Multiple range analysis for DR14DAY.DAIRE by DR14DAY.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
1 27 2.2222222 X
2 27 7.5925926 X
contrast difference +/- limits
1 - 2 -5.37037 0.29520 *
* denotes a statistically significant difference.
21 ngày
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 4.166667 1 4.1666667 7.927 .0069
Within groups 27.333333 52 .5256410
Total (corrected) 31.500000 53
0 missing value(s) have been excluded.
Multiple range analysis for DR21DAY.DAIRE by DR21DAY.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
1 27 5.2222222 X
2 27 5.7777778 X
contrast difference limits
1 - 2 0.55556 0.39605 *
* denotes a statistically significant difference.
28 ngày
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 266.66667 1 266.66667 216.166 .0000
Within groups 64.14815 52 1.23362
Total (corrected) 330.81481 53
0 missing value(s) have been excluded
Multiple range analysis for DR28DAY.DAIRE by DR28DAY.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
1 27 5.629630 X
2 27 10.074074 X
contrast difference +/- limits
1 - 2 -4.44444 0.60673 *
* denotes a statistically significant difference.
35 ngày
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 317.79630 1 317.79630 157.108 .0000
Within groups 105.18519 52 2.02279
Total (corrected) 422.98148 53
0 missing value(s) have been excluded.
Multiple range analysis for DR35DAY.daire by DR35DAY.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
1 27 7.592593 X
2 27 12.444444 X
contrast difference limits
1 - 2 -4.85185 0.77692 *
* denotes a statistically significant difference.
4. XỬ LÝ SỐ LIỆU CÂY TRUỜNG XUÂN HOA IN VITRO TRÊN MÔI TRƯỜNG CHỨA NAA
CAO CÂY TRÊN MOI TRUƠNG CÓ NAA
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 1124.938 5 224.98765 2.785 .0194
Within groups 12603.704 156 80.79297
Total (corrected) 13728.642 161
0 missing value(s) have been excluded.
Multiple range analysis for CCNAA.Caocay by CCNAA.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
6 27 49.296296 X
5 27 49.518519 X
2 27 51.814815 XX
1 27 51.851852 XX
3 27 55.666667 X
4 27 55.925926 X
contrast difference limits
1 - 2 0.03704 4.83333
1 - 3 -3.81481 4.83333
1 - 4 -4.07407 4.83333
1 - 5 2.33333 4.83333
1 - 6 2.55556 4.83333
2 - 3 -3.85185 4.83333
2 - 4 -4.11111 4.83333
2 - 5 2.29630 4.83333
2 - 6 2.51852 4.83333
3 - 4 -0.25926 4.83333
3 - 5 6.14815 4.83333 *
3 - 6 6.37037 4.83333 *
4 - 5 6.40741 4.83333 *
4 - 6 6.62963 4.83333 *
5 - 6 0.22222 4.83333
* denotes a statistically significant difference.
SỐ RỄ TRÊN MOI TRUƠNG CÓ NAA
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 216.27160 5 43.254321 147.401 .0000
Within groups 45.77778 156 .293447
Total (corrected) 262.04938 161
0 missing value(s) have been excluded.
Multiple range analysis for SRNÁA.SORE by SRNAA.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
6 27 .6666667 X
5 27 1.9259259 X
1 27 2.9259259 X
2 27 3.0000000 X
4 27 3.6666667 X
3 27 4.1851852 X
contrast difference limits
1 - 2 -0.07407 0.29129
1 - 3 -1.25926 0.29129 *
1 - 4 -0.74074 0.29129 *
1 - 5 1.00000 0.29129 *
1 - 6 2.25926 0.29129 *
2 - 3 -1.18519 0.29129 *
2 - 4 -0.66667 0.29129 *
2 - 5 1.07407 0.29129 *
2 - 6 2.33333 0.29129 *
3 - 4 0.51852 0.29129 *
3 - 5 2.25926 0.29129 *
3 - 6 3.51852 0.29129 *
4 - 5 1.74074 0.29129 *
4 - 6 3.00000 0.29129 *
5 - 6 1.25926 0.29129 *
* denotes a statistically significant difference. .
DÀI RỄ TRÊN MOI TRUỜNG CÓ NAA
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 462.86420 5 92.572840 187.280 .0000
Within groups 77.11111 156 .494302
Total (corrected) 539.97531 161
0 missing value(s) have been excluded.
Multiple range analysis for DRNAA.DAIRE by DRNAA.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
6 27 1.1111111 X
5 27 2.4444444 X
1 27 4.1851852 X
2 27 5.2222222 X
3 27 5.4814815 XX
4 27 5.6296296 X
contrast difference +/- limits
1 - 2 -1.03704 0.37806 *
1 - 3 -1.29630 0.37806 *
1 - 4 -1.44444 0.37806 *
1 - 5 1.74074 0.37806 *
1 - 6 3.07407 0.37806 *
2 - 3 -0.25926 0.37806
2 - 4 -0.40741 0.37806 *
2 - 5 2.77778 0.37806 *
2 - 6 4.11111 0.37806 *
3 - 4 -0.14815 0.37806
3 - 5 3.03704 0.37806 *
3 - 6 4.37037 0.37806 *
4 - 5 3.18519 0.37806 *
4 - 6 4.51852 0.37806 *
5 - 6 1.33333 0.37806 *
* denotes a statistically significant difference.
.
