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41
PHỤ LỤC

HÓA CHẤT CHO ĐIỆN DI
 TBE (0,5X)
Tris HCl 3,94 mg
Acid Boric 1,39 mg
Na
2
EDTA 93,06 mg
Nước cất vừa đủ 500 ml
pH = 8,3
 Agarose (1,65%)
 Loading dye
Bromophenol blue 0,25%
Sucrose 40%
TE 1X vừa đủ 100%

HÓA CHẤT CHO NHUỘM GEL
TBE 1X
Ethidium bromide 10 mg/ml

MÔI TRƯỜNG VÀ THUỐC THỬ
 Môi trường
Nước pepton đệm
Pepton 10 g

Sodium chloride 5 g
Disodium hydrogen phosphate 9 g
Potassium dihydrogen phosphate 1,5 g
Nước cất vừa đủ 1000 ml

NA
Pepton 5 g
Sodium chloride 5 g

42
Beef Extract 1,5 g
Yeast Extract 1,5 g
Agar 15 g
Nước cất vừa đủ 1000 ml
pH = 7,4  0,2 (25
0
C)

MAC
Bacto pepton 17 g
Bacto Proteose pepton 3 g
Bacto Lactose 10 g
Bacto Bile salts 1,5 g
Sodium Chloride 5 g
Bacto agar 13,5 g
Neutral Red 0,03 g
Bacto Crystal Violet 0,001 g

SMAC
Pepton 20 g

Sodium chloride 5 g
Bile salt 1,5 g
Sorbitol 10 g
Crystal violet 0,001 g
Neutral red 0,003 g
Agar 15 g
Nước cất vừa đủ 1000 ml

SIMMON CITRATE
Na citrate 2 g
NaCl 5 g
K
2
HPO
4
1 g
NH
4
H
2
PO
4
1 g
MgSO
4
0,2 g

43
Bromothymol blue 0,08 g
Agar 15 g

Nước cất vừa đủ 1000 ml

MR – VP
Pepton 7 g
Glucose 5 g
K
2
HPO
4
5 g
Nước cất vừa đủ 800 ml
pH = 6,9  0,2
 Thuốc thử
VP (Voges Proskauer)
Dung dịch A:
Alpha napthol 5 g
Ethanol tuyệt đối 100 ml
Dung dịch B:
Potassium hydroxyde 40 g
Nước cất 100 ml
MR (Methyl red)
Methyl red 0,1 g
Alcohol 300 ml
Nước cất vừa đủ 500 ml


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