Đánh giá hiệu lực của vacxin amervac PRRS đối với chủng virus PRRS gây bệnh động lực cao ở việt nam

KHOA
HOC KY THUAT THO
Y
TAP XXI SO 1 - 2014
i0Sl)iln
cifu fefipa
toe
DANH
GlA
HIEU
llfC
CUA
UACXIN
AMERVAC PRRS
001 VOll
CHUNG VIRUS PRRS
GAY
RENH
DONG
llfC
CAO
A
VIET
NAM
To
Long Thanh', Nguyen Hoang Bang', Tdng
HS-u
Hien',
Bach Qudc Thdng', Daniel
Torrent^
TOM TAT

Dung vacxin AMERVAC PRRS khdo nghiem tren
Ion k6t
qua cho thiy nhu sau: Vacxin
nhu^c
doc AMERVAC PRRS do
hSng
Laboratorios
HIPRA,
S.A san xuit dam bao an toan khi su dung.
Vims vacxin khong truyen ngang tir
16
thi nghiem sang
16
doi chung va c6 tac dung kich thich sinh
dap ung mien dich khi
Ion
bi nhiem virus cuong doc va cho dap
iing mien
dich se manh hon. Dap ung
mien dich do vacxin tao ra co tac dung lam giam muc dp tram trpng ciia benh khi cong voi 2 chung
virus PRRS cuong doc
ciia
Viet Nam thong qua kiem tra benh tich dai the va
vi
the tren cac ca quan
npi tang,
dac
biet la a phoi. Lo diing vacxin ciing tang trpng hon so voi 16 khong
dimg
vacxin khi

cong cudng
dpe.
Dap ung mien dich co dupc nha vacxin da loai bo hoan toan virus trong ca the
Ipn
v^o
khoang 2-3 tuan sau khi bi nhiem.
Tir
khoa: Lan, PRRS, vacxin PRRS, khao nghiem, cong cuong
dpe
Evaluation of efficiency of Amervac PRRS vaccine for the High virulent,
pathologenic virus strain in Viet Nam
To
Long
Thanh,
Nguyen Hoang
Dang,
Tong
Huu
Hien,
Bach Quoc
Thang,
Daniel Torrents
SUMI\/IARY
Live AMERVAC PRRS vaccine produced by
HIPRA
Laboratory, SA (Spain) was used for
testing on pigs raising in
Viet
Nam The tested result indicated that
this

vaccine was safe in use.
Vaccine virus did not transmit horizontally from experimental pigs to control pigs and had the
effect in inducing immune response at higher level when the pig was Infected with challenge
virus.
Immune response induced by vaccine had the effect
in
reducing the serious level of
disease when challenge with 2 virus strains of Viet
Nam.
This result showed clearly when testing
the gross and histobiological lesions in internal organs (particularly is lung). The vaccinated pigs
also increased higher body weight compared to the control pigs when applying challenge test.
Immune response induced by vaccinating AMERVAC PRRS vaccine had removed the entire of
virus In pigs in 2-3 weeks after pigs were infected with PRRS.
Key
words:
Pig,
PRRS, PRRS vaccine, Testing, Challenge test
'
Trung tam chan doan
thii
y
Trung uong
'
Cong ly
thii
y xanh
^
Cong ty
Hipra

S.A, Amer Tay Ban Nha
KHOA HOC KY
THUAT
THU
Y TAP XXI
SO
1
- 2014
I.
DAT VAN DE
Hpi chung roi loan sinh san va ho hap
ciia
Ign
(PRRS)
th8
doc luc cao do virus PRRS
(PRRSV)
thuoc genotype II gay ra. The benh
nay xuat
hien
tai Viet Nam nam 2007 va da gay
nen nhung
t6n thSt
kinh
t6
nang ne cho nhirng
nguai chan nuoi lan trong nhiing nam sau do.
Ve lich sir, PRRSV genotype
1
xuat hien lan

