Tạo khối cầu đa bào ung thư biểu mô tuyến vú dòng MCF-7 tt

Tạo khối cầu đa bào ung thư biểu mô tuyến
vú dòng MCF-7

Nguyễn Đắc Tú

Trường Đại học Khoa học Tự nhiên
Luận văn Thạc sĩ ngành: Sinh học; Mã số: 60 42 01 14
Người hướng dẫn: TS. Hoàng Thị Mỹ Nhung
Năm bảo vệ: 2014


Abstract. Tạo khối cầu đa bào ung thư từ dòng tế bào ung thư biểu mô tuyến vú
MCF-7. Nghiên cứu các đặc điểm về hình thái, cấu trúc, động học tăng trưởng của
các khối cầu đa bào ung thư MCF-7 đã gây tạo. Sử dụng mô hình khối cầu đa bào
ung thư MCF-7 để kiểm tra độc tính của hệ thuốc nano (Ptx-Cur)-Co. Nghiên cứu sự
xâm nhập và phân bố của hệ thuốc (Ptx-Cur)-Co vào bên trong khối cầu đa bào
ung thư MCF-7.

Keywords. Sinh học thực nghiệm; Tạo khối cầu; Ung thư biểu mô; Tuyến vú; Đa
bào ung thư

Content

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MỤC LỤC
DANH MỤC HÌNH
DANH MỤC BẢNG
DANH MỤC CHỮ VIẾT TẮT
MỞ ĐẦU 1
1.TỔNG QUAN 3
1.1.Sàng lọc thuốc 3
1.1.1.Quy trình sàng lọc thuốc 3
1.1.2.Các mô hình sàng lọc thuốc tiền lâm sàng 5
1.2.Mô hình nuôi cấy khối cầu đa bào ung thư 11
1.2.1.Nuôi cấy ba chiều (3D) 11
1.2.2.Mô hình nuôi cấy khối cầu đa bào ung thư 13
1.2.3.Các vấn đề kĩ thuật liên quan tới nuôi cấy khối cầu đa bào 19

2.ĐỐI TƯỢNG VÀ PHƯƠNG PHÁP NGHIÊN CỨU 27
2.1.Đối tượng nghiên cứu 27
2.1.1.Dòng tế bào ung thư vú MCF-7 27
2.1.2.Hệ thuốc nano 28
2.1.3.Máy móc thiết bị và vật tư tiêu hao 28
2.2.Phương pháp nghiên cứu 31
2.2.1.Hoạt hóa và nhân nuôi tế bào 31
2.2.2.Gây tạo khối cầu đa bào ung thư 31
2.2.3.Nghiên cứu động học sinh trưởng của khối cầu đa bào ung thư 33
2.2.4.Thử độc tính 34
2.2.5.Nghiên cứu khả năng xâm nhập của thuốc vào mô hình khối cầu đa bào 39
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3.KẾT QUẢ 40
3.1.Hoạt hóa và nhân nuôi dòng tế bào ung thư vú MCF-7 40
3.2.Gây tạo và nghiên cứu động học sinh trưởng của khối cầu đa bào ung thư MCF-7 41
3.2.1.Kết quả tạo khối cầu đa bào ung thư MCF-7 41
3.2.2.Động học và một số đặc điểm sinh trưởng của khối cầu đa bào ung thư MCF-7
45
3.3.Thử nghiệm độc tính của ((Ptx-Cur)-Co 51
3.3.1.Độc tính trên mô hình tế bào MCF-7 nuôi cấy đơn lớp 51
3.3.2.Độc tính trên mô hình khối cầu đa bào ung thư MCF-7 54
3.4.Sự xâm nhập của thuốc vào bên trong khối cầu đa bào ung thư MCF-7 66
4.BÀN LUẬN 69
5.KẾT LUẬN VÀ KIẾN NGHỊ 75
5.1.Kết luận 75

5.2.Kiến nghị 75
TÀI LIỆU THAM KHẢO 76








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