Analysis of regulator of g protein signaling (RGS) function in growth, development and pathogenicity of magnaporthe grisea 2

Figure 16
WT TMT1398
Figure 17

A





B

MDDTSRPDQVFKITSTQGIDHNNKPTAPSEVVNEKSHAALSSAHLHSTLPAPLPDTTAKPGSRKSLL
LQQHQQQKSTTESTNAAPDSKPQRAGGIFAYAAAALDRTLSESRNIRSRPSLNRLSVSVLPESTTTT
SSPRQPTGGNNGSSSPEKKARNRASVSNLRLSSLGTPPSQPYNDTDPSQPAPVHVQRLSNKMHQTS
SRLLRMTDDDRPFTKDFKDLFATLIVSMLPLTPHRVRLTKIDHTFLSEDAINNLGSLKFSQSNRMPD
PKDVARIVTTTTTTTFSMAKEMARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILD
SFASRNGIQDRQIPEVVGSSLAQLVTLERDPATDKLVKDFHTIEVIFGRFIGIHGPNVKSSVSSADSD
SLSDYRDGLTGVKMAPERKVNGKIHKDTFTGKAASEWLMDCCTTVDRREAVEIASLFVEYELIEA
LQQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGSVPRGRASEGDATHSATHRHGVARDSNT
QRMDKILNDAALRLLFRENLRETHCEENLSFYLDVEEFVRLCRSAVRQAQLAQSSPANSAEAKKQ
ALDGVKEVMAQAYGIYNAFLAPGSPCELNIDHQLRNNLATRMTKAVGQDSAMVETLQEVMALF
EDAQNAVFKLMASDSVPKFLRSRRYEHLLKNYDFDSVSLESSPRVPERSQSRSQRL


C



Figure 18
Rgs1 1 MDDTSRPDQVFKITSTQGIDHNNKPTAPSEVVNEKSHAALSSAHLHSTLPAPLPDTTA

Cprgs-1 1
FlbA 1 MPTISSTPLDQGSPPSSQHHHPSCHSHTSTTSVPVSSTPADIPSSSIISPLTSPAPTTSS
Sst2 1 MVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKEKKHSSD

Rgs1 59 KPGSRKSLLLQQHQQQKSTTESTN AAPDSKPQRAGGIFAYAAAALDRTLSES
Cprgs-1 1
FlbA 61 TTTTANTTATTTTSFTGSVIGSISRRNRRSFAALAREKTSNALANLSAIGSTTSSSLRTS
Sst2 51 TKSFLLTAFTKHFHFTFTYQEAIKAMG QLELKVDMNTTCINVSYNIKPSLARHLL

Rgs1 111 RNIRSRPSLNRLSVSVLPESTTTTSSPRQPTGGNNGSSSPEKKARNRASVSNLRLSSLG-
Cprgs-1 1
FlbA 121 TSSGSLSKHSRKPSQISTHEIAGVSPLTPPLSDESTSSNQSSPACIETSEVESQQSLSGR
Sst2 106 TLFMSSKLLHTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSSFNSMK

Rgs1 170 TPPSQPYNDTDPSQPAPVHVQRLSNKMHQTSSRLLRMTDDDRPFTKDFKDLFATLIVSML
Cprgs-1 1 MHQTSSRLLRMTDDDRPFTKDFKDLFATLIVSLL
FlbA 181 RRHTLQRNSPISEQQLEAGFAALPSKMHQTSSRLLRMTEDDRPFTKDFMDLFSTLMVSLK
Sst2 166 LFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEYTNNDDTF

Rgs1 230 PLTPHRVRLTKIDHTFLSEDAINNLGSLKFSQSNRMPDPKDVARIVTTTTTTTFSMAKEM
Cprgs-1 35 PLSAHRVRLTKVEHTFLSEDAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKDM
FlbA 241 -LDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEM
Sst2 226 TFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTF

Rgs1 290 ARSMLQRFLEARLIESADGKYQQVYTMRGTVWQLTPKGISILDSFASRNGIQDRQIPEVV
Cprgs-1 95 ARSICQRFLEARLIESADGKLQQVYTMKGSVWQLTPKGIAILDQFCSKNGIQQKQVAELI
FlbA 300 ARSVCQRFVDARFIEPVDGKALPIFPLKGALFQLTPKGINILQRFCQRNGITARHVIDVL
Sst2 286 GTSKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPVLLQPSRTDKKK

Rgs1 350 G SSLAQLVTLERDPATDKLVKDFHTIEVIFGRFIGIHGPNVKSSVSSADSDSLSDYRD

Cprgs-1 155 G PALTQLVSLERESSTDKLQTDRATMEVVFRRFTGLKGLNIKSNVNAADSESLSDYSD
FlbA 360 ESPRNTMQLVNLERDTETDKLSHDRATIEVIFRRFAGQDGPNVKSSISTSDSDSLSDYSN
Sst2 346 FQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLDDSE

Rgs1 408 GLTGVKMAPERKVNGKIHKDTFTGK-AASEWLMDCCTTVDRREAVEIASLFVEYELIEAL
Cprgs-1 213 GLTGVKMAAERKIGGKTYKETFTGK-AATDWLMDCSTTVDRRETVEIASYFVEFGLMECV
FlbA 420 GLVGVKMAKERKINDKIYMNTFTGK-AAVDWLMDCSTTIERRETVLIAELFVKYGLITML
Sst2 406 GFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKK

Rgs1 467 QQDRSHIQQFPGCQVFQPTKHAIYHMTSKGKDMINGSVPRGRASEGDATHSATHRHG-VA
Cprgs-1 272 QQDRAYTAQYPGSQLFQPTKHSIYQITPNGKDLINGMNSRGRTSDAESTPRDHKPNEKLP
FlbA 479 QEDKGYPQPDASIVVFQPSKYAIYGITERGQRVCG WIARDKSRETFYDNRGMP
Sst2 466 LIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGSPYQLNIHHN

Rgs1 526 RDSNTQRMDKILNDAALRLLFRENLR ETHCEENLSFYLDVEEFVRLCRSAVRQAQLAQ
Cprgs-1 332 RDSNTQRLEKILSDPALRLLFRENLR ETHCEENLSFYLDVDEFVKQCKLAIRNAQKNP
FlbA 532 KDSNNARLNHILQDPALRLLFREFLR FSLCEENLSFYLDVSEFTAAYHKSEKMGAFKK
Sst2 526 LRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKN

Rgs1 584 SSPANSAEAKKQALDGVKEVMAQAYGIYNAFLAPGSPCELNIDHQLRNNLATRMTKAVGQ
Cprgs-1 390 STTS MDGIKEIMAQAYGIYNAFLAPGSPCELNIDHQLRSNLATRMTKAVGQ
FlbA 590 ADAVRETLAAAYGLYNAFLAPGSPCELNIDHALRNSLASRMTKAVGD
Sst2 586 LSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVES

Rgs1 644 DSAMVETLQEVMALFEDAQNAVFKLMASDS VPKFLRSRRYEHLLKNYDFDSVSL
Cprgs-1 441 DVAMIDTLHEVTALFEDAQNAVFKLMASDS VPKFLRTSKYESVLKNYDFDSVSY
FlbA 637 DESMFKSLQEVVQLFEMAQTSVFKLMSSFTDEDQDSVPKFLRDPKYAVVLQEHDVDLIGA
Sst2 646 FPATLKVLRKLYPLFEIVSNEMYRLMNNDS FQKFTQSDVYKDASALIEIQEKC-

Rgs1 698 ESSP RVPERSQSRSQRL

Cprgs-1 495 PVG KGLDRSQSRSNRK
FlbA 697 PRAYSPTPAPVPERSMSRSARS
Sst2



Figure 19
Figure 20
A
HPH1 RGS1
B
WT
rgs1D
Complemented
Rgs1 OP
Figure 21
_
A
WT
B
Rgs1
OP
rgs1D
Comple-
mented
Figure 22
_
Inductive
Non-inductive
Complemented

WT rgs1D
Figure 23
Figure 24
Figure 25
Rgs1
MG00990
MG03146
Figure 26
MG09618
MG03726
MG08735