK vacc, PK15 , BTY, Pig
kidney ,Lamb kidney, Lamb
testis )
2. Using the above cells to
isolate FMDV in Vietnam and
compare the sensitivity
between the cells
Ms. Catherine
William .
Dr. Long
Dr.Phong
Dr.Vu
4 participants
2 30 March
to 15 April 06
AAHL
1. Application of ELISA for
FMDV diagnosis (antigen and
antibody detection , Ag-ELISA
3ABC-ELISA, LP-ELISA, C-

ELISA )
2. Operation under a Quality
Assurance system
3. Reagent standardisation
Mr. Chris Morrissy
Ms. Linda
5 participants
from 4 partner
laboratories
(NVDC, RAHC-
HCMC, CAN
THO , DA
NANG ) and
Sub-DAH-
HCMC.



2. Field activities

2.1 Visit 8 provinces in the project (Middle and South Vietnam), March 2006.

- Transfer the equipment and documents (form and questionnaire) for
sample collection.

- Transfer money for sample collection.

- Feed back the results from first testing round and share the experience
on sample collection with the field veterinary staff to increase the quality
of collected samples.

- Staff in charge: Dr. Hoa, Dr. Vu , Dr. Ha ( RAHC-HCMC)
Dr. Quan, Dr.Quang (RAHC-Da Nang)
Dr. Thanh, Dr. Dung (RAHC-Can Tho)

2.1.2 Visit 2 provinces in the project (North Vietnam), March 2006.

- Transfer the equipment and documents (form and questionnaire) for
sample collection.

- Transfer money for sample collection.



- Feed back the results from first testing round and share the experience
on sample collection with the field veterinary staff to increase the quality
of collected samples.

- Staff in charge: Dr. Cam, Dr. Tung (NVDC- Hanoi)

2.1.3 Visit 3 laboratories in the Project ( NVDC-Ha Noi , RAHC-Can tho, Da nang)
early May, 2006.

- Transfer the ELISA reagents

- Discuss the plan for second testing round and share experience on
laboratory management focusing on laboratory Quality Assurance and
test management.

- Staff in charge: Mr. Chris Morrissy, Dr. Long.

2.2. Collect sera samples from 10 provinces for the second round of surveillance,
120 cattle sera and 120 pig sera from each province.

- NVDC-Ha Noi: 2 provinces (Quang Ninh and Lang Son), total 480 sera

- RAHC-Da Nang: 2 provinces (Kontum and Quang Nam), total 480 sera

- RAHC-Can Tho: 2 provinces (An Giang, Kien Giang ), total 480 sera

- RAHC-HCMC: 4 provinces (Binh Phuoc, Tay Ninh, Long An, Dong
Thap), total 960 sera

2.3 Collection of tissue samples from outbreaks for FMDV serotyping

- NVDC-Hanoi: 208 tissue samples from North Vietnam

- RAHC-Can Tho: 34 tissue samples from Mekong Delta.

- RAHC-HCMC : 390 tissue samples from the South Vietnam .

3. Laboratory Activities

3.1 National Vaterinary Diagnostic Center- Ha Noi (NVDC-Hanoi):

- Testing on tissue samples for FMDV serotyping: 208 samples, 7 of
them are serotype Asia 1 and 201 of them are Serotype O.

- Testing on sera for FMDV antibody detection: 480 sera, on going .

3.2 Regional Animal Health Centre Da Nang (RAHC-Da Nang)

- Testing on sera for FMDV antibody detection: 480 sera, on going .

3.3 Regional Animal Health Centre Can Tho (RAHC-Can Tho)



- Testing on tissue samples for FMDV serotyping : 34 samples, all of
them are serotype O.

- Testing on sera for FMDV antibody detection: 480 sera , on going .

3.4 Regional Animal Health Centre HCMC (RAHC-HCMC)

- Testing on tissue samples for FMDV serotyping: 390 samples, 1 of
them is serotype Asia 1, the remaining are serotype O.

- Testing on sera for FMDV antibody detection: 960 sera , on going.

- Isolation FMDV by BHK cell line to confirm 3 samples from NVDC-
Hanoi, 1 of them is serotype Asia1 in pigs and 1 sample is serotype O
and1 sample virus could not isolated.

