Báo cáo nghiên cứu khoa học " Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs - Milestone 5 " ppt

CSIRO-AAHL AUSAID DAH-VN

CARD-072/04VIE



Ministry of Agriculture & Rural Development






062/05VIE

Development of an Improved Capability in support
of National Bio-security for the Surveillance and
Control of Foot & Mouth Disease in Cattle and Pigs.

Milestone 5
: Progress Report



Date 31/12/2006

1
Table of Contents

1. Institute Information___________________________________________________ 3
2. Project Abstract _______________________________________________________ 4

3. Executive Summary ____________________________________________________ 4
4. Introduction & Background _____________________________________________ 5
5. Progress to Date _______________________________________________________ 6
5.1 Implementation Highlights _________________________________________ 6
5.2 Smallholder Benefits_______________________________________________ 7
5.3 Capacity Building _________________________________________________ 8
5.4 Publicity_________________________________________________________ 8
5.5 Project Management_______________________________________________ 8
6. Report on Cross-Cutting Issues___________________________________________ 8
6.1 Environment _____________________________________________________ 8
6.2 Gender and Social Issues ___________________________________________ 8
7. Implementation & Sustainability Issues ____________________________________ 9
7.1 Issues and Constraints _____________________________________________ 9
7.2 Options__________________________________________________________ 9
7.3 Sustainability_____________________________________________________ 9
8. Next Critical Steps ____________________________________________________ 10
9. Conclusion __________________________________________________________ 10
10. Statuatory Declaration_______________________________________________ 12

3
1. Institute Information
Project Name

Vietnamese Institution
Regional Animal Health Centre, Ho
Chi Minh City (RAHC-HCMC), South
Vietnam.
Vietnamese Project Team Leader
Dr. Dong Manh Hoa
Australian Organisation

Australian Animal Health Laboratory
(AAHL), PMB 24, Geelong, 3213,
Australia
Australian Personnel
Mr Chris Morrissy
Date commenced
01/06/2005
Completion date (original)
01/06/2008
Completion date (revised)

Reporting period
007611201/07/2006 – 31/12/2006

Contact Officer(s)
In Australia: Team Leader
Name:
Mr Chris Morrissy
Telephone:
+61 3 5227 5000
Position:
Diagnostic Virologist
Supervisor Mammalian Virology
Fax:
+61 3 5227 5555
Organisation
Australian Animal Health
Laboratory (AAHL), PMB 24,
Geelong, 3213,
Australia

Email:


In Australia: Administrative contact
Name:
Mr Chris Morrissy
Telephone:
+61 3 5227 5000
Position:
Patents Contracts Officer
Fax:
+61 3 5227 5555
Organisation
Australian Animal Health
Laboratory (AAHL), PMB 24,
Geelong, 3213,
Australia
Email:

In Vietnam
Name:
Dr. Dong Manh Hoa
Telephone:
+ 84 8 8568220
Position:
Director
Fax:
+ 84 8 8569050
Organisation
Regional Animal Health Centre,

Ho Chi Minh City (RAHC-
HCMC), South Vietnam.
Email:


4
2. Project Abstract
The projects purpose is to determine why there are vaccine failures, and to investigate what
serotypes of FMDV are circulating in Vietnam so that the correct vaccines can be used for
these serotypes. Regional laboratories are setup with the reagents and methods to allow a
diagnostic capability for FMDV diagnosis and serology. Control strategies for understanding
of FMD epidemiology have been implemented through veterinary and laboratory training
workshops and laboratory testing for both virus typing and sero-surveillance have begun. The
project has highlighted the importance of having a laboratory network to identify what is
happening in the field and how to prevent and control disease outbreaks. The pilot zones are
established in provinces near the borders of Vietnam to study serotypes circulating in
Vietnam and to determine their origin. The quality and collection of samples have increased
giving more data on the FMD situation in Vietnam. Molecular epidemiological studies of the
FMDV isolates in these provinces will provide insights to the effectiveness of border control
and the origin of FMDV circulating in Vietnam each year. Virus isolation and molecular
studies have started to be carried out on FMD samples from the field. A diagnostic capacity
for FMD would allow the early detection and identification of disease enabling better control of
disease and help reduce loss of livestock and so productivity.

3. Executive Summary
The main focus of this reporting period was to establish FMD diagnostics in the collaborating
laboratories with a focus on the HCMC laboratory. All consumables and reagents were
supplied to the laboratories in Vietnam for the second year of the project. AAHL staff
prepared the protocols necessary for the training of laboratory scientists. Two scientists from
the Regional Animal Health laboratory HCMC trained at AAHL in molecular techniques for

the diagnosis of FMD and standardisation of the protocols and reagents to be used in the
project for sequencing FMD isolates from Vietnam.
The project carried out training at the HCMC and Hanoi laboratories in molecular
technologies for diagnosis of FMD and reviewed ELISA results for the first round of sero-
serveillance. An important part of the laboratory training was Quality Assurance (QA) in the
laboratory to ensure tests were running according to a standard protocol and with Internal
Quality Assurance (IQC) to allow AAHL staff to audit the results from the laboratory and
give the appropriate data to allow trouble shooting. The QA records were reviewed during
training visit to the laboratories.
The Regional Animal Health Centre in Ho Chi Minh City also continued to train the field
veterinarians on how to collect samples and how to collect information necessary for the
history of the animals on the villages in the project. It was identified that data collected from
the field was not suitable from the first round of sero-surveillance. The funding was supplied
for the second year of field studies.
Avian Influenza (AI) continues to delay the sero-surveillance in the field as field staff deal
with the AI outbreak. .

