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O'IZZ-I
!
2010
^
BOYTli
BAO CAO
SAK
PHAM
NGIHtN ClJlJ »i TM CiP Bd
T«nd^t^i:
NGHlfiN
CCti
tJMG
DUNG
Qtl
TRINH
sAiN
XUIT
KIt4NG
HUYIT
TH4NH
sen,
B^
LifiT,
ROTA
SlJ
DIING
KJfiM
B.INH
vic
XIN

Citii
nhl^
S4
Ui:
PGS. TS.
U
TW
Luto
Cor
quan
tj6
chite)
cM
tri
dS
tM:
TruBg
tdm NgWto
«hi
Sia
xaa'f
Vfc
xin
vh
Sinh
ph&i
Y
t«f
Mas6Mm{aine&y.
Niton-2010

BOYTE
—BoCaca—
BAO CAO SAN
PHAM
NGHIEN CUtJ
DE TAI CAP BO
TSn
de
tai:
NGHIEN
ciru ifNG
DVNG
QUI
TRINH
SAN
XUAT KHANG HUYET THANH
Sdl,
BAI LIET, ROTA
SLT
DVNG
KIEM
DINH VAC XIN
Chu nhiem de tai: PGS.TS.LI Thi
LuSn
Off quan
(Th
cbvc) chii tri dl tai:
Trung tam Nghien
ciru
San xuat Vac xin va Sinh pham Y te

Ma
so
dl
tki (neu
co):
Nam
2010
BOYTE
sofi3ca
BAO CAO SAN PHAM NGHIEN
ciTu
DE
TAI
CAP BO
Ten de
tai:
NGHIEN
CCtJ ITNG DVNG
QUI TRINH SAN XUAT KHANG HUYET THANH
sen,
B^I
LIIPT,
ROTA
SU^
DyNG
KIEM DINH VAC XIN
Chii
nhifm
de tai:
Co*

quan chu tri de tai:
Cap quan
ly:
Ma
s^
dk
tki
(n^u
co):
Then
gian
thu-c
hi^n:
Tong kinh
phi thifc hi^n
de tai:
Trong
d6:
kinh
phi
SNKH :
Ngucin kh^c
(neu
c6):
PGS.TS.Le Thj Luan
Trung
tSm
Nghien
cihi
San xuat Vac xin va Sinh

pham Y te
BOYte
tir thang 7 nam 2007 den thang 12 nam 2009
910 trifu dong
598
tri^u
dong
tri^u
dong
Nam
2010
Bao cao
sdn
pham nghien
ciru
de tai cap
Bg
1
T£n
de tai:
NGHIEN
cut) iTNG DyNG
QUI TRINH SAN XUAT KHANG HUYET THANH
SOI,
BAI
LIET, ROTA
SU^
DUNG KIEM DINH VAC XIN
2.
Chu nhifm

dl
tai: PGS.TS.Le Thi Luan
3.
Co quan chu
tri
de tai:
Trung tam Nghien cuu San xudt
Vic
xin va Sinh
phAm Y
t^
4.
Cff
quan quan
ly
de tai: Bg Y
t^
5.
Thir ky detail
6. Pho chu
nhifm
de tai
ho|lc
ban chii
nhifm
de tai (neu co):
7. Danh sach nhirng ngiroi thirc
hifn
chinh:
Nguyen Dang Hien

Nguyen Thi Quy
Le Trung Dung
Nguyen
Thiiy
Huang
Nguyen Thi Mai Huong
Tran Bich Hanh
Dang Mai Dung
Nguyen Nu Anh Thu
Ngo Thu Huang
8.
C^c
de
t&i
nhanh (de muc) ciia de tai (neu co)
9. Thoi gian thyc hien de tai: tu thang 7 nam 2007
d6n
thang 12
nam
2009
Danh muc san pham
1.
Qui trinh cong nghf san xuat huyet thanh soi
2.
Qui trinh cong nghe san xuat huyet thanh bai lift
3.
Qui trinh cong nghf san xuat huyet thanh Rota
4.
Tieu chuan ca
stf