dSu
tien tai Ha Lan nam 1986, gay nen PRRS
the kinh
di£n
hay con gpi la PRRS the doc luc
thap da tham nhap vao Viet Nam tir cuoi nam
1996 dau nam 1997. Nhirng nam sau do, mpt
s6
loai vacxin, trong do c6 vacxin AMERVAC
PRRS da dupc dang ky sir dung tai Viet Nam.
Vacxin AMERVAC PRRS la vacxin genotype I
da duac su dung ke tir khi dang ky tu nam 2005
cho den nay.
Viec sir dung rpng rai vacxin AMERVAC
PRRS (genotype I)
d§
phong
ch6ng
PRRS thd
doc luc cao (genotype II) tai cac dia phuang
doi hoi viec danh gia kha nang bao hp ciia loai
vacxin nay vai chung PRRSV the doc luc cao
dang
liru
hanh tai Viet Nam. Day cung chinh la
muc dich cua bai bao nay.
IL NGUYEN LIEU VA
PHU'CTNG
PHAP NGHIEN
ClTU

2.1.
Nguyfin v3t
lieu
- Dpng vat thi nghiem: Chpn 16 lan con 4
tuan tuoi, khoe manh va am tinh vdi virus PRRS,
khong hem bdt ky mot loai vacxin phong PRRS
nao.
Lan duac nuoi va tien hanh thuc nghiem
tai Trung tam chan doan
thii
y TW.
- Vacxin
nhupc
doc AMERVAC PRRS do
hang Laboratorios HIPRA, S.A san xuat, dung
cho lan 3 tuan
tuoi
tro len.
Virus cuong doc gom 2 chung: Chiing
virus PRRS so 21 do cong ty HIPRA cung cap
CO ngu6n goe
tir Viet Nam nam 2008 va chiing
vims PRRS ky
hieu
VN07196 cua Trung tam
chan doan
thii
y TW.
- Kit
ELlSAphat

hien khang
thi
PRRS
- Kit chiet tach
Qiagen
RNeasy, kit nhan gen
Invitrogen
SSIII
One-step qRT-PCR system
(Cat 11732-020).
- Cac hoa chat va nguyen lieu can thiet khac.
2.2.
Phucmg ph^p
nghien ciru
- Bo tri thi nghiem:
% ai livti ^a plmong i>li:'ip thi ii)?;lii
I
1 il
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n.,™«„
.
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KHOA

HQC K? THUAT THO Y TAP XXI S6 1
-
2014
Theo d5i
Ipn
sau khi
tidm
vacxin
d^
danh gia
tinh an toan, bieu
hi?n
iam sang
\k bi^n
ddi than
nhi?t
tir ngay
diij
32
dgn
46. Can
Ipn,
theo d5i
trpng lupng vao cac ngay thir 0, 32, 39, 36 va
55.
LSy
mau vao cac ngay thu
29,35,
39,42,46
va 55

d6
xet
nghi?m
ham lupng virus
huy6t.
Xet
nghi?m
khang
th6
khang virus PRRS vao cac
ngay thu 0, 29 va 55.
M6
kham
kigm
tra benh
tich, cham diem phoi tit ca
Ipn
vao ngay
thijr
55.
2.3.
Cdc phinmg phdp
xit
nghl|in
- Diing phuang phdp
ELISA
va
IPMA d6
kiim
tra khang

thS.
- Dimg phuang phap Real-time RT-PCR
d8
kiem tra virus.
- Dung phuang phap
m6
kham va lam
tiSu
bdn vi
thi
de kiem tra
b8nh
tich dai
th8
va vi
thi.
- Phuang phdp ddnh gia an toan
ciia
vacxin:
Theo doi mpt tudn sau khi tiem vacxin, nlu
khong
CO
phdn irng bat
thuong
nhu:
mSt
moi,
chdn
ho^c
b6 dn, tdng than