5
4. Introduction & Background
Objectives of the project:
1. To establish an effective laboratory network for the diagnosis and control of FMD
by the provision of resources and training of staff in required methods and quality
assurance.
2. To provide accurate data to explain failure of vaccination to control FMDV and to
develop new effective vaccine application strategies.

Completing these objectives will improve the diagnostic capability of the Veterinary
laboratories in Vietnam and the training of DAH veterinarians in disease investigation
and control. This will strengthen the profile of DAH which will play a vital role in
making Vietnam more economically competitive. Improved animal health will lead to

an increase in rural productivity though increased animal production and indirectly in
increased crop production. Healthy animals will enable small farmers to be more
competitive in the local market. Control of FMD and animal diseases in general will
give poor farmers a more stable income stream and reduce their vulnerability to natural
and economic problems. Establishing a diagnostic network which extends from the
North to South Vietnam, from the laboratory to the farm level, reinforced by training
and education, will give Vietnam a working model on which to base disease control.
This will directly increase the competitiveness and productivity of the national
agricultural system which includes the major areas of concern including the Mekong
Delta and the Central Coast.

Implementation Approach and Strategy
The project approach is thought to be the most appropriate for developing an
understanding of FMD epidemiology in Vietnam. The approach for technology transfer
is well established at AAHL and has been successfully applied in previous projects in
Vietnam, Thailand and Indonesia. The field studies and epidemiology and sero-
surveillance approaches have been designed and planned in conjunction with DAH to
provide the maximum necessary information to demonstrate the FMD situation in
Vietnam and the effectiveness of FMD vaccines. The diagnostic technologies that will be
used in this approach are the standard diagnostic tests in use throughout the world to
study FMD as directed by OIE.
AAHL has a lot of experience with field surveys for prevalence of antibodies, as in the
ACIAR projects in Laos and Thailand on FMD. The Philippines is another example
where OIE standard diagnostic tests are being used to control and eradicate FMD.




5. Progress to Date
5.1 Implementation Highlights

Main achievements for this reporting period were:


6
• Training of laboratory staff at AAHL. Two representatives from the HCMC laboratory
were trained in molecular techniques for the detection of FMD genome and the
standardisation of reagents and protocols needed to establish PCR in HCMC. Training
was carried out under a quality system emphasizing the importance of Quality
Assurance in the laboratory focusing on test maintenance, record keeping and data
collection.

• A consultant from AAHL completed training of HCMC-RAHC staff in molecular
techniques and established the technology to be used to identify FMD isolates from the
field in the HCMC laboratory. Again Quality Assurance, record keeping and data
collection was emphasized.

• AAHL consultant reviewed cell culture and virus isolation for growth of FMD isolates
from the field. Cell culture is important to grow FMD virus to allow further analysis of
FMD field isolates by PCR and sequencing,

• Further training of field veterinarians was carried out with feedback of the results from
first rounds of testing and experiences from the first round of sample collection to
increase the quality of samples and data collected.

• The consultant from AAHL also reviewed the transfer of ELISA technology to each
laboratory and the implementation of a Quality Assurance system into the laboratory.
The testing of sera in each laboratory was discussed with the Nth Vietnam sample
collection needing to improve. Also training of RAHC-HCMC staff in FMD serology
using cell culture to carry out the Virus Neutralisation Test (VNT) was part of training.

• Sampling for next round of sera collection completed for each province for
epidemiological studies and sero-surveillance. The collection of tissue samples from
outbreaks continued to increase giving valuable information on the serotypes
circulating in Vietnam

• ELISA technology in use at collaborating laboratories in HCMC, Hanoi and Can Tho,
for sero-typing of FMD tissue samples from the field. Sera from sero-surveillance
tested at RAHC-HCMC and Hanoi laboratories.

• Virus isolation in use at RAHC-HCMC. Molecular technology and serology by VNT
using cell culture has started to be used.

• Supply of consumables and further reagents for testing of samples by FMD ELISA,
molecular technology and cell culture.

• Project meeting held at AAHL with Director of DAH, Vietnam and Director of
RAHO-HCMC to discuss project outcomes. Training in QA and biosafety given. Also
held a meeting in Canberra with DAFF to discuss Australia’s preparedness for disease
outbreaks. Project achieving aims for the DAH in establishing framework for control of
FMD.


Report from Vietnam:

30/06/31/12/ACTIVITIES from June 1
st
2006 to Dec 31
th
2006

7
1.Training
No. Date & place Subject Person in
charge
Remark
1 July 2006






November
2006

-Expert from AAHL
working in Viet Nam to
review data from first round
of sero-surveillance.
Discuss improved data
collection and records

Expert from AAHL to
complete molecular training
and review FMD
diagnostics in collaborating
laboratories
Chris Morrissy
and Peter Durr

Chris Morrissy
Visit HCMC and
Hanoi laboratories
2 23
th
. Oct. -
8
th
.Dec., 2006
AAHL,
Australia
-Sequencing and analysis of
FMDV
-Virus Inactivation by BEI
to produce antigen for
ELISA , practice on Blue
Tongue virus and VSV

- Chris
Morrissy
- Ian Pritchard
- Tony Pye
- Kim Newbery
- Lynda Wright
- Cathy
Williams

- 1 Participants from
RAHC-HCMC

3 -13
th
.
Nov. – 8
th
.
Dec., 2006
- Analysis of FMDV
sequencing data.
-Virus Inactivation by BEI
to produce antigen for
ELISA , practice on Blue
Tongue virus and VSV