huyet thanh soi
5. Tieu chuan ca sa huyet thanh
b^i
lift
6. Tieu chuan ca
so*
huyet thanh Rota
7. Bai bao da dang
8. Dao
t^o
Thac sy
I. Qui trinh san xuat huyet thanh
sdi
Nguyen
vft
lieu
Khang nguyen Soi tit chung Edmonston do vien Kitasato cung cap;
Tho trong
lugng
ttr 1,8 ~ 2,5 kg;
Te bao Veto do vien Kitasato-Nhat ban cung cip;
Moi trucmg hoa
ch4t:
MEM,
NaHCOs
7,5%, BS, PBS (-), khang sinh P-S, Hank, chit
bao quan vacxin, ta dugc tiem dap ling mien dich (Adjuvant): Ta dugc hoan chinh
(CFA)
va ta dugc khong hoan chinh(IFA)
Vat lieu : Bom kim tiem trgn ta dugc, tu im CO2, pipet Aid, pipetman, diu tip cac loai,

may ly tam lanh, ly tam thucmg, chai chiia mau
(100
ml, 500 ml ),
phiSn
24
giSng.
Dung
cu lay mau tho,
Phuong pkdp
Sdn xuai khdng nguyen
Sdi
Virus Edmonston Vcro7 da giam dgc tren 1 s6 loai li bao Vien Kitasato, mgt trong
so do la te bao Vero 7 lan
tnigc
khi chuyen sang Viet Nam, virut nay
ti6p
tyc dugc ciy
truy6n
2 lin lien tiip tren tl bao Vero theo qui trinh san xuit khang nguyen cua vien
Kitasato
Nhtit
ban ggi la chung Edmonston Vero 7/P2.
Hdm luang
khdng
nguyen:
10''°CClD50/0,5ml
Tieu chuan tho sir
dung:
Khang
the soi

truac
khi
thii
nghiem <4 don vi; Khoe manh,
khong bj bat ky benh ngoai da nao; Can nang: 2,0kg den 2,5 kg; Tho dugc
each
ly tu 7-10
ngay
Qui
trinh
gay ddp
irng mien
dich
tren
tho
Phucmg phap vien Kitasato-Nhat ban (Khang nguyen khong tinh che)
Stt
1
2
3
4
Ngay
0
07
14
28
Ngay thuc hien
10-09-2008
17-09-2008
24-09-2008

08-10-2008
Lay mau5 ml
(Kiem tra
khang the)
Lay mau Mo
Lay mau Ml
(Lay mau giira
ky)
Cach tiem
Tiem(l ) 2,5 + 2,5 (ml)
(VRS+tadugc
FCA)
Tiem trong da nhieu vi
tri,
0,1
ml/1
vi
tri
Tiem (2) 5 (ml) VRS khong co
chat phu gia
Tiem vao duoi da dui 2 ben,
moi
ben
2,5
ml
Tiem
(3) 5 (mi) VRS khong co
chat phu gia
Tiem vao dual da
dilii

2 ben,
moi ben
2,5
ml
Tiem
(4)
10
(ml) VRS khong c6
chat phu gia
Tiem vao duoi da
diii
2 ben,
moi ben
5
ml
1.
Qui trinh san xuat huyet thanh soi
Nguyen vat
tifu
Khang nguyen Soi tir chung Edmonston do vien Kitasato cung cip;
Tho trong lugng tir 1,8 ~ 2,5 kg;
Te bao Vero do vien Kitasato-Nhat ban
cimg
cip;
Moi trucmg hoa
chit:
MEM,
NaHCOj
7,5%, BS, PBS (-), khang sinh P-S, Hank, chit
bao quan vacxin, ta dugc tiem dap ling

