Tạo tôm càng xanh toàn đực Macrobrachium rosenbergii nhờ bất hoạt gen insulin like tuyến đực qua công nghệ can thiệp RNA

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VIỆN NGHIÊN CỨU NUÔI TRỒNG THỦY SẢN 2

ALL MALE REPRODUCTION OF Macrobrachium rosenbergii BY INSULINLIKE GENE SILENCING IN THE ANDROGENIC GLAND THROUGH RNA
INTERFERENCE
Bui Thi Lien Ha1, Nguyen Duc Minh1, Le Thi Hoai Oanh1, Tran Thanh Vo1, Nguyen Dien1,
Le Chinh1, Nguyen Viet Dung2, Nguyen Van Hao3

ABSTRACT
Within the aquaculture industry in Vietnam, the demand of the all male prawn Macrobrachium
rosenbergii seed is one of overriding importance.  Macrobrachium rosenbergii is an important
freshwater prawn species having commercial importance in Vietnam. The all male prawn farming
brings most highly profitable for farmers; therefore seed demand for popular culture is always
very high. Lasted technology called RNA interference for creating neo-female is inactivated a
single IAG-encoding gene of male insulin-like prawn (Macrobrachium rosenbergii insulin-like
androgeneic gland, Mr-IAG) for producing all male seed is being successfully implemented at the
Research Institute for Aquaculture No. 2. The currently result has achieved with double-stranded
RNA (dsRNA) synthesis to mRNA encodes Androgenic Gland. The important factor for success is
very young shrimp age at the time of the first injection, period and time of injections. Sex reversal
rate after two to three months injection is 88-92% female, dsRNA of injection time around 2 months;
frequency of dsRNA injection is 2 weeks.
Keywords: fresh water prawn, sex-reversal, iRNA.

Người phản biện: ThS. Nguyễn Thị Hiền

Ngày nhận bài: 10/02/2014
Ngày thông qua phản biện: 28/02/2014
Ngày duyệt đăng: 01/4/2014

Department of Experimental Biology, Research Institute for Aquaculture No 2.
Email:
2
Southern Monitoring Center for Aquaculture Environment and Epidemic, Research Institute for Aquaculture No 2.
3
Research Institute for Aquaculture No 2.
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