Nghiên cứu tác dụng hạ đường huyết và khả năng chống rối loạn trao đổi lipid của dịch chiết từ loài vú sữa đất (euphorbia hirta l ) trên mô hình chuột đái tháo đường thực nghiệm

I




KHOA SINH  KTNN
*****************









LIPI
(Euphorbia hirta L.) TRÊN MÔ HÌNH











- 2014
II



 
cô giáo ThS. và  

Em xin chân thành 
môn Hoá Sinh, Khoa Sinh- 
 .
Em 

Hà Nội, tháng 5 năm 2014
Sinh viên



III






Hà Nội, tháng 5 năm 2014
Sinh viên



IV

MC LC

Li c
L

Mc lc
Danh mc các hình, bi
Danh mc các bng

 1
NG QUAN TÀI LIU 5
1.1. Hp cht t nhiên  thc vt 5
1.1.1. Hp cht phenolic 5
1.1.2. Flavonoid thc vt 7
1.1.3. Tannin thc vt 10
1.1.4. Hp cht coumarin 11
1.1.5. Alkaloid 12
1.1.6. Steroid 13
1.1.7.Terpen thc vt 14
1.2. Bnh béo phì (Obisity) 14
1.2.1. Thc tr gii và Vit Nam. 15
1.2.2. Tác hi ca bnh béo phì 15
1.2.3. Nguyên nhân gây béo phì 16
1.2.4. Ri loi lipid 16
1.3. Bng. 18
1.3.1. Khái nim và phân loi. 18
1.3.2. Thc trng trên th gii và Vit Nam 19
1.3.3. Phân lo bnh sinh c 20
1.3.4. Tác hi và bin chng. 22
V

1.3.5. Mt s thuc tng hu tr b 23
ng vi y hc c truyn (YHCT). 23
1.4. Mi quan h ging. 24
1.5. ng STZ. 25

1.6.Vài nét v cây Vú st (Euphorbia hirta L.) 26
1.6.1. Mô t 26
1.6.2. Phân b, sinh thái 26
CHU 28
ng nghiên cu 28
2.1.1. Mu thc vt. 28
2.1.2. Mng vt 29
2.1.3. Hóa cht và dng c thí nghim. 29
2.2. u 29
t mu nghiên cu. 29
o sát thành phn hóa hc ca cây Vú st
(Euphorbia hirta L.) 30
2.2.4. Sc ký lp mng 33
2.2.5. Nghiên cu tác dng h ng huyt ca dch chit cây Vú sa
t (Euphorbia hirta L.) lên chut nhng STZ 33
2.2.6. S d- c 35
2.2.7 . X lý s liu. 37
KT QU VÀ THO LUN 39
3.1. Kt qu tách chit và mt s c tính hoá sinh cn dch
chit t cây Vú st (Euphorbia hirta L.) 39
     n dch chit t cây Vú S t
(Euphorbia hirta L.) 39
3.1.2. Kt qu nh tính mt s nhóm hp cht t nhiên trong các phân
n dch chit t cây Vú st (Euphorbia hirta L.) 40
VI

3.1.3. Phân tích thành phn các hp cht t n
dch chit cây Vú st (Euphorbia hirta L.) bng sc kí lp mng 41
ng polyphenol tng s trong cao dch chit
n t cây Vú st (Euphorbia hirta L.) 42

3.4. Tác dng h glucose huyt ca mt s n dch chit t cây
Vú st trên mô hình chu 51
3.4.1. Kt qu to mô hình chuc nghim 51
3.4.2.Tác dng ca mt s n dch chit t cây Vú st n
n glucose huya chu 53
3.5. Tác dn chuyn hóa lipid ca cây Vú s t trên mô hình
chu 56
KT LUN 59
KIN NGH 60
TÀI LIU THAM KHO 61

VII


 7
Hình 2.1. Cây (Euphorbia hirta L.) 28
Hình 2. 29
Hình 2.3.  36
 39
 41
 acid 43

Hình 3.5
 46
Hình 3.6. 
.
48
Hình 3.7       
 53
Hình 3.8. 

 55
Hình 3.9.  
-Hexan và cao phân
 58

VIII


Bảng 1.1. Phân lo BMI của người trưởng thành châu Âu và châu Á 14
 30

(Euphorbia hirta L.) 35

 39

(Euphorbia hirta L.) 40
 43
H 
 44
 . 44

 46

 48

STZ 51

 54

-

EtOAc 57

IX


BP
Béo phì
Cho
Cholesterol


EtOAc
Ethylacetat
EtOH
Ethanol
Glu
Glucose
HDL
-density lipoprotein)
LDL
-density lipoprotein)
n-hex
n-hexan


STZ
Streptozotocin
TC

TG

Triglycerid
















1

M U
1. Lý do ch tài.
 
 
 



           
 
, 

 


 

n nay


             
sulphonylurea, metformin, glitazone, in      



V



2


C




- 
 

- 
[4], [9], [10], [12].