miin
dich (Adjuvant): Ta dugc hoan chinh
(CFA) va ta dugc khong hoan
chlnh(IFA)
Vat lieu : Bom kim tiem trgn ta dugc, tu im CO2, pipet Aid, pipetman, diu tip cac loai,
m4y
ly tam lanh, ly tam thucmg, chai chira mlu
(100
ml, 500 ml ), phiin 24
gilng.
Dung
cu
lay mau tho,
Phutfttgphdp
Sdn
xudt
khdng nguyen
Sdi
Virus Edmonston Vero7 da giam dgc tren 1 so loai tl bao Vien Kitasato, mot trong
so do
\k
te bao Vero 7 lin
tmoc
khi chuyin sang Viet Nam, virut nay tilp tuc dugc ciy
truyen 2 lan lien tilp tren tl bao Vero
dieo
qui trinh san xuit khang nguyen cua vien
Kitasato Nhat ban ggi la chung Edmonston Vero 7/P2.
Hdm lugng
khdng

nguyen:
10'°CClD50/0,5ml
Tieu chuin tho
sir
dung:
Khang
thi
soi truoc khi thu nghiem <4 don vi; Khoe manh,
khong bi bit ky benh ngoai da nao; Can nang: 2,0kg
din
2,5 kg; Tho dugc
each
ly tir 7-10
ngay
Qui
trinh
gay ddp
ting mien
dich tren tho
Phuong phap vien Kitasato-Nhat ban (Khang nguyen khong tinh
chi)
Stt
1
2
3
4
Ngay
0
07
14

28
Ngay thirc hien
10-09-2008
17-09-2008
24 - 09 - 2008
08-10-2008
Lay rnau5 ml
(Kiem
tia
khang the)
Lay mau
Mo
Lay mau Ml
(Lay mau giira
ky)
Cach tiem
Tiem
(1
) 2,5 + 2,5 (ml)
(VRS+tadugc
FCA)
Tiem trong da nhieu vj tri, 0,1
ml/1
vi tri
Tiem (2) 5 (ml) VRS khong co
chat phu gia
Tiem vao
ducyi
da
diii

2 ben,
m6i ben
2,5
ml
Tiem (3) 5 (ml) VRS khong co
chat phu gia
Tiem vao duoi da
diii
2 ben,
moi ben
2,5
ml
Tiem (4)
10 (ml)
VRS khong co
chat phu gia
Tiem vao duoi da ddi 2 ben,
moi
ben 5
ml
5
6
42
49
22-10-2008
29 - 10 - 2008
Lay mau M2
(Lay het mau)
Tiem (5)
10

(ml) VRS khong co
chat phu gia
Tiem vao
tinh
mach tai
Gay mien dich
miii
1, tiem 2,5 ml khang nguyen + 2,5 ml ta dugc (ta dugc hoan
toan),
tiem trong da vimg lung. Gay
miin
djch miii 2 sau tiem miii 1 la 7 ngay, tiem 5 ml
khang nguyen, tiem duoi da
diii.
Gay
miin
djch
mOi
3 sau tiem miii 2 la 7 ngay, tiem 5 ml
khang nguyen, tiem duoi da
diii.
Gay mien dich mui 4 sau tiem mui 3 la 14 ngay, tiem
10
ml khang nguyen, tiem dual da
diii.
Gay mien dich mui 5 sau tiem mui 4 la 14 ngay, tiem
10
ml khang nguyen, tiem
tinh
mach tai.

Phuong phap cua CDC-Hoa ky (Khang nguyen tinh sach qua
duone)
Stt
1
2
3
4
5
6
7
Ngay
0
14
21
28
35
49
Ngay thuc hien
10-09-2008
24 - 09 - 2008
01-10-2008
08-10-2008
15-10-2008
29-10-2008
Lay mau5 ml
tinh
mach tai
(Kiem tra khang
thi)
Lay mau Mo

