ỨNG DỤNG CÔNG NGHỆ VI SINH TRONG SẢN XUẤT THUỐC TRỪ SÂU SINH HỌC ( BACILLUS THURINGIENSIS )



 
!"

#$$%
&'(')$'$*BACILLUS
THURINGIENSIS+
,-$./0
1,
.2-345-67-859:;<=;:>
 
 
 !
"# 
$%&' ()
*#&+ )

?@'
+,-./012345% * 6 078+9 43"4 47:4
,8;0* "0' <=#>08/?+=# @3-?7#A0BC/0+
=*,8;0D6 ,EFG "0'=#0H 4A DI,0% "0'BJ
?C4 K=#>00L%"=/0"020*A+ ,/0
"0'B
 >   #L @8,"*4F/#7 ?0%?6F/
,M0N=O P @4?C=82 ,-./007 CQ B7 CQ 5-82
0M 8;0=L /0?>  C'7 CQ 4 C06 FG3R0S 6 =*F& *
"0'5'#,"*452*4 TF*42*U/0?> = /0D."#;3
8 V0W,8;08#>0=OT < C0W36PL H  V5-0
058 V0W4FX?=# 0,8;0*B
7 5-820M 8;0CQ  < C+?W#Y0>Z07  C2[-

0% V0W,8;0*4 C2[-06 FG03*#8>48+=*
F& *0% \#>0=* "0'B%4? \8L?W#Y0>?C4
07 0S,"CQ  < C+O ?W#4"L06 *D1T #1
0@8,H DT] @B,EFG07 +=8'?> ,^=# 
,"*@07 40*%0_D607 `[0W,"*,802 Q=Q B
JD-0%F20," 4=* C3Y06 FG>407 0S,"CQ =#
-#,K0a200_?F* P 6 [0W C_ 4#>0#A00%F20 6 ,8;0 C_ 4
#A0D6 =##/0 "5b,06BcdFX?;[-=#./02L=*F& 
#eF[0W5??>05 7 &807 f8=#06360F& *
e[0W,"*3600WD?dD607 8D15&D7 5`0%?& 4
?'0@_0 C *00 _[0WfBg1 h8;407 0S,"Q  C0@
0'0*="F0#10@%?d"1#?#10@?/04 8
D1D_B[0QV -4?C=D,EFG=%0G 407 0S,",^0_ =<
0?/04?8 i0H 8D?*0?'?>,Q ,^"* ,H DT]
@40;# _+0@O3FX?0E8B
_8jL;[-0%%?d?A0#>0 5H 008+82 3600W
=*07 5-820M 8;0#4?-#5-3- C06 FGF20," 42=M 
C43Y06 FG>4 C2[-?78+=*,"4D1-`? /0
=O1,-4D1-`??/00'0Q0 <#10@D1D_4
D10*%0_D607  P,"*4D1=##/0?L,8;0 C_ 4
L=0%?& 8L8,8;0 C=O  @4=* C2[-DkFB
cC= 6  3l#07 5-820M 8;08,8;0B
20#=#>0 1238V!)mF",7,7`10180%
02 06 420*1238X=,-./0 _4 #8&0_[
0Q0+D0[7 F"BnA F\?+D2D_;4?/0?4,1R0;02
3600W1234 < _8jD_;C4l#=*0*?+D20;
=O ,M3/00W P 6 =,"524"0Y0/0#D-o(m#\
#BVL80C[] P@F"20#0@=*#FG07 5-82
0M 8;04/0=82 ,EFGD1?K[?&8+0@8=+=O40;# _
F\ - 6 07 5& /#%?d?W=*+;[-?60 BJ?C 12,-./0

