Thu mẫu và ly trích tuyến trùng từ thực vật

se, i 21: Thu mau va ly
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tuyén tring tu thuc
vat


Objectives of sampling and
extracting nematodes

O Identify the nematode
O Population estimation
O Diagnose a nematode disease
CO Make management decisions
- Regulatory purposes
- Research and surveys



Hinh 48. Mot s6 dung cu dé gap và xử lý tuyến trùng.




Materials required
Pan or tray

Wire mesh
Tissue paper, coffee filter

Squeeze bottle
500 mesh sieve


Place jars in tray


Hand: never uproot plants or take soil samples
SoIl sampling tuhe (15-20 cm)
SoIil auger (more than 20 cmn)
Spade or garden trowel (in rocky soil)

Screwdrtver (to scrape the soil from the sampling tule)



Phuong phap thu mau


Fig1
:

«Sampling pattern

- Systematic at equally-spaced point according
to the grid pattern (Fig1)

- At equally-spaced points along a Ìine running
diagonally across a Íield (Hg2)

pa

- Sampple free crops in the drip line,
on alternate sides of the trunks and include

feeder roots (Fig3)

es


For annual crops, collect samples at 10-20 cm trom

the stem, in the root zone.

Collect samples of weeds and volunteer crop plants
Collect sample from: soil, roots, leaves, stems,
buds, flowers, seeds

Number of samples: 10 for 5m? in size or less
fields, 20 for 5m?-100m? fields, 30 for larger fields


«

Sampling depth: 20 cm tor most crops, 20-100
cm for perennial crops according to type and age
of crops, 30-45 cm in regions with hot and dry
sumimers
Timing: prior to planting (relation of population

density with yield), between mid season, at
harvesting


Sampling pattern for damaged area or
infected patch in a crop.

Damaged
Area
-~@-.

BS

® = Soil Sample


Sampling pattern for individual tree or
shrub.
‘
Trunk

® = Soil Sample

Drip
Line


Soil sampling pattern for row crops.
Start

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How

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-,

#

`

e--#

ằ - e

`

`

?

e--đ

s--@

To

C

ằ--
z

~

`

2---

`đ --s

đ =Soil Sample
Figure 6. Soil sampling pattern for row crops.


1 re
= 2
-

et
——sos

-

ad

ees,

Soil Sample

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.—

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