Lay mau Ml
Lay
mau M3
(Lay het mau)
Tiem
Tiem
(1
) MV + CFA
(1ml
/ con , tiem trong da lung)
Tiem (2 ) MV +
IFA
(1ml
/ con , tiem trong da lung)
Tiem (3 ) MV + IFA
(1ml
/
con,
tiem trong da lung)
Liy mau kiem tra huyet thanh sgi 3 lan, lan
1
truoc khi gay mien dich mui
1;
Lan 2
lay mau tai truoc khi gay
miin
dich miii 4 de kiem tra kha nang tao huyet thanh
giita
ky;
Lan 3 liy mau toan bg sau 7 ngay gay mien dich mui 5 (ngay

thii
49 ke tir ngay gay mien
djch
miii 1)
Tai ngay thir 49 liy toan bg mau tho vao chai det, bao quan
4°C
qua dem , tach huyet
thanh, ly tam 3000 vong / phut trong 20
phiit,
lay huyit thanh, sau do bao quan -
SO'C
cho
toi khi sir dung.
Xac dinh hieu gia huyit thanh bang phucmg phap trung hoa tren tl bao vero mgt lop.
Huyit thanh dugc bit hoat
56°C
trong 30 phut, sau do dugc pha loang bac 2 va dugc trung
hoa vol
100
dcm vj khang nguyen soi,
hin
dich trung hoa dugc gay nhiim tren te bao vero
1 lop, sau 2 tuin dgc
kit
qua. Hieu gia khang
thi
la nlng do huyit thanh pha loang nhit ma
tji
d6
tjo

ra phan img trung hoa (tl bao binh thutmg khong bi huy hoai)
2.
Qui trinh san xuat huyet thanh bai liet
Nguyen
li(u va
dung cu.
Khang nguyen Bai
liet
typ I, II, III
Ta dugc (Freunds complete adjuvant - FCA, Freunds incomplete adjuvant
-FIA)
Th6:Khang
thi
soi targe khi thir nghiem <4 dcm vi; Khoe manh, khong bi bit ky benh
ngoai da nao;Can
nSng:
2,0kg
din
2,5 kg; Tho dugc
each
ly tir 7-10 ngay
Te bao
Hep2C
cua to chiic
Y
te the giai
Xi lanh diing trgn khang nguyen va ta dugc
Ta dugc hoan chinh (CFA) va ta dugc khong hoan
chinh(IFA)
- Moi trucmg MEM

10%,
2% FBS
- Trypsin Versen va mgt
s6
dung djch khac
May
ly tam
Phi&n
nhua day det 96 gilng
Tu im 37°C va
36°C
- Pipet aid va pipetman cac loai
- Hood vo trimg va cac dyng cu khac phyc vu cho nghien cim
Phifffngphdp nghien
cicu.
Sdn xuit khdng nguyen bai
lift:
Nhan virut bai liet typ I, hoac typ II hoic
typIII
tren te bao vero mgt lop
vciri
nong do 1
CCIDjo
trong 300 tl bao vcri moi truang gay
nhiim DMEM tai nhiet dg
37°C
trong 72-96 gia, gat
hin
djch virut khi huy hoai tl bao
>90%.

Tinh sifch
khdng nguyen: Hon dich virut sau khi dugc dong tan 3 lan, ly tam 3000
vdng/phut
trong 30 phut, lay nuac noi. Can dugc sieu am va ly tam lay nuac noi. Nuoc
noi hgn lgi va dugc
Igc
voi co
Igc
lan lugt 0,65 (tm, 0,45
pm,
0,2
pm.
Hon djch virut
dugc sieu
Igc
vgi co dac
100-200
lin va dugc sieu li tiim 36.000
v6ng/phut/3 gio
tai
4'^C,
lay can, hoa loang can bang BMS va bao quan tai
-70''C
cho den khi sir dung
-
Cdch
gdy
ddp
ling
mien

djch tgo
khdng
the
bai li^t
Khdng
nguySn:
virut sabin typ
1
hoac typ 2 hoac typ 3
Him
liryng khdng
nguyen:
10'°CCID 50/0,1ml
Tho sir
dyng:
tieu chuin nhu tho
sii
dung cho san xuat huyet thanh scri
Cich gay dip
irng
mien djch: Phucmg phap CDC-Hoa Ky
Stt
1
2
3
4
5
6
7
Ngay