- Isolation FMDV by BHK cell line from 39 selected field samples, 1
was serotype Asia1 and 38 were serotype O.

- Application of Virus Neutralisation Test (VNT) protocol (testing phase
to setup VNT technology at RAHC-HCMC):

• Virus titration for serotype O and A , two viruses for each
serotype
• VNT applied using the serum from vaccine trial on cattle

- RT-PCR application (testing phase to setup molecular technology at
RAHC-HCMC, May, 2006)

• Extract RNA from virus serotype O, A and Asia 1, two viruses
for each serotype
• Make cDNA from these RNA
• RT-PCR carried out using primers to detect serotype O and A
• Sent cDNA and amplicons to AAHL for sequencing

Reporter: Ngo Thanh Long

Note: Further detail on activities for reporting period in log frame.

5.2 Smallholder Benefits
All pig and cattle and producers are potential beneficiaries. Those that take up the
advice and use vaccine according to recommendations will benefit financially
through reduced losses due to death and disease in their cattle and pigs. The
benefits of a more profitable farming operation flow to all family members. Farmers
and district veterinarians will have improved knowledge and skill in disease
prevention, knowledge on the selection of the right vaccine and improving the
efficacy of vaccination


5.3 Capacity Building
Training and education of field veterinarians in disease prevention, disease
investigation and sample collection has been carried out in the North, Centre and

South of Vietnam. This training has already shown an impact with an increase in
quality of sample collection and number of samples collected and submitted to the
laboratory. The project has provided training and technology transfer of FMD
diagnostics to each laboratory involved in the project. Reagents and standard
methods have been supplied to each laboratory giving them the diagnostic capability
for FMDV diagnosis and serology using ELISA technology, this technology is now
able to be practised at each laboratory.
5.4 Publicity
The CARD AusAID project have received publicity through the training programs
and also through the achievements so far in the project in understanding FMD in
Vietnam. FMD is a disease on importance in Vietnam and this has put our project
into the lime light. The project has been publicised through a press release in
Australia and articles in news letters including the SEAFMD newsletter.
5.5 Project Management
Implementation of the project is on schedule. AAHL has kept the project on target
and has supported or requests from the Vietnamese partners. Training has been
carried out in Australia and Vietnam with both partners benefiting from the
information that is being generated.
Implementation in Vietnam is also continuing to go well. Trainees have been
supplied in a timely manner, from DAH. The field work has been organised well with
data and samples being collected from farms in each pilot zone. Training and the
transfer of information to other regional laboratories in Vietnam is also occurring.
RAHC-HCMC will supply support to these laboratories.
6. Report on Cross-Cutting Issues
6.1 Environment
No direct environmental impacts associated with project activities have been
identified. On a broader scale, the intention is that pig and cattle farming will become
more efficient through a reduction in animal death and disease. Environmental
benefit will result through more efficient utilization of resources such as animal feed,
the energy to provide animal feed and to run other aspects of the farm.

6.2 Gender and Social Issues
Application of new diagnostic tests will improve the ability of regional and provincial
diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and
appropriate measures to be applied to control disease. These benefits will begin
during the project and continue to accrue with continued application. The
beneficiaries of this project will be both large and small farms and particularly
smallholder farmers whose animals and incomes will be protected by better disease
diagnosis, management and control. Since women at the village level are the
primary animal handlers and managers, they will be major beneficiaries of the final
outcome of better diagnosis and control of animal diseases.


7. Implementation & Sustainability Issues
7.1 Issues and Constraints
DAH has been very busy controlling the AI outbreaks in Vietnam and this has made
there workload increase dramatically, this have meant some delays and
rescheduling of training field veterinarians, eg training in the North Vietnam
rescheduled. Sampling has also been spread out over a longer time period as well
but this has had no effect with all samples still being collected. The collection of
samples was made more difficult by the need to train all veterinarians in each
province in data collection, on how to collect samples, collection of blood and how to
restrain cattle. This increased training need has highlighted the need for further
training of field veterinarians throughout Vietnam in disease investigation techniques
with a focus on sample collection and data collection. There is a need for further
input in this area, large scale training of field veterinarians to ensure better
knowledge and control of disease in Vietnam. This could be achieved though better
collaboration of aid agencies in Vietnam especially those supplying training for AI.
7.2 Options
The government of Vietnam is looking at increasing the support to DAH and has
increased funding for AI diagnosis and is looking to do the same for FMD diagnosis.

