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23
Sơ đồ 1.2.5 Quy trình nuôi cấy sơ cấp
Thu nhận mẫu mô
Cắt nhỏ
(Chọn lọc mẫu mô quan tâm, cắt bỏ phần mô chết)
Cắt nhỏ
(Mảnh nhỏ để nuôi)
Tách TB bằng cơ học
(nghiền, ép)
Tách TB bằng enzyme
(ủ,…)
Nuôi mẫu mô sơ cấp
Thu nhận tế bào mới
Nuôi mảnh mô thứ cấp
Trypsin lạnh Trypsin ấm Collagenase
Li tâm
Nuôi sơ cấp
Cấy chuyền
Dòng tế bào
Tái huyền phù
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25