- Chris
Morrissy
- Ian Pritchard
- Tony Pye
- Kim Newbery
- Lynda Wright
- Cathy
Williams

- 1 participant from
RAHC-HCMC
4 13

th
.
-18
th
.
Nov, 2006
In Australia
-Review the project

- Chris
Morrissy
- Dr.Bui Quang Anh
(Director General of
DAH of Viet Nam)
- Dr.Dong Manh
Hoa ( Director of
RAHC-HCMC,
main Viet Nam
partner)

2. Field activities

2.1.1. Visiting 8 provinces in the project (Middle and Southern ) , Oct. 2006.
• Transfer the equipments and documents ( form, questionnaire) for sample collection.
• Transfer money for sample collection.
• Feed back the results from second testing round and share the experience
on sample collection with the field veterinarian staff to increase the quality of
collected samples .
• Staff in charge : Dr. Hoa, Dr. Vu , Dr. Ha (RAHC-HCMC) - Dr. Quan , Dr.Quang
(RAHC-Da Nang) – Dr. Thanh , Dr. Dung (RAHC-Can Tho)

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2.1.2. Visiting 2 provinces in the project ( North ) , Oct. 2006.
• Transfer the equipments and documents (form, questionnaire) for sample
collection.
• Transfer money for sample collection.
• Feed back the results from second testing round and share the experience
on sample collection with the field veterinarian staff to increase the quality of
collected samples .
• Staff in charge : Dr. Cam , Dr. Tung ( NVDC- Ha Noi)

2.2. Collect sera samples from 10 provinces for the third round of surveillance , 120 cattle
sera and 120 pig sera from each province.

2.2.1 NVDC-Ha Noi : 02 provinces (Quang Ninh and Lang Son) , total 480 sera samples.
2.2.2 RAHC-Da Nang : 02 provinces (Kontum and Quang Nam) , total 480 sera samples.
2.2.3 RAHC-Can Tho : 02 provinces (An Giang, Kien Giang ), total 480 sera.
2.2.4 RAHC-HCMC : 04 provinces (Binh Phuoc, Tay Ninh, Long An, Dong Thap), total
960 sera

2.3 Collect tissue samples for FMDV typing
2.3.1 NVDC-Ha Noi : 103 tissue samples from the North ,
2.3.2 RAHC-HCMC : 189 tissue samples from the South .

3. Laboratory Activities

3.1 National Vaterinary Diagnostic Centre- Ha Noi ( NVDC-Ha Noi):
3.1.1 Testing on tissue samples for FMDV typing: 103 samples, all of them are serotype O
3.1.2 Testing on sera for FMDV antibodies detection: 480 sera , test finished

3.2 Regional Animal Health Center Da Nang (RAHC-Da Nang)
3.2.1 Testing on sera for FMDV antibodies detection : 480 sera, test finished
3.2.2 Sending 480 sera to RAHC-HCMC for retest : 480 sera

3.3 Regional Animal Health Center Can Tho (RAHC-Can Tho)
3.3.1 Testing on sera for FMDV antibodies detection : 480 sera, test finished
3.2.2 Sending 480 sera to RAHC-HCMC for retest: 480 sera

3.4 Regional Animal Health Center HCMC(RAHC-HCMC)
3.4.1 Testing on tissue samples for FMDV typing: 189 samples, 01 of them is serotype
Asia 1, 179 samples are serotype O and 09 samples are negative
Remark: test covers 3 serotype: serotype A, O and Asia 1
3.4.2 Testing on sera for FMDV antibodies detection: 1920 sera ( 960 sera in the region
and retest 960 sera from RAHC-Can Tho and Da Nang ). Test finished

3.4.3 Isolate FMDV by BHK cell line from 32 selected samples, 01 serotype Asia1 and 31
serotype O.
3.4.4 Application of SNT protocol in testing phase :
Adapt 5 FMDV on BHK cell line to produce reference virus for using in SNT for
• Serotype O, PanAsia ( ME) topotype : 01
• Serotype O, South EastAsia ( SEA) topotype : 01
• Serotype O, Cathay topotype : 01
• Serotype A : 01
• Serotype Asia 1: 01

9
• Titrate these viruses
Setup SNT using the serum from vaccine trial on cattle as reference serum and to the
vaccine which was selected for applying in FMD National Control program since

2006.

3.4.5 RT-PCR application
Extract RNA from FMDV isolates
• Serotype O: 74 (52 from the South and 22 from the North)
• Serotype A: 9
• Serotype Asia 1: 4
Make cDNA from these RNA
Send cDNA and amplicons to AAHL for sequencing
The number of FMDV cDNA sequenced at AAHL:
• Serotype Asia 1: 4
• Serotype A: 9
• Serotype O: 55 (52 from the South and 03 from the North)

Report by

Ngo Thanh Long

Note: Further detail on activities for reporting period in log frame.

5.2 Smallholder Benefits
All pig and cattle and producers are potential beneficiaries. Those that take up the advice and
use vaccine according to recommendations will benefit financially through reduced losses
due to death and disease in their cattle and pigs. The benefits of a more profitable farming
operation flow to all family members. Farmers and district veterinarians will have improved
knowledge and skill in disease prevention, knowledge on the selection of the right vaccine
and improving the efficacy of vaccination
5.3 Capacity Building
Training and education of field veterinarians in disease prevention, disease investigation and
sample collection has continued with lessons learnt from the first round of sero-surveillance

and sample collection. This training has already shown an impact with an increase in quality
of sample collection and number of samples collected and submitted to the laboratory. The
project has provided training and technology transfer of FMD diagnostics to each laboratory
involved in the project. Reagents and standard methods have been supplied to each
laboratory giving them the diagnostic capability for FMDV diagnosis and serology using
ELISA technology, this technology is now being practised at each laboratory. The RAHC-
HCMC has virus isolation and cell culture techniques established and has begun to use
molecular technology and serology by VNT using cell culture. RAHC-HCMC now has
capacity to carry out RT-PCR for FMD for detection of FMD antigen and for sequence
analysis.