The increased funding for AI has seen laboratories updated with new equipment
some of which will improve all disease diagnosis which includes FMD.
The money available to improve AI diagnosis can improve all disease diagnosis if
this money is used wisely, eg in training field veterinarians in AI diagnosis, the
training should be general to cover all diseases including FMD, CSF and other
diseases of importance in Vietnam. Also in improving AI diagnosis in laboratories the
training should emphasize how improvement can be applied to all areas of
diagnosis, eg quality assurance in the laboratory.

7.3 Sustainability
The DAH laboratories appear to be well supported by the central government. The
DAH laboratories are also receiving increased support from overseas funding
agencies as part of the AI campaign to improve laboratory facilities.
RAHC-HCMC is developing in confidence as an institution. This confidence is
beginning to be passed on to other DAH laboratories. The DAH scientific staff are
quick to understand new information and are energetic in its implementation. People
have an obvious desire to do their jobs well. This desire is obvious in staff in RAHC-
HCMC and other laboratories. The laboratories just need technical support to be
encouraged to carry out the techniques that they need for diagnosis and research.
The project is following a well tried model for successful technology transfer, of
demonstration and teaching of the technology in the donor facility followed by supply
of reagents for implementation by the trainees at their own initiative in the recipient
laboratory, subsequently backed up by consultancy visits to the recipient laboratory
for support and fine tuning. A useful indicator of likely success is the initiative and
ability of the trainees to make a useful attempt at implementation by themselves.
DAH and NAVETCO staff show a high level of involvement in this way.
The AusAID funded CSFV project, that linked DAH, NaVetCo and AAHL prior to this
project, is proving sustainable. NAVETCO and DAH not only maintains the
diagnostic capability developed at that time, but on its own initiative and unassisted

by AAHL is conducting training courses and technology transfer to other labs in
Vietnam.
8. Next Critical Steps
In the next 6 months a meeting will be held at AAHL to review the project outputs
and to examine the information collected so far in the project, The meeting will
decide if the data collected from the field is giving DAH the necessary information to
make discisions on FMD control. An AAHL empidemilogist will be involved in
collating the data from the first 2 phases od serosurveillance and epipdemlogical
data collection and begin exposing DAH staff to data analysis techniques. The third
phase of the sero-surveillance for the collection of the samples will be implemented
with any changes necessary. In the next 6 months we will finalise all of the testing of
the samples collected and evaluate each laboratories capabilities. In the second 6
months of the project it was decided to bring forward the training of the staff from
each laboratory to be held at AAHL. This training will be carried out at AAHL for 1
representative from each laboratory for training in ELISA techniques.
A consultant from AAHL will complete training of the HCMC-RAHC in cell culture
techniques and establish this technology there to be used to grow FMD isolates from
the field. The growth of FMD isolates from the field is important to allow further
analysis of these isolates by PCR and sequencing.
9. Conclusion
The project activities are on schedule. The DAH laboratories are running the field
trials on time, even though they have had increased workload with avian influenza
outbreaks. It is expected that the second sero-surveillance survey will be much
easier than the first. The first took a lot longer to do due to commitments with avian
influenza and also understanding the problems associated with collecting samples in
areas where samples had not been collected before. Field veterinarians needed to
be trained in the collection of tissue samples from infected animals as well as
bleeding animals. The staff at Regional Animal Health Centre, Ho Chi Min City have
performed a excellent job in getting everything to run on schedule in this first six

months. The project should continue to run on time and I expect no further problems
especially how the collection procedures are in place. The staff at DAH are very
eager to learn and this is making a very good environment for transferring
technology. The laboratory at Da Nang has less experience and this laboratory will
need much more contact with AAHL and the laboratory at RAHC-HCMC to achieve
the transfer of the ELISA technology to this laboratory.
The laboratory in Hanoi has the skills and is doing quite well in the application of the
ELISA but this laboratory needs further contact with AAHL to increase their
understanding of the application of this technology for FMD diagnosis and this also
applies to the laboratory in Can Tho in the south. Can Tho is applying the
technologies learnt.
This project has proved to be an important project for Vietnam with the importance of
FMD and this has meant good publicity for the AusAID CARD program. It has also
meant an increase in requests for help in other areas of animal health with the
success of this project in improving the skills and technology at RAHC-HCMC and
NAVETCO and through our other project in CSF at NAVETCO and DAH and Duck
Plague at NAVETCO. DAH is using this project as an example of how to approach
FMD control.



