Luận văn : Nghiên cứu kỹ thuật phát sinh phôi soma và tạo hạt nhân tạo ở cây lan Hồ Điệp (Phalaenopsis sp. ) part 9 ppt

81


Thí nghiệm 3 : Ảnh hưởng của chất kích thích sinh trưởng TDZ trong điều
kiện môi trường đặc, lỏng lắc đến khả năng hình thành phôi soma từ mô sẹo của
cây lan Hồ Điệp in vitro

Số phôi tạo ra từ môi trường đặc


Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance


Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 188.77344 4 47.193360 38.747 .0000
Within groups 12.18000 10 1.218000

Total (corrected) 200.95344 14

0 missing value(s) have been excluded.



Table of means

Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 .000000 .0000000 .6371813 -1.0041673 1.004167
2 3 2.670000 .5773503 .6371813 1.6658327 3.674167
3 3 4.330000 .1732051 .6371813 3.3258327 5.334167
4 3 10.670000 .5773503 .6371813 9.6658327 11.674167
5 3 5.670000 1.1547005 .6371813 4.6658327 6.674167

Total 15 4.668000 .2849561 .2849561 4.2189227 5.117077


Multiple range analysis



Method: 95 Percent LSD
Level Count Average Homogeneous Groups

1 3 .000000 X
2 3 2.670000 X
3 3 4.330000 XX
5 3 5.670000 X
4 3 10.670000 X

contrast difference limits
1 - 2 -2.67000 2.00833 *
1 - 3 -4.33000 2.00833 *
1 - 4 -10.6700 2.00833 *
1 - 5 -5.67000 2.00833 *

2 - 3 -1.66000 2.00833
2 - 4 -8.00000 2.00833 *
2 - 5 -3.00000 2.00833 *
3 - 4 -6.34000 2.00833 *
* denotes a statistically significant difference.

82


Số phôi tạo ra từ môi trường lỏng lắc



Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance



Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 2931.9868 4 732.99669 261.039 .0000
Within groups 28.0800 10 2.80800

Total (corrected) 2960.0668 14

0 missing value(s) have been excluded.

Table of means



Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 .000000 .0000000 .9674709 -1.524688 1.524688
2 3 4.330000 .5773503 .9674709 2.805312 5.854688
3 3 12.670000 .1154701 .9674709 11.145312 14.194688
4 3 39.670000 1.1547005 .9674709 38.145312 41.194688
5 3 20.000000 1.7320508 .9674709 18.475312 21.524688

Total 15 15.334000 .4326662 .4326662 14.652139 16.015861




Multiple range analysis


Method: 95 Percent LSD
Level Count Average Homogeneous Groups

1 3 .000000 X
2 3 4.330000 X
3 3 12.670000 X
5 3 20.000000 X
4 3 39.670000 X

contrast difference limits
1 - 2 -4.33000 3.04938 *
1 - 3 -12.6700 3.04938 *
1 - 4 -39.6700 3.04938 *
1 - 5 -20.0000 3.04938 *
2 - 3 -8.34000 3.04938 *
2 - 4 -35.3400 3.04938 *
2 - 5 -15.6700 3.04938 *
3 - 4 -27.0000 3.04938 *
* denotes a statistically significant difference.





83



Thí nghiệm 4 : Ảnh hưởng của các loại môi trường đến quá trình tái sinh
cây lan Hồ Điệp in vitro từ phôi soma

Tỉ lệ mẫu chết


Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance

Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 2481.8963 7 354.55662 47.817 .0000
Within groups 118.6371 16 7.41482

Total (corrected) 2600.5334 23

0 missing value(s) have been excluded.


