Thu thập nguồn gene và tổ chức dữ liệu gene 9.pdf

Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
163
KVM-6 Reverse primer 5'-TGTCCAATCGTAAGCGTTCCT-3'
55 maize Bt11 IVS2-2
adh 1S-Intron2
(IVS2) and
phosphinothricin
N-acetyl
transferase (pat)
gene Forward primer 5'-CTGGGAGGCCAAGGTATCTAAT-3'
PAT-B Reverse primer 5'-GCTGCTGTAGCTGGCCTAATCT-3'
56 maize T25 T25-F7
phosphinothricin
N-acetyl
transferase (pat)
gene and CaMV
35S terminator Forward primer 5'-ATGGTGGATGGCATGATGTTG-3'
T25-R3 Reverse primer 5'-TGAGCGAAACCCTATAAGAACCC-3'
57 maize MON810 VW01
5' junction between
the host maize
genome
and the CaMV 35S
promoter of the
transgene Forward primer 5'-TCGAAGGACGAAGGACTCTAACG-3'
VW03 Reverse primer 5'-TCCATCTTTGGGACCACTGTCG-3'
58 IVR1-F
maize invertase
gene Forward primer 5'-CCGCTGTATCACAAGGGCTGGTACC-3'
IVR1-R Reverse primer 5'-GGAGCCCGTGTAGAGCATGACGATC-3'

Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
164
59 soya
Roundup
Ready TM RR-F Forward primer 5'-CCGGAAAGGCCAGAGGAT-3'
RR-R Reverse primer 5'-GGATTTCAGCATCAGTGGCTACA-3'
60 Soya lectin-F lectin gene Forward primer 5'-TGGTCGCGCCCTCTACTC-3'
Soya lectin-R Reverse primer 5'-GGCGAAGCTGGCAACG-3'
61 maize Event 176 CRY2-F
cryIA(b) synthetic
gene Forward primer 5'-CCCATCGACATCAGCCTGAGC-3'
CRY2-R Reverse primer 5'-CAGGAAGGCGTCCCACTGGC-3'
62 ZM1-F
maize high-
mobility-group
(hmg) gene Forward primer 5'-TTGGACTAGAAATCTCGTGCTGA-3'
ZM1-R Reverse primer 5'-GCTACATAGGGAGCCTTGTCCT-3'
63 Cat-f Forward primer 5'-ATAGCGACGGAGAGTTAG-3'
Cat-r Reverse primer 5'-TTTGTATTCTGAGCATAGTGA-3'
64 maize
Maize
StarLink
(CBH 351) Cry9C-5' Forward primer 5'-CCTATAGCTTCCCTTCTTCC-3' (?)
35Ster-3' Reverse primer 5'-TGCTGTAATAGGGCTGATGA-3' (?)
65 papaya
Papaya 55-1,
66-1 NosC-5'
Nos terminator and
CaMV 35S

promoter Forward primer 5'-TTACGGCGAGTTCTGTTAGG-3'
CaMN-3' Reverse primer 5'-CATGTGCCTGAGAAATAGGC-3'
Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
165
66 potato
Potato
NewLeaf
Plus
(RBMT21-
129,
RBMT21-
350,
RBMT22-
082) p-FMV02-5' Forward primer 5'-AAATAACGTGGAAAAGAGCTGTCCTGA-3'
PLRV01-3' Reverse primer 5'-AAAAGAGCGGCATATGCGGTAAATCTG-3'
67 potato
Potato
NewLeaf
Plus
(RBMT21-
129,
RBMT21-
350,
RBMT22-
082) PLRV-rep1-5' Forward primer 5'-CTTCTTTCACGGAGTTCCAG-3'
PLRV-rep1-3' Reverse primer 5'-TCGTCATTAAACTTGACGAC-3'
68 potato
Potato
NewLeaf Y

(RBMT15-
101,
SEMT15-02,
SEMT15- p-FMV05-5' Forward primer 5'-AAAAGAGCTGTCCTGACAGC-3'
Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
166
15)
PVY02-3' Reverse primer 5'-TCCTCCTGCATCAATTGTGT-3'
69 potato
Potato
NewLeaf Y
(RBMT15-
101,
SEMT15-02,
SEMT15-
15) PVY01-5' Forward primer 5'-GAATCAAGGCTATCACGTCC-3'
PVY01-3' Reverse primer 5'-CATCCGCACTGCCTCATACC-3'
70
MON 863
primer1 Forward primer 5'-GTAGGATCGGAAAGCTTGGTAC-3'

MON 863
primer2 Reverse primer 5'-TGTTACGGCCTAAATGCTGAACT-3'
71 adh1 primer 1 Forward primer 5'-CCAGCCTCATGGCCAAAG-3'
adh1 primer 2 Reverse primer 5'-CCTTCTTGGCGGCTTATCTG-3'
72 soybean lectin 1a Forward primer 5'-GACCTCCTCGGGAAAGTTAC-3'
GM04 Reverse primer 5'-GCCCATCTGCAAGCCTTTTTGTG-3'
73 XTPNT-S TNOS Forward primer 5'-caggtcgctgtcaTTGAATCCTGTTGCCGGTCTT-3'
XTPNT-A Reverse primer 5'-caggtcgctgtcaATAATTGCGGGACTCTAATC-3'

Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
167
74 XTPCM-S P35S Forward primer 5'-caggtggcagtcaTCATTGCGATAAAGGAAAGG-3'
XTPCM-A Reverse primer 5'-caggtggctgtgaCGAAGGATAGTGGGATTGTG-3'
75 XTPCP4-S EPSPS Forward primer 5'-caggctgctctgaCGAATATCCGATTCTCGCTGTC-3'
XTPCP4-A Reverse primer 5'-gacgacgcactcaCGCCCTCATCGCAATCCAC-3'
76 XTPGUS-S GUS Forward primer 5'-ccgtcactcgtcaCTGCTGTCGGCTTTAACCTC-3'
XTPGUS-A Reverse primer 5'-cagcaccaggtcaGCGTCGCAGAACATTACATT-3'
77 XTPNPT-S NPT-II Forward primer 5'-caggtcgctgtcaTTTCTCGGCAGGAGCAAGG-3'
XTPNPT-A Reverse primer 5'-caggtcgctgtcaACTGGGCACAACAGACAATC-3'
78 XTPNP-S PNOS Forward primer 5'-caggtcgctgtcaCAAAAGTCGCCTAAGGTCAC-3'
XTPNP-A Reverse primer 5'-caggtcgctgtcaTACCGAGGGGAATTTATGGA-3'
79 XTPCpTI-S CpTI Forward primer 5'-caggacgcacagaACCACCTCGGAAGTAATCAT-3'
XTPCpTI-A Reverse primer 5'-cagcacgcagtcaGGTTTGTAACAGAAATCAGCAA-3'
80 Spu-35S1-f CaMV promoter Forward primer 5'-CTTCGCAAGACCCTTCCTC-3'
Spu-cryVm1-r Cry V gene Reverse primer 5'-GCTGGAGAACGATTGGTGC-3'
81
EPSPS RR Soy-
specific Forward primer 5'-TGGCGCCCAAAGCTTGCATGGC-3'
Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
168

EPSPS RR Soy-
specific Reverse primer 5'-CCCCAAGTTCCTAAATCTTCAAGT-3'
82 Cry1Ab
Cry1A(b) (specific
for Bt Maize) Forward primer 5'-ACCATCAACAGCCGCTAGAACGACC-3'
Cry1As

Cry1A(b) (specific
for Bt Maize) Reverse primer 5'-TGGGGAACAGGCTCACGATGTCCAG-3'
83 TR03 18S rDNA Forward primer 5'-TCTGCCCTATCAACTTTCGATGGTA-3'
TR04 18S rDNA Reverse primer 5'-AATTTGCGCGCCTGCTGCCTTCCTT-3'
84 Zein3 zein Forward primer 5'-AGTGCGACCCATATTCCAG-3'
Zein4 zein Reverse primer 5'-GACATTGTGGCATCATCATTT-3'
85 PAT1 patatin Forward primer 5'-GTTATTATCCCTTAGCGTTGC-3'
PAT2 patatin reverse primer 5'-AATTGTTTTGTGAATGACGAG-3'
86 PAT3 patatin forward primer 5'-GGATCCAGCATTTTCTTCA-3'
PAT4 patatin Reverse primer 5'-TAGCTAACATCCATCGTAGAGG-3'
87 JSF3 Forward primer 5'-CACACAGGAGATTATTATAGGG-3'
JSR3 Reverse primer 5'-GGGAATAAGGGCGACACG-3'
88
ADH-F3
forward primer Forward primer 5‟-CgTCgTTTCCCATCTCTTCCTCC-3‟
Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
169

ADH-R4
reverse primer Reverse primer 5‟- CCACTCCgAgACCCTCAgTC-3‟
89
Bt113JFor
primer Forward primer 5‟-gCggAACCCCTATTTgTTTA-3‟

Bt113JRev
primer Reverse primer 5‟-TCCAAgAATCCCTCCATgAg-3‟
90 Ap1 AmpR(AF427133) Forward primer 5'-TATTCAACATTTCCGTGTCGC-3'
Ap2 Reverse primer 5'-TTTCGTTCATCCATAGTTGCC-3'
91 Nost5'F Forward primer 5‟-ttggcaataaagtttcttaagattgaat-3‟

Nost5'R Reverse primer 5'-acatgcttaacgtaattcaacagaaatt -3‟
92 RRS-F Forward primer 5‟-gccatgttgttaatttgtgccat-3‟
RRS-R Reverse primer 5‟-gaagttcatttcatttggagaggac-3‟
93 35S4-JUNC Forward primer 5'-ctttgaagacgtggttggaa-3'
RRS3-JUNC Reverse primer 5'-aaaggaaggtggctcctaca-3'
94 MaiY-F1 Forward primer 5'-TAGTCTTCGGCCAGAATGG-3'
MaiY-R3 Reverse primer 5'-CTTTGCCAAGATCAAGCG-3'
95 MaiJ-F2 Forward primer 5'-TTGGACTAGAAATCTCGTGCTGA-3'
Phần phụ lục
NGUYỄN KỲ TRUNG – LÊ THÀNH TRUNG
170
mhmg-rev Reverse primer 5'-GCTACATAGGGAGCCTTGTCCT-3'
96 GBSSI(GT1) Forward primer 5'-CAGCTATTTCCCAATTGGTTG-3'
GBSSI(GT1) Reverse primer 5'-GCGGTAGCATAAGTGCCAAG-3'
97 GBSSI(GT2) Forward primer 5'-GCAGGTTTGGATTACCAGGA-3'
GBSSI(GT2) Reverse primer 5'-CAGCAATGCCAGTTTTACGA-3'
98
MCRA013 and
CW14-11
mutant allent Forward primer 5'-AGAAATTTGAGAAGCAGATTGAGCAG-3'

MCRA013 and
CW14-11
mutant allent Reverse primer 5'-TCAATTTTGTCACATTCAACGAGC-3'
99
MC14-11
mutant allele Forward primer 5'-CTGACAAGGCAAGAGGAGTTGTT-3'

MC14-11
mutant allele Reverse primer 5'-ACAGCTGGTGCAGACTTTATGCTTA-3'

100 CaMV promoter Forward primer 5´-CCg ACA gTg gTC CCA Aag Atg gAC-3´
Reverse primer 5´-ATATagAggAagggTCTTgCgAAgg-3´
101
Roundup
Ready
Soybean Forward primer 5´-TCATTTCATTTggAgAggACACg-3´
Reverse primer 5´-ggAATTgggATTAagggTTTgTATC-3´