Vài điều về CrisprCas9 (tiếng anh)

CRISPR-CAS9


01.

What is CRISPR/Cas9?

02.

How CRISPR/Cas9 Work?

.

03. Applications of CRISPR/Cas9.

04.

Future of CRISPR/Cas9.


History of CRISSPR/Cas9

The CRISPR defense system is

CRISPR was called "breakthrough"

first identified in prokaryotes.

.
scientific research.

2000
CRISPRs were described Japanese
researchers.

2020

2013

1980s

2015
The CRISPR/Cas system can be used
.

for mammalian cell.

CRISPR method has been awarded the
Nobel Prize.


What is CRISPR/Cas9?
“ Molecular scissors ”


Components of the CRISPR/Cas9 System

Cas9

-

gRNA
An enzyme
The primary function is cleaving.
A nuclease which targets DNA.

gRNA
- gRNA = crRNA + tracrRNA

Cas9

- ‘guides’ Cas9 to the right part of the genome.


The CRISPR/Cas9 editing tool works.

gRNA finds the spots and guide the Cas9 to a target.

1

2

Cas9 cuts the target DNA.

3

The cut is repaired.


Diagram showing how the CRISPR-Cas9 editing tool works.

Image credit: Genome Research Limited.


Comparison of CRISPR/Cas9 with other Genome Engineering
Technologies

Zinc Finger Nuclease (ZFN)

04

Transcription Activator-like
effector-based nuclease (TALEN)

05

How CRISPR is different from
other gene editing tools?

Meganuclease
06


Advantage
Need attention?

of the method

-unintended

Accurate, efficient,

mutations

fast

and cheapest.
- Ethical concern

Can change the specificity.

 Can target

multiples genes.


Applications of CRISPR/Cas9

CRISPR Use in Agricultural Sector

Use of CRISPR in Cancer

CRISPR/Cas9 for
Gene-editing

CRISPR as Antibiotic Resistance Solution

CRISPR/Cas9 has the
potential and
can be used in various fields,
from Medicines to agriculture

in the controlled environment.

CRISPR for AIDS

CRISPR Gene Drive


What’s the future of CRISPR-Cas9?
There are several problems associated with CRISPR/Cas9, like ethical issues and off-target mutations.
Once these issues are solved, CRISPR/Cas9 will genuinely become a revolutionary medication.


Reference

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Thank You!