5.4 Publicity
The CARD AusAID project have received publicity through the training programs and also
through the achievements so far in the project in understanding FMD in Vietnam. FMD is a

10
disease on importance in Vietnam and this has put our project into the lime light. The project
has been publicised through a press release in Australia and articles in news letters including
the SEAFMD newsletter and on the internet. The results from the project have been
presented at OIE/SEAFMD meetings during the project.
5.5 Project Management
Implementation of the project is on schedule. AAHL has kept the project on target and has
supported or requests from the Vietnamese partners. Training has been carried out in
Australia and Vietnam with both partners benefiting from the information that is being
generated.
Implementation in Vietnam is also continuing to go well. Trainees have been supplied in a
timely manner, from DAH. The field work has been organised well with data and samples
being collected from farms in each pilot zone. Training and the transfer of information to
other regional laboratories in Vietnam is also occurring, RAHC-HCMC is supplying support
to these laboratories.

6. Report on Cross-Cutting Issues
6.1 Environment
No direct environmental impacts associated with project activities have been identified. On a
broader scale, the intention is that pig and cattle farming will become more efficient through
a reduction in animal death and disease. Environmental benefit will result through more
efficient utilization of resources such as animal feed, the energy to provide animal feed and
to run other aspects of the farm.
6.2 Gender and Social Issues
Application of new diagnostic tests will improve the ability of regional and provincial
diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and
appropriate measures to be applied to control disease. These benefits will begin during the
project and continue to accrue with continued application. The beneficiaries of this project
will be both large and small farms and particularly smallholder farmers whose animals and
incomes will be protected by better disease diagnosis, management and control. Since
women at the village level are the primary animal handlers and managers, they will be major
beneficiaries of the final outcome of better diagnosis and control of animal diseases.
7. Implementation & Sustainability Issues
7.1 Issues and Constraints
DAH continues to spend time controlling the AI outbreaks in Vietnam and this has made
there workload high. The collection of samples continues to be made more difficult by the
need to train all veterinarians in each province in data collection and on how to collect
samples, collection of blood and how to restrain cattle. This increased training need has
highlighted the need for further training of field veterinarians throughout Vietnam in disease
investigation techniques with a focus on sample collection and data collection. There is a
need for further input in this area, large scale training of field veterinarians to ensure better
knowledge and control of disease in Vietnam. This could be achieved though better
collaboration of aid agencies in Vietnam especially those supplying training for AI. Training
in data analysis by DAH staff is also an area that needs further input.

11

7.2 Options
The government of Vietnam is looking at increasing the support to DAH and has increased
funding for AI diagnosis and is looking to do the same for FMD diagnosis. The increased
funding for AI has seen laboratories updated with new equipment some of which will
improve all disease diagnosis which includes FMD.
The money available to improve AI diagnosis can improve all disease diagnosis if this money
is used wisely, eg in training field veterinarians in AI diagnosis, the training should be
general to cover all diseases including FMD, CSF and other diseases of importance in
Vietnam. Also in improving AI diagnosis in laboratories the training should emphasize how
improvement can be applied to all areas of diagnosis, eg quality assurance in the laboratory.

7.3 Sustainability
The DAH laboratories appear to be well supported by the central government. The DAH
laboratories are also receiving increased support from overseas funding agencies as part of
the AI campaign to improve laboratory facilities.
RAHC-HCMC is gaining in confidence as an institution. This confidence is beginning to be
passed on to other DAH laboratories. The DAH scientific staff are quick to understand new
information and are energetic in its implementation. People have an obvious desire to do
their jobs well. This desire is obvious in staff in RAHC-HCMC and other laboratories. The
laboratories just need technical support to be encouraged to carry out the techniques that they
need for diagnosis and research.
The project is following a well tried model for successful technology transfer, of
demonstration and teaching of the technology in the donor facility followed by supply of
reagents for implementation by the trainees at their own initiative in the recipient laboratory,
subsequently backed up by consultancy visits to the recipient laboratory for support and fine
tuning. A useful indicator of likely success is the initiative and ability of the trainees to make
a useful attempt at implementation by themselves. DAH and NAVETCO staff show a high
level of involvement in this way. The RAHC-HCMC now has established ELISA, cell
culture and molecular technologies.
The AusAID funded CSFV project, that linked DAH, NaVetCo and AAHL prior to this

project, is proving sustainable. NAVETCO and DAH not only maintains the diagnostic
capability developed at that time, but on its own initiative and unassisted by AAHL is
conducting training courses and technology transfer to other labs in Vietnam.
8. Next Critical Steps
In the next 6 months AAHL staff will review the project outputs and to examine the
information collected so far in the project and continue to provide support to the
establishment of the technologies transferred to the laboratories. The next round of the sero-
surveillance for the collection of the samples will be implemented with any changes
necessary. In the next 6 months we will finalise the transfer of technologies for FMD
diagnosis and evaluate each laboratories capability to carry out FMD diagnosis. HCMC-
RAHC will continue to use cell culture techniques to grow FMD isolates from the field. The
FMD isolates from the field will be send for further analysis of these isolates by PCR and
sequencing. Two trainees will visit AAHL to be trained in virus isolation and cell culture.
AAHL consultants to visit the laboratories to examine data from project and to review the
techniques for FMD diagnosis.