Table of means


Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 26.670000 .0000000 1.5721341 24.312787 29.027213
2 3 24.446667 2.2233333 1.5721341 22.089454 26.803879
3 3 4.446667 2.2233333 1.5721341 2.089454 6.803879
4 3 2.223333 2.2233333 1.5721341 133879 4.580546
5 3 6.670000 .0000000 1.5721341 4.312787 9.027213
6 3 2.223333 2.2233333 1.5721341 133879 4.580546
7 3 .000000 .0000000 1.5721341 -2.357213 2.357213
8 3 .000000 .0000000 1.5721341 -2.357213 2.357213

Total 24 8.335000 .5558333 .5558333 7.501599 9.168401

Multiple range analysis


Method: 95 Percent LSD
Level Count Average Homogeneous Groups

7 3 .000000 X
8 3 .000000 X
4 3 2.223333 XX
6 3 2.223333 XX
3 3 4.446667 XX
5 3 6.670000 X
2 3 24.446667 X
1 3 26.670000 X

contrast difference limits
1 - 2 2.22333 4.71443
1 - 3 22.2233 4.71443 *
1 - 4 24.4467 4.71443 *
1 - 5 20.0000 4.71443 *
1 - 6 24.4467 4.71443 *
* denotes a statistically significant difference.
84



Hệ số nhân chồi

Means plot: LSD Confidence level: 95 Range test: LSD



Analysis of variance


Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 381.55196 7 54.507423 78.569 .0000
Within groups 11.10000 16 .693750

Total (corrected) 392.65196 23

0 missing value(s) have been excluded.


Table of means



Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 .000000 .0000000 .4808846 721025 .721025
2 3 .780000 .0577350 .4808846 .058975 1.501025
3 3 1.440000 .2309401 .4808846 .718975 2.161025
4 3 9.000000 1.1547005 .4808846 8.278975 9.721025
5 3 .440000 .1154701 .4808846 281025 1.161025
6 3 3.000000 .2886751 .4808846 2.278975 3.721025

7 3 11.340000 .1732051 .4808846 10.618975 12.061025
8 3 5.330000 .5773503 .4808846 4.608975 6.051025

Total 24 3.916250 .1700184 .1700184 3.661329 4.171171




Multiple range analysis



Method: 95 Percent LSD
Level Count Average Homogeneous Groups

1 3 .000000 X
5 3 .440000 X
2 3 .780000 X
3 3 1.440000 X
6 3 3.000000 X
8 3 5.330000 X
4 3 9.000000 X
7 3 11.340000 X

contrast difference limits
1 - 2 -0.78000 1.44205
1 - 3 -1.44000 1.44205
1 - 4 -9.00000 1.44205 *
1 - 5 -0.44000 1.44205
1 - 6 -3.00000 1.44205 *

* denotes a statistically significant difference.


85


Số lá/chồi


Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance


Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 3.9647625 7 .5663946 462.363 .0000
Within groups .0196000 16 .0012250

Total (corrected) 3.9843625 23

0 missing value(s) have been excluded.


Table of means



Stnd. Error Stnd. Error 95 % LSD

Level Count Average (internal) (pooled s) intervals for mean

1 3 .0000000 .0000000 .0202073 0302982 .0302982
2 3 1.0000000 .0000000 .0202073 .9697018 1.0302982
3 3 1.0000000 .0000000 .0202073 .9697018 1.0302982
4 3 1.2300000 .0173205 .0202073 1.1997018 1.2602982
5 3 1.0000000 .0000000 .0202073 .9697018 1.0302982
6 3 1.2300000 .0346410 .0202073 1.1997018 1.2602982
7 3 1.3800000 .0115470 .0202073 1.3497018 1.4102982
8 3 1.2700000 .0404145 .0202073 1.2397018 1.3002982

Total 24 1.0137500 .0071443 .0071443 1.0030380 1.0244620



Multiple range analysis




Method: 95 Percent LSD
Level Count Average Homogeneous Groups

1 3 .0000000 X
2 3 1.0000000 X
3 3 1.0000000 X
5 3 1.0000000 X
6 3 1.2300000 X
4 3 1.2300000 X
8 3 1.2700000 X

7 3 1.3800000 X

contrast difference limits
1 - 2 -1.00000 0.06060 *
1 - 3 -1.00000 0.06060 *
1 - 4 -1.23000 0.06060 *
1 - 5 -1.00000 0.06060 *
1 - 6 -1.23000 0.06060 *
* denotes a statistically significant difference.




86



Số rễ/chồi



Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance

Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups .2386500 7 .0340929 136.371 .0000
Within groups .0040000 16 .0002500

Total (corrected) .2426500 23

0 missing value(s) have been excluded.