nhi?t
vd cdc dau hieu
b?nh
1^
khdc Id an toan.
- Phuang phdp ddnh gid
hi$u
luc
ciia
vacxin:
Hi^n
tai,
Vi?t
Nam chua c6 chting virus PRRS
c6 khd ndng gay
chit
100%
Ipn
man cam nen
vi^c
ddnh gid
hi?u
qua cua vacxin PRRS can
phai
kit
hpp
giiJa
chi
d6u
bao hp sau khi cong

cuong
dpe
voi cdc chi tieu khac bao
g6m:
Bilu
hien lam sang, tang than nhiet, benh dch dai
the,
vi the a
ph6i,
tinh trang nhilm virus mau va
kiem tra dap irng mien dich bdng
phucmg
phdp
huylt
thanh hoc.
III.
KET
QUA
VA THAO
LUAN
3.1 Ket qua theo doi an
to£kn
sau khi
tiSm
vacxin
Theo doi mot tuan sau khi tiem vacxin 8
Ipn
khdng thay co bat ky cac phan img bdt
thudng
nhu: met moi, chdn hoac bo an, tang than nhiet

va cac dau hieu benh Iy. Nhu vay, cho thdy
vacxin AMERVAC PRRS la an toan.
3.2 Ket qud theo doi sau khi cong
cu^g d$c
3.2.1.
Bieu hien lam sang va thdn
nhi^t
sau khi
cong
cir&ng dpe
Lpn sau khi cong bang 2 loai virus PRRS
vao ngay 32. Tir ngay 33
d6n
ngay 46 theo doi
nhiet
d6
vd cac bieu hien Iam sang va cham
diem.
K6t
qud theo doi Iam sang dupe the hien
trenbiludo lava
lb.
Nhan xet ve lam sang:
* Cong
cu^g d$c
v6i virus PRRS 21
-,c
2,0
1
1.5

J '•''
Q
0.5
—• ^
1
/
X\
/ ^^*^ ^ *\
/
,y^^^
\
"\'"
J
y*
\
^
-^V)t1
-•-DC-1
^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ .^ ^ .^
Bieu
do
la.
Bieu
hi^n
tam
sdng
cua
Iqn dirpv
cong virus PRRS 21
KHOA HOC

KY THUAT
THU
Y TAP XXI SO 1
- 2014
- Sau khi nhilm virus PRRS 21, lpn 16 1
(VX-1)
va
16
2
(DC-1)
bit ddu co
nhimg bilu
hien ddu tien sau
1-2
ngay. Trieu
chirag
thudng
thdy Id lpn giam nhanh nhen, an it hon
miic
binh
thudng, lpn ddi chung an it han lpn tiem vacxin.
- Trieu chung tien triln nhanh va nang han
d nhung
Ion
ddi chiing. Tir 4-8 ngay sau khi
nhilm virus
Id
giai doan
b?nh n$ng
nhat. cham

chap,
it van ddng va gidm dn. Mdt s6 cd trieu
chung thd nang nhpc hoac tim tdi d tai. Co
1
lpn
(s6
11)
d
Id
ddi chung cd bieu hien benh nang
han cac con cdn lai trong id, the trang rat met
mdi,
thd ddc "kieu chd ngdi" vd co hien tupng
tim tai. Ddi vdi nhung lpn
Id
vacxin, cac
tri?u
chung tren khdng bieu hien ro va thudng chi
keo dai 1-2 ngdy, khdng
lau
nhu
Ion
ddi
chumg.
- Tir ngay 8-10 sau khi cdng (ngay thu
40 ttd
di) cac trieu chiing
bSnh
giam ddn, Ion nhanh
nhen ban, an khde. Nhdm Ipn vacxin den ngdy

thu 42 da khdng con the hien trieu chiing. Nhom
lpn ddi chumg phuc hdi cham hon 3-4 ngdy.
Rieng
Ion
sd
11
thudc 16 ddi
chumg
bieu hien
benh ngay mdt nang va chet vdo ngdy
thii
44
(ngdy
12
sau cdng).
* Cong cudng doc vdi virus PRRS VN07196
2.0
c
»
15
£
E
10
V
05
00
—•—
r^^~~~
/
/