5. XỬ LÝ SỐ LIỆU CÂY TRUỜNG XUÂN HOA IN VITRO TRÊN MÔI TRƯỜNG CHỨA IAA
CAO CÂY TRÊN MOI TRUƠNG CÓ IAA
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 560.9938 5 112.19877 2.637 .0256
Within groups 6638.3704 156 42.55366
Total (corrected) 7199.3642 161
0 missing value(s) have been excluded.
Multiple range analysis for CCIAA.CAOCAY by CCIAA.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
6 27 46.333333 X
5 27 48.740741 XX
4 27 49.407407 XX
3 27 50.407407 X
2 27 51.518519 X
1 27 51.851852 X
contrast difference limits
1 - 2 2.44444 3.50775
1 - 3 1.44444 3.50775
1 - 4 0.33333 3.50775
1 - 5 3.11111 3.50775
1 - 6 5.51852 3.50775 *
2 - 3 -1.00000 3.50775
2 - 4 -2.11111 3.50775
2 - 5 0.66667 3.50775
2 - 6 3.07407 3.50775
3 - 4 -1.11111 3.50775
3 - 5 1.66667 3.50775
3 - 6 4.07407 3.50775 *
4 - 5 2.77778 3.50775
4 - 6 5.18519 3.50775 *
5 - 6 2.40741 3.50775
* denotes a statistically significant difference * denotes a
statistically significant difference.
SỐ RỄ TRÊN MOI TRUƠNG CÓ IAA
One-Way Analysis of Variance
Data: SRIAA.SORE
Level codes: SRIAA.NT
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 191.30864 5 38.261728 104.242 .0000
Within groups 57.25926 156 .367047
Total (corrected) 248.56790 161
0 missing value(s) have been excluded.
Multiple range analysis for SRIAA.SORE by SRIAA.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
6 27 2.5925926 X
5 27 2.6666667 XX
1 27 2.9259259 X
3 27 4.0370370 X
2 27 4.2962963 X
4 27 5.6296296 X
contrast difference limits
1 - 2 -1.37037 0.32578 *
1 - 3 -1.11111 0.32578 *
1 - 4 -2.70370 0.32578 *
1 - 5 0.25926 0.32578
1 - 6 0.33333 0.32578 *
2 - 3 0.25926 0.32578
2 - 4 -1.33333 0.32578 *
2 - 5 1.62963 0.32578 *
2 - 6 1.70370 0.32578 *
3 - 4 -1.59259 0.32578 *
3 - 5 1.37037 0.32578 *
3 - 6 1.44444 0.32578 *
4 - 5 2.96296 0.32578 *
4 - 6 3.03704 0.32578 *
5 - 6 0.07407 0.32578
* denotes a statistically significant difference.
DÀI RỄ TRÊN MOI TRUỜNG CÓ IAA
Analysis of variance
Source of variation Sum of Squares d.f. Mean square F-ratio Sig. level
Between groups 88.938272 5 17.787654 41.902 .0000
Within groups 66.222222 156 .424501
Total (corrected) 155.16049 161
0 missing value(s) have been excluded.
Multiple range analysis for DRIAA.DAIRE by DRIAA.NT
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
6 27 3.7777778 X
1 27 4.1851852 X
5 27 4.6666667 X
2 27 4.8148148 X
3 27 5.6666667 X
4 27 5.8518519 X
contrast difference +/- limits
1 - 2 -0.62963 0.35035 *
1 - 3 -1.48148 0.35035 *
1 - 4 -1.66667 0.35035 *
1 - 5 -0.48148 0.35035 *
1 - 6 0.40741 0.35035 *
2 - 3 -0.85185 0.35035 *
2 - 4 -1.03704 0.35035 *
2 - 5 0.14815 0.35035
2 - 6 1.03704 0.35035 *
3 - 4 -0.18519 0.35035
3 - 5 1.00000 0.35035 *
3 - 6 1.88889 0.35035 *
4 - 5 1.18519 0.35035 *
4 - 6 2.07407 0.35035 *
5 - 6 0.88889 0.35035 *
* denotes a statistically significant difference.
PHỤ LỤC 3
Khối lƣợng của cây Trƣờng xuân hoa trƣớc và sau khi đông khô
Hàm lượng nước trong mẫu cây tươi = (0,683 - 0,088)*100/0,683 =87,1%
0,6 g vật liệu tươi = 0,6*(100 – 87,1)/100 = 0,0774 (g) =77,4 (mg) chất khô
x (mg) alkaloid/ 0,6 (g) vật liệu tươi = x*100/ 77,4(mg) alkaloid/100 mg chất khô
PHỤ LỤC 4
1. Kết quả chất chuẩn catharanthine (20mg/l) khi phân tích bằng CE
Trọng lượng tươi
Trọng lượng khô
0,5287
0,0673
0,5202
0,1117
0,4794
0,0589
0,5394
0,0645
0,4653
0,0558
0,471
0,064
1,043
0,1259
0,8399
0,1006
0,7234
0,0937
0,5919
0,0797
0,8955
0,1154
1,10151
0,119
Trung bình
0,6832675
0,088041667
2. Kết quả chất chuẩn vindoline (20mg/l) khi phân tích bằng CE
3. Kết quả của mẫu Trƣờng xuân hoa khô khi phân tích bằng CE
4. Kết quả CE phân tích mẫu Trƣờng xuân hoa tƣơi
5. Kết quả CE phân tích mẫu Trƣờng xuân hoa in vitro
6. Kết quả CE phân tích mẫu Trƣờng xuân hoa in vivo
7. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 7 ngày tuổi
8. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 14 ngày tuổi
9. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 21 ngày tuổi
10. Sắc kí đồ CE phân tích mẫu Trƣờng xuân hoa 28 ngày tuổi