Table of means


Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 .0000000 .0000000 .0091287 0136873 .0136873
2 3 .0000000 .0000000 .0091287 0136873 .0136873
3 3 .0000000 .0000000 .0091287 0136873 .0136873
4 3 .0000000 .0000000 .0091287 0136873 .0136873
5 3 .0000000 .0000000 .0091287 0136873 .0136873
6 3 .2400000 .0230940 .0091287 .2263127 .2536873
7 3 .2200000 .0115470 .0091287 .2063127 .2336873
8 3 .0000000 .0000000 .0091287 0136873 .0136873

Total 24 .0575000 .0032275 .0032275 .0526608 .0623392



Multiple range analysis



Method: 95 Percent LSD

Level Count Average Homogeneous Groups

1 3 .0000000 X
2 3 .0000000 X
3 3 .0000000 X
4 3 .0000000 X
5 3 .0000000 X
8 3 .0000000 X
7 3 .2200000 X
6 3 .2400000 X

contrast difference limits
1 - 2 0.00000 0.02737
1 - 3 0.00000 0.02737
1 - 4 0.00000 0.02737
1 - 5 0.00000 0.02737
1 - 6 -0.24000 0.02737 *
* denotes a statistically significant difference.





87


Chiều dài rễ

Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance


Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 66.345863 7 9.4779804 60.659 .0000
Within groups 2.500000 16 .1562500

Total (corrected) 68.845863 23

0 missing value(s) have been excluded.



Table of means



Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 .0000000 .0000000 .2282177 3421831 .3421831
2 3 .0000000 .0000000 .2282177 3421831 .3421831
3 3 .0000000 .0000000 .2282177 3421831 .3421831
4 3 .0000000 .0000000 .2282177 3421831 .3421831
5 3 .0000000 .0000000 .2282177 3421831 .3421831
6 3 3.6700000 .5773503 .2282177 3.3278169 4.0121831

7 3 4.0000000 .2886751 .2282177 3.6578169 4.3421831
8 3 .0000000 .0000000 .2282177 3421831 .3421831

Total 24 .9587500 .0806872 .0806872 .8377700 1.0797300




Multiple range analysis



Method: 95 Percent LSD
Level Count Average Homogeneous Groups

1 3 .0000000 X
2 3 .0000000 X
3 3 .0000000 X
4 3 .0000000 X
5 3 .0000000 X
8 3 .0000000 X
6 3 3.6700000 X
7 3 4.0000000 X

contrast difference limits
1 - 2 0.00000 0.68437
1 - 3 0.00000 0.68437
1 - 4 0.00000 0.68437
1 - 5 0.00000 0.68437
1 - 6 -3.67000 0.68437 *

* denotes a statistically significant difference.


88

Thí nghiệm 5 : Ảnh hưởng của môi trường tạo vỏ hạt đến sự tái sinh cây con từ hạt
nhân tạo


Means plot: LSD Confidence level: 95 Range test: LSD


Analysis of variance


Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level

Between groups 4434.6323 5 886.92645 126.704 .0000
Within groups 84.0000 12 7.00000

Total (corrected) 4518.6323 17

0 missing value(s) have been excluded.


Table of means


Stnd. Error Stnd. Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean

1 3 44.440000 1.1547005 1.5275252 42.086005 46.793995
2 3 77.780000 1.1547005 1.5275252 75.426005 80.133995
3 3 37.780000 1.1547005 1.5275252 35.426005 40.133995
4 3 55.560000 .5773503 1.5275252 53.206005 57.913995
5 3 71.110000 2.8867513 1.5275252 68.756005 73.463995
6 3 37.780000 1.1547005 1.5275252 35.426005 40.133995

Total 18 54.075000 .6236096 .6236096 53.113986 55.036014



Multiple range analysis


Method: 95 Percent LSD
Level Count Average Homogeneous Groups

3 3 37.780000 X
6 3 37.780000 X
1 3 44.440000 X
4 3 55.560000 X
5 3 71.110000 X
2 3 77.780000 X

contrast difference limits
1 - 2 -33.3400 4.70799 *
1 - 3 6.66000 4.70799 *
1 - 4 -11.1200 4.70799 *
1 - 5 -26.6700 4.70799 *

1 - 6 6.66000 4.70799 *
2 - 3 40.0000 4.70799 *
2 - 4 22.2200 4.70799 *
* denotes a statistically significant difference.

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