ADVANCES IN HEMATOPOIETIC STEM CELL RESEARCH pot

ADVANCES IN
HEMATOPOIETIC
STEM CELL RESEARCH

Edited by Rosana Pelayo
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Advances in Hematopoietic Stem Cell Research
Edited by Rosana Pelayo


Published by InTech
Janeza Trdine 9, 51000 Rijeka, Croatia

Copyright © 2011 InTech
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First published January, 2012
Printed in Croatia

A free online edition of this book is available at www.intechopen.com
Additional hard copies can be obtained from


Advances in Hematopoietic Stem Cell Research, Edited by Rosana Pelayo
p. cm.
978-953-307-930-1

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Contents
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Preface IX
Part 1 Hematopoietic Stem Cell Properties 1
Chapter 1 Networks Establishing Hematopoietic
Stem Cell Multipotency and Self-Renewal 3
Eliana Abdelhay, Luciana Pizzatti and
Renata Binato
Chapter 2 Regulation of Hematopoietic Stem Cell Fate:
Self-Renewal, Quiescence and Survival 39
Yasushi Kubota and Shinya Kimura
Chapter 3 Transcriptional Quiescence of Hematopoietic Stem Cells 61
Rasmus Freter
Chapter 4 Markers for Hematopoietic Stem Cells:
Histories and Recent Achievements 77
Takafumi Yokota, Kenji Oritani,
Stefan Butz, Stephan Ewers,
Dietmar Vestweber and Yuzuru Kanakura
Part 2 Regulation of Hematopoietic Stem Cells 89
Chapter 5 Interferon Regulatory Factor-2 Regulates
Hematopoietic Stem Cells in Mouse Bone Marrow 91
Atsuko Masumi, Shoichiro Miyatake,
Tomoko Kohno and Toshifumi Matsuyama
Chapter 6 Regulation of Tyrosine Kinase
Signaling by Cbl in Hematopoietic Stem Cells 113
Mayumi Naramura

Chapter 7 The Hypoxia Regulatory System in
Hematopoietic Stem Cells 133
Keiyo Takubo
VI Contents

Chapter 8 Skeletogenesis and the Hematopoietic Niche 147
Elizabeth Sweeney and Olena Jacenko
Chapter 9 Molecular Mechanisms Underlying Bone
Marrow Homing of Hematopoietic Stem Cells 185
Aysegul Ocal Sahin and Miranda Buitenhuis
Chapter 10 Searching for the Key to
Expand Hematopoietic Stem Cells 205
Jeanne Grosselin, Karine Sii-Felice,
Philippe Leboulch and Diana Tronik-Le Roux
Part 3 Hematopoietic Stem Cells in Aging and Disease 245
Chapter 11 Insights Into Stem Cell Aging 247
A. Herrera-Merchan, I. Hidalgo, L. Arranz and S. Gonzalez
Chapter 12 Hematopoietic Stem Cell in
Acute Myeloid Leukemia Development 261
Sérgio Paulo Bydlowski and Felipe de Lara Janz
Chapter 13 From HSC to B-Lymphoid Cells in
Normal and Malignant Hematopoiesis 277
Rosana Pelayo, Elisa Dorantes-Acosta,
Eduardo Vadillo and Ezequiel Fuentes-Pananá
Chapter 14 Distribution of SDF1-3’A, GNB3 C825T and MMP-9 C-1562T
Polymorphisms in HSC CD34+ from Peripheral Blood of
Patients with Hematological Malignancies 299
Ben Nasr Moufida and Jenhani Faouzi
Chapter 15 Hematopoietic Derived Fibrocytes:
Emerging Effector Cells in Fibrotic Disorders 317

Carolina García-de-Alba, Moisés Selman and Annie Pardo
Part 4 Hematopoietic Stem Cell Therapy 345
Chapter 16 Hematopoietic Stem Cells Therapeutic Applications 347
Carla McCrave
Chapter 17 Hematopoietic Stem Cell Potency for
Cellular Therapeutic Transplantation 383
Karen M. Hall, Holli Harper and Ivan N. Rich
Chapter 18 Detection of CMV Infection in
Allogeneic SCT Recipients: The Multiple Assays 407
Pilar Blanco-Lobo,
Omar J. BenMarzouk-Hidalgo and Pilar Pérez-Romero
Contents VII

Chapter 19 Bone Marrow Derived Pluripotent
Stem Cells in Ischemic Heart Disease: Bridging the
Gap Between Basic Research and Clinical Applications 425
Ahmed Abdel-Latif, Ewa Zuba-Surma and Mariusz Z. Ratajczak
Chapter 20 Gene Therapy of Hematopoietic and
Immune Systems: Current State and Perspectives 441
Maria Savvateeva, Fedor Rozov and Alexander Belyavsky




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Preface
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The prospective isolation of primitive blood-forming cells along with depicting of
transcriptional networks that control early cell fate decisions, and characterization of
microenvironmental signals influencing differentiation pathways during normal
hematopoiesis, have been critical to the construction of a hierarchical model for the
hematopoietic development, that has served as a paradigm for a number of systems
within vertebrate development. Hematopoietic stem cell research has been helpful to
elucidate mechanisms that govern tissue regeneration, to give an insight into
perspectives that may allow protection of the system during disease, to design life-
saving therapies, and to discover novel drug activities. This promising field is being
accelerated by significant contributions in genomics, molecular biology, and
technologies including fluorescent activated cell sorting and mouse engineering, that
give us a more integrated view of the nature of stem cells .
This book, Advances in Hematopoietic Stem Cell Research, is devoted to current and in-
progress scientific knowledge on basic aspects of these seminal cells and their
therapeutic applications. The text consists of 20 chapters grouped into four sections: 1)
Hematopoietic stem cell properties, 2) Regulation of hematopoietic stem cells, 3)
Hematopoietic stem cells in aging and disease, and 4) Hematopoietic stem cell
therapy.
The first section provides four comprehensive chapters on the functional
characteristics and biological properties that make distinctive the conspicuous
population of hematopoietic stem cells, including multipotency, self-renewal,
quiescence and novel surface markers. Five chapters in the second section contain
powerful information about intrinsic and extrinsic factors that determine cell fates in
early development. The authors have discussed cellular and molecular aspects of the
hematopoietic microenvironment within the bone marrow, and progress in searching
of procedures to make the expansion of truly stem cells possible . Behavior of the stem
and progenitor cells in aging and during disease is analyzed in the third section of this
text, which highlights recent achievements in unraveling the role of primitive cells in
the pathogenesis of hematological malignancies like leukemia.
Finally, to provide a comprehensive overview of the advancements in therapeutic

applications of hematopoietic stem cells, a fourth section with five chapters addresses
X Preface

a number of diseases for which stem cell transplantation is the indicated therapy. Of
special interest, has been the evaluation of quality and potency of stem and progenitor
cells for therapy purposes. Substantial efforts to assemble the bridge between basic
research and clinical applications are currently being recorded worldwide, and their
review in this section may increase the interest for the book.
It is hoped that Advances in Hematopoietic Stem Cell Research will prove to be an
enjoyable read and that it contributes to this area of Modern Medicine. This book is in
effect a compilation of the latest advances resulted from the participation of
distinguished and dedicated authors, experts in the field, to whom I am extremely
thankful. I would like to acknowledge the terrific contribution of Maja Bozicevic in the
professional editing of the book.
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Rosana Pelayo
Oncology Research Unit, Oncology Hospital,
Mexican Institute for Social Security, Mexico City,
Mexico



Part 1
Hematopoietic Stem Cell Properties