6  3l#07 5-820M 8;08,8;0??O [0"#83600W
#>03Y58#*#^Vb#-[0 6 8/?+8+#10@4 @4 "
5b2,06BjL=IF0%%01?d Q?+0Z912,-./0 
3l#07 0S,",Q :?W=#0W=;B
/ AB' '
\8,M?F* P "0'0j,M?F* P,"*`20# </0
=B#4020*F,"*D-()om0;# _ CD=%?o)mB
3,"*D-!)=0> Q85>BcWDN 3G 0j0*4
#i# 03-;3()B0/07 5-820M 8;0B#(` 0 C?
=*C /08(0%0V3l#D6 40&6D-!02pqJBJ
,"* CD-D607 %@0'0Q00@0'?>,EFGFX
?F=O07 0S,"0,-3l#1230 "#/00 
@,EFG4-`5/0=O?78#10@4,H DT] >?'8 _@
0'0Q0B4 6 ,-3l#D10W./0Dl?O %-`=?
0;3 P1F"Bc" <=#>006 0H = 1F"20#D
;3rsB_8j8;#,M3600W P,-./007 5-820M 8"08
,8;0 #V0%0 <` 0B
:.:.CD-EFGHEIJ-877K4L5DMN59-O5-EP5QRS77-RTD7KUEVRE45--WD
%0482 ,EFG8,8;005-820M 8;0 ?O 360
0W#*#^4?d0j#?O + P8,8;0 CD-"52  10\B
P8,8;0?0%?O 360282?,EFG?WF20,"*8 1
0\=8Dlt ==,0],,et0fFu,v00j#0/?0%41?A00%=
t ==,,00#Bc#4=8Dl?O w,0t]=]3602
8?A00%=t ==,0],,D1% H06 ""520%=,"
.6#`0h4cH Bc#4=?0%t ==,0],,?O ,E
FG=#07 0S,"0*638?L0;3%#)4t0?d?O ,EFG
>d0*nxBt0?d?O % H8?O HFG>d0%+[7 8
?]#=*2[-?6DW`Zgy4$%z1<4634cH 4{7 4;0
t-U| 040S 70;3D}?0;3D}#>0,7 V[% HDQ
?d5N0?,-./0 6  3l#F20,"0St0B2=M  P 6  3l#t0,-./0`

)
0 ?78,"0V4,".4," 7=6=*T0VH?*0Zm~m4
(m~(m4(m~mB
2 HFG 6  P8,8;0 CD-"?> 8 10\8,"*
8 106 5-820M 8;00%08 0?d8?3600W 
#*#^F00  6 =*07 5-820M 8;0CQ ?"1##1
0@8"?>  ?>8;08 @B
:.=.X59YRN5GH7-RTD7KUEVRE45--WD.
:.=.:.Z-8454[\GH7-RTD7KUEVRE45--]D.
7 0S,"8,=L 3l#,Q ?O ,-./00S 6  P8
,8;0?O 1 /0%#10@FF•D6 0]3V3630P
14560P 1A 3V363=%#] 123?W0*L 03l# C
/0=O  CD-3R0S?O  6 =*,"* "0'14="#23B
:.=.=.-O5-J-^5DMN7-RTD7KUEVRE45--WD.
7 0S,",Q ?O ,-./08HFGFM8D-"524?>
 V0W 10\4,"*B03 _ P07 5-820M 8;08,8;0=
6  P8,8;0 CD-"52  10\4,"*A  6 F*50E
P KB
:.>_D`4L\DMN7-RTD7KUEVRE45--WD.
:.>.:_D`4L\GODaD-b78Dcd59DMN7-RTD7KUEVRE45--WD
eR`4L\
− q807 5-820M 8;0CQ 0j07 0S,",Q  C+?A
?W#070V?C=Z
− g1"?> * @4?>8;04 "0'4 CD-0%F20#>0
6  Q=Q  6 =*,"52B@0C K C0_?A 2 0
82 0%F20 10\BD?C407 0S,"CQ "?> *?78
@4,K 403.K ="F#>0,7 R=06 ""0BJ