0
14
28
42
56
70
84
Lay mau
Lay mau5 ml
tinh
mach tai (Kiem tra
khang the)
Lay mau Mo
LiylnauMl
Lay mau
M2
Lay mau M3
Lay
mau M4
Lay
mau M5
Lay
mau M6
(Lay het mau)
Cach tiem
Tiem
(1)
0,2 + 0,2 (ml)
(VRRT+la
dugc FCA)

Tiem trong da nhieu
vi
tri, 0,1 ml/1 vi tri
Tiem (2)
1
+
1
(ml)
(VRRT+ta
dugc FIA)
_
Tiem vao co
diii
2 ben, moi ben 2 vj tri, moi vi ta'
0,5
ml
Tiem (3)
1
+
1
(ml) (VRRT+ta dugc
FL\)
Tiem vao co
diii
2 ben,
mil
ben 2 vi tri,
mli
vi tri
0.5 ml

Tiem (4)
1
+
1
(ml) (VRRT+ta dugc FIA)
Tiem vao ca
diii
2 ben, moi ben 2 vi tri, moi vi ta'
0,5
ml
Tiem (5)
1
+
1
(ml) (VRRT+ta dugc FLA)
Tiem vao co
diii
2 ben, mli ben 2 vi tri, moi vi ta'
0,5 ml
Tiem (6)
1
+
1
(ml) (VRRT+ta dugc FIA)
Tiem vao co
diii
2 ben, moi ben 2 vi ta', moi vi ta'
0,5 ml
—___^
Thu huyet thanh

: ly tam 3000
vong/phiit.l 0
phut. Thu huyit thanh bao quan
6
-20°C.
Kiem tra
hi^u
gid:
khang huyet thanh bang phucmg phap trung hoa vi lugng tren tl bao
Hep2C
3.
Qui trinh san xuat huyet thanh rota
Nguyen
vat
U^u
Khang nguyen
rota
typ G1P8,
G4P6,
RRV
T^
bao than tho Rherus
MA
104
Moi trucmg DMEM, 199
Tho tieu chuin
Ta
dugc
hoan chinh (CFA) va ta dugc khong hoan chinh(IFA)
Trypsin Versen va mgt

s6
dung dich khac
May
ly tam
- Phien nhua day det 96
giSng
-
Tu^37''C va36''C
Pipet aid va pipetman cac loai
Hood vo trung va cac dung cu khac phuc vu cho nghien curu
Phuffng
phap
Phifffngphdp sdn xudt khdng nguyen
Te bao
MAI 04
mgt lap hoac te bao vero mgt lap,
d?p
dugc su dyng cho su nhan len
cua virut rota. Te bao dugc rua 2 lan vcri moi truang DMEM im, sau do dugc dat tai
2il^C
it
nhat
3
gia.
Virut dugc hoat hoa vai trypsin nong do
15^1/ml
tai nhiet dp
37°C
trong
1

gia, sau
do hap phu virut len te bao tai nhiet do
37*'C
trong
1
gia
Bo het moi
truong
gay nhiim, nia te bao
01
lan bang moi
iruong
DMEM am khong
CO
trypsin, sau do cho them moi truang co trypsin vai nong do
10-20[ig/ml.
Dat chai te bao
da gay nhiim virut tai nhiet do
37''C
trong
3
ngay.
Gat
h6n
dich virut, dong tan
3
ISn,
ly
tam 3.000 v6ng/phut/30
phiit

bo can.
Nuac
n6i
thu dugc sieu ly tam 25.000 v6ng/phut/2 gia tai
4'*C
voi
n6ng
do ducmg
40%.
Bo nuac
n6i,
thu can va hoa can voi TNC, sau do pha loang khang nguyen thu dugc
sau cho lugng virut khoang
10^-10^
ffii/ml
Phuvng phip gay dap
irng
mien dich tao khang the rota
{CDC-Hoa
Ky)
Stt Ngay Lay mau
Cach tiem
Lay mau5 ml
tinh
mach tai (Kiem tra
khang the)
Lay
mau Mo
Tiem
(1

