Chapter 3 dac hieu enzyme e

Enzyme specificity
• Absolute
• Linkage: digestive enzyme
• Group: carboxypeptidase
• Stereo: malatdehydratase


Absolute specificity
• Urease

• Arginase
• Renin
Application


Linkage
• Lipase
• Protease
• Amilase (α- , β- , -)
Application


Group
• Aminpeptidase
• Carboxypeptidase
• NADH dehydrogenase


stereospecific

subclass
3. Hydrolase

– 3.1. Esterase
–
–
–
–

• 3.1.1. Ester carbocylic

3.2.
3.3.
3.4.
3.5.

Glucosidase
Etherase
Peptidase
Amidase

Enzym code: 4 digits
Lipase (EC 3.1.1.3)
Trypsin (EC 3.4.21.4)
Alcoholdehydrogenase (EC 1.1.1.1)
Aldolase (EC 4.1.2.13)
Catalase (EC 1.11.1.6)

Starch hydrolyzing enzyme
• α- amilase: most thermal stable,
• β- amilase: acid stable
Temp max: 700C
• - amilase: working pH: 3,5-5,5


peptide hydrolase
• Endo-peptidase (proteinase): pepsin, trypsin,
chimotrypsin
– Serin Protease
– Thiol Protease
– Acid Protease (aspartic proteinase)
– Metalo Protease

• Exo-peptidase (peptidase)
– Amino peptidase
– Cacboxyl peptidase



Application of hydrolysing enzyme
• amilase (-,-,-amilase)
• protease (animal, plant,
microorganism)
• Lipase
• Pectinase
• Cellulase
• Others

Oxydoreductase
•
•
•
•

Dehydrogenase
Polyphenoloxidase
Catalase
Peroxidase