TEF1
KHOA
MSSV : 1053010978
2014
ThS.
.
N TT NGHIP
SVTH: PHXINH i
1
PH
1.1. 2
1.1.1. 2
1.1.2. 2
1.1.3. 3
1.1.4. 3
1.1.5. 4
1.2. 6
1.2.1. 6
1.2.2. 7
1.2.3. Tef1 7
1.3. 9
1.4. 9
1.4.1. 9
1.4.2. 10
1.5. 15
1.5.1. 15
1.5.2. 15
1.5.3. 15
1.5.4. 16
U 17
17
in sillico 17
18
N TT NGHIP
SVTH: PHXINH ii
2.3. 20
2.3.1. K 20
in sillco 21
21
22
2.3.5. PCR 23
24
24
24
2.3 25
2.3.10. A 25
2.3 25
2.3.12. 25
26
3.2
28
34
3.3.1. 34
3.3.2. LAST 35
Tef1 36
N TT NGHIP
SVTH: PHXINH iii
TEF1 37
3.5 41
48
48
55
55
55
55
56
N TT NGHIP
SVTH: PHXINH iv
23
Tef1 26
Tef1 34
37
-mercaptoethanol. 38
-mercaptoethanol. 39
43
- 45
Tef1
47
Tef1 47
Tef1
Genbank 48
Genbank 54
N TT NGHIP
SVTH: PHXINH v
Cordyceps militaris 2
5
5
8
Tef1 H. Jecorina 9
Tef1
29
Tef1
30
Tef1
31
35
Tef1 36
Chloroform 37
-mercaptoethanol 38
39
40
Tef1
40
41
- 42
-
42
- 43
- 43
- 44
44
N TT NGHIP
SVTH: PHXINH vi
- 44
- 45
- 45
Tef1 46
46
Tef1
49
Tef1
50
Tef1
51
N TT NGHIP
SVTH: PHXINH vii
ATP : Adenosine triphosphate
BLAST : Basic Local Alignment Search Tool
bp : base-pair
DNA : deoxyribonucleotide triphosphate
ML : maximum likelihood
MP : maximum parsimony
NJ : neighbor-joining
rDNA : ribosomal DNA
RNA : ribosomal RNA
TEF1 : The elongation factor 1 alpha
N TT NGHIP
SVTH: PHXINH 1
m
cao ,
N
c . n
c hay
(anamorph)
x Tef1 -1
cho
Tef1 au hay
trong .
Cordyceps
Cordyceps
gen Tef1
.
N TT NGHIP
PH 2
1.1.
1.1.1.
Hepialus armoricanus [40
Cordyceps sinensis
.
[38
].
HCordyceps militaris [44]
N TT NGHIP
PH 3
1.1.2.
Cordyceps -5000m ,
.
[39], 25
].
Cordyceps spp
[21]
[38].
1.1.3.
C. sinensis
[7]. [40].
1843, M.J. Berk
Sphaeria sinensis
C. sinensis [7].
1.1.4.
1.1.4.1.
Wang Be Jen
C. sinensis
2
[33].
e C. Ophioglossoides
[39].
1.1.4.2.
N TT NGHIP
PH 4
[41],
[42].
1.1.4.3.
Thitarodes armoricanus,
[13], [27].
[13
Theo McCoy (1990),
[23].
Beauveria bassiana, Metarhizium anisopliae
Normurea rileyi [3].
1.1.5.
1.1.5.1. Cordycepin
-
C. militaris
C. sinensis
[15
C. kyushuensis C. sinensis
militaris Cordyceps
[28
N TT NGHIP
PH 5
[37].
]
1.1.5.2. Polysaccharide
minh polysaccharide
e
[33], [18].
1.1.5.3. Ergosterol
C.
militaris C. sinensis
mg/gmg/g.
[39].
[44]
N TT NGHIP
PH 6
1.1.5.4. Nucleotide
Nucleotide
e
nucleotide e
. e trong Cordyceps.
Xie Jian
C. sinensis
[36].
1.1.5.5.
-33,0
acid aspartic, threonin, serin, glutamat, prolin, glycin,
cao
[14].
1.2.
1.2.1.
[30].
danh n].
N TT NGHIP
PH 7
[30], [32].
Isaria siclairii
Cordyceps sobolifera
Beauveria
sobolifera Cordyceps sobolifera [20
(anamorph)
(teleomorph)
1.2.2.
-
N TT NGHIP
PH 8
Tef1 1.2.3.
Gen Tef1 (translation elongation factor 1) -1 [25].
EF-
40S, [24].
-t
ribosome di
EF-1
[10].
[25]. Gen Tef1 c
[6] [19]. Buyck ng gen Tef1
(C. tenuithrix C. altipes) trong chi Cantharellus.
gen Tef1
Tef1
[8]. Tef1
.[25]
,
Tef1 m
1200bp [26].
[22].
N TT NGHIP
PH 9
1.5. Tef1 H. Jecorina [12]
1.3.
Cordyceps sinensis, Cordyceps militaris Cordyceps sabrolifera [5
Cordyceps nutans
Cordyceps gunnii [4].
Cordyceps militaris
(Isaria tenuipesCordyceps nutans) [4].
N TT NGHIP
PH 10
1.4.
1.4.1.
.
.
sinh sinh 1.4.2.
1.4.2.1.
N TT NGHIP
PH 11
nhau. gen hay
1.4.2.2.
D
1.4.2.3.
1.4.2.4.
.
N TT NGHIP
PH 12
u
Jukes-Cantor, Kimura 2-
parameter (K2P), Tamura 3-parameter, Hasegawa-Kishino-Yano (HKY), Tamura
Nei, General Time Reversible (GTR)
1.4.2.5.
-and-
-and-
-and-
t t
N TT NGHIP
PH 13
1.4.2.6. -
P-
ch
P
-
joining.
or-
-
-
N TT NGHIP
PH 14
lnL
1.4.2.7.
1.5.
1.5.1.
[35].
N TT NGHIP
PH 15
1.5.2.
( =300nm).
.
1.5.3.
(guanine), T
[1].
[1].
1.5.4.
khi