) 0,2 + 0,2 (ml) (VRRT+ta dugc FCA)
Tiem trone da nhiiu vi tri. 0.1 ml/1 vi tri
2
3
4
5
6
7
14
28
42
56
70
84
LayTnauJvll
Lay mau M2
Lay mau M3
Lay mau M4
Lay mau M5
Lay mau M6
(Lay het mau)
Tiem (2)
1
+
1
(ml) (VRRT+ta dugc FIA)
_
Tiem vao ca dui 2 ben, mli ben 2 vi tri, mli vi tri 0,5
ml
Tiem (3)

1
+
1
(ml) (VRRT+ta dugc FIA)
_
Tiem vao
eg
did 2 ben, moi ben
2
vi tri, mli vi tri 0,5
ml
Tiem (4)
1
+
1
(ml) (VRRT+ta dugc FIA)
_
Tiem vao co did 2 ben, mli ben
2
vi tri, mli vi tri 0,5
ml
Tiem (5)1 +
1
(ml) (VRRT+ta dugc FIA)
_
Tiem vao ca dui 2 ben. moi ben 2 vj tri, mli vj tri 0,5
ml
Tiem (6)
1
+

1
(ml) (VRRT+ta dugc
FIA) _
Tiem vao co
diii
2 ben. moi ben 2 vi tri, mli vi tri 0,5
ml
—____^
Thu huyet
tiianh:
ly tam 3000 v6ng/phut,10 phut. Thu huyet thanh bao quan a < -
20 C cho toi khi sii dung.
Kiem
tra
hi^u
gid: khang the trung hoa bang phucmg phap ELIZA
4.
Tieu chuan khang huyet thanh Soi
Tieu chi
dinh
gia
Vo trimg
Hi$u
gia khang
huydt
thanh
Tinh dac hieu
Tieu chuan Nhat Ban
Vo trimg
>4.000

Dac hieu bang thu nghiem
nhan dang qua ky thuat
miin
dich huynh quang
Tieu
chuin cff sir
Vo trimg
>4.000 cho thu nghiem xac dinh tac
nhan
ngo^
lai
>
1.000 cho thu nghiem nhan dang
virut Soi
Dac hieu
bSng
thu nghiem nhan dang
qua ky thuat mien dich huynh quang
5. Tieu chuan khang huyet thanh Bai liet
Tieu chi danh gii
Vo trimg
Hi?u
gia
khang
huyet
thanh
bai
liet
Tinh
dac

hi^u
khang
huyet
thanh
Typ I
Typ
II
Typ III
Typ I
Typ II
Typ III
Tieu chuin Nhat Ban
Vo trimg
>16.000
>
16.000
>
16.000
Cheo typ 2,
3
: <32
Cheo typ 2, 3 : <32
Cheo typ 2, 3 : <32
Tieu chuan
ca
so*
Vo trimg
>65.000 cho thit
nghi?m
xac dinh tac

nhan ngoai lai
<65.000 cho thir
nghi?m
xac dinh
hieu gia vac xin thanh pham
>8.000 cho thir nghiem xac dinh tac
nhan ngoai lai
<8.000 cho thu nghiem xac dinh
hieu gia vac xin thanh pham
>16.000 cho thir nghiem xic dinh tac
nhan ngoai lai
<16.000 cho thir nghiem xac dinh
hieu gia vac xin thanh phim
Cheo typ 2,
3
: <32
Cheo voi typ 1,3:
<16
Cheo vai typ
1
,2 : <32
6. Tieu chuin khang huyet thanh Rota
Tieu chi dinh gii
Vo
trimg
Hi?u
|G1P8
Tieu chuan Hoa Ky
Vo trimg
Tieu chuan ca

sa
Vo trimg
>
6.400
gia
khang
huyit
thanh
Tinh dac
hieu khang
huyet
thanh
Wa
DSl
RRV
RRVmix
G1P8
Wa
DSl
RRV
RRVmix
5.000
5.000
Dac hieu bang thir nghiem
nhan dang qua ky thuat
miin
dich huynh quang
> 1.000.000
>100.000
> 1.000.000