D1"?> *?78 @4=*D1-`./?78,M360
0W PD28,8;0[2 - "0'4% 3l#07 5-

820M 8;08,8;0D1368• "5b2,064D1"1#
#10@,7B
− 2 ,EFG 6  3l#07 0S,",Q 5-820M 8;0 ?
36020/20O=@07 ` 6 = 10\BcC=?+/0
?6[0"#48j` 6 =*07 6Q 0S,"52F?d?O ,EFG="
F0S0 ?882 ,EFG R[60y02%20_=@
07 ./02` 6 =,"5245N05> @03-"F'?>,E
FG=%#2[-=* H-#FB
− C0W W] 3782 0*0_?> ?78 10\"*, 
+=* "0'0*% "0'0MC CD-D6?O  6
=,"*Bc"=06 FG#L 3l#07 0S,"6Q D1
0W C?O B
− 6  3l#5-820M 8;08,8;0D6 8 6 O3 /06Q 0S,"
*5`5- /0,7 PC40H C H 6 "07"524=# 0 1
0\=L8,8;0,7BJ8;82 5-[-A .E=I 6  3l#
?'> < CL% D6 ,8 6 O3 /06Q
0S,"*Bn>020O0M%0*0;=O=  @D,EFG
3l#07 5-820M 8;08,8;0=D30%%4 6
8,8;00 3l#?+ CD-0_  4>;380M%
#>0 6 D60;=O?W C0W0#8 6 *0?>?/0,Q
#>0 6 0_  M BG0WD03 3l#e8_FG8DlUf?+ C
D-0`0#>008%0,[V?C48 K C0W0M
,,1-`08+,7=OB
− 6 8,8;0F20 10\ C0W0'0*0?+D2#10@D1
0;=OeD18;0 P4?+D2D_;DN 20Uf`+F*D6
ZF*50Ee8Dlt ==,fB
!
− g-36006>00M% P 6 78,8;0 CI€/0
=082 0 @="=0*0F& ` 10\4?C <=#>0
?W#= P07 5-820M 8;08,8;0B

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t% *L?W#0%0j07 5-820M 8;08,8;0 R#>0,7*
Z
− 7 0S,"8, C0_?A 2 %3Y06 ?>‚3BJ?C?W0%
F20+=* 10\0j3-,EFG+=*07 0S,"8,4?+
D1#=*2[-8+D0B
− J5 ;#4DA3?+D20@005/00;0jDC?*0D0[-070B
− &L06 ?> P#10@/0=6,64=O 04
 #420?>8B8BBB
− gC "??'>40@5-[-N40@o(#4
0L?+D2=*4D1B
− 60 R B%8L* 0%=/0T K0 C0W
* ?O 5b+52363D6 B
:.>.=.aD-b9Vh`iDDMN7-RTD7KUEVRE45--WD.
6 8,8;0F20 10\ C0W#=% 10\5b+ 6 D6
Z5?@0%6e`8Dlf4D,"3-=6 C8Dl400W?>  \
850E,^."#;38 V0W,"[?@0%CBg?8>0L400W
?> R08?O 30],]0>0*0C0F* C*00_?> Bc> 07
=%D08055W#10>04?"#[#0*0=i.%#4=##/0
"5b>5 P055W#1=#  K5&3"-4>05&36*4,"
0B

:.j.8DhbR7TP5--fk597l4DaD-b9Vh`iDDMN7-RTD7KUEVRE45--WD.
:.j.:.%5--fk59DMND8DhbR7TGm7no
20?>Z6  3l#07 0S,",Q 0V?75+8204*
20?># K C0W0'0*0V?7>BK C0W &?O 20?>=%0