10. Statutory Declaration
STATUTORY DECLARATION

COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT
PROGRAM

CARD Project Title: FMD - Improved surveillance capabilities for FMDV
CARD Project Number: 072/04VIE

We the undersigned hereby declare that during the period 01/06/2005 to 31/12/2005
we have delivered the following inputs to assist in implementation of the above
project.

1. PERSONNEL INPUTS

Australian Personnel
Provided (Name)
Days in
Vietnam
Days in
Australia
Trips to
Vietnam
Chris Morrissy (also
replaced Laurie Gleeson’s
time in project)
28 10 2
Peter Daniels 14 1
Lynda Wright (replaced
Brenda Van Der Heide in
project )
14 1
Greer Meehan 10
Axel Colling 5
Catherine Williams 15
Total 56 40 4


Vietnamese Personnel

Provided

Days in Vietnam
Dr.Bui Quang Anh 10
Dr.Hoang Van Nam 20
Dr.Tran Ngoc Thang 15
RAHC-HCMC
Dr.Dong Manh Hoa 25
Ngo Thanh Long, DVM 25
Nguyen Truc Ha, Ms 20
Pham Phong Vu, DVM 25
Nguyen Ngo M. Triet, DVM 25
NVDC-HA NOI



Dr. To Long Thanh 15
Dr. Nguyen Thu Ha 20
Nguyen Tung, Ms 20
RAHC-DA NANG
Tran Van Quan,DVM 15
Le Thanh Quang, DVM 20
Huynh Thi Manh, Ms 20
RAHC-CAN THO
Nguyen Ba Thanh, Ms 15
Truong Kim Dung, Ms 20
Tran Quoc Phong, DVM 20
NAVETCO
Dr.Tran Xuan Hanh 15
Nguyen Thu Hong, Ms 20

Nguyen Van Hung, Bc 20



























2. EQUIPMENT AND OTHER SERVICES

Equipment & Other Services
Description
Budget Limit
Inverted Microscope 4000
Pipettes 5000
Microfuge 3000
Laptop 4000
Reagents
4000
Sample Collection
8755
Molecular technologies
2000
Laboratory consumables
4000
Freight
14000
ELIZA work
5000


Project Progress Against Proposed Objectives, Outputs, Activities And Inputs
Project Title:
Vietnamese Implementing Institution:
PROPOSAL PROGRESS REPORT
Narrative Information Required Performance
Measures
Assumptions Information Required

OBJECTIVES
1.






To establish an effective
laboratory network for the
diagnosis and control of FMD by
the provision of resources and
training of staff in required
methods and quality assurance.
(Objective 1.2.2, 2.1.1 & Strategy
1)


Samples collected and
submitted to laboratory.
Data produced and results
feedback to field veterinary
staff and farms.

Technology will be
taken up by
laboratories.
Accurate data
collected from farms.
Little risk.

Establishment of an effective laboratory
network for the diagnosis and control of FMD
continued with training given at AAHL under a
Quality system, method established at each
laboratory, QA introduced and resource
supplied. The four DAH laboratories are
working together to carry out surveillance and
testing.

1.1

Train field veterinary staff in
outbreak investigation for the
investigation of cases of FMD on
farms, including record keeping,
sampling strategies and data
collection.



Samples collected from
field and submitted to
laboratory. Sample and
farm records presented with
samples.

Accurate data
collected from farms.

Little risk

Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.

1.2

Establish ELISA technology from
AAHL to collaborating
laboratories
Diagnostics test in use. QA
controls meet. internal
quality control sheets for
examination, correct results
in external proficiency
testing organized by AAHL.
Results generated.