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9. Conclusion
The project activities are ahead of schedule with training, this will allow more time for the
laboratories in the project to take up the technology. The DAH laboratories are running the
field trials and have provided further training to the field staff to improve sample and data
collection from the field. The sero-surveillance is in progress and is expected to be completed
on schedule in the south but the north are finding it difficult to collect samples from the field
to due to the AI workload. The field veterinarians need further training in the collection of
data and how to ensure the correct information is obtained from the farmer. The staff at
RAHC-HCMC will continue to deal directly with the veterinarians in the field to ensure the
correct information is collected. Training of the HCMCHCMC laboratory in molecular
technology was completed, with a training course for 2 trainees from the laboratory held at
AAHL. RAHC-HCMC continues to carry out in country training where necessary to ensure
problems are addressed with the transfer of technology to the laboratories in the project. The

establishment of cell culture has allowed the growth of FMD isolates from the field that
could not be previously identified. The growth of isolates has also allowed sending of higher
quality samples to the world reference laboratory at Pirbright, UK, and allowing results to be
returned in a quicker timeframe. The introduction of molecular techniques at RAHC-HCMC
has allowed sequecing results to be obtained in real time allowing DAH Vietnam to make
informed decisions about serotypes of FMD circulating in Vietnam and the correct vaccine to
use in each region to control FMD.
This project continues to be important project for Vietnam with the importance of FMD
increasing due to the large outbreaks that have occurred in Vietnam in 2006.


13
Project Progress Against Proposed Objectives, Outputs, Activities And Inputs
Project Title:
Vietnamese Implementing Institution:
PROPOSAL PROGRESS REPORT
Narrative Information Required Performance Measures Assumptions Information Required
OBJECTIVES
1.






To establish an effective laboratory
network for the diagnosis and control
of FMD by the provision of resources
and training of staff in required
methods and quality assurance.

(Objective 1.2.2, 2.1.1 & Strategy 1)


Samples collected and
submitted to laboratory. Data
produced and results feedback
to field veterinary staff and
farms.

Technology will be
taken up by
laboratories. Accurate
data collected from
farms. Little risk.


Establishment of an effective laboratory network
for the diagnosis and control of FMD continued
with training given at AAHL under a Quality
system, method established at each laboratory, QA
introduced and resource supplied. The four DAH
laboratories are working together to carry out
surveillance and testing.

1.1

Train field veterinary staff in
outbreak investigation for the
investigation of cases of FMD on
farms, including record keeping,

sampling strategies and data
collection.



Samples collected from field
and submitted to laboratory.
Sample and farm records
presented with samples.

Accurate data collected
from farms. Little risk

Training continued to be carried out in the North,
Centre and South of Vietnam for veterinary field
and laboratory staff. Training is continuing for the
remainder of the project to ensure improved data
collection

1.2

Establish ELISA technology from
AAHL to collaborating laboratories
Diagnostics test in use. QA
controls meet. internal quality
control sheets for examination,
correct results in external
proficiency testing organized
by AAHL. Results generated.

Technology will be
taken up by
laboratories. Little risk

ELISA training and reagents delivered.
Laboratories have used the ELISA technology to
test samples from serosurveillance.

Narrative Information Required Performance Measures Assumptions Information Required

1.3

Using cell culture at RAHC-HCMC,

Virus isolates grown in cell

Cell cultures can be

Training at AAHL carried out and cells transferred

14

make a collection of isolates of FMD
viruses from cases of FMD disease
on farms.

culture maintained at RAHC-
HCMC. Little risk.

to RAHC-HCMC. FMD samples collected.
Further training in HCMC this 6 months and cell
culture established at RAHC-HCMC.
Cell culture used to isolate FMD virus from the
field in HCMC.

1.4

At RAHC-HCMC and AAHL use
molecular techniques to assess strains
of FMD circulating




Sequence data produced.

Technology will be
taken up by
laboratories. Little risk

FMD samples collected. FMD molecular
technology transfer has begun with first samples
sent to AAHL. Training in molecular techniques
and sequencing carried out at AAHL.

1.5

Introduce cell culture to NVDC-
Hanoi for FMD diagnosis

Cell culture in use at NVDC-
Hanoi

Technology will be
taken up by
laboratories. Little risk

Activity later in project

1.6

Firm link with AAHL for the
ongoing supply of diagnostic
reagents.

Diagnostic reagents used for
routine diagnostics.

Reagents available.
Little risk.




Process begun. Training in growth of virus for
ELISA antigen was started

2.

To provide accurate data to explain
failure of vaccination to control
FMDV and to develop new effective
vaccine application strategies.
(Objective 1.2.2, 2.1.1 & Strategy 1)



Project Report presented to
DAH and AusAID. Feedback
meetings held in provinces.

Project objectives meet.
Little risk.

FMD samples collected. Testing of samples for
phase 1 and 2 has been completed. Data collected
was not ideal and further training implemented to
improve data collection from the field.

Narrative Information Required Performance Measures Assumptions Information Required



15
2.1

2.2





2.3




2.4


2.5




2.6

Hold information and training
workshops for farmers and their
veterinary advisors, with emphasis
on FMD prevention and disease
investigation and sample collection


Pilot zones established in each
province.




Feedback to collaborating
laboratories results from test
comparisons at AAHL and RAHC-
HCMC.