B
c>l#Zg`F*50E0j?>l# P#10@D1-`+0

KBg`F*,0`0j?>l# C06 ?>/0=006 FG"?> 0 V
0W 10\ P KBc>l#0_ O3  K C0W3600W0% V0W 1
0\8,"*=0\0> 80S 3l#40@?>l#070/0b#0D-
!o)mB
6 07D6 Zt% * 6 0720?>4?>l#0j6,64C4# <
-`D1`006 FG P 6  3l#B#>0,7=* 3l#0@5&
3"PF06 FG P6,6#A00@8 @?> ,6 Bn <06
FG/0=03*#8"52 P 3l#43 6  3l#A30@#0j
/0F^5&E01F?C2[-D1 B
:.j.=.%5--fk59DMND8DhbR7T-pN-WD
3Z3 P#10@# 6  3l#07 0S,"8,8;00'08360
0W070=#10@D+#5%0 V0W 10\4,"*BD-3070_ 0\
0> 80S=* 3l#D6 4010@b#0D-3ƒ4)o!4)=
070B
6 07D6 Z3 P#10@ 6  3l# R &06 ?>
P#>0,7 /0 C0 6 07 5-820M 8;0CQ ?F\8#>0,7 /0D_ 
0_ ,0`D6 B
:.q.8DD-M59$`fgDEFcd59`LEP5QRS77-RTD7KUEVRrP]G[7-IDGm7.
:.q.:.i7ETG4s-Rt5E45-rO]7F`4L5-u5-c4[7EVR-v4
Clostridium brevifaciens.
Clostridium malacosomae.
Bacillus cereus.

Bac. thuringiensis.
Bac. thuringiensis var. entomocidus.
Bac. thuringiensis var. galleriae.
Bac. thuringiensis var. isralensis.
Bac. sphaericus.
Bac. sopilliae.
:.q.=.i7ETG4s-Rt5s-w59E45-rO]7F`4L5-u5-c4[7EVR-v4

• Serratia marcesecens.
• Pseudomonas aeruginosa.
• P. putida.
• Proteus vulgaris.
• Proteus mirabilis.
• += P AcrobacterB
• += P Cloaca.
:.x.8DnWN47-RTD7KUEVRE45--WDJ-Xr4b57Ky57-C7Kfz59
:.x.:.8DnWN47-RTD7KUEVRE45--WDDp59R259TDG4s-Rt5
  3l#  t0  et =,,  0],,fB  |  20  #4    3l#  t0  et ==,
0],,f?d?O % H0S#!BV( 3l#t0;3Dl8>
?&?d D0[-07003R0_2#8?'?78#>0,7,"* _
0%?'>,".5#0N4,".6#4,"0V4,"*514,"?B6 =Q
,-3l#0V#* C0%0&0@D6+ot0(r4r„t0
z]0)rJ4…50.F*5>0„…50.F*F&  1?A BBB
g1238,Q HFGec*Q Vf <?d% H
8?( 3l#,Q t5 8t, CD-3R0S(520@
A30%=K=?7#8b8 6=6B3l#t5 ?O ,-./00S#>0 P8
Dl C,†`?&3V4 CD-0%F208H  ,M3600W P,O/#"
52?7#8bBR 3l#t,=,-3l#?O  0./00S#>0,7=0M 8;04
CD-D_ 0_ 0_D652 6=6=Ke?*1fF/# ="B

u5-:,07 0S,",Q  C'7 0S8Dl0%0&0@

/=,$%&'(')
$'$
=.:.-bJ-t\7-RTD7KUEVRE45--WDEP5QRS77UG4s-Rt5Bacillus thuringiensis
=.:.:.$anfgDGHD-bJ-t\7
t0?O 36028#Fu,v0Bq4=?0%3"=A3?O  P
8Dl"52 0b#8?#4t0?O ?DI#>0=*07 0S,"

080Y H w‡B4,-3l# P P?O ,EFG?W
0*07 0S,",Q ?78+ 10\"52D6 B
7 0S,"8,t0et =,,0],,f0> C#0S,",Q 4 C
'7 8Dl43Y06 FGF20,">8L2?78 6 =Q,","
7=64,"0V4,".4,"D4,"0*3Uq"D3-07 ,^S,
8@8 0,~
=.:.=.4s-Rt5Bacillus thuringiensis
t0=0M Dl,50ED_D15N05> 4>##FV4D_ 
0 oˆ# C3P0%##D1F405?H%=^.300S iB
(
u5-=.Dl Bacillus thurigiensis
P59:,n>0,7=t0 _?O HFG0,-./0 3l#
$ y59W4 Zo-4[R
: Bacillus thuringiensis 7
= Bacillus thuringiensis subspecies aizawai 7N
> Bacillus thuringiensis subspecies darmstadiensis 7c
j Bacillus thuringiensis subspecies entomocidus 7{
q Bacillus thuringiensis subspecies israelensis 7s
x Bacillus thuringiensis subspecies kurstaki 7s]
| Bacillus thuringiensis subspecies konkukian 77
} Bacillus thuringiensis subspecies tenebrionis 77{
~ Bacillus thuringiensis subspecies galleriae 79
=.:.>.-R7Ku5-ET59DMN7
0j,7 P8Dlt0 5?*Z0W,F•450E4
50E800WZ
− -LE45-cf•59
W,F•F*[]4?l0\4D_ 0 4(o4ˆ#.o)ˆ#45N0#
#FVD>##807 >#B$1#Q .[V?>A
D1?>BK0@0'0*#>0 60WA  60W=+BWFF•
,,-0]DW3" B0@Dy,,-0@ C(44U0WF