Technology will be
taken up by
laboratories. Little
risk

ELISA training and reagents delivered.
Laboratories have began to use the ELISA
technology to test samples.

Narrative Information Required Performance Measures Assumptions Information Required

1.3


Using cell culture at RAHC-
HCMC, make a collection of
isolates of FMD viruses from
cases of FMD disease on farms.


Virus isolates grown in cell
culture

Cell cultures can be
maintained at RAHC-
HCMC. Little risk.


Training at AAHL carried out and cells
transferred to RAHC-HCMC. FMD samples
collected. Further training in HCMC this 6
months and cell culture established at RAHC-
HCMC

1.4

At RAHC-HCMC and AAHL use
molecular techniques to assess

strains of FMD circulating




Sequence data produced.

Technology will be
taken up by
laboratories. Little
risk

FMD samples collected

1.5

Introduce cell culture to NVDC-
Hanoi for FMD diagnosis




Cell culture in use at
NVDC-Hanoi

Technology will be
taken up by
laboratories. Little
risk

Activity later in project

1.6

Firm link with AAHL for the
ongoing supply of diagnostic
reagents.

Diagnostic reagents used
for routine diagnostics.

Reagents available.
Little risk.




Process began

2.

To provide accurate data to
explain failure of vaccination to
control FMDV and to develop new
effective vaccine application
strategies. (Objective 1.2.2, 2.1.1
& Strategy 1)


Project Report presented to

DAH and AusAID.
Feedback meetings held in
provinces.

Project objectives
meet. Little risk.

FMD samples collected




Narrative Information Required Performance Measures Assumptions Information Required

2.1






2.2





2.3

2.4


2.5





Hold information and training
workshops for farmers and their
veterinary advisors, with
emphasis on FMD prevention
and disease investigation and
sample collection

Pilot zones established in each
province.




Feedback to collaborating
laboratories results from test
comparisons at AAHL and
RAHC-HCMC.

Feedback results to veterinary

field staff and farmers.

Determine the efficacy of the
FMD vaccine in protecting
against FMD isolates circulating
in Vietnam.


Samples collected from
field and submitted to
laboratory. Sample and
farm records presented with
samples.


Samples collected from
field and submitted to
laboratory. Sample and
farm records presented with
samples.

Results on laboratory
comparisons and EQA
send back to each
laboratory.

Results maintained at field
veterinary office

Comparison of FMD

genotypes to vaccine.
Results of serosurveillance.



Accurate data
collected from farms.
Little risk




DAH is able to have
farmers agree to
project. Little risk



Technology will be
taken up by
laboratories. Little
risk

Samples collected
and tested. Little risk.

Samples collected
and tested. Little risk.

Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff. Further information to farmers
to be delivered later in project



Pilot zones established. First round of
serosurveillance completed.




Activity later in project




Activity later in project


Activity later in project. Samples collected.

2.6




Hold information and training
workshops farmers and their
veterinary advisors, with
emphasis on FMD prevention
and presenting the data
obtained during the CARD
project.

Meetings held with farmers
and their veterinary
advisors

Project will achieve
objectives.
Little risk.

Samples collected


OUTPUTS
1. & 2.

• Field veterinarians that are
skilled in on farm diagnosis
and sample collection.
• Education of farmers to allow
better understanding of the
benefits of diagnostic
technologies.

• Development of cell culture
capability for virus
propagation, isolation from
field samples and for serology
by virus neutralisation test
(VNT).
• Diagnostic capabilities at all
the laboratories participating
in the project to carry out
functional tests as described
under a Quality System.
• Pilot zones established and
operational.
• Firm link with AAHL for the
ongoing supply of diagnostic
reagents.
• Application of technologies,
knowledge and skills to other

Samples collected and
submitted to laboratory.
Data produced and results
feedback to field veterinary
staff and farms.

Technology will be
taken up by
laboratories.
Accurate data
collected from farms.
Little risk.

Process began. Training carried out in the
North, Centre and South of Vietnam for
veterinary field and laboratory staff.
Activity later in project




Training at AAHL carried out and cells
transferred to RAHC-HCMC. FMD samples
collected

Establishment of an effective laboratory
network for the diagnosis and control of FMD
began with training given, method
established, QA introduced and resource
supplied.