/—
/,
/
/ \ ,
"\^
\
\
\ \^
V^
\
^\,
\
.^
<^
fij"
4>
4
^
t^
^
t^
1^
' ^^^ ^^^ ^^^ ^^^ ^^^ ^^^ ^^^ ^^"^ ^^^
Bieu do
lb.
Bieu
hi$n
lam sang cua
Ion
dw^

cong virus PRRS
VN07196
- Lan
Id
3 va 4 sau khi cong virus PRRS -VN
07196
bat ddu cd bieu hien benh sau khi nhiem
virus 3-4 ngay. Tien trien benh cd the quan sat
dupc la hien tupng moi
m8t,
cham chap. Tuy
nhien lupng cam tieu thu van khdng gidm, hoac
chi
giam
khong dang ke. Lpn doi chiing co bieu
hien benh nhanh ban vd nang ban so voi nhom
vacxin.
- Qud trinh benh keo dai trong khodng vai
ngay (tir ngay
thii
37
d6n
ngdy
thii
42)
Id
ndng
nhat, vdi hien tupng Ion met moi, thd khd vd
ludi
van ddng.

Miic
an d nhimg ngay ndy giam
han so vdi trudc do vd lpn tiem vacxin an nhieu
hon
1pm
ddi chiing. Ld 4 cd 1 Ipn ddi chiing (so
8) cd bieu hien
b8nh
nang horn nhung con cdn
lai trong cung
Id,
thudng nam bep mpt
cho.
Lpn
ndy
chit
vao ngay
thii
43
(tiic
la
11
ngay sau
khi
bi gay nhilm bdng virus PRRS VN07196).
- Lpn
Id
vacxin bdt ddu
hit
cac

tri?u
chiing
vd phuc hdi sau khodng 10 ngdy nhilm virus.
Lpn ddi chiing cd thdi gian phuc hdi cham hon.
KHOA HOC
KY
THUAT THU
Y
TAP
XXI SO
1
- 2014
Nhdn xet ve thdn
nhiet:
-vx-2
-DC-2
-^
4-
-^ •^ -
"'•
J^
^ ^ ;^ ^ ,^ ^
^ .^ .^ .^ .^ ^ ^
.
[3-
I?
ftp-
li^
^. ^ ^ ^ ^
Bieu dd

2a.
Diin bien than nhiet cua lan
du^
giy
nhi$m
virus
PRRS
21
41.0
O
40.5
1'
40.0
c
S
39.5
1-
39.0
38 5
^/
r-^
r^ y~^~^^^^
It
^~~ 4 ; ,__ _^
J/>
^^'^^—
^^ -^^ /^ /^
4>
4
^^ ^^ ^^ ^^

>^
J>
^ti^
>*^
>*
.^Q.
.^Qp
.^'
.^o.
.^ .^ .^ .^
.^o.
^
.^Qi .^Q>
^
^Qi
^Q,
VX-1
BC-1
Bleu do 2b. Dien biin
thSn nhi$t ciia /pn {fut>v
giy nhiem virus PRRS
VN07196
Bieu do 2a vd 2b d
tr8n th6 hi8n
dien bien
thdn
nhi?t
cua lpn sau khi nhiem 2 loai virus
PRRS.
- Than nhiet trung binh bat ddu tang hi ngdy

thii 33 (virus PRRS 21) bic la sau khi cdng
cudng
dpe
1
ngdy.
- Lpn s6t cao nhdt vdo giai doan ngay
thii
35-41
vdi than
nhi$t
trung binh co
liic
len tdi
tren
41''C,
ddc biet la cdc
Ion
d 2
Id
ddi chumg.
Tren bieu dd cho thdy virus PRRS 21 gay sdt
vdi thdi
gian
nhanh hon (sau 3 ngdy, than nhiet
trung binh tren
40°C);
tuy nhien virus
VN07196
lai gay sdt dai han (den ngay
thii

