Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 4

Chapter 4 Characterization of CD38 Expressed in Mitochondia from Murine Brain
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Figure 4.12 CD38 immunoreactivity in the CD38 KO mouse cerebellum, labeled
with goat polyclonal CD38 antibody-sc-7049. No immunoreactivity was observed in
the granular layer. The insets (A & B) are enlargement of the encircled area of the
granular layer. M-Mossy fiber terminals.
Scale bar: 2µm. A & B, 1µm.
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Figure 4.13 CD38 immunoreactivity in the mouse cerebellum, labeled with goat
polyclonal CD38 antibody-sc-7049. Immunoreactivity was present in the perikarya in
association with the granular cell membrane viewed at low magnification. Upper
panel: intense immunoreactivity observed in the granule cells, identified by its
electron dense nuclei. Immunoreactivity was most intense with mitochondria.
Varying degree in CD38 immunostaing of mitochondria observed in different
compartment. Lower panel: immunopositive mitochondria located in Basket cell.
The enlargements of the encircled area of the basketcell are shown in the following
page. Blood vessel was heavily stained with CD38 antibody (arrow).
Scale bar: 1, 2µm.
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Chapter 4 Characterization of CD38 Expressed in Mitochondia from Murine Brain
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Figure 4.14 CD38 immunoreactivity in the mouse cerebellum, labeled with goat
polyclonal CD38 antibody-sc-7049. A, B: intense CD38 immunostaining labeled the
outer membrane of mitochondria (arrow), ER as well as plasmalemma (arrow head).
Note the immunostained ER and mitochondria were in close proximity with each

other (B). Immunopostive mitochondria in close proximity with plasma membrane (A)
Scale bar: A, B, 0.5µm.


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