F•=+0 iB$K 8Dl,0`40?Y /0+F
1 /0%#10@B
− N59rO]7F
g 6 0W8Dl4#>00?0 V0Wj050Ej5
FG 4 R?Dj00Wj0BcC=?*50E4`?*
50Ej0HF40V0W,F•B
− O]7FGO745-7-L
g50E3600W?#>0?*?C4 K,^H04-3C
50E800WBg_ 0 50E4o4ˆ#.(ˆ#Bt0E`F*< C0W?+
D68 6 ?+D2#10@5/0=OB3-#8Dl0@?O 5-[-`
F*50EB0W0@ CD_ 0 0?YD-4.(ˆ#4j04 < C

=*j0R4j5FG 0\0]=8=*#10@B0W=#>0=*30]
e /0F20,"f C2[- PB
=.:.jiD7TGODaD-bnVh5-4€\`iDDMN7.
=.:.j.:8Dn]v4`iD7TDMN7.
Dlt0"52  10\[ ?@0%6Bt0E-#?X
?,M,,- P8Dl0 V0W8;0 P=# 10\ 04,07 _
=# 10\ 0 C=*= /0?> F8Dl,4?C= 6  /0?> 0
0Wef?O #d65` 6 ]D6 e?" <=#>00 6 F/2
F\?W3"=* 6 C#t08 6  /0?> 3"-05ef C06 ?>%
^80YO3 \=#006 FG P00W?> BC# /0?> 5'#
6 *?> 07e,-3l#00 P8Dlf‰4Š4‹B
o*?> 07‰=#>0=*]Œ#]3,3=3,]?O 000 D50E8
00W?> ?O j04 K C06 FG"%,M3"} 6 #10 V0W
10\5&06 ?>B

brO]G4s-Rt57Gl4745-7-L
(crystal)GOrO]7F(spore)
u5->.50E8•-3C50E800W?> 

o*?> 07Š==*?> 07 Pt0?O % HDx0;0Bc> 07 C0_
5+204?O 0*0 D00W?> j0B*?> 07Š C /0K 0V
0M    ‡4  C  06  FG    0  8  ‡4  =#  H    *0  ?>  P
‡•Ž3=#],]B\800W?> 4*?> 07."#;3800V P
10\4? 6  V[=#00_?>  P8DlD?8 V0W 10\B
2[- P*?> 07Š0W2a0%?70O," P 10\ &06
?>4=# -[60j=>0.6 4A "F&0@0[60j3600W P
,"B6 FG P?> 07 R3G0> 8=+=O8 6 0H ,EFG?W"
?> 40@0j"?> [0%# 2[-#*V=[?@0%6B
o*?> 07‹„?> 07 <==*3,3=3,]06 ?>=%3,03=3F4
=#36} 6 #105 P,"*D5&#t0B
q8C#?> 070%400W?> 0*8=O=V8 C2[-
_"?>   10\B0W R?O Q=>?> 07•B0W?> =
#>030]D00 CD_ 0 D6 5200y0SC#B03
30]400W R+%07D6 4n4…]4q4•‡=U0W?>
?O 5N0?0*DD`?[60jj050E4?O F0_ =x0S _
30]005 C=%2#;0008#50EBD?O D00000W
C+F*D6 4 P=j0B0WD100 4A
6  /0L Ve =‘#4]0=4],0]f4 C0W00FF& D+#B0
0W C0WY?&0 6 FF&  C3*#83D6>eo(f4 C0W5&50_
0 F0 .]0 4 =0}"B* -#820?> , C0_
&20/0?&e`)

 C0WL?O 4

 C0WL0(3K0fB
c> 07•'# C8\ H ?C=Z
o\u=#>05C'#! i.N‰Bn>08A 0/0 - 6  i C0W 
8#05>040* 6 =i40S?C 6  C0W[=*0MFB
o\uu HF-ŠD1,0V0M8\ND6% P

=5=#F& B\ C80RN80G0W0%5+#A0055W#1>0B
o\uuu C2#8G5-82?> 07?d?O *06D15&3"}5`
30],]`>0
)
B
 /0K 3H 0*38;4>?> 07•=%D0?A 28 6 0G0W0%
#055W#1>0 P,"4"6 ?>F" +?O %`0%B_
?+=#%0_?A 2/0 02[-06 ?> Pt0=%,"*BJ?C
3Y06 FG Pt00V?7‚340y0S=*00W?> # 6  Pt0 C06
FG8 6 =*," PC# 10\ P0> 5>$]3F30]B
cd CV)]#d6 30]00W?> ?d?O -#d 3k33"
=* 6  /0?> 803)C#FM0%700j0M]4
?O %`5-,Z
P59=,6 C#00WF20 10\
] j  F*  0
0W
g7  =O
30]  0
0WeDJf
c70OF20
ue+C#3GZ‡ef4‡e5f4
‡e f4t44J4w4…4f
j0 o 6  /  0\
68-
uueC#3GZ‡4t4f jD7 o! 10\5> 6
8-45> 6
uuueC#3GZ‡4t4f j3’A !o! 10\5> 6

u5-j.t0E8>?> 07• PBacillus thurigiensis
81?&j H

uuueC#3GZ‡4t4f j0 !o 10\ 6
ouz cF* )o( +=
=.:.j.=aD-b9Vh`iDDMN7.
V "?>  P00W?>  \850E."#;38 V0W,"5b 
?@0%6D,"3-=6 C8DlB?+D25j0@400W?>
D10Bg?8>0L P,"4V C3D+# e“4)f=# 00W
?> 0B%F*0 3-F**0?>BJ*0+?> 07e C
D_ 0 0S)oDJf?O 30],]0>0L P,"*060
F**00_eoDJf?> 07•Bc> 07=%D0805#10>04?"#
[#0*0=i.%#4=##/0 "5b>05 P055W#18
=# K5&3"-4,"S85& 0?CB30>05&-#.75b
83>#1000VBc>30/3 3k350E-##4."#;38;0
P8 7 \" 0B
!
u5-q,V 06 ?> P00W?> ?78,"
o‡Zq"=6 C8Dl 00W?> 850E."#;38 V0W,"B
?+D25j0@400W?> D1R0B
otB{60jR000W8*0C /0?> Z `>0LV C3
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#?TB


Giống Gốc
Sản xuất giống
cấp 2
Chuẩn bị môi trường
Khử trùng môi trường
Lên men trong khoảng thời gian
48-72h, pH=7, nhiệt độ 30oC
Hoàn thiện chế phẩm
- Nghiền lọc bổ sung phụ gia
- Sấy khô
- Đóng gói bảo quản
Sản xuất giống
cấp 1
Thổi khí
Lọc và ly tâm
Thu sinh khối
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0j,"# 0B
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3-3=*#>0=L?WF20,"#`B
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(
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36* P,"B
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5%0 V0W "0'4 _8j0D10W5&3"}5`06 "6,6#A0
0@00E*4 <D10W5&E01F #8 0  "B
_8j8;/0+= "0't0?d?O ?8,EFG14$K4
t1D0"4  U8#>0,77 "0'D6 B
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