44).
- Qud trinh sdt gidm ddn tir ngdy
thii
41-42
vd lpn lai trd ve
miic
than nhiet binh thudng.
Rieng lpn sd
11 (Id
2) va lpn sd 8 (16 4) cd
hien
tupng giam than nhiet xudng dudi
38''C
va chet.
KHOA HOC
KY
THUAT THU
Y
TAP
XXI
SO
1
- 2014
3.2.2. Trang thdi virus huyit (Xem
bdng
1)
Bang 1.
Mipc
do virus huyet tren Ion (tinh trung binh)
L6

VX-1
BC-1
VX-2
DC-2
Ngay 29
40,00
40,00
40,00
40,00
Ngay 35
29,61
26,69
27,95
26,01
Ngay 39
27,15
24,29
26,93
24,32
Ngay 42
29,98
26,55
28,76
26,20
Ngiy
46
34,99
32,39
33,16
33,75

NgjiySS
37,72
37,23
36,63
33,65
(Ghi
chit:
Cl la ngudng vong
cUa phdn
img Realtime RT-PCR, ddnh gia
luong
virus cao hay
thdp,
Cl cang
thdp
luang virus cdng nhieu va
nguac
lai. Thong thudng phdn iing
RRTPCR
co Ct
< 35 la
duong tinh Coi kit qud dm tinh virus huyet co gid tri Ct =
40)
Bieu do 3.
Mux:
do virus huyet
- Trudc khi cdng cudng doc (ngdy thir 29),
khdng phdt hien thdy virus PRRS trong
huylt
thanh tdt cd lpn d cac 16.

- C6ng cudng dpe d thdi dilm ngdy
thii
32.
Sau dd lay mdu vao cdc ngdy
thii
35,
39,42,
46
va 55 de kiem tra
mire
dp virus huylt.
Kit
qud
the hien tren bang va bieu do cho thay virus xudt
hien trong huyet thanh lpn dat cao nhdt vdo giai
doan ngay thu 35-42
(tiic
la ngay 3-10 sau khi
gdy nhilm) va giam dan d nhung ngay tiep theo.
- Tai ciing thdi dilm, lupng virus huyet cr
Ion thudc cdc Id ddi chiing cao han Ipn d cdc
16
vacxin tli 4-8 ldn (chenh lech 2-3 Ct).
- Den ngdy
thii
55 khi ket thiic thi
nghi?m,
vdn cdn 1 lupng nhd virus phat hien dupc trong
huyet thanh
Ign

ddi chiing duac c6ng virus
VN07196. Cac nhom khdc khdng cdn virus
trong huyet thanh.
KHOA HOC KY THUAT THU Y TAP XXI
S6
1
-
2014
3.2.3. Ddp
umg miSn
djch (Xem bdng 2 vd 3)
Bang 2. Ket qua kiem tra hieu gia khang the blng
phu'tyng
phap ELISA
Lo
2
4
Virus
CCD
PRRS
21
PRRS
VN 07196
S6tai
2
4
5
13
9
10

11
12
1
3
18
19
8
14
15
Ngay
0
S/P
0,041943
0,335541
0,311258
0,280353
0,19426
0,328918
0,00662
0,015453
0,07064
0,00662
0,01987
0,011038
0,03532
0,04636
0,02649
0,00662
KQ
Neg

Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Ngay 29
S/P
2,27807
0,0241
3,20321
3,59626
0,0214
0
0,03476
0,00802
2,63636
0,0187
2,29412
2,10695
0,03476

0,0053
0,04278
0,05348
KQ
Pos
Neg
Pos
Pos
Neg
Neg
Neg
Neg
Pos
Neg
Pos
Pos
Neg
Neg
Neg
Neg
Ngay 55
S/P
2,3246
2,09607
2,538574
2,11936
1,41048
1,918486
KQ
Pos