Pilot zones established. First round of
serosurveillance completed.

Process began.

Activity later in project

1.1-1.6 &
2.1- 2.6







diseases.
• Increased productivity by
small farm holders with more
food available to poor
farmers. This will have a
more pronounced effect on
the women and children who
are mainly responsible for the
animals’ upkeep and likely to
obtain the major benefits of
increased crop production
and sale of animals.

• Field veterinarians trained in
on farm diagnosis and
sample collection.


• Education of farmers to allow
better understanding of the
benefits of diagnostic

technologies to disease
control.
• Setup farms/villages in each
province for seroservalance




• Transfer of ELISA technology
to collaborating laboratories


















Samples collected from
field and submitted to

laboratory. Sample and
farm records presented with
samples.

Farmers participating in
projects


Samples collected from
field and submitted to
laboratory. Sample and
farm records presented with
samples.

Diagnostics test in use. QA
controls met, internal
quality control sheets
available for examination,
correct results in external

Accurate data
collected from farms.
Little risk



DAH is able to have
farmers agree to
project. Little risk

DAH is able to have
farmers agree to
project. Little risk



Technology will be
taken up by
laboratories. Little
risk


Expected outcome at end of project

Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.



Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.

Pilot zones established. First round of
serosurveillance completed.

Establishment of an effective laboratory
network for the diagnosis and control of FMD
began with training given, method
established, QA introduced and resource






• ELISA established in each
laboratory and EQA carried
out in each laboratory


• Transfer cell culture capability
to RAHC-HCMC


• Cell culture used to grow
FMDV from field isolates


• Transfer molecular
techniques for FMDV RT-
PCR to RAHC-HCMC

• Train RAHC-HCMC staff in
sequence analysis of
sequenced FMD isolates

• Train NVDC in use of cell
culture for FMD diagnosis

• Communication of diagnostic
results to field veterinarians
and farmers

• Recommendations on
efficacy of FMD vaccines

proficiency testing
organized by AAHL.
Results generated.

Results on laboratory
comparisons and EQA
send back to each
laboratory.

Virus isolates grown in cell
culture.


FMDV isolates sequenced



PCR products send to
AAHL

Sequence data produced.



Trainee at AAHL from
NVDC-Hanoi


Results maintained at field
veterinary offices


Comparison of FMD
genotypes to vaccine.





Technology will be
taken up by
laboratories. Little
risk

Cell cultures can be
maintained at RAHC-
HCMC. Little risk.

Cell cultures can be
maintained at RAHC-
HCMC. Little risk.

Technology will be
taken up by
laboratories. Little
risk

PCR products
submitted to AAHL


Trainee does not
attend training. Little
risk

Samples collected
and tested. Little risk.


Samples collected
and tested. Little risk.
supplied.





Process began.

Training at AAHL carried out and cells
transferred to RAHC-HCMC.


FMD samples collected




Activity later in project




Activity later in project



Activity later in project



Activity later in project

Activity later in project


• Comparison of FMD isolates
circulating in Vietnam

• Reagents in use in DAH
laboratories and purchase of
reagents by DAH for ongoing
epidemiology studies and
serosurveillance.

• Final project report written
summarising results obtained
from project

• Report to DAH and results of
project to be published
Results of serosurveillance.
Report on sequence
analysis of FMD isolates

Diagnostic reagents used
for routine diagnostics.



Meetings held with farmers
and their veterinary
advisors.

Final reports written.

FMD Isolates
collected. Little risk.

Reagents available.
Little risk



Project will achieve
objectives. Little risk.


Project will achieve
objectives. Little risk.



Activity later in project


Activity later in project




Activity later in project

Activity later in project

ACTIVITIES
1. & 2.



















To establish an effective
laboratory network for the
diagnosis and control of FMD by
the provision of resources and
training of staff in required

methods and quality assurance.

To provide accurate data to
explain failure of vaccination to
control FMDV and to develop new
effective vaccine application
strategies.



.