 
       (Euphorbia hirta L.)   
 (Euphorbiaceae) là m
Vú 
         


 

các loài C




3

Tuy nhiên v
              
:
i
 (Euphorbia hirta L.,) trên mô hình


- 
cây  (Euphorbia hirta L.)
-  
  
3. Nhim v nghiên cu

- 
t (Euphorbia hirta L.)
- 
- 

(Euphorbia hirta L.)
ng và phm vi nghiên cu

4.1.1 
+ V(Euphorbia hirta L.)

Xuân Hoà  Phúc Yên  
4.1.2 
+   14-

4.2. 

4


 (Euphorbia hirta L.) m,


5




1.1. Hp cht t nhiên  thc vt
           




phenolic, nhóm terpen và nhóm alkaloid.
1.1.1. Hp cht phenolic

C
   -        

 [21].
1.1.1.2. 



 

        

 Tr
       
6
      

 
 

6








Acetyl-
- 

- 


-      
-oxydase,


-  


nguyên -  



 
- 
- 
-  


7




 
1.1.2. Flavonoid thc vt
1.1.2.1. 



6
 C
3
 C
6
.

Hình 1.1. Khung cacbon ca flavonoid




phâ    

- glycoside và C -
- onoid thông
       -    



8




-phenylbanzopyral) (A), isoflavonoid (3-
banzopyran) (B) và neoflavonoid (4-benzopyran) (C).
              
 
2
và C
3

4




catechin.



 

15
-coumanyl
CoA), flavanon, flavan-3,4-

            






- Phn ng c-OH): Phn ng vi các ch
persunfat, ferixianit, các gc t o thành gc phenoxyl ArO
*
là gc
t do b.

9

- Flavonoid có tính acid nên d dàng phn ng vi dung dch kim (Ví d
dung dch amoniac) to thành các loi mu

- Phn ng c 
gia phn ng diazo cho sn ph, da cam.
- Phn ng ca nhóm cacbonyl: Phn ng Shinoda to phc kim lo
phn ng kh, có s tham gia ca các kim lo
cho sn phm màu da cam.

4

C
4
và C
3
-3. Flavon không có



-   
kìm 




- O
2

polyphenoloxydase


- H
2
O
2
+ Flavonoid (dng kh)
Peroxydaxe
Flavonoid (dng oxy hóa)
 
2
O


nh ra trong quá


10

- Flavonoid có kh u hoà ho enzyme do kh t vi
nhóm amin trong phân t i cu hình không gian ca
o hiu u hoà d lp th,
- Flavonoid có tính kháng khu  kháng ca
        n xut interferon, c ch hin

ng thoát bng (dirgramilation).
- Flavonoid có hot tính ci ca
thành mch, gim sc thm ca mao mch.
- Flavonoid có tác dng ch
khng phân, hô hp, kìm hãm phân bào phá v cân bng
i cht ca t 
- Flavonoid có hot tính ch






1.1.3. Tannin thc vt
Tannin (tannin, tannoid
     polyphenolic          
 protein  axit amin và
alkaloid.



 .

11

 
               

 
 

           
               
Tannin có            ( axit
galic este          
  , gelatin   
 .



Gallic axid

Flavone

Phloroglucinol
.

-
6
- C
3





Coumarin

12







1.1.5. Alkaloid


             
 


N
N
N
N
C H
3
C H
3
CH
3
O
O
C a ff e in





  -         

              c


            
Tinosporacordifolia, Coptis
sinensis), Casuarine 6-O-- glucoside (Syzygium malaccense).
O
N H
H
OH
OH
M o rp hin e
N
N
C H
3
N ico tin

13

1.1.6. Steroid
Steroid 
 cycloalkane 
   cholesterol       
dexamethasone.
     





cacbon 




    sterol          
nhóm hydroxyl  cholestane. 
 ,  và 
         lanosterol     
 cycloartenol         
 triterpene squalene.


14

1.1.7. Terpen thc vt


5
H
8
), có công

5
H
8
)
n



3-
               

1.2. Bnh béo phì (Obesity)

 

BMI (Body Mass In


2
W
B M I
H




Bng 1.1. Phân loi BMI cng thành châu Âu và châu Á




<18.5
< 18.5

18.5 - 24.9
18.5 - 22.9

- 29.9



30 - 34.9
>23 - 24.9
BP 
35 - 39.9
25 - 29.9




15

1.2.1. Thc tr gii và Vit Nam.
* Trên th gii:




  
                

t Nam:
a cân BP

 


            
-     


 
 -



- 


1.2.2. Tác hi ca bnh béo phì



16





  [14].
1.2.3. Nguyên nhân gây béo phì


- Khu phng
- Hong th lc kém.
- Yu t 
-Yu t kinh t xã hi.
1.2.4. Ri loi lipid
1.2.4.1. Khái nim


    
   



lipid máu.
1.2.4





