Nghiên cứu ảnh hưởng của một số yếu tố kỹ thuật sử dụng tinh trùng đã xác định tính biệt cái đến khả năng sản xuất phôi in vitro và in vivo trên bò Holstein Friesian (Luận văn thạc sĩ)

IH
IH

------------------------------

NGUY N TH TUY T NHUNG

U
Y U T K THU T S D
N KH

NG C A M T S
NH
N XU
HOLSTEIN FRIESIAN

LU
KHOA H

P

- 2015


IH
IH

--------------------------------

NGUY N TH TUY T NHUNG

U
Y U T K THU T S D
N KH

NG C A M T S
NH
N XU

: 60.62.01.05

LU

C
P

ng d n khoa h c:
m

2. TS.

- 2015


i


ii

Sinh

iii

............................................................................................. i
.................................................................................................. ii
....................................................................................................... iii
............................................................... vi
...................................................................................... vii
............................................................................viii
.......................................................................................................... 1
.

........................................................ 3

1.1. B

........................................................................................... 3

1.1.1.

........................................................................................3
............................................................................4

1.1.3.

..................................................................................5
............................................................................................. 6

1.2.1.

...........................................................................6
.............................................................................7

1.

.......................................................................................... 8

1.3.1.

,

..........................................................8
.........................................................................................9
.......10

................................................................................................. 12
..................................................12
...........................................13
1.4.3
........................................................................16
............................................................................... 17
........................................................................................18


iv

...........................................................................................20
.............................21
....................................................................................22
................................................................................ 24

1

.............................................. 25
.

............ 27
............................................................ 27
....................................................................................27
.......................................................................................27
................................................................................ 27

itro. .........................27

..........................................28

..................................28

..........................28
.......................................................................... 29
..............................................................29
..............................................................32
.............................................................................34
.

............................................... 35

............................................................. 35
3.1.1.
3.1.2. Ch

..............................................................................................35
.................................................................................37


v

............................................................................................39
.....................................41

....................................................... 45
...............................................45
...................................................................................47
..............................................................................48

.............................................................. 50
...............................................51
3.3.2.

...................................................................................53
..............................................................................53

.............................................................. 55
..........................................................................56
...................................................................................57
3.4.3.

..............................................................................58
........................................................................... 61
............................................................................ 62
....................................................................................................... 68

vi

BSA

: Bovine Serum Albumin

FCS

: Foetal Calf serum

FSH

: Follicle Stimulating Hormone

GnRH

: Gonadotropin Releasing Hormone

HEPES

: N-(2 Hydroxyethyl) piperazine-

HF

: Holstein Friesian

LH

: Luteinizing Hormone

LTH

: Luteo Tropic Hormone

PRH

: Prolactin Releasing Hormone

MEM

: Minimum Essential Medium

PGF

: Prostaglandin F

SOF

: Synthetic oviduct fluid

TCM-199

: Tisue culture medium 199

PCR

: Polymerase chain reaction

NST

:

-(2-ethanesulforic acid)


vii

3.1:

c ...................................................... 35
...................... 37
.................................................... 39

................................................................................... 42

........................................................................................ 45
...................................... 47
c
............................................................................. 49

........................................................................................ 51

............................................................ 53

................................................................................ 54
p

...................... 56

................................................................ 57

............................................................................. 59


viii

h 1.1:

..........................................................................3
....... 11
.............................. 17
.................................................... 23
......................................................... 33
............................. 36
..................................................................................... 38
............................................................................. 39

........................................................... 40
................. 42
:

...........................................................................................43

7:
........................................ 44

.............................. 50
:

.........52

3.10:

........................................................................................... 55
.................................................. 57

............ 60


1

1.

,
nhu

C
in

,
vivo,

in vitro

c

. Song,

sinh ra.

,

h
.

,
in


2

vivo

in vitro

in vitro

in vivo.

in vitro
vivo

.

-

in

3

1.1. B
1.1.1.

.
sinh,

(

(

)

).

,

...

0,3g,
10 - 20g.
,

,

,
3

15 mm (

35 x 25 x

., 2000) [9]
Nang tr

Nang tr

Nang tr

Nang tr
(Tertiary)

Nang tr

) [9]

(
2
(

).

:

(

)
,

4

.

,
.

,

(

).
.

.
,

,

,

,
,

,
.

h

,
ngay


5

.
1.1.3.

.

21

17 24).

,

10

(dao

2 pha:

24

,

.

.

10
10 20 th

,

11

12

.

-

.

,1992)
[6]


6

tr

(

1990) [50].

e cao

,

14mm)

, 1989) [18]
,

nang,
,
(

, 2002) [51]. Song,

(

, 1997) [7].

1.2.1.
.

,

,c
.
,
,
(

).

,

111
.

1

.

130

,
1
.


7

2
quanh 1

(nang

).

,
,

. Xoang

y 130

(Bearden va cs,

2000) [9].

.
.
,

.
1

:n

(

),

.
.
hormon estrogen.

,

,

.
.
.

ne
(

.

).

.
:

50%

1.2.2.

80% (

, 2000) [9


8

,1991) [14]

1.3.1.

,
,
,

.

,

3

,

:
:

:
.
(

).

,


9

.
.
100.000

,

.
:

,
(

),

(2n).
:

1,

1

1

.

1

(

)

(

).
.
1

;

:
.

,

,

i


10

Nguy

1.3.3
n

-

-

e Stimulating Hormon (FSH),

-1 (Insulin liked
Growth Factor-


11

(

, 1995) [12].

Prolactin Releasing Hormone (PRH)

Progesterone. Progesterone

e do th

progesterone

,

,

,


12

progesterone

1.4
1.4

DNA

n

120

Tinh


13

1.4

-

)

,

tin

DNA

Williams (1986) [54],

-6-photphate


14

Theo
(1988) [37],
phosphoribosyl transferase.

(1971) [19],

,
(1985) [39],

chia

Theo Schrod
cs (1990) [45],

A

s


15

,

(

, 2004) [52]
,

. Theo Seidel
(1999) [47]

nh sau khi

(

, 1999) [43]

in vitro
in vitro
1999) [35]

(2004) [34], Lu
(2006) [55]

(2003) [56]

in vitro
uy
nhi , theo

(2006) [36]

,

, 2006) [55]; (
[38]
tinh tr

, 2005)