Major activity/objective of
the project, to be
completed over the 3
years of the project

Diagnostic
capabilities at all the
laboratories
participating in the
project to carry out
functional tests as
described under a
Quality System.
Field veterinarians
that are skilled in on
farm diagnosis and
sample collection.
Education of farmers
to allow better
understanding of the
benefits of diagnostic
technologies.

Pilot zones

Establishment of an effective laboratory
network for the diagnosis and control of FMD
began with training given, method
established, QA introduced and resource
supplied.

Pilot zones established. First round of
serosurveillance completed.






















• Order and purchase
equipment

• Supply laboratory

consumables

• AAHL staff will prepare
information and lectures for
workshop for training and
education of field
veterinarians and farmers

• Run first workshop at RAHC-
HCMC field veterinarians

• Supply consumables and
budget for sample collection

• RAHC-HCMC to initiate
epidemiological and
serosurveillance programs


• RAHC-HCMC and NCDC-
Hanoi to coordinate training
and meetings with field

First month year 1


First 3 months year 1


First 3 months year 1





First 3 months year 1


First 3 months year 1


First 3 months year 1





First 3-6 months year 1


established and
operational.

Recommendations
on vaccine
application from
epidemiological and
serosurveillance
data.



Equipment in place at
laboratories

Consumables at
laboratories
Workshop held at
RAHC-HCMC


Workshop held at
RAHC-HCMC

Consumables and
budget supplied

Contact made with
field veterianians to
begin sample
collection for disease
outbreaks

Workshop &
meetings held









Equipment in place at laboratories


Consumables supplied to laboratories


Completed. Information supplied and used in
workshops




Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.
Consumables and budget supplied

Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.



veterinarians in provinces
where fields studies are to be
carried out.

• Hold meetings to educate
farmers on the benefits of
epidemiological studies,
serosurveillance and
laboratory diagnostics to
disease control

• Sampling schedule setup for
each province in the project



• Farms/village setup with
information on sampling
schedule and project

requirement


• Costs of sampling negotiated
with farmers and field
veterinary staff

• Serosurveillance carried in
each province


• Samples collected from field
and tested in each laboratory

• Scientists from RAHC-HCMC
laboratories to be trained in




First 3-6 months year 1






First month year 1

First 3 months year 1





First 3 months year 1



Twice a year for 3 years



Twice a year for 3 years



First 3 months year 1




Meetings held

Document produced
for sampling
schedule


Document for project
field studies
circulated to filed
veterinarians and
farmers

Payment details
completed


Samples collected
and send to
laboratories

Results generated



Scientists trained at
AAHL

Initial contact made with farmers in project.
Education to be carried out as part of National
program




Sampling schedule documented during
workshop for Veterinarians. Pilot zones
established. First round of serosurveillance
completed.

Pilot zones established. First round of
serosurveillance completed.




Budget for field work received and transfer to
field staff.


Completed



FMD outbreak samples tested by ELISA.
Activity to continue later in project

Completed




FMD diagnostics, ELISAs and
cell culture at AAHL.

• Follow up training by AAHL to
establish ELISA and cell
culture techniques at RAHC-
HCMC.

• Cell cultures and
consumables supplied to
RAHC-HCMC

• Cell cultures grown and
stored at RAHC-HCMC

• Cell cultures used for virus
isolation and serology


• AAHL to run ELISA workshop
at RAHC-HCMC

• Follow training by RAHC-
HCMC staff to each

laboratory

• FMD ELISAs established in
regional laboratories.

• Samples send from each
laboratory to RAHC-HCMC
and AAHL for comparison

• Accreditation of laboratory for
FMD ELISAs



First 3 - 6 months year 1




First 3 - 6 months year 1



First 3 - 6 months year 1


7-8th month of year 1

First 6 months year 1



First 3 - 6 months year 1


First 6-9 months year 1




Twice a year for 3 years


First 6-9 months year 1



Visit by AAHL
consultant to RAHC-
HCMC


Cell cultures &
consumables
delivered

Cell cultures in use

FMDV isolated from
field SNT used for
serology

Workshop completed






ELISA working in
each laboratory



ELISA results send to
AAHL

Results of
comparisons reported
back to laboratories


Activity later in project

Completed



Completed


Activity later in project



Completed



Completed


Activity later in project




Activity later in project


Activity later in project