Pos
Pos
Pos
Pos
Pos
LON
CH£T
1,606987
2,010189
1,416303
2,525473
1,570597
Pos
Pos
Pos
Pos
Pos
LON
CHET
1,770015
1,390102
1,528384
Pos
Pos
Pos
Bdng 3. Ket qua kiem tra hieu gia khang the bang
phuxyng
phap IPMA
Lo
1

4
Vims cong
PRRS
21
PRI^S
VN 07196
So tai
2
4
5
13
9
10
11
12
1
3
18
19
8
14
15
16
NgayO
Neg
Neg
Neg
Neg
Neg
Neg

Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Neg
Ngay 29
640
Neg
1/2560
1/5120
Neg
Neg
Neg
Neg
1/1280
Neg
>1/640
1/640
Neg
Neg
Neg
Neg
Ngay 55
1/1280
1/640

1/1280
1/640
1/320
1/640
LON
CHET
1/640
1/640
1/320
1/1280
1/320
LgN CH^T
1/640
1/320
1/320
KHOA HOC
KY THUAT
THU
Y TAP XXI SO 1
- 2014
Nhdn xet:
- Trudc khi tiem vacxin vdo ngay 0, tat cd
16
Ion ciia
4
Id
deu dm tinh vai khang the khang
virus PRRS (ca 2 phuang phdp xet nghiem
ELISA va
IPMA)

- Sau khi tiem vacxin Amervac cho 2
Id
dupc 29 ngdy thay rang cd 6/8 con cd khang
thi
khdng virus PRRS. Ty le S/P trung binh vd
hieu gia khang the trung binh
16
1 cao hon
16
3.
Khdng phdt hien dupc khang the khang virus
PRRS tren Ion cua 2
Id
khdng tiem vacxin
{
am
tinh).
Dieu nay
chung
td vacxin khong truyln
ngang tir
16
thi nghiem sang
16
doi
chirng.
- Sau khi c6ng cudng doc 23 ngay
(tire
la
ngay thir 55) cd 4 16 deu cd khang the d 14 lpn

sdng sdt (mdi 16 d6i chiing chet 1/4 lpn). Ty
1^
S/P trung binh vd
hi$u
gia khang the trung binh
d 2
Id
tiem vacxin cao hon 2
Id
ddi chiing Co
the nhdn thdy su chuyen
biSn
ddp
iing
miln dich
tao khdng the thdng qua cac ket qua ciia phuang
phdp ELISA va IPMA sau khi tiem vacxin va
sau khi cdng cudng ddc.
Dilu nay chung to
vi|c sir
dung vacxin
da
kich
thich hinh thdnh dap
ung
miln dich
ddc
hi§u.
Ddp
iing

miln dich dupc thiic day va
ham lupng khdng the tdng cao
6
lpn dupc tiem
vacxin nhd phdn
iing
hdi
lic
sau khi lpn dupc
cdng cudng doc.
3.2.4, Tdng trong (Xem bdng 4).
Bang 4. Theo doi trong
lyvng
trung binh cua
\tyt\
4 Id thi
nghiem
L6
Lo 1
(VX)
Lo 2 (DC)
L6 3 (VX)
Lo 4 (DC)
Ttieo
doi
trijng lu'o'ng
TB
(Itg)
NgayO
6,1

5,9
6,3
6,2
Ngay 32
(CCB)
14,4
14,3
14,5
14,6
Ngay 39
16,5
14,8
16,9
15,2
Ngay 46
19,2
16,1
19,8
16,6
Ngiy 55
22,8
19,0
23,5
19,6
-
O
giai doan sau khi
tiem
vacxin (ngay 0
den ngdy 32),

miic
do tang trpng
Ipm
la tuong
duong nhau giira cdc 16. Dieu nay cho thdy
vacxin kh6ng dnh hudng tdi qua trinh tang trpng
d nhiing lpn dupc tiem.
- Sau khi cdng cudng ddc (ngay
thii
32 trd
dl),
miic
dp tang trpng giam hon so vdi tnrdc .
Sau 23 ngay cdng virus PRRS 21, Id
1
tang 8,4
kg,
16
2 tang 4,7 kg, nhu vay
16
dung vacxin
tang trpng cao ban
Id
ddi chimg la 3,7 kg.
- Sau 23 ngdy cong virus PRRS VN 07196
16 3 tang 9 kg, cdn Id 4 tang 4,9 kg, nhu
v?y 16
dung vacxin tang trpng cao hon
16
d6i chimg la

