BÁO CÁO THỰC HÀNH MỘT SỐ PHẢN ỨNG SINH HÓA



MỘT SỐ PHẢN ỨNG SINH HÓA
1. KHẢ NĂNG CHỊU MẶN
•  !"#$%&%$'()
• *+%(, /0/ (,%1(20#,2#34)56
• 78(9 /:$%&%$'(; !&<(=(>%(,?+%(, @
-A3>%&<8()
2. THỬ NGHIỆM BẰNG UREASE
NGUYÊN TẮC
• B"#$%&%$'((CAD1EF1 ,1#&1G(&H("F
DI ,1#(>?,#J FK(L
M
$N
J
3N(D"#+%(, $N;
(O-A(1?PQ%9 #&H(#FC%N "#R(S(D)
• L%T ,1#&13N!(,-?3?N%U,?(1 &&DD)N( -A
PV  O  DI  W%T(    P> U,?(1 & $X%    (N  $%K    "#
Y(1,?W#(1,%#1#1)
• Z&[&%;#/\
J
]
J
2
J
^J
M
2
J
^(D+%(, ^_

D1?3)
MÔI TRƯỜNG
L%T`,1#&1-A(H%T(,KJ+%(, /
• *+%(,  ,1#3_a &(%%#b( #,(c&`,1#&1,?(
• *+%(, ,%&(1&1`,1#\+%(, (>%K]
KẾT QUẢ:
*+%(,  ,1#3_a &(%%#b( #,(c&`,1#&1,?(/
• d-Z(e\2]/*N _(e(,?fD+%(, 
• g(e\b]/*+%(, +;&HC%N \N $N#]
*+%(, ,%&(1&1`,1#/
d-Z(e\2]/*N _(e(,KWh!(+%(, (>%K
• 2222/(?NWi(>C%N 
• 222/S!((>C%N 
• 2/!((>[SC%N @Dj+%(, k3>%+C%N 
1
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG

\2]#/N C%(,?$klbm%.
\2]'/N C%(,?$kJn%.8mNF"?!3I Z)
3. THỬ NGHIỆM GELATINASE
MỤC ĐÍCH
L%TDI%%13#(%W[%13#(%#&1
CƠ SỞ SINH HÓA
13#(%1 D?3FD1D(%P12#%P#%
13#(%1(,?+%(, P%P-o^+%(, +!
DI"F13#(%1^+%(, 3_)
 p%qP-Z/01,??#&FP,?D%3#
 p%qI/Y)?3%
 Môi trường sử dụng/ (,%1(13#(%1
 d>p%T(>&I 

 RF$%&%$'($N"[%T(iDk)
KẾT QUẢ:
 r8(9 
\2]/+%(, (#F
\b]/+%(, +(#F
4. THỬ NGHIỆM AMYLASE
MỤC ĐÍCH
U(%T$%&%$'(;T1EF1#F3#&1
dV( p(L
J
O'W%8()8 (,?+%(, (L%T@$%&%$'(;T
1EF1#F3#&1@#F3#&1&<("FDI(%Wi((N:j (L_Z@%
?
J
$N?@P P;N (e#\2])
5. THỬ NGHIỆM KIA/TSI
2
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG
gelanase

MỤC ĐÍCH
U(%T/
 &LPs =#W?FP,#(1
 &%
J

 (>?Z%\#&]
MÔI TRƯỜNG: KIA: Kligler iron agar
 U1D(? Jt
 u#(?&1 Jt

 3 ?&1 l
 #3 5
 v1,%#?% %(,#(1 t@5
 ?P% (%?& 3D#(1 t@5
 0#, l5
 U1?3,1P t@tJ5
 -XR( l3e(
Dw@nxt@J
• *+%(, 70S;#%3?>% t)l63 ?&1@l63#(?&1)
• *+%(, ;(NDj%p70@;WC& (Kl6& ,?&1)
KẾT QUẢ:
3
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG
Quan sát: Phần nghiêng /
phần sâu / hơi / H
2
S
37
0
C/24h

Z8/;W#(, ADF,#/
• S&LPs3 ?&1@&# l4bJn +%RFDj%K\Wh!(](,[K;
D%h$NDjq\Dj&I (,?p%T];D#%P)d?3 ?&1
(,KWh!("#+%(, -A$%&%$'(?F;#?N(?N(N
J
$N
-XO( 3RF 3-A@Dq  j  3-A(,?(
(,-[)%&%$'((%8D(sP;#D1D(?19 #;%%D;
M

3NDj
Wh!("#+%(, ;D%h),?%;@[Dj&# (,?+%
(, ;%h %T?F+jF"@3 ?&1-A3K1e&%
#%P: ;3ND+%(, %)
• LPs3 ?&1$Nu#(?&1@&# l4bJn@(?NWi+%(, h (,[K
;U#%P$y&HW%8P-o=(.%#%3?>% %GD$%&%$'("
3-AO((,-[N-#j&LPs8D1D(?1)8 z?PN%
(.%%# +%RF9 JnWh!(D&<(,[K%hP?8( =#W?
$y$%&%$'(DS#&LPs8D1D(?1)
• 7+&LPs3 ?&1@3#(?&1/$%&%$'(&<W%8P-oD1D(?1O( 3RF
3-A$N$'(R(?&H((,-[)( F%KP?D1D(?1S-A
W%8P-o(,?%h %T%8 eK%T(-A%h;#"#+%
(, SP%{,#(,KWh!(+%(, )
7&%
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P?(,?+%(, &?P% (%?& |#(@$%&%$'(L& |#1
;(OLR(NF@.;1EF1(%?& |#(,1P (#&1O%%D;
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Dq$X%?v1
J2
"#S(#?% %(,#(1(>?8(("#N 1v
J
)
4
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG
ĐC
1

2
3
4 5 6 7
8 9
10
11

*i(&p(, AD;(OF,#%PV+%(, 70}
~•#/P#3?>%$% €;/&LPs =#W?
# $N;&%
J
@&%Z%) -APVOP#
$% €,#\b]@$%&% , i()
6. THỬ NGHIỆM KHẢ NĂNG SINH H
2
S
MỤC ĐÍCH: U(%T&%
J
"#$%&%$'()
CƠ SỞ SINH HÓA:
0%P#%q# 
J

%?& 3|#(1 
J


J
&%,#-A'W%8(W[%%?&f(@y (>?8(("#N 1\v1@
UW])

MÔI TRƯỜNG:
• 70@0\(>%K]
• *@U0\(>&I ]
• 0\(>•#]
5
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG
desulfohydrase
Thiosulfate reductase

RF$%&%$'(3K+%(, @•Mw
?
@Jnbn4
KẾT QUẢ:
• B R(%TN 1(,?+%(, \2]
• 7+ R(%TN 1(,?+%(, \b]
7. THỬ NGHIỆM CATALASE
MỤC ĐÍCH : U(%T$%&%$'(;T1EF1#(#3#&1
CƠ SỞ SINH HÓA:
#(#3#&1%TP%T[%8 e$N‚e(VFƒ

J

J
 
J
2
J
\Wr(e]
\FP,?1D1,?%P1]
THỰC HIỆN

◦ 3RF(„+%(,  +%RF\3_@,f]
◦ !(3K3#e&>
◦ _
J

J
Mt6
6
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG
ĐC
Catalase

◦ … #&(&# lbJ%IF
 Uq\2]/;Wr(e R(%T
 Uq\b]/+;Wr(e R(%T
L%T#(#3#&1(,K•#D1(,%/&LPsJJMt6
8. KHẢ NĂNG LÊN MEN CÁC LOẠI ĐƯỜNG
NGUYÊN TẮC:
- B        "#  i(  &p  $%  &%  $'(  3K  1  \DI  %%]  i(
#,W?FP,#(1!%T ;(,?+%(, ZWO((,-[)
- 7%&LPs =#,W?FP,#(1O3K1@(VF(1?D-Z(q3K
1&D€-A(>?,#&<# /,-A @#%P: Z@
J
†
,?(, AD@&D€(>?(Nh 3N%D"#+%
(, )
-  
J
 -A(>?(N&<-AW‡F3>%(>?(NWr(e(,?p +
d ,#3NC%p +NF\(, AD&LPs+%(, 3_]

?!  3N  $o (>%RF(,?+%
(,  (>  &I
\(, 
AD
&L  Ps
+% (, ,f])
%T  % D(O%T9 #&H
(#FC%N "#R( S  (  D  ;  (,?  +%
(, )
7
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG

- L%T3K1( -APVOP#$%&%
$'( , i(\Y(1,?W#(1,%#1#1 ;3K13 ?&1@Y)?3%@
731W&%133#@Y(1,?W#(1,3K1-A3 ??&1$N3#(?&1]$NDIW%T(i(
&p%p$%&%$'()
MÔI TRƯỜNG/U1?3a1P,?(#&1
THỰC HIỆN:
RF$% €X%?>(?$N?p%T@!([Mm
t
@(1?PQ%%T(-A
&%#%P&# lbMNF)
bur$+(,V+%(, 9 #N3r;e(-X3ˆt@n5‰?!t@J‰)
bUIDp%$N?p%T)RD[lJl
t
(,?l5DG(
b%8NRF"$N?p+%(, W9 1RF$k)
b•p+%(, [Mw
t
(,?l4ŠJt%.),?(, AD‹8(

9 I(e@jPVD#,#|Œ%OW(eG(W+$N(1?PQ%(.%%#";(O
z?PN%8(,?lnbMtNF)
KẾT QUẢ:
• Uq\2]/+%(,  FON $N
• Uq\b]/+%(, ;N _
8
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG

9
TRẦN THANH TÙNG – MSSV: 10243221| Thầy: TRƯƠNG VÕ ANH DŨNG