Feedback results to veterinary field
staff and farmers.

Determine the efficacy of the FMD
vaccine in protecting against FMD
isolates circulating in Vietnam.

Hold information and training
workshops farmers and their
veterinary advisors, with emphasis
on FMD prevention and presenting
the data obtained during the CARD

project.

Samples collected from field
and submitted to laboratory.
Sample and farm records
presented with samples.



Samples collected from field
and submitted to laboratory.
Sample and farm records
presented with samples.



Results on laboratory
comparisons and EQA send
back to each laboratory.

Results maintained at field
veterinary office

Comparison of FMD
genotypes to vaccine. Results
of sero-surveillance.


Meetings held with farmers
and their veterinary advisors

Accurate data collected
from farms. Little risk





DAH is able to have
farmers agree to
project. Little risk



Technology will be
taken up by
laboratories. Little risk

Samples collected and
tested. Little risk.

Samples collected and
tested. Little risk.



Project will achieve
objectives.
Little risk.

Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff. Further information to farmers to
be delivered later in project. Feedback on first
phase of sero-surveillance to field veterinarians.
Further training given to improve data collection.

Pilot zones established. First round of sero-
surveillance completed. Second round of sero-
surveillance completed.



Feedback process has begun with laboratories
comparing test results. ELISA technology
established at RAHC HCMC and Hanoi.

Results of first round of sero-surveillance fed back
to field veterinarian staff.


Samples and data collected. Vaccination is low
and improved vaccination and data collection is
needed. DAH implementing FMD control plan.
Samples and information collected and is being
compiled



16

OUTPUTS
1. & 2.

• Field veterinarians that are
skilled in on farm diagnosis and
sample collection.




• Education of farmers to allow
better understanding of the
benefits of diagnostic
technologies.
• Development of cell culture
capability for virus propagation,
isolation from field samples and
for serology by virus
neutralisation test (VNT).
• Diagnostic capabilities at all the
laboratories participating in the
project to carry out functional
tests as described under a
Quality System.

• Pilot zones established and
operational.

• Firm link with AAHL for the
ongoing supply of diagnostic
reagents.
• Application of technologies,
knowledge and skills to other
diseases.

Samples collected and
submitted to laboratory.





Data produced and results
feedback to field veterinary
staff and farms.

Technology will be
taken up by
laboratories.




Accurate data collected
from farms. Little risk.

Process began. Training carried out in the North,
Centre and South of Vietnam for veterinary field
and laboratory staff. Information fed back to field
staff from first round of sero-surveillance to
improve sample and data collection.

Activity later in project

Training at AAHL carried out and cells transferred
to RAHC-HCMC. FMD samples collected. Cell
culture established at RAHC-HCMC. Virus
isolation carried out on samples from the field.
HCMC has started to use cell culture for serology.


Establishment of an effective laboratory network
for the diagnosis and control of FMD began with
training given, method established, QA introduced
and resource supplied. ELISA technology
transferred to each laboratory and testing of second
round of sero-surveillance ia complete. Diagnostic
tests established at RAHC-HCMC.
Pilot zones established. First round of sero-
surveillance completed. Second round of sero-
surveillance completed.

Process began.


Activity later in project


Expected outcome at end of project

17







1.1-1.6 &
2.1- 2.6







• Increased productivity by small
farm holders with more food
available to poor farmers. This
will have a more pronounced
effect on the women and
children who are mainly
responsible for the animals’
upkeep and likely to obtain the
major benefits of increased crop
production and sale of animals.

• Field veterinarians trained in on
farm diagnosis and sample
collection.



• Education of farmers to allow
better understanding of the
benefits of diagnostic
technologies to disease control.

• Setup farms/villages in each
province for seroservalance




• Transfer of ELISA technology to
collaborating laboratories

Samples collected from field
and submitted to laboratory.
Sample and farm records
presented with samples.


Farmers participating in
projects




Samples collected from field
and submitted to laboratory.
Sample and farm records
presented with samples.

Diagnostics test in use. QA
controls met, internal quality
control sheets available for
examination, correct results in
external proficiency testing
organized by AAHL. Results

Accurate data collected
from farms. Little risk



DAH is able to have
farmers agree to
project. Little risk



DAH is able to have
farmers agree to
project. Little risk

Technology will be
taken up by
laboratories. Little risk















Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff. Information fed back to field staff
from first round of sero-surveillance to improve
sample and data collection.

Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.

Pilot zones established. First round of sero-
surveillance completed. Second round of sero-
surveillance has been completed.




Establishment of an effective laboratory network
for the diagnosis and control of FMD began with
training given, methods established, QA
introduced and resource supplied. ELISA
technology transferred to each laboratory and
testing of second round of sero-surveillance is

18



• ELISA established in each
laboratory and EQA carried out
in each laboratory


• Transfer cell culture capability
to RAHC-HCMC


• Cell culture used to grow FMDV
from field isolates

• Transfer molecular techniques
for FMDV RT-PCR to RAHC-
HCMC

• Train RAHC-HCMC staff in
sequence analysis of sequenced
FMD isolates

• Train NVDC in use of cell
culture for FMD diagnosis

• Communication of diagnostic
results to field veterinarians and
farmers

• Recommendations on efficacy of
FMD vaccines

• Comparison of FMD isolates
generated.


Results on laboratory
comparisons and EQA send
back to each laboratory.


Virus isolates grown in cell
culture.

FMDV isolates sequenced



PCR products send to AAHL



Sequence data produced.




Trainee at AAHL from
NVDC-Hanoi


Results maintained at field
veterinary offices

Comparison of FMD
genotypes to vaccine.

Results of serosurveillance.



Technology will be

taken up by
laboratories. Little risk

Cell cultures can be
maintained at RAHC-
HCMC. Little risk.

Cell cultures can be
maintained at RAHC-
HCMC. Little risk.

Technology will be
taken up by
laboratories. Little risk

PCR products
submitted to AAHL




Trainee does not attend
training. Little risk


Samples collected and
tested. Little risk.

Samples collected and
tested. Little risk.

FMD Isolates collected.
complete. Diagnostic tests established at RAHC-
HCMC.

Process began. Diagnostic tests established at
RAHC-HCMC.



Training at AAHL carried out and cells transferred
to RAHC-HCMC. Cell culture established at
RAHC-HCMC. Virus isolation carried out on
samples from the field.

FMD samples collected virus isolation carried out
on isolates from the field.


FMD molecular technology transferred to
HCMC.PCR products from first samples sent to
AAHL and sequenced

RAHC-HCMC staff trained in sequence anaylsis.
Activity later in project



Results from first round of sero-surveillance fed back to
field veterinarians

Activity later in project


Results being compiled from sequencing
information with data showing isolates circulating
in Vietnam.


19
circulating in Vietnam

• Reagents in use in DAH
laboratories and purchase of
reagents by DAH for ongoing
epidemiology studies and
serosurveillance.

• Final project report written
summarising results obtained
from project

• Report to DAH and results of
project to be published
Report on sequence analysis
of FMD isolates

Diagnostic reagents used for
routine diagnostics.

Meetings held with farmers
and their veterinary advisors.


Final reports written.

Little risk.


Reagents available.
Little risk



Project will achieve
objectives. Little risk.


Project will achieve
objectives. Little risk.

Reagents in use at DAH laboratories




Activity later in project

Activity later in project

ACTIVITIES
1. & 2.



















To establish an effective laboratory
network for the diagnosis and control
of FMD by the provision of resources
and training of staff in required

methods and quality assurance.



To provide accurate data to explain
failure of vaccination to control
FMDV and to develop new effective
vaccine application strategies.



.





Major activity/objective of
the project, to be completed
over the 3 years of the
project

Diagnostic capabilities
at all the laboratories
participating in the
project to carry out
functional tests as
described under a
Quality System.

Field veterinarians that
are skilled in on farm
diagnosis and sample
collection.
Education of farmers to
allow better
understanding of the
benefits of diagnostic
technologies.

Pilot zones established
and operational.

Establishment of an effective laboratory network

for the diagnosis and control of FMD continued
with training given at AAHL under a Quality
system, method established at each laboratory, QA
introduced and resource supplied. The four DAH
laboratories are working together to carry out
surveillance and testing.

Pilot zones established. First round of sero-
surveillance completed. Second round of sero-
surveillance has begun.











20

• Order and purchase equipment

• Supply laboratory consumables

• AAHL staff will prepare
information and lectures for
workshop for training and
education of field veterinarians
and farmers

• Run first workshop at RAHC-
HCMC field veterinarians

• Supply consumables and budget
for sample collection

• RAHC-HCMC to initiate
epidemiological and
serosurveillance programs


• RAHC-HCMC and NCDC-
Hanoi to coordinate training and
meetings with field veterinarians
in provinces where fields studies
are to be carried out.

First month year 1


First 3 months year 1


First 3 months year 1




First 3 months year 1


First 3 months year 1


First 3 months year 1



First 3-6 months year 1

Recommendations on
vaccine application
from epidemiological
and serosurveillance
data.

Equipment in place at
laboratories

Consumables at
laboratories

Workshop held at
RAHC-HCMC




Workshop held at
RAHC-HCMC

Consumables and
budget supplied

Contact made with field
veterianians to begin
sample collection for

disease outbreaks

Workshop & meetings
held












Equipment in place at laboratories


Consumables supplied to laboratories


Completed. Information supplied and used in
workshops




Training carried out in the North, Centre and
South of Vietnam for veterinary field and

laboratory staff.

Consumables and budget supplied


First phase of sero-surveillance complete and
second round in progress




Training carried out in the North, Centre and
South of Vietnam for veterinary field and
laboratory staff.

21
• Hold meetings to educate
farmers on the benefits of
epidemiological studies,
serosurveillance and laboratory
diagnostics to disease control

• Sampling schedule setup for
each province in the project



• Farms/village setup with
information on sampling
schedule and project requirement

• Costs of sampling negotiated
with farmers and field veterinary
staff

• Serosurveillance carried in each
province


• Samples collected from field and
tested in each laboratory


• Scientists from RAHC-HCMC
laboratories to be trained in
FMD diagnostics, ELISAs and
cell culture at AAHL.

• Follow up training by AAHL to
establish ELISA and cell culture
First 3-6 months year 1





First month year 1

First 3 months year 1





First 3 months year 1



Twice a year for 3 years



Twice a year for 3 years



First 3 months year 1




First 3 - 6 months year 1

Meetings held

Document produced for
sampling schedule


Document for project
field studies circulated
to filed veterinarians
and farmers

Payment details
completed



Samples collected and
send to laboratories

Results generated



Scientists trained at
AAHL

Visit by AAHL
consultant to RAHC-
HCMC




Initial contact made with farmers in project.
Education to be carried out as part of National
program




Sampling schedule documented during workshop
for Veterinarians. Pilot zones established. First
round of sero-surveillance completed. Second
round of sero-surveillance in progress.
Pilot zones established. First round of sero-
surveillance completed. Second round of sero-
surveillance in progress. Feedback on first phase
of sero-surveillance to field veterinarians.

Budget for field work received and transfer to field
staff.


First round of sero-surveillance completed. Second
round of sero-surveillance in progress.

FMD outbreak samples tested by ELISA. Testing
of samples for phase has been completed and
testing of second phase samples has begun.
Completed





22
techniques at RAHC-HCMC.

• Cell cultures and consumables
supplied to RAHC-HCMC

• Cell cultures grown and stored at
RAHC-HCMC

• Cell cultures used for virus
isolation and serology


• AAHL to run ELISA workshop
at RAHC-HCMC

• Follow training by RAHC-
HCMC staff to each laboratory

• FMD ELISAs established in

regional laboratories.


• Samples send from each
laboratory to RAHC-HCMC and
AAHL for comparison


• Accreditation of laboratory for
FMD ELISAs





• One scientist from each of the



First 3 - 6 months year 1


First 3 - 6 months year 1


7-8th month of year 1


First 6 months year 1

First 3 - 6 months year 1


First 6-9 months year 1




Twice a year for 3 years



First 6-9 months year 1






First 1 month year 2


Cell cultures &
consumables delivered

Cell cultures in use

FMDV isolated from
field SNT used for
serology

Workshop completed






ELISA working in each
laboratory



ELISA results send to
AAHL



Results of comparisons
reported back to
laboratories
ELISAs functional at
each laboratory, IQC
available

Completed

Completed




Completed. Cell culture established at RAHC-
HCMC.

Virus isolation carried out on samples from the
field. FMD isolates grown.



Completed



Completed


Laboratories have used the ELISA technology to
test samples. ELISA established at HCMC and
Hanoi. Diagnostic tests established at RAHC-
HCMC for FMD diagnostics
Samples have been send to RAHC-HCMC for
comparative testing and AAHL. Results indicate
that ELISA is established for use at RAHC-HCMC

Activity later in project. HCMC & Hanoi are
accredited.




23
regional laboratories trained in
ELISA techniques at AAHL.

• Visit by AAHL scientist to each
regional laboratory to check
ELISA operation and QA in the
laboratories

• Carry out EQA testing at each
laboratory



• Meeting to be held in Australia
with project leaders to discuss
progress of project, QA lectures
will be part of this visit

• Set up external QA program for
the regional laboratories at
RAHC-HCMC.

• RAHC-HCMC scientists trained
in molecular techniques

• PCR products FMD isolates in
Vietnam sent to AAHL from
RAHC-HCMC

• Sequencing on FMDV isolates
collected in Vietnam at AAHL

• AAHL staff to train regional
laboratory staff in analyzing data
from the field



First 3 months year 2




First 3 months year 2





Middle of Year2

First 3 months year 3



Third 3 months of Year 2


Second & Third year of
project

Second & Third year of
project


First 3 months year 3


Scientists trained at
AAHL


Visit by AAHL
consultant completed



EQA results collated
and returned to each

laboratory



Meeting





Carry out final round of
EQA


Scientists trained at
AAHL


PCR products received
at AAHL


Sequence data from
FMD isolates produced



Training brought forward and completed.

Laboratories running FMD ELISAs.



Reference sera from AAHL tested by laboratories.
ELISA is operating under QAz system. Meeting
held at AAHL with Director of DAH, Director of
RAHO-HCMC . Also held meeting in Canberra
wit DAFF. Project achieving aims. Activity later
in project



FMD molecular technology transfer has begun
with PCR products from first samples sent to
AAHL.

50 FMD isolates sequenced at AAHL.



Activity later in project


Activity later in project


Activity later in project

24

• One NVDC-Hanoi scientist
trained at AAHL in cell culture.

• Scientist from RAHC-HCMC to
be trained in cell culture and
virus isolation at AAHL

• Supply cells to HVDC-Hanoi

• Further workshops at regional
centres to reinforce disease
investigation protocols and to
provide feedback to
veterinarians and farmers on the
epidemiological and
serosurveillance data collected.
Review of project and
accomplishments.

• Finalise results from samples
from field outbreaks and report
back to field veterinarians and
farmers

• Finalise serology results and
report back to field veterinarians

and farmers

• Summarise results from
epidemiological studies and
serosurveillance from project

• Carry out sequencing of FMD
isolates collected during project

First 3 months year 3



First 3 months year 3


First 3 months year 3


Last 6 months year 3









Twice a year for 3 years

Twice a year for 3 years



Last 6 months year 3


Second & Third years of
project

AAHL staff to visit
Vietnam

Scientist trained at
AAHL



Scientist trained at
AAHL


Cell cultures received at
HVDC-Hanoi

Feedback to field

veterinarians and
farmers on project
results and
recommendations




Results reported to
DAH and farmers



Results reported to
DAH and farmers


Write report







First round of sero-surveillance completed and
reported back to filed veterinarians. Serotyping
carried out on field samples to confirm FMD.
FMD isolates sequenced. First round of sero-
surveillance completed and reported back to filed

veterinarians

Activity later in project



50 isolates sequenced



Activity later in project

Activity later in project

Activity later in project





25

• Write recommendations for use
of FMD vaccine

• Draft final project report

• Write final project reports for
CARD and present report to
MARD and DAH

Last 6 months year 3


Last 3 months year 3


Last 3 months year 3




Collate & report
sequence data from
project

Report
recommendations from
project

Final project meeting
held
Reports completed

INPUTS See attached statuary declaration and
above for project inputs