4,1
kg.
L6
dung vacxin AMERVAC PRRS
tSng
trong tdt
h<ra
so vdi
15
khong dung vacxin khi
cong virus PRRS
cu-dng
dOc.
Dilu
dd
chung
td vacxin bao hp
Ign Qaqic
tiem trdnh nhirng
tac hai cua virus PRRS
cu-dng
dpe.
KHOA HOC KY THUAT THU Y
TAP
XXI SO 1
-
2014
3.2.5.
Bfnh
tich

dtfi
the vd vi thi
&ph6i
Virus
PRRS 21
Virus
VN07196
3ang
5.
Cham
di^m
b^nh
tich phoi khi
Ld 1 - vacxin
So
2
3+
864
3+
So
5
It
So 13
3*
Diem
trung
binii
io = 2,50
Lo 3 - vacxin
S61

2+
So
3
2+
So
18
2+
So
19
1 +
Diem trung
binii
io = 1,75
mo Itham
Lo 2 - doi
chijng
So
9
3->
So 10
2+
s6ii
3+
S6l2
2+
Diem
tnjng
binh io = 2,50
Lo 4 - d6i
clii>ng

S6
8
2+
S6l4
1 +
S6l5
3+
S6l6
2+
Diem trung binh
16
= 2,00
Nhdn xet benh tich dai the:
Sau khi cong cudng doc,
Ion ciia
tdt cd cdc
16 thi nghiem va ddi chiing deu cd cac benh
tich
d?i
the viem d phdi.
B6nh
tich chu yeu
Id
hien tupng viem ke, xuat huyet va khi thung,
xay ra d tat ca cac thiiy ph6i. Mpt sd Ion ddi
chung cd hien tupng teo thiiy phdi (nhdt
Id
thiiy tim). Cac hach
khi
phe qudn bj viem, sung

vd xudt huyet, xudt hien ca d
Id
vacxin va
Id
ddi chiing. Benh tich dai
th€ o
phdi ciia Ign
Id tiem vacxin cd bieu hien viem
nhg hon
so
v^
Ign cua
Id
doi chirng.
Ld 2 - Doi
chLPng
cong PRRS 21
PhSl viem
ke,
xuit
huyil Hach
khi
phe quan
sung,
xuit
huySt
Lo
1
-Vacxin cong PRRS 21
1

Phdi xuk huyet nh? Hach mitig treo ruQt binh thucmg
KHOA HOC
KY
THUAT
THO Y TAP
XXI
SO 1
- 2014
L6 4 -
Ooi
chirng cong VN 07196
Phoi
viem
ke,
xuat
huyet
Hach khi phe quan
sung,
phii
Lo 3 - Vacxin cong VN 07196
I"*
Qj
Hach mang treo rudt binh thuung
Nhdn xet benh tich vi the:
- Benh
tich
vi the cac
16
doi chiing: Ld 2
(c6ng vims PRRS

21)
vd
Id
4
(cdng
vims PRRS
VN
07196)
cd benh tich nang
phii
ne mang phdi,
vdch ngan gian rpng, Idng phe quan chiia dich
ri viem va tl bdo viem, hinh thanh huyet
khdi,
viem mach tang sinh, vach
phi
nang day tang
sinh te bao
phi
nang loai 2. Lo 2 cd bieu hien
benh tich ndng ne
hem 16
4.
- Benh tich vi the cdc
16
vx: L6
1 (cdng
virus
PRRS
21)

va
16
3 (cdng virus PRRS - VN
07196)
cdu tnic phdi cac
diiiy
vdn cdn nguyen
ven,
mang
phdi, vach ngan ro rdng, vdch phe nang mong,
cau tnic
tiii
khi rd rang.
Nhu
vdy,
khi cdng virus cudng doc
PRRS,
16
dupc tiem phong vacxin Amervac cd tac dung
bao ve phdi va cac ca quan khdc nhu
lach,
h?ch,
amidan khdng bi virus tdn cdng, con Id khong
tiem phdng, phoi vd cdc ca quan ndi tang deu co
biln ddi benh tich vi
thi
ndng do su xdm nhap
cua virus PRRS.
KHOA HOC KY THUAT THU Y TAP XXI SO 1 - 2014
Mpt so hinh anh benh tich vi

thg;
Lo 2
B/C
(cdng virus PRRS 21)
:~'-i5.^
V<;;
\
%,
Phdi: Phe quan
chCra
dich viem, bong troc
te bao biiu mo; vach
ph§
nang day, tham
nhiSm
cac
tS
bao viem
Hach khi phe quan: Nang lympho gian
rong, co nhidu t4 bao lympho dang hoat
dong va dai thirc bao
Lo 1 VX (cong virus PRRS 21)
Phdi: Vach ngan day, c6 nhiSu te bao
viem xam nh$p: vach phe nang day
Hach khi phe quan: Nang lympho gian
rong, tang sinh dai thirc bao
Lo 4
Die
(cong virus PRRS -VN 07196)
Ph6i:

Vdch
phi nang day, mach quin hinh
thdnh
huyet khoi
;^3^%;:^^?»s;ra.ipi^%f.
H$ch
khi phi quan: Nang lympho gian
rQng,
tang sinh
dai thijc
bao va co ti bao
lympho hoat dgng
KHOA HOC
KT THUAT THI? Y TAP XXI S6 1 - 2014
L6 3 VX (cong virus PRRS -VN
07196)
Phoi: Binh
thuxyng
IV.
KET LUAN
Dua vao cac ket qua thuc nghiem neu tren,
CO
the riit ra cdc ket luan nhu sau :
- Vacxin nhuac doc AMERVAC PRRS do
hang Laboratorios HIPRA, S.A san xuat dam
bao an todn khi sir dung tiem phdng cho lan.
- Virus vacxin khdng truyen ngang tir 16 thi
nghiem sang
Id ddi
chimg va cd tdc dung kich

thich sinh dap ung mien dich khi Ian bi nhiem
virus cudng ddc (hodc c6ng cudng doc) dap ung
mien dich se manh ban.
- Dap ling mien dich do vacxin tao ra cd tac
dung 1dm giam
miic
dp tram trpng cua benh
thdng qua kiem tra benh tich dai the vd vi the
tren cac ca quan npi tang, dac biet la ph6i cua
lpn sau khi bi nhiem virus cudng doc PRRS.
- Lo diing vacxin AMERVAC PRRS tang
trpng ban so vdi
Id
khdng dimg vacxin khi cdng
virus PRRS cuong ddc.
- Dap ling mien dich co dupc nhd vacxin
da loai bd hoan todn virus trong ca the Ipn vao
khodng 2-3 tudn sau khi bi nhilm. Lpn
dugc
tiem vacxin, khi bi nhiem virut cudng doc se
khdng dao thai virus cudng ddc ra mdi
tnidng
xung quanh.
TAI
LIEU
THAM KHAO
1.
Tian et al. 2007.
PloS
ONE 2: e526.

2.
Li et al. 2007.
Vet
J.
174: 577-584.
3.
Nam et al. 2009. Arch
Viroi
154: 629-638.
4.
Amonsinetal. 2009.
f77-o/y.
16: 143.
5.
RocaM.
etal.
2012.
Vet
J.
193: 92-96
Nhan
ngay 3.10.2013
Phdn
bien
3.11.2013