> 100.000
Dac hieu bang thu nghiem nhan
dang qua ky thuat mien dich
huynh quang
7. Bai bao da dang
Bit bao da dang:
01
bai
Nghien cim san xult huyit thanh Soi sir dung cho
kiim
dinh vic xin. Tap Chi Y hgc
du
phong, 2008, Tap XVIII
si
6(98),
tr.74-78
8. Dao tao Thac sy
Bko
t^o:
01 thac
sS'
Ten dl
tai:
Nghien
ciiu
san xult khang huyet thanh Bai liet tren tho va danh gia tinh an
toan cua vac xin Bai liet trong phong thi nghiem.
Chuyen nhanh: Thii
y
Ma

si
60.62.50
Ba bao ve vol diem xult
sac
nam 2008
Bp GIAO
DIIC
VA DAO TAO
TRUOfNG
DAI HOC NONG NGHIEP HA NOI
ca
NGUYEN THI NGA
NGHIEN
Ciru
SAN
XUAx
KHANG THE BAI LIET TREN THO
VA DANH GIA TINH
AN
TOAN
CUA VAc XIN BAI LIET
TRONG PHONG THI NGHIEM
LUAN VAN THAC
SI
NONG NGHIEP
Chuyen nganh : THU V
Mas3
: 60.62.50
Nguoi huong dan khoa hoc: PGS.TS LE THI LUAN
HA

NOI
- 2008
^
—
irt]
MUC
LUC
1
Md DAU
1
1 -' •
Tinh cap
thidt
ctia
d8
tai 1
1
-2.
Miic
dich cua de
tai:
3
2.
TONG QUAN TAI LIEU
i
2.1.
Benh bai liet do virus
i
2.2.
Lich

sir
phan bo 5
2.3.
0 Viet
Nam
5
2.4. Dac diem virus bai liet 5
2.5.
Benli
bai liet a ngiroi
13
2.6.
Tieu chuan Viet Nam ve
phuonng
phap kiem tra tinh an toan
ctia
vacxin phong bai liet
16
2.7.
Vacxin phong benh bai liet
18
2.8.
Nghien cuu san xuat khang the bai liet 21
3.
DOI TUONG,
DIA
DIEM,
NOl
DUNG VA PHUONG PHAP
NGHIEN

Ciru
23
3.1.
Doi
tii'cmg
nghien
cmi
23
3.2.
Dia
diem 23
3.3.
Noi dung ngliien
ciiu
23
3.4.
Plutong
phap nghien
ciru
24
4.
KET QUA -
BAN
LUAN 50
4.1.
Ket qua nghien ciru san xuat khang the bai Met 50
4.1.1.
KSt
qua san xuat khang nguyen bai liet 50
4.1.2.

Ket qua san
xuk
khang the tir huyet thanh Tho
\kn
thir nhat
51
4.1.3.
Kit
qua san xuat khang
thS
tir huyet thanh Tho lan thir hai 57
4 2.
Kit
qua kiem tra tinh an toan vacxin bai liet trong phong thi
nghiem 63
^•2.1.
Kiem tra nguyen lieu dau cho san xuat vacxin bai Het - khi san
xuat 63
Ket qua kiem tra te bao sir dung cho san xuat vacxin bai
Met
trong
bay nam tit nam 2000 - 2006 65
4
2.3.
Kit
qua kiem tra an toan vacxin ban thanh pham
4-3.
Ban luan
4.3.1.
Nguyen heu

dSu kill
4.3.2.
Chat hrgng te bao
4.3.3.
Hieu gia
kliang
huyet thanli
5.
KET LUAN - KIEN NGHI
5.1.
Ket
luan
5.2.
Kien
nghi
TAI
LIEU THAM
KHAO
70
77
77